| 质粒名称: | pCDH-EF1a-mCherry-EGFP-LC3B |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 170446 |
| 存储实验室: | Sang-Hun Lee |
|
载体骨架基本信息 |
|
| 载体名称: | pCDH-EF1a-T2A-copGFP |
| 载体出品公司: | System Biosciences |
| 空载体大小: | 6607 bp |
| 完整质粒大小: |
8533 bp
|
| 载体修饰: | -- |
| 载体类型: | 慢病毒载体 |
| 筛选标记: | -- |
| 载体抗性: | 氨苄青霉素，100μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | mCherry-GFP-LC3 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 1926 bp |
| 基因突变: | -- |
| GenBank编号 | NM_022818.5 |
| 启动子: | EF1a |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | BamHI (未破坏) |
| 3’克隆位点: | SalI (未破坏) |
| 5’测序引物 | GGATCTCGACGGTATCGG |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pCDH-EF1a-mCherry-EGFP-LC3B was a gift from Sang-Hun Lee (Addgene plasmid # 170446 ; http://n2t.net/addgene:170446 ; RRID:Addgene_170446) |
| 参考文献部分: | Neurodevelopmental defects and neurodegenerative phenotypes in human brain organoids carrying Parkinson's disease-linked DNAJC6 mutations. Wulansari N, Darsono WHW, Woo HJ, Chang MY, Kim J, Bae EJ, Sun W, Lee JH, Cho IJ, Shin H, Lee SJ, Lee SH. Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabb1540. doi: 10.1126/sciadv.abb1540. Print 2021 Feb. 10.1126/sciadv.abb1540 PubMed 33597231 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCDH-EF1a-mCherry-EGFP-LC3B 8368 bp ds-DNA circular SYN 16-JUN-2021
DEFINITION Lentiviral vector expressing tandem mCherry-EGFP-LC3B in mamalian
cell. To visualize free autophagosome and autophagosomes that have
fused with the lysosome..
ACCESSION .
VERSION .
KEYWORDS pCDH-EF1a-mCherry-EGFP-LC3B
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8368)
AUTHORS Wulansari N, Darsono WHW, Woo HJ, Chang MY, Kim J, Bae EJ, Sun W,
Lee JH, Cho IJ, Shin H, Lee SJ, Lee SH
TITLE Neurodevelopmental defects and neurodegenerative phenotypes in human
brain organoids carrying Parkinson's disease-linked DNAJC6
mutations.
JOURNAL Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabb1540. doi:
10.1126/sciadv.abb1540. Print 2021 Feb.
PUBMED 33597231
REFERENCE 2 (bases 1 to 8368)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jun 16, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..8368
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 6..232
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 233..413
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 460..585
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1078..1311
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1496..1540
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 1807..1923
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 1966..2177
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 2190..2458
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
regulatory 2519..2528
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 2525..3232
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
primer_bind complement(2670..2688)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind 2938..2957
/label=mCherry-F
/note="mCherry, forward primer"
protein_bind 3260..3284
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
regulatory 3293..3302
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 3299..4015
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS 3299..4015
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(3344..3365)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(3605..3624)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 3952..3973
/label=EGFP-C
/note="EGFP, forward primer"
CDS 4043..4417
/codon_start=1
/gene="MAP1LC3B"
/product="human LC3 protein"
/label=LC3B
/note="Atg8 family member found on autophagosomes"
/translation="MPSEKTFKQRRTFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLP
VLDKTKFLVPDHVNMSELIKIIRRRLQLNANQAFFLLVNGHSMVSVSTPISEVYESEKD
EDGFLYMVYASQETFGMKLSV"
misc_feature 4447..5035
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(4500..4520)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(4918..4929)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 5109..5342
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 5414..5535
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5451..5470)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 5505..5524
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 5575..5710
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind complement(5743..5759)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5743..5759)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(5756..5778)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 5767..5783
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5791..5821)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5836..5857
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(5974..5991)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6145..6733)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6225..6244)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(6904..7764)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7527..7546
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7765..7869)
/gene="bla"
/label=AmpR promoter
primer_bind 7937..7955
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(7993..8015)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 8115..8134
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 8328..8350
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 8342..8359
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 8343..8359
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
901 agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag
961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
1801 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat
1921 tttatcgata ctagtggatc tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat
1981 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt gcctagagaa
2041 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg
2101 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt
2161 ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca cgcgcccgcc
2221 gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg
2281 gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg agaccgggcc
2341 tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg ctttgcctga
2401 ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt tacagatcca
2461 agctgtgacc ggcgcctact ctagagctag cgaattcgaa tttaaatcgg atccggtcgc
2521 caccatggtg agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt
2581 caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga
2641 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct
2701 gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa
2761 gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga
2821 gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca
2881 ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc
2941 cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga
3001 cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga
3061 cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa
3121 cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta
3181 cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca agtccggact
3241 cagatccgct ggcccaggaa caagtttgta caaaaaagca ggctttccgg tcgccaccat
3301 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
3361 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
3421 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
3481 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
3541 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
3601 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
3661 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
3721 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
3781 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
3841 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
3901 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
3961 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtccgg
4021 actcagatcc ggtaccgaat tcatgccgtc ggagaagacc ttcaagcagc gccgcacctt
4081 cgaacaaaga gtagaagatg tccgacttat tcgagagcag catccaacca aaatcccggt
4141 gataatagaa cgatacaagg gtgagaagca gcttcctgtt ctggataaaa caaagttcct
4201 tgtacctgac catgtcaaca tgagtgagct catcaagata attagaaggc gcttacagct
4261 caatgctaat caggccttct tcctgttggt gaacggacac agcatggtca gcgtctccac
4321 accaatctca gaggtgtatg agagtgagaa agatgaagat ggattcctgt acatggtcta
4381 tgcctcccag gagacgttcg ggatgaaatt gtcagtgtaa gtcgagcatg catctagacc
4441 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
4501 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
4561 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
4621 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
4681 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
4741 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
4801 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
4861 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
4921 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
4981 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctggt
5041 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
5101 ggggggactg gaagggctaa ttcactccca acgaagataa gatctgcttt ttgcttgtac
5161 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
5221 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
5281 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
5341 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
5401 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
5461 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
5521 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg
5581 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
5641 tatgcagagg cagaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
5701 tttggaggcc tagacttttg cagagaccaa attcgtaatc atgtcatagc tgtttcctgt
5761 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
5821 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
5881 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
5941 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
6001 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
6061 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
6121 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
6181 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
6241 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
6301 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
6361 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
6421 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
6481 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
6541 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
6601 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
6661 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
6721 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
6781 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
6841 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
6901 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
6961 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
7021 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
7081 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
7141 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
7201 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
7261 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
7321 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
7381 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
7441 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
7501 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
7561 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
7621 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
7681 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
7741 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
7801 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
7861 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
7921 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga
7981 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
8041 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
8101 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
8161 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct
8221 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
8281 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
8341 ttgtaaaacg acggccagtg ccaagctg
//
