pRetroX-TRE3G

价格:3500元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Clontech
载体名称: pRetroX-TRE3G
质粒类型: 逆病毒载体;四环素调控载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: TRE3GV
载体大小: 6600 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签:
载体抗性: 氨苄青霉素
筛选标记: 嘌呤霉素(Puromycin)
克隆菌株: DH5α
宿主细胞(系): 常规细胞系(293、CV-1、CHO等)
备注: 逆病毒载体pRetroX-TRE3G是四环素调控系统的应答载体,需要与相应的调控载体pRetroX-Tet3G共同使用。
产品目录号: --
稳定性: 稳表达
组成型/诱导型: 诱导型
病毒/非病毒: 逆转录病毒

载体质粒图谱和多克隆位点信息

pRetroX-TRE3G载体图谱



pRetroX-TRE3G 多克隆位点

pRetroX-TRE3G 载体特征

载体简介

载体序列

LOCUS       pRetroX-TRE3G	6600 bp 	DNA	SYN
DEFINITION  pRetroX-TRE3G
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..6600
                     /organism="pRetroX-TRE3G"
                     /mol_type="other DNA"
     promoter        2..507
                     /label="CMV_immearly_promoter"
     misc_feature    6..689
                     /label="5_LTR"
     misc_feature    9..297
                     /label="CAG_enhancer"
     misc_feature    464..484
                     /label="CMV_fwd_primer"
     misc_feature    759..1568
                     /label="psi_plus_pack"
     misc_feature    1155..1562
                     /label="gag"
     misc_feature    1498..1520
                     /label="MSCV_primer"
     misc_feature    1498..1520
                     /label="pLXSN_5_primer"
     misc_feature    1536..1552
                     /label="pBABE_5_primer"
     misc_feature    1894..1913
                     /label="pCEP_fwd_primer"
     misc_feature    1896..1920
                     /label="LNCX_primer"
     misc_feature    complement(2041..2064)
                     /label="MSCV_rev_primer"
     CDS             2286..3125
                     /label="ORF frame 3"
                     /translation="MEAGRPLGQRPIAALLLRFLGSEAGKGWVRGRAQGRAQGRGGRP
                     KVLRRPGILHASKAHVCRAVLLFLISGPFDLQPKLTMTEYKPTVRLATRDDVPRAVRT
                     LAAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPE
                     SVEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKG
                     LGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRK
                     PGA*"
     misc_feature    2438..2457
                     /label="mPGK_F_primer"
                     /translation="MEAGRPLGQRPIAALLLRFLGSEAGKGWVRGRAQGRAQGRGGRP
                     KVLRRPGILHASKAHVCRAVLLFLISGPFDLQPKLTMTEYKPTVRLATRDDVPRAVRT
                     LAAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPE
                     SVEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKG
                     LGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRK
                     PGA*"
     CDS             complement(2451..3176)
                     /label="ORF frame 2"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLGLQVERPGDEEEENSAADVRF*"
     gene            2526..3125
                     /label="puro (variant)"
                     /gene="puro (variant)"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLGLQVERPGDEEEENSAADVRF*"
     misc_feature    complement(3834..3856)
                     /label="pGEX_3_primer"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLGLQVERPGDEEEENSAADVRF*"
     misc_feature    complement(3992..4012)
                     /label="pBABE_3_primer"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLGLQVERPGDEEEENSAADVRF*"
     misc_feature    complement(3998..4213)
                     /label="SV40_enhancer"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLGLQVERPGDEEEENSAADVRF*"
     promoter        4010..4278
                     /label="SV40_promoter"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLGLQVERPGDEEEENSAADVRF*"
     rep_origin      4177..4254
                     /label="SV40_origin"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLGLQVERPGDEEEENSAADVRF*"
     misc_feature    4239..4258
                     /label="SV40pro_F_primer"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLGLQVERPGDEEEENSAADVRF*"
     gene            complement(5378..6238)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLGLQVERPGDEEEENSAADVRF*"
     CDS             complement(5378..6238)
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(6280..6308)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        6530..507
                     /label="CMV_immearly_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    6594..689
                     /label="5_LTR"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
   61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
  121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
  181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgctctg gcattatgcc
  241 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
  301 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
  361 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
  421 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
  481 cgtgtacggt gggaggtcta tataagcaga gctcaataaa agagcccaca acccctcact
  541 cggcgcgcca gtcttccgat agactgcgtc gcccgggtac ccgtattccc aataaagcct
  601 cttgctgttt gcatccgaat cgtggtctcg ctgttccttg ggagggtctc ctctgagtga
  661 ttgactaccc acgacggggg tctttcattt gggggctcgt ccgggatttg gagacccctg
  721 cccagggacc accgacccac caccgggagg taagctggcc agcaacttat ctgtgtctgt
  781 ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg tctgtactag ttagctaact
  841 agctctgtat ctggcggacc cgtggtggaa ctgacgagtt ctgaacaccc ggccgcaacc
  901 ctgggagacg tcccagggac tttgggggcc gtttttgtgg cccgacctga ggaagggagt
  961 cgatgtggaa tccgaccccg tcaggatatg tggttctggt aggagacgag aacctaaaac
 1021 agttcccgcc tccgtctgaa tttttgcttt cggtttggaa ccgaagccgc gcgtcttgtc
 1081 tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct gactgtgttt ctgtatttgt
 1141 ctgaaaatta gggccagact gttaccactc ccttaagttt gaccttaggt cactggaaag
 1201 atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa gaagagacgt tgggttacct
