pRS143

LOCUS       pRS143               12434 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  CRISPRi plasmid for use in Candida albicans. Contains dCas9 and 
            NEUT5L integration site and the sgRNA cloning site (SNR52 promoter, 
            PacI sites, sgRNA tail).
ACCESSION   .
VERSION     .
KEYWORDS    pRS143
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12434)
  AUTHORS   Wensing L, Sharma J, Uthayakumar D, Proteau Y, Chavez A, Shapiro RS
  TITLE     A CRISPR Interference Platform for Efficient Genetic Repression in 
            Candida albicans.
  JOURNAL   mSphere. 2019 Feb 13;4(1). pii: 4/1/e00002-19. doi: 
            10.1128/mSphere.00002-19.
  PUBMED    30760609
REFERENCE   2  (bases 1 to 12434)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12434
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..31
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    39..55
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     44..66
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     63..79
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     63..79
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        100..118
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             1152..5255
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the N863A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKARGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5268..5288
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     5293..5309
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     5294..5310
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      6246..6443
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     promoter        complement(7044..7387)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     primer_bind     complement(8350..8369)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8351..8369)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8376..8393)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8376..8392)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8385..8407)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8621..8640)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     misc_RNA        complement(8677..8751)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     10203..10224
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10387..10491
                     /gene="bla"
                     /label=AmpR promoter
     CDS             10492..11352
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10710..10729)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11523..12111
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12012..12031
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12265..12282
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12399..12420
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
       61 cacaggaaac agctatgacc atgattacgc caagctcgaa attaaccctc actaaaggga
      121 acaaaagctg gtaccagagc tattaagatc accagcctcg tttataataa acttagtcta
      181 atatttcttt tggaccaaaa tagaaactgt tctcaggtgg caaaaaacag cttaaagtct
      241 gtgttaaaaa aagatctcgg ctatgccaat caaaaaggag tgacattatt atgtaaaaga
      301 agaaatgttg taattccgcg aaacaacaat tttaatgctt actgtgccgc caccaatatc
      361 ctatggccaa ggggccttta aacatgccac ggaaatatac agtcaaagta aacctactat
      421 gtctttagag ccttcaggat caattgtttt taaatgaatg actgcaaaac tagaaaagta
      481 gcctcttaca taagcaagtc atttgtacac cgagagttta gtaaccagtg aagcaagtat
      541 atctggcaac cctgaaactt atctgtgaat tataaagctc ataagtaaaa agtgtagaaa
      601 agaagataag gcagaccaaa tacaaatttg caacagtaca acgaatattt cagcgtcaaa
      661 actagagaat aataaagaaa acgatctttt caaaaagaaa aaacctttta gttttccttt
      721 gttgttgttg tgggtgtgtg ctatttatat tatatagttt actcataata ccataaaata
      781 ttcggtttga ttaggttatt ttaataagct aatttgtttc taatcgtgta atttatgctg
      841 tgtatattaa gtagtgtgtg cactgcccaa aaatgtttgt tgtttatagt cggttaaaga
      901 gaaaaaagaa aaaaagatcc atacacacac gttaattagt tgttcaacgt aatacactca
      961 tattttgttc ttatttgctt tcggtcgctg ttctcaccaa gatttattgc caacgaaaca
     1021 attttttttt atatattttc agatttttct ttttttcctt tcctttcctt ttctaatttt
     1081 cactcctggt tttctttctt tcttagaaac attatctcga tattaatatt aaaaaaatat
     1141 aatcattcaa aatggataaa aaatactcca taggtttggc cattggaacc aattcagttg
     1201 gatgggctgt aatcactgat gaatataagg ttccttctaa aaagttcaaa gttttaggaa
     1261 atactgatag acactccatt aaaaagaatt taattggtgc cttgttgttt gacagtggtg
     1321 aaacagctga agcaactcgt ttgaaaagaa cagctagaag aagatatacc agaagaaaga
     1381 acagaatttg ttacttgcaa gaaatctttt ctaatgaaat ggcaaaggtt gatgattctt
     1441 tttttcatag attggaagaa agtttcttag ttgaagaaga taagaaacat gaaagacatc
     1501 caatctttgg taatattgta gatgaagtgg cttaccacga aaaatatcct acaatttatc
     1561 atttgagaaa aaaattagtt gactccactg ataaagctga cttaagactt atatatttgg
     1621 cattggctca catgatcaag ttcagaggtc acttcttaat tgaaggtgat ttgaatccag
     1681 ataattccga tgtcgacaag ttgtttattc aattggttca aacttacaac caacttttcg
     1741 aggaaaatcc aatcaatgcc tcaggtgtgg atgctaaagc tattttatct gctagattgt
     1801 caaaaagtag acgtttggaa aatcttattg cccaattgcc aggtgaaaaa aaaaatggat
     1861 tgtttggaaa cttaattgca ttatctttgg gtttgacccc aaatttcaaa tcaaactttg
     1921 atttggccga ggatgctaaa cttcaattgt ctaaggatac atacgatgac gatttagata
     1981 atcttcttgc ccaaattggt gatcaatatg cagatttatt cttggcagct aagaacttga
     2041 gtgatgcaat tttgttaagt gacattttga gagtcaacac agaaattaca aaagctcctt
     2101 tatccgcctc tatgattaaa agatatgatg aacaccatca agatttaacc ttgttgaaag
     2161 ctcttgtacg tcaacaatta ccagaaaagt ataaagaaat attctttgat caatctaaaa
     2221 atggttacgc tggttatatt gatggtggtg ctagtcaaga agaattttat aagtttatca
     2281 agccaattct tgaaaaaatg gatggtactg aggaattgtt ggttaagttg aatcgagagg
     2341 atttacttcg taagcaaaga acatttgata acggttctat ccctcatcaa atccacttag
     2401 gtgagttaca cgctatcttg agacgtcagg aagattttta cccttttttg aaagacaatc
     2461 gtgaaaaaat tgaaaaaatt ttgactttca gaattcctta ttacgtcggg ccattggcta
     2521 gaggtaattc tagatttgca tggatgactc gtaaatctga agagactatc accccatgga
     2581 attttgagga agttgttgat aagggagcct ccgctcaatc attcatcgag agaatgacca
     2641 atttcgacaa aaacttgcca aatgagaagg tgttacctaa acattctttg ttatatgaat
     2701 atttcactgt ttataatgaa ttaacaaaag tcaaatacgt cactgaaggt atgagaaaac
     2761 cagcattctt aagtggtgaa caaaagaaag ctatcgttga cttgttattc aaaactaatc
     2821 gtaaagtcac tgtcaaacaa cttaaagagg actattttaa gaaaatagag tgtttcgatt
     2881 ccgttgaaat cagtggggtt gaggatagat ttaatgctag tttaggaact tatcacgatt
     2941 tgttgaaaat tatcaaagac aaagattttt tggataatga agaaaacgaa gatatcttgg
     3001 aggacattgt attgactttg actttgtttg aggatagaga aatgatcgaa gaacgtttaa
     3061 aaacttacgc tcacttattc gacgataaag tcatgaaaca attaaaaaga agaagatata
     3121 ccggttgggg aagattatct agaaagttga ttaatggtat tagagataag caaagtggta
     3181 aaaccatctt agatttcctt aaaagtgatg gttttgcaaa cagaaacttt atgcagttga
     3241 ttcatgatga tagtttgacc tttaaggaag atattcaaaa agctcaggtt tctggacagg
     3301 gtgattcatt gcatgaacat attgccaact tagcagggtc tccagctatc aaaaagggaa
     3361 ttttacaaac tgttaaagtt gttgatgagt tggtgaaagt tatgggacgt cataagcctg
     3421 aaaatatagt tatagaaatg gctagagaaa atcaaacaac tcagaaagga caaaagaatt
     3481 ctcgtgaaag aatgaaaaga atagaagaag gaattaaaga gttgggttct caaatcttaa
     3541 aagaacaccc agtcgaaaac acccagttac agaacgagaa gctttacttg tactatttgc
     3601 aaaacgggcg tgacatgtat gtagatcaag agttggatat caatagactt tcagattacg
     3661 atgtcgatca catagttcct cagtcatttt taaaagatga ttccattgac aacaaagttt
     3721 taacaagatc tgataaggct agaggtaaat cagacaatgt gccatcagaa gaagtggtta
     3781 agaaaatgaa gaactattgg agacaattgt taaatgcaaa gttgattact cagagaaaat
     3841 ttgacaactt aactaaagct gaaagaggag gattatccga attagataaa gctggattca
     3901 taaaacgtca gttggttgaa acaagacaaa ttactaaaca tgtagcccaa attttagatt
     3961 cccgaatgaa tactaaatat gatgaaaatg acaaattaat cagagaggtc aaagtgatca
     4021 cattaaagtc aaagttggtg tcagatttcc gaaaggattt tcaattctac aaggtgagag
     4081 agatcaacaa ttatcatcat gctcacgacg catatttgaa tgcagtggta ggaactgcct
     4141 taataaagaa gtatcctaag ttagaatccg aattcgtgta tggtgattat aaagtctacg
     4201 atgtcagaaa gatgatagca aaatcagaac aagaaattgg gaaagccacc gcaaagtatt
     4261 tcttttactc caacatcatg aatttcttta agaccgaaat caccttggct aacggggaaa
     4321 tcagaaaacg tcctttgatt gaaacaaatg gagaaaccgg tgaaattgtg tgggataagg
     4381 ggagagattt tgctactgtt agaaaagttt tatcaatgcc tcaagttaat attgtaaaga
     4441 agactgaagt tcaaactggt ggtttctcta aggaatccat tcttccaaaa cgaaactcag
     4501 acaagttgat cgcccgaaaa aaagattggg atcctaagaa atacggaggt tttgattctc
     4561 ctactgtcgc atattctgtc ttggtcgtgg ctaaagttga aaagggtaaa tccaaaaaat
     4621 tgaaatctgt gaaagaattg ttaggtatta ccatcatgga gcgttcatcc tttgagaaaa
     4681 acccaattga ctttctcgaa gcgaagggct ataaagaagt gaagaaagat ttgattatta
     4741 agttgccaaa gtattctctc tttgaacttg aaaatgggag aaagagaatg ttggcttcag
     4801 ctggagaatt gcaaaaagga aacgagttag ctttgccttc aaagtacgtt aatttcttat
     4861 acttagcatc tcattatgaa aagttgaagg ggtcccctga agacaatgag caaaagcaat
     4921 tattcgtaga acaacataaa cactatttgg atgagataat tgaacaaatc tccgagtttt
     4981 ctaaaagagt tattttggct gatgcaaatt tggataaagt tttatctgct tataataagc
     5041 atcgtgacaa accaattcga gaacaagctg aaaatattat ccacttgttc acattgacta
     5101 acttgggagc tccagccgcg tttaagtact ttgacaccac cattgaccgt aagagatata
     5161 cttcaacaaa agaagtgtta gacgccacat tgattcatca gtccataact ggtttatatg
     5221 aaactagaat cgatttgtca caattggggg gtgatagccg agcggatcct aagaaaaaac
     5281 gaaaagtctg actcgaggtc gacggtatcg ataagcttga tatcgaattc ctgcagcccg
     5341 ggggatccga gtgaaattct ggaaatctgg aaatctggtt ttgtattctt gttattcttc
     5401 tttttgttat tacatatata acttgttact tttttaaaaa aatctttgtt tattttataa
     5461 atatataaaa ctaaatttaa gaaaaagaga aaaatgtttt atttgagaga ttgaaatttt
     5521 acttgaattt agcttagctt ttataaagta ttattatgta aaaaaacaaa acaaatatac
     5581 attaaaaagt taagactata aaatagccac ccaaggcatt tctatatctt gttgttgttg
     5641 ttttcatctt ctgtatcaga ggaacttatt ttattatttt cgtcacgggt attttctctt
     5701 gtttgatgat tcatcccatt cattccatca taaaattcta gaaggaccac ctttgattgt
     5761 aaatagtaat aattaccacc cttatttaat tatttattta acttatttat ttatttatta
     5821 tacatatata caaatctaat aaagtgaaaa tctccccctt cacatttata attggccagt
     5881 ttttttcaaa taagcattcc aaccagcatc tctatacctt ttaccttcaa tatctggatc
     5941 tcttccttta ccaaacaatc ctctaccaac aatgataata tcagttccag tgctaacaac
     6001 ttcatcaaca gttctatatt gttgtcctaa tccatcacct ttatcatcta atccaactcc
     6061 aggtgtcata ataagccaat caaatccttc ttcttgtcca cccatatcac gttgggcaat
     6121 aaatccaata acaaattcct tatcggattt agcaatttca acagtttttt gagaatattc
     6181 tccatacgaa ttcgagctcg tttaaacgtc gacactggat ggcggcgtta gtatcgaatc
     6241 gacagcagta tagcgaccag cattcacata cgattgacgc atgatattac tttctgcgca
     6301 cttaacttcg catctgggca gatgatgtcg aggcgaaaaa aaatataaat cacgctaaca
     6361 tttgattaaa atagaacaac tacaatataa aaaaactata caaatgacaa gttcttgaaa
     6421 acaagaatct ttttattgtc agtactgact cgagttatta tggacatggc atagacatat
     6481 acaaagcttg ttcaccatcg gaagcagtac catcgtataa agcagtatcc aaaccacaca
     6541 aagtgaaacc cattcttcta taagcatgaa tagctggagc attaacattg gtaacttcca
     6601 accacaaatg accagcacct ctttctctgg cgaattcagt agccaaaccc atcaaagctc
     6661 taccaacacc atgacctcta tgttctggag caacttcaat atcttcaaca gtcaatcttc
     6721 tgttccaacc agaataagaa acaacaacga aaccagccaa atcaccatca tcaccataag
     6781 caacgaaagt tctagaatct ggatcaccat cttcaccagc atcggattca tcatcggatt
     6841 catcatctgg gaaaacctta gtcaatggtg gatcaactgg aacttctctc aaagtgaaac
     6901 catcaccagt agcagtaact ctaaaaacag tatcggtagt gaaagaacca tccaaagctt
     6961 caatagcttc agcatcacct ggaacagaag ttctgtatct ataagcagta tcatccaaag
     7021 tagtagacat aattgtagga tccggttgtt tatgttcgga tgtgatgtga gaactgtatc
     7081 ctagcaagat tttaaaagga agtatatgaa agaagaacct cagtggcaaa tcctaacctt
     7141 ttatatttct ctacaggggc gcggcgtggg gacaattcaa cgcgtctgtg aggggagcgt
     7201 ttccctgctc gcaggtctgc agcgaggagc cgtaattttt gcttcgcgcc gtgcggccat
     7261 caaaatgtat ggatgcaaat gattatacat ggggatgtat gggctaaatg tacgggcgac
     7321 agtcacatca tgcccctgag ctgcgcacgt caagactgtc aaggagggta ttctgggcct
     7381 ccatgtcgct ggccgggtga cccggcgggg acgaggcaag cttgatatgc taatgatccc
     7441 actgatgata attcagctaa catcaataac cctcttggct cttggttggt ggtggtttct
     7501 ttagctccct gttttaatcc ttcaactact ccattcccag tgacaccatg agcattagta
     7561 atatctgccc aactactaat tttataaact ccaccaatat attgtttctt cacggtatta
     7621 ccaatatcag caaattttct atcttcaaaa atcataaatt gatgtttacg tgaaagttct
     7681 aataatggtt caatagtgga ttcataggaa aaatcattga ttatatcaat atgagtcttg
     7741 attaagcata cataaggacc caatttatca attaattcaa ggaattcctt agtggtatca
     7801 acatcaattg atgcacataa attggttttc ttcagttcca ttaatcgaaa taatcgttgt
     7861 gctactggtg aggcatgagt ttctgctctc tcactatagg tcttagtgtt gactgtcata
     7921 tcttttgttt gctatttcta tttctatttc tttcttcaac tgtaaaaggg gcgatatata
     7981 aaccagtgtg tatggggttg ttgctcagga attgatcaat gtttaaaaga atggttgaaa
     8041 tcatcattga agaacaaaaa aaaagatttt ttctttcatt atgttatctg tataatatgc
     8101 aggaaataag attgcaaaaa aatagccgtt tttttggata ctatcaaaca agaggaatga
     8161 ctaatacaga attagtgtta cgaatcaatg gcactacagc aactttcaac aaaataatct
     8221 acgttcaaaa tataatatat actattaaat aaaatcaaat aataaaataa ttttaaagaa
     8281 agtagtacca gctctttttt ttgtttccgt ttataccatc caaatcaatt cctattagtc
     8341 tccaattcgc cctatagtga gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt
     8401 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     8461 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     8521 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     8581 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     8641 tcctttctcg ccacgttcgc cggcaaaaaa aagcaccacc gactcggtgc cactttttca
     8701 agttgataac ggactagcct tattttaact tgctatttct agctctaaaa ctgaagagcc
     8761 attaatgctc ttcctcgaaa ttaaaaatag tttacgcaag tctcgaactt ccaccacaat
     8821 ttaattgtaa gaacgctgcg aaaacatatt ctccaatcct attgggccga agtttcagct
     8881 atgccaaagg catcaaattt ttttttataa aattttccac ttttccatac cagtgaaatt
     8941 atcacgtgat ggagtgagta tctataatat ttgattgttt ctttgatgag gcaatactcg
     9001 agtatagtta ttgaccaatg acttttaccg gctaaacaag tggtattcaa gcacaattca
     9061 gttttctgga tctctaataa ttgcaattgc aattgcttca catactctgt ttataattca
     9121 gtttctttct cttgatgtta ctgatatatt aagttaataa ataccaataa ctttttttca
     9181 aaaatcttct attttccttt caattgatta tttaaacacg aatcgttaat aagctgtgat
     9241 tgctttttct ctttcttttt cttttctttg ttcaagtttg aaaaaaaaaa aaaaaggttt
     9301 ctcttgttcg atgtagaatt cagtgggtta tagttgtaat gatatatgga ataaaaagaa
     9361 agaaagagaa gagaaagaga agaagagaag aattaagtaa acgtgtatta gaatttaaaa
     9421 attaaaattc atgataatct ttaataataa ttaactaaga atcacaaaat tgaaaatttg
     9481 aaagaaaaaa gatcactcca aggtcgtgtc aagtgactag aaattcttaa ttaaaatagt
     9541 tataacaaaa aaagaaattt gtatataaca attaagtaaa agaaaaaaaa gcaattcgct
     9601 ttgtataata atacccagaa aaatatattc atatattcat aaatcaaaca tgaaatgaag
     9661 aatgctgaat cacttgatag gatttagttc cattatggta ctaagaagta atattacttc
     9721 tatgaaaagg ttgaatccga gatcaaaatt catcttttca tatactctgg agaaaaaaaa
     9781 aaaagtaatt gtagtaagaa tgacaagtat cagttgagaa aaaaagtaac aaagagaaag
     9841 ctcggaggag gctccccaaa ggttttatca ccaatggtgg taccactaac ccaagaacag
     9901 aaaaagcatt gatattttga aaaatacaat actcatcttt taacccaaac cttttcttgt
     9961 cctttaaaac ttttatctca tgtctgtcca aagtcatatt gtcttactac tttctctcct
    10021 tcaacgtcct tccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
    10081 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
    10141 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
    10201 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
    10261 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
    10321 atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga
    10381 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
    10441 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
    10501 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
    10561 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
    10621 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
    10681 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
    10741 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
    10801 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
    10861 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
    10921 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
    10981 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
    11041 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
    11101 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
    11161 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
    11221 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
    11281 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
    11341 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
    11401 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
    11461 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
    11521 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
    11581 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
    11641 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
    11701 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
    11761 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
    11821 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
    11881 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
    11941 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
    12001 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
    12061 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
    12121 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
    12181 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
    12241 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgta agagcgccca
    12301 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
    12361 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
    12421 taggcacccc aggc
//