pGL4.26

价格:1250元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Promega
载体名称: pGL4.26 、pGL4.26[luc2/minP/Hygro]
质粒类型: 哺乳细胞表达;信号通路报告载体
高拷贝/低拷贝: --
启动子: 弱启动子
克隆方法: 多克隆位点,限制性内切酶
载体大小: 5882 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄青霉素Ampicillin
筛选标记: 潮霉素Hygromycin
备注: pGL4.26载体含有荧光素酶报告基因luc2和弱启动子,用于研究细胞内信号通路中转录应答元件的活性。
产品目录号: E8441
稳定性: --
组成型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pGL4.26载体图谱



pGL4.26多克隆位点

pGL4.26载体特征

载体简介

The pGL4.26[luc2/minP/Hygro] Vector(a–e) encodes the luciferase reporter gene luc2 (Photinus pyralis) and is
designed for high expression and reduced anomalous transcription. The vector contains a multiple cloning region for insertion
of a response element of interest upstream of a minimal promoter and the luc2 gene. luc2 is a synthetically-derived
luciferase sequence with humanized codon optimization. The vector backbone contains an ampicillin resistance gene to
allow selection in E. coli and a mammalian selectable marker for hygromycin resistance.

载体序列

LOCUS       pGL4.26[luc2/minP/Hygro]	5882 bp 	DNA	SYN
DEFINITION  pGL4.26[luc2/minP/Hygro]
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..5882
                     /organism="pGL4.26[luc2/minP/Hygro]"
                     /mol_type="other DNA"
     CDS             90..1793
                     /label="ORF frame 3"
                     /translation="MEARLPAWQSGTVGKATMEDAKNIKKGPAPFYPLEDGTAGEQLH
                     KAMKRYALVPGTIAFTDAHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENS
                     LQFFMPVLGALFIGVAVAPANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKL
                     PIIQKIIIMDSKTDYQGFQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGS
                     TGLPKGVALPHRTACVRFSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICG
                     FRVVLMYRFEEELFLRSLQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGA
                     PLSKEVGEAVAKRFHLPGIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVD
                     LDTGKTLGVNQRGELCVRGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFF
                     IVDRLKSLIKYKGYQVAPAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGK
                     TMTEKEIVDYVASQVTTAKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIA
                     V*"
     terminator      complement(1834..1953)
                     /label="SV40_PA_terminator"
                     /translation="MEARLPAWQSGTVGKATMEDAKNIKKGPAPFYPLEDGTAGEQLH
                     KAMKRYALVPGTIAFTDAHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENS
                     LQFFMPVLGALFIGVAVAPANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKL
                     PIIQKIIIMDSKTDYQGFQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGS
                     TGLPKGVALPHRTACVRFSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICG
                     FRVVLMYRFEEELFLRSLQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGA
                     PLSKEVGEAVAKRFHLPGIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVD
                     LDTGKTLGVNQRGELCVRGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFF
                     IVDRLKSLIKYKGYQVAPAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGK
                     TMTEKEIVDYVASQVTTAKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIA
                     V*"
     misc_feature    complement(1848..1867)
                     /label="EBV_rev_primer"
                     /translation="MEARLPAWQSGTVGKATMEDAKNIKKGPAPFYPLEDGTAGEQLH
                     KAMKRYALVPGTIAFTDAHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENS
                     LQFFMPVLGALFIGVAVAPANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKL
                     PIIQKIIIMDSKTDYQGFQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGS
                     TGLPKGVALPHRTACVRFSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICG
                     FRVVLMYRFEEELFLRSLQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGA
                     PLSKEVGEAVAKRFHLPGIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVD
                     LDTGKTLGVNQRGELCVRGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFF
                     IVDRLKSLIKYKGYQVAPAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGK
                     TMTEKEIVDYVASQVTTAKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIA
                     V*"
     misc_feature    complement(2175..2195)
                     /label="pBABE_3_primer"
                     /translation="MEARLPAWQSGTVGKATMEDAKNIKKGPAPFYPLEDGTAGEQLH
                     KAMKRYALVPGTIAFTDAHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENS
                     LQFFMPVLGALFIGVAVAPANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKL
                     PIIQKIIIMDSKTDYQGFQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGS
                     TGLPKGVALPHRTACVRFSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICG
                     FRVVLMYRFEEELFLRSLQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGA
                     PLSKEVGEAVAKRFHLPGIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVD
                     LDTGKTLGVNQRGELCVRGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFF
                     IVDRLKSLIKYKGYQVAPAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGK
                     TMTEKEIVDYVASQVTTAKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIA
                     V*"
     misc_feature    complement(2181..2396)
                     /label="SV40_enhancer"
                     /translation="MEARLPAWQSGTVGKATMEDAKNIKKGPAPFYPLEDGTAGEQLH
                     KAMKRYALVPGTIAFTDAHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENS
                     LQFFMPVLGALFIGVAVAPANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKL
                     PIIQKIIIMDSKTDYQGFQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGS
                     TGLPKGVALPHRTACVRFSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICG
                     FRVVLMYRFEEELFLRSLQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGA
                     PLSKEVGEAVAKRFHLPGIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVD
                     LDTGKTLGVNQRGELCVRGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFF
                     IVDRLKSLIKYKGYQVAPAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGK
                     TMTEKEIVDYVASQVTTAKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIA
                     V*"
     promoter        2193..2461
                     /label="SV40_promoter"
                     /translation="MEARLPAWQSGTVGKATMEDAKNIKKGPAPFYPLEDGTAGEQLH
                     KAMKRYALVPGTIAFTDAHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENS
                     LQFFMPVLGALFIGVAVAPANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKL
                     PIIQKIIIMDSKTDYQGFQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGS
                     TGLPKGVALPHRTACVRFSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICG
                     FRVVLMYRFEEELFLRSLQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGA
                     PLSKEVGEAVAKRFHLPGIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVD
                     LDTGKTLGVNQRGELCVRGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFF
                     IVDRLKSLIKYKGYQVAPAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGK
                     TMTEKEIVDYVASQVTTAKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIA
                     V*"
     rep_origin      2360..2437
                     /label="SV40_origin"
                     /translation="MEARLPAWQSGTVGKATMEDAKNIKKGPAPFYPLEDGTAGEQLH
                     KAMKRYALVPGTIAFTDAHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENS
                     LQFFMPVLGALFIGVAVAPANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKL
                     PIIQKIIIMDSKTDYQGFQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGS
                     TGLPKGVALPHRTACVRFSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICG
                     FRVVLMYRFEEELFLRSLQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGA
                     PLSKEVGEAVAKRFHLPGIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVD
                     LDTGKTLGVNQRGELCVRGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFF
                     IVDRLKSLIKYKGYQVAPAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGK
                     TMTEKEIVDYVASQVTTAKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIA
                     V*"
     misc_feature    2422..2441
                     /label="SV40pro_F_primer"
                     /translation="MEARLPAWQSGTVGKATMEDAKNIKKGPAPFYPLEDGTAGEQLH
                     KAMKRYALVPGTIAFTDAHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENS
                     LQFFMPVLGALFIGVAVAPANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKL
                     PIIQKIIIMDSKTDYQGFQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGS
                     TGLPKGVALPHRTACVRFSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICG
                     FRVVLMYRFEEELFLRSLQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGA
                     PLSKEVGEAVAKRFHLPGIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVD
                     LDTGKTLGVNQRGELCVRGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFF
                     IVDRLKSLIKYKGYQVAPAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGK
                     TMTEKEIVDYVASQVTTAKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIA
                     V*"
     CDS             2540..3577
                     /label="ORF frame 2"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKEVGRV*"
     terminator      3601..3649
                     /label="synth_PA_terminator"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKEVGRV*"
     rep_origin      complement(3961..4580)
                     /label="pBR322_origin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKEVGRV*"
     CDS             complement(4764..5624)
                     /label="ORF frame 1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     terminator      5729..5882
                     /label="up_PA_terminator"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     terminator      5729..5777
                     /label="synth_PA_terminator"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    5729..5882
                     /label="TB"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    5831..5850
                     /label="RVprimer3_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 GGCCTAACTG GCCGGTACCT GAGCTCGCTA GCCTCGAGGA TATCAAGATC TGGCCTCGGC
   61 GGCCAAGCTT AGACACTