| 质粒名称: | pLH-sgRNA1-2XboxB |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 75391 |
| 存储实验室: | Thoru Pederson |
|
载体骨架基本信息 |
|
| 载体名称: | pLKO.1 |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 8000 bp |
| 完整质粒大小: | 8313 bp |
| 载体类型: | 哺乳动物表达，慢病毒，CRISPR |
| 筛选标记: | 潮霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | sgRNA1-2XboxB |
| 物种来源: | Synthetic |
| 插入大小: | 106 bp |
| 启动子 | U6 |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | Sbf I(未破坏) |
| 3' 克隆位点: | EcoR I(未破坏) |
| 5' 测序引物 | cccttcaccgagggcctatttccc |
| 3' 测序引物 | CTATTCTTTCCCCTGCACTGTACCC |
|
文献引用方法 |
|
| 材料和方法部分: | pLH-sgRNA1-2XboxB was a gift from Thoru Pederson (Addgene plasmid # 75391 ; http://n2t.net/addgene:75391 ; RRID:Addgene_75391) |
| 参考文献部分: | Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow. Ma H, Tu LC, Naseri A, Huisman M, Zhang S, Grunwald D, Pederson T. Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526. 10.1038/nbt.3526 PubMed 27088723 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLH-sgRNA1-2XboxB 8297 bp ds-DNA circular SYN 02-MAY-2019
DEFINITION sgRNA1-2XboxB.
ACCESSION .
VERSION .
KEYWORDS pLH-sgRNA1-2XboxB
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8297)
AUTHORS Ma H, Tu LC, Naseri A, Huisman M, Zhang S, Grunwald D, Pederson T
TITLE Multiplexed labeling of genomic loci with dCas9 and engineered
sgRNAs using CRISPRainbow.
JOURNAL Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526.
PUBMED 27088723
REFERENCE 2 (bases 1 to 8297)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 2, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8297
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind 106..127
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 142..172
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 180..196
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 185..207
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 204..220
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 204..220
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 239..259
/label=T3
/note="T3 promoter, forward primer"
promoter 241..259
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 287..513
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 514..694
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 741..866
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1359..1592
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
promoter 2119..2359
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA"
primer_bind 2119..2139
/label=hU6-F
/note="Human U6 promoter, forward primer"
primer_bind 2290..2309
/label=LKO.1 5'
/note="Human U6 promoter, forward primer"
CDS 2708..3013
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/label=ccdB
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
primer_bind 2892..2911
/label=ccdB-fwd
/note="ccdB gene, forward primer"
misc_feature 3188..3305
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 3354..3864
/label=hPGK promoter
/note="human phosphoglycerate kinase 1 promoter"
primer_bind 3783..3801
/label=hPGK-F
/note="Human PGK promoter, forward primer"
CDS 3954..4979
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
E"
primer_bind complement(5003..5019)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5004..5020)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 5102..5335
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 5407..5528
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5444..5463)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 5498..5517
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 5568..5703
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 5630..5649
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(5723..5742)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(5724..5742)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(5752..5769)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(5752..5768)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5761..5783)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 5910..6365
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5997..6016)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6207..6228
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 6391..6495
/gene="bla"
/label=AmpR promoter
CDS 6496..7356
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(6714..6733)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 7527..8115
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 8016..8035
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 8269..8286
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc
241 aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca
301 atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag
361 aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga
421 aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat
481 attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga
541 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
601 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
661 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
721 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
781 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
841 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
901 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
961 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
1021 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1081 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1141 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1201 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
1261 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
1321 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
1381 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
1441 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
1501 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
1561 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
1621 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
1681 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
1741 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
1801 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
1861 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
1921 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
1981 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
2041 gacagatcca ttcgattagt gaacggatct cgacggtatc gatcacgaga ctagcctcga
2101 gcggccgccc ccttcaccga gggcctattt cccatgattc cttcatattt gcatatacga
2161 tacaaggctg ttagagagat aattggaatt aatttgactg taaacacaaa gatattagta
2221 caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt
2281 tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta
2341 tatatcttgt ggaaaggacg aaacaccggt gtcttcgagg cttactaaaa gccagataac
2401 agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat atgtataccc
2461 gaagtatgtc aaaaagaggt gtgctatgaa gcagcgtatt acagtgacag ttgacagcga
2521 cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt aagcacaacc
2581 atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg
2641 atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga gaacagggac
2701 tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt
2761 ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc ccctggccag
2821 tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc atatcgggga
2881 tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg ttatcgggga
2941 agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt
3001 ctggggaata taaaccctgc aggacgaaga cccgtttgag agctagggcc ctgaagaagg
3061 gccctagcaa gttcaaataa ggctagtccg ttatcaactt gggccctgaa gaagggccca
3121 agtggcaccg agtcggtgct ttttttgaat tctcgacctc gagacaaatg gcagtattca
3181 tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
3241 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
3301 attttcgggt ttattacagg gacagcagag atccactttg gccgcggctc gagggggttg
3361 gggttgcgcc ttttccaagg cagccctggg tttgcgcagg gacgcggctg ctctgggcgt
3421 ggttccggga aacgcagcgg cgccgaccct gggtctcgca cattcttcac gtccgttcgc
3481 agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc cggcgacgct tcctgctccg
3541 cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc
3601 gcacgtctca ctagtaccct cgcagacgga cagcgccagg gagcaatggc agcgcgccga
3661 ccgcgatggg ctgtggccaa tagcggctgc tcagcagggc gcgccgagag cagcggccgg
3721 gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt gggccctgtt cctgcccgcg
3781 cggtgttccg cattctgcaa gcctccggag cgcacgtcgg cagtcggctc cctcgttgac
3841 cgaatcaccg acctctctcc ccagggggat ccaccggatc aagcttgcca gctggggcgc
3901 cctctggtaa ggttgggaag ccctgcagat ctgatcccgg ggggcaatga gatatgaaaa
3961 agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct
4021 ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag
4081 ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg
4141 tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat
4201 ttagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagatc
4261 tgcctgaaac cgaactgccc gctgttctgc agccggtcgc ggaggccatg gatgcgatcg
4321 ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc
4381 aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc
4441 aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc
4501 tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca
4561 atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg
4621 gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg
4681 agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcggctcc
4741 gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt
4801 tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga
4861 ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag
4921 aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca aaggaataga
4981 gtagatgccg accgggatct atcgataccg tcgacctcga gggggggccc ggtaccttta
5041 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
5101 ctggaagggc taattcactc ccaacgaaga aaagatctgc tttttgcttg tactgggtct
5161 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
5221 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
5281 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
5341 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
5401 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
5461 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
5521 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa
5581 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
5641 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
5701 gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
5761 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
5821 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
5881 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
5941 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
6001 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
6061 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
6121 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
6181 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
6241 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
6301 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
6361 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
6421 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
6481 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
6541 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
6601 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
6661 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
6721 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
6781 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
6841 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
6901 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
6961 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
7021 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
7081 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
7141 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
7201 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
7261 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
7321 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
7381 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
7441 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
7501 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
7561 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
7621 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
7681 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
7741 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
7801 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
7861 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
7921 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
7981 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
8041 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
8101 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
8161 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
8221 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
8281 agcgaggaag cggaaga
//