 1261 tctgctctgc agaatggcca acctttaacg tcggatggcc gcgagacggc acctttaacc
 1321 gagacctcat cacccaggtt aagatcaagg tcttttcacc tggcccgcat ggacacccag
 1381 accaggtccc ctacatcgtg acctgggaag ccttggcttt tgacccccct ccctgggtca
 1441 agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgccccg tctctccccc
 1501 ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc actccttctc
 1561 taggcgccgg aattgaagat ctcgatgagg ccctttcgtc ttcactcgag tttactccct
 1621 atcagtgata gagaacgtat gaagagttta ctccctatca gtgatagaga acgtatgcag
 1681 actttactcc ctatcagtga tagagaacgt ataaggagtt tactccctat cagtgataga
 1741 gaacgtatga ccagtttact ccctatcagt gatagagaac gtatctacag tttactccct
 1801 atcagtgata gagaacgtat atccagttta ctccctatca gtgatagaga acgtatgtcg
 1861 aggtaggcgt gtacggtggg cgcctataaa agcagagctc gtttagtgaa ccgtcagatc
 1921 gcctggagca attccacaac acttttgtct tatacttgga tccatcgata cgcgtgcggc
 1981 cgcgccggcg ggcccgaatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga
 2041 gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc
 2101 acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg
 2161 ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc
 2221 aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct
 2281 gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct
 2341 ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg
 2401 ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc
 2461 acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg cagcccaagc
 2521 ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg
 2581 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc
 2641 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
 2701 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
 2761 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
 2821 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
 2881 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
 2941 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
 3001 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
 3061 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
 3121 cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatgcat
 3181 cggcgtctcg agatatcagt ggtccaggct ctagttttga ctcaacaata tcaccagctg
 3241 aagcctatag agtacgagcc atagataaaa taaaagattt tatttagtct ccagaaaaag
 3301 gggggaatga aagaccccac ctgtaggttt ggcaagctag cttaagtaac gccattttgc
 3361 aaggcatgga aaaatacata actgagaata gagaagttca gatcaaggtc aggaacagat
 3421 ggaacagggt cgaccctaga gaaccatcag atgtttccag ggtgccccaa ggacctgaaa
 3481 tgaccctgtg ccttatttga actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct
 3541 tctgctcccc gagctcaata aaagagccca caacccctca ctcggggcgc cagtcctccg
 3601 attgactgag tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt tgcatccgac
 3661 ttgtggtctc gctgttcctt gggagggtct cctctgagtg attgactacc cgtcagcggg
 3721 ggtctttcat ttgggggctc gtccgggatc gggagacccc tgcccaggga ccaccgaccc
 3781 accaccggga ggtaagctgg ctgcctcgcg cgtttcggtg atgacggtga aaacctctga
 3841 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
 3901 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca
 3961 cgtagcgata gcggagtgta gatccggctg tggaatgtgt gtcagttagg gtgtggaaag
 4021 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
 4081 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
 4141 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
 4201 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
 4261 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
 4321 tgcaaaaagc ttactggctt aactatgcgg catcagagca gattgtactg agagtgcacc
 4381 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt
 4441 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
 4501 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
 4561 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
 4621 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
 4681 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
 4741 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
 4801 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
 4861 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
 4921 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
 4981 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
 5041 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
 5101 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
 5161 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
 5221 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
 5281 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
 5341 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
 5401 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
 5461 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
 5521 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
 5581 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
 5641 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca
 5701 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
 5761 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
 5821 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
 5881 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
 5941 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacggg
 6001 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
 6061 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
 6121 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
 6181 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
 6241 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
 6301 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
 6361 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
 6421 tcacgaggcc ctttcgtctt caagaattag cttggccatt gcatacgttg tatccatatc
 6481 ataatatgta catttatatt ggctcatgtc caacattacc gccatgttga cattgattat
 6541 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt

//

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