pSL891

LOCUS       pSL891        7705 bp DNA     circular SYN 14-JAN-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7705)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Jul 21, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7705
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    232
                     /label=CMV
     promoter        245..441
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     regulatory      467..476
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    473..535
                     /label=Igk secretion signal
     CDS             541
                     /codon_start=1
                     /label=linker
                     /translation=""
     CDS             542..1240
                     /codon_start=1
                     /label=GNCR1-N
                     /translation="DIEKLCKKAEEEAKEAQEKADELRQRHPDSQAAEDAEDLANLAVA
                     AVLTACLLAQEHPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQAA
                     RAVILAIMLAAENPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQA
                     AEAVERAIWLAAENPNADIAKKCIKAASEAAEEASKAAEEAQRHPDSQKARDEIKEASQ
                     KAEEVKERCKS"
     misc_feature    1247..1990
                     /label=mouse EpoR-D1_D2_TM
                     /note="mouse EpoR-D1_D2_TM"
     primer_bind     complement(1314..1336)
                     /label=186-WKG-603-R
     misc_feature    1520..1522
                     /label=F93A
                     /note="F93A"
     CDS             1991..3261
                     /codon_start=1
                     /label=mFGFR 405-end
                     /note="mFGFR 405-end"
                     /translation="MKSGTKKSDFHSQMAVHKLAKSIPLRRQVTVSADSSASMNSGVLL
                     VRPSRLSSSGTPMLAGVSEYELPEDPRWELPRDRLVLGKPLGEGCFGQVVLAEAIGLDK
                     DKPNRVTKVAVKMLKSDATEKDLSDLISEMEMMKMIGKHKNIINLLGACTQDGPLYVIV
                     EYASKGNLREYLQARRPPGLEYCYNPSHNPEEQLSSKDLVSCAYQVARGMEYLASKKCI
                     HRDLAARNVLVTEDNVMKIADFGLARDIHHIDYYKKTTNGRLPVKWMAPEALFDRIYTH
                     QSDVWSFGVLLWEIFTLGGSPYPGVPVEELFKLLKEGHRMDKPSNCTNELYMMMRDCWH
                     AVPSQRPTFKQLVEDLDRIVALTSNQEYLDLSIPLDQYSPSFPDTRSSTCSSGEDSVFS
                     HEPLPEEPCLPRHPTQLANSGLKR"
     misc_feature    2825..2827
                     /label=Y677
                     /note="Y677"
     polyA_signal    3302..3526
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3572..4000
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3586..3610)
                     /label=184-WKG-599-R
     primer_bind     3871..3890
                     /label=189-WKG-612-F
     promoter        4014..4343
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             4410..5204
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4454..4481)
                     /label=188-WKG-610-R
     polyA_signal    5378..5499
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5544..5564)
                     /label=M13-rev
     primer_bind     complement(5548..5564)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_binding    complement(5570..5592)
                     /label=LacO
     protein_bind    5572..5588
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5596..5626)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     promoter        complement(5597..5626)
                     /label=lac
     protein_bind    5641..5662
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    5768..6708
                     /label=ColE1
                     /note="ColE1"
     CDS             complement(6709..7569)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7570..7674)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtgatctgc
      241 gatctgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
      301 ctaactccgc ccagttccgc ccattctccg ccccatcgct gactaatttt ttttatttat
      361 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
      421 ggaggcctag gcttttgcaa aaagctacga atcgcgctag tggccggcca ccatggagac
      481 cgatacctta ttactgtggg tgcttcttct gtgggttccc ggtagcaccg gagacggatc
      541 cgacatcgag aagctgtgca agaaggcgga agaggaggcc aaggaggccc aggagaaggc
      601 cgacgagctg agacaacgac accccgattc gcaggcggcg gaagacgcgg aggatctcgc
      661 aaatctggct gtcgccgccg tgctgaccgc ttgtctgctg gcccaggagc atcccaacgc
      721 cgatatcgct aagctgtgca tcaaggccgc gagcgaagct gcggaggccg cgtcaaaagc
      781 cgccgagctg gcccaacggc acccagacag tcaagcagcc agagatgcta tcaaactcgc
      841 ctctcaagcc gcccgcgccg tgatcctggc catcatgctc gccgcagaga atcccaacgc
      901 tgatattgca aagctatgta ttaaggcggc cagcgaagcc gcagaggctg cctcgaaagc
      961 tgcggaactt gcacagcggc accccgactc ccaggccgcc cgagatgcaa ttaaactggc
     1021 cagtcaggcc gctgaggcgg tggagagagc catctggctg gccgccgaga accccaatgc
     1081 cgatatcgcc aaaaaatgca tcaaagcggc ctccgaagca gccgaggagg cctctaaagc
     1141 tgccgaagag gctcaaagac acccagacag ccagaaggca cgcgacgaga tcaaggaggc
     1201 ctctcagaag gccgaggagg tgaaggagag atgcaagagc gaattcgcac cttcacccag
     1261 cctcccggac cccaagtttg agagcaaagc ggccctgctg gcatcccggg gctccgaaga
     1321 acttctgtgc ttcacccaac gcttggaaga cttggtgtgt ttctgggagg aagcggcgag
     1381 ctccgggatg gacttcaact acagcttctc ataccagctc gagggtgagt cacgaaagtc
     1441 atgtagcctg caccaggctc ccaccgtccg cggctccgtg cgtttctggt gttcactgcc
     1501 aacagcggac acatcgagtg ctgtgccgct ggagctgcag gtgacggagg cgtccggttc
     1561 tcctcgctat caccgcatca tccatatcaa tgaagtagtg ctcctggacg cccccgcggg
     1621 gctgctggcg cgccgggcag aagagggcag ccacgtggtg ctgcgctggc tgccacctcc
     1681 tggagcacct atgaccaccc acatccgata tgaagtggac gtgtcggcag gcaaccgggc
     1741 aggagggaca caaagggtgg aggtcctgga aggccgcact gagtgtgttc tgagcaacct
     1801 gcggggcggg acgcgctaca ccttcgctgt tcgagcgcgc atggccgagc cgagcttcag
     1861 cggattctgg agtgcctggt ctgagcccgc gtcactactg accgctagcg acctggaccc
     1921 tctcatcttg acgctgtctc tcattctggt cctcatctcg ttgttgctga cggttctggc
     1981 cctgctgtcc atgaagagcg gcaccaagaa gagcgacttc catagccaga tggctgtgca
     2041 caagctggcc aagagcatcc ctctgcgcag acaagtaaca gtgtcagctg actccagtgc
     2101 atccatgaac tctggggttc tcctggttcg gccctcacgg ctctcctcca gcgggacccc
     2161 catgctggct ggagtctccg aatatgagct ccctgaggac ccccgctggg agctgccacg
     2221 agacagactg gtcttaggca aaccacttgg cgagggctgc ttcgggcagg tggtgttggc
     2281 tgaggccatc gggctggata aggacaaacc caaccgtgtg accaaagtgg ccgtgaagat
     2341 gttgaagtcc gacgcaacgg agaaggacct gtcggatctg atctcggaga tggagatgat
     2401 gaaaatgatt gggaagcaca agaatatcat caaccttctg ggagcgtgca cacaggatgg
     2461 tcctctttat gtcattgtgg agtacgcctc caaaggcaat ctccgggagt atctacaggc
     2521 ccggaggcct cctgggctgg agtactgcta taaccccagc cacaaccccg aggaacagct
     2581 gtcttccaaa gatctggtat cctgtgccta tcaggtggct cggggcatgg agtatcttgc
     2641 ctctaagaag tgtatacacc gagacctggc tgctaggaac gtcctggtga ccgaggataa
     2701 cgtaatgaag atcgcagact ttggcttagc tcgagacatt catcatatcg actactacaa
     2761 gaaaaccacc aacggccggc tgcctgtgaa gtggatggcc cctgaggcgt tgtttgaccg
     2821 gatctacaca caccagagcg atgtgtggtc ttttggagtg ctcttgtggg agatcttcac
     2881 tctgggtggc tccccatacc ccggtgtgcc tgtggaggaa cttttcaagc tgctgaagga
     2941 gggtcatcga atggacaagc ccagtaactg taccaatgag ctgtacatga tgatgcggga
     3001 ctgctggcat gcagtgccct ctcagagacc tacgttcaag cagttggtgg aagacctgga
     3061 ccgcattgtg gccttgacct ccaaccagga gtatctggac ctgtccatac cgctggacca
     3121 gtactcaccc agctttcccg acacacggag ctccacctgc tcctcagggg aggactctgt
     3181 cttctctcat gagccgttac ctgaggagcc ctgtctgcct cgacacccca cccagcttgc
     3241 caacagtgga ctcaaacgcg ctagtctaga gggcccgttt aaacccgctg atcagcctcg
     3301 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     3361 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     3421 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     3481 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     3541 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     3601 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     3661 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     3721 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     3781 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     3841 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     3901 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     3961 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
     4021 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     4081 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     4141 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     4201 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     4261 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     4321 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
     4381 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
     4441 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     4501 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     4561 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
     4621 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     4681 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     4741 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     4801 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     4861 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     4921 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     4981 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     5041 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     5101 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     5161 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
     5221 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
     5281 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     5341 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
     5401 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     5461 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
     5521 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     5581 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     5641 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     5701 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     5761 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     5821 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     5881 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     5941 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     6001 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     6061 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     6121 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     6181 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     6241 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     6301 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     6361 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     6421 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     6481 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     6541 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     6601 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     6661 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     6721 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     6781 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     6841 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     6901 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     6961 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     7021 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     7081 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     7141 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     7201 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     7261 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     7321 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     7381 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     7441 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     7501 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     7561 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     7621 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     7681 tccccgaaaa gtgccacctg acgtc
//