pDG137

LOCUS       pDG137             7052 bp DNA     circular SYN 10-DEC-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7052)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 18, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7052
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    13..38
                     /label=SleepingBeauty-ITR-Li
     misc_feature    209..240
                     /label=SleepingBeauty-ITR_Li
     primer_bind     276..317
                     /label=oMH09-pWS252-EF1a-
                     /note="AGGGGTCCGCTATCTACGGGAAAGTGATGTCGTGTAC 同源臂"
     misc_feature    298..1364
                     /label=P_EF1a
     intron          417..1355
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(1335..1365)
                     /label=Fragment.REV
     intron          1373..1505
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     misc_feature    1373..1505
                     /label=CI
     CDS             1553..2587
                     /codon_start=1
                     /product="Mus musculus olfactory receptor MOR9-1 gene, 
                     complete cds
                     GenBank: AY073004.1"
                     /label=MOR9-1
                     /translation="MNGTEGPNFYVPFSNATGVVREFTRMSYSNHSSTSFFLTGLPGLE
                     TVYLWLSIPLCTMYIASLAGNGLILWVVKSEPSLHQPMYYFLSMLAVTDLGLSVSTLPT
                     MLTIYMMGVSEVALDMCLAQLFFIHTFSIMESSVLLTMAFDRVVAISSPLHYATILTNP
                     RVASLGMVILVRSIGLHIPAPIMLKKLPYCQKRHLSHSYCLHPDVMKLACTDTRINSAY
                     GLFVVLSTLGVDSVLIVLSYGLILYTVLSIASKTERLKALNTCVSHICSVLLFYTPMIG
                     LSMIHRFGKWASPCSRVLLSYLHFLTPPVLNPVVYTIKTKQIRQRIWCIFRCGGRSIGH
                     IQGH"
     primer_bind     complement(2588..2604)
                     /label=Fragment.REV
     polyA_signal    2595..2785
                     /label=SV40 late polyA
     promoter        2799..3298
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             3319..3690
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASVPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     CDS             3700..3756
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3748..3750
                     /codon_start=1
                     /label=Feature 30
                     /translation="P"
     CDS             3763..4470
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     CDS             4471
                     /codon_start=1
                     /label=linker
                     /translation=""
     terminator      4496..4723
                     /label=bGH PA
     misc_feature    4807..4835
                     /label=SleepingBeauty-ITR_Ri
     misc_feature    5001..5032
                     /label=SleepingBeauty-ITR_Ro
     rep_origin      complement(5202..5830)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(5979..6839)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6840..6944)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        complement(6881..6909)
                     /label=Amp prom
ORIGIN
        1 ggatccctat acagttgaag tcggaagttt acatacactt aagttggagt cattaaaact
       61 cgtttttcaa ctactccaca aatttcttgt taacaaacaa tagttttggc aagtcagtta
      121 ggacatctac tttgtgcatg acacaagtca tttttccaac aattgtttac agacagatta
      181 tttcacttat aattcactgt atcacaattc cagtgggtca gaagtttaca tacactaagt
      241 tcgactcctc tgcagaatgc ggcgatgttt cggtaagggg tccgctatct actcgacggg
      301 aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa
      361 gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa
      421 gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt
      481 gaattacttc cacctggctg cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg
      541 ggtgggagag ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct tgagttgagg
      601 cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg
      661 ctgctttcga taagtctcta gccatttaaa atttttgatg acctgctgcg acgctttttt
      721 tctggcaaga tagtcttgta aatgcgggcc aagatctgca cactggtatt tcggtttttg
      781 gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc
      841 tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctggccgg cctgctctgg
      901 tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg
      961 caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctgcaggg agctcaaaat
     1021 ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct
     1081 ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg tccaggcacc
     1141 tcgattagtt ctcgagcttt tggagtacgt cgtctttagg ttggggggag gggttttatg
     1201 cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct tggcacttga
     1261 tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt ctcaagcctc
     1321 agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtgacctgca gggtaagtat
     1381 caaggttaca agacaggttt aaggagacca atagaaactg ggcttgtcga gacagagaag
     1441 actcttgcgt ttctgatagg cacctattgg tcttactgac atccactttg cctttctctc
     1501 cacagggtac cgagctcgga tccactagtc cagtgtggtg gaattcgcca ccatgaatgg
     1561 cacagaaggc cctaacttct acgtgccctt ctccaatgcg acgggtgtgg tacgcgaatt
     1621 cacgcgtatg tcctactcca atcattccag cacctcattc tttcttactg gcctccctgg
     1681 ccttgagaca gtgtatctct ggctctccat tcctttgtgc accatgtaca ttgcctctct
     1741 ggcagggaat ggcttgattc tgtgggttgt aaagtcagag ccctccctgc accagcctat
     1801 gtactacttt ctatctatgc ttgcagttac tgacctcggc ctgtctgtct ccacactgcc
     1861 taccatgctg acgatctata tgatgggtgt cagcgaagtg gcattagaca tgtgccttgc
     1921 acagctcttc ttcatccata ctttctccat catggagtca tctgtgctgc tgactatggc
     1981 ctttgaccgt gttgtggcca tcagcagtcc cctacactat gccaccatcc tcaccaaccc
     2041 tcgggttgcc agtttgggca tggtcatttt ggtacgaagc attggtctcc acatccctgc
     2101 ccccatcatg ctgaagaagc taccttattg ccagaagcgt catctttccc actcttactg
     2161 cctgcaccca gatgttatga agctggcctg tactgacacc cgcatcaaca gtgcctatgg
     2221 cctctttgtg gttctctcta ctctgggtgt ggactctgtg ctcatcgttc tatcctatgg
     2281 gctgatcctc tacacagtgc tgtcaatcgc ttccaagact gaacgcctca aagccctcaa
     2341 cacctgtgtt tcccacatct gttctgtgct gctcttctac acacctatga ttggcctttc
     2401 tatgatccac cgatttggca agtgggcatc cccctgcagc cgtgtgttgc tctcttatct
     2461 tcactttctc acacctccag tgctcaaccc agttgtttat accataaaga ccaagcagat
     2521 ccgacagagg atttggtgca tcttccggtg tggtggaaga agcattgggc atatccaggg
     2581 tcactaagac gcgtcagaca tgataagata cattgatgag tttggacaaa ccacaactag
     2641 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     2701 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
     2761 tcagggggag gtgtgggagg tttttagctt gtctgtccgg gtaggggagg cgcttttccc
     2821 aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg
     2881 cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg
     2941 tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca
     3001 gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat
     3061 ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct
     3121 ttgctccttc gctttctggg ctcagaggct gggaatgggt gggtccgggg gcgggctcag
     3181 gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac
     3241 gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac
     3301 ctgcagccca agcttaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc
     3361 gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg
     3421 gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag
     3481 gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg
     3541 tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgt gccggccatg
     3601 accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac
     3661 tgcgtgcact tcgtggccga ggagcaggac ggaagcggag ctactaactt cagcctgctg
     3721 aagcaggctg gagacgtgga ggagaaccct ggaccttccg gaatggtgag caagggcgag
     3781 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc
     3841 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
     3901 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg
     3961 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac
     4021 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     4081 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag
     4141 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg
     4201 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc
     4261 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac
     4321 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc
     4381 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac
     4441 tccaccggcg gcatggacga gctgtacaag tgatctagag ctcgctgatc agcctcgact
     4501 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     4561 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     4621 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     4681 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     4741 accagctggg cctactagct actcgggacc ccttaccgaa acatcgccgc attctgcaga
     4801 ggagtcgagt gtatgtaaac ttctgaccca ctgggaatgt gatgaaagaa ataaaagctg
     4861 aaatgaatca ttctctctac tattattctg atatttcaca ttcttaaaat aaagtggtga
     4921 tcctaactga cctaagacag ggaattttta ctaggattaa atgtcaggaa ttgtgaaaaa
     4981 gtgagtttaa atgtatttgg ctaaggtgta tgtaaacttc cgacttcaac tgtataggga
     5041 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     5101 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     5161 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     5221 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     5281 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     5341 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     5401 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     5461 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     5521 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     5581 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     5641 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     5701 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     5761 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     5821 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     5881 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     5941 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     6001 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     6061 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     6121 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     6181 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     6241 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     6301 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     6361 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     6421 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     6481 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     6541 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     6601 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     6661 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     6721 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     6781 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     6841 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     6901 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     6961 gtgccacctg atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
     7021 gaaattgtaa gcgttaatat tttgttaaaa tt
//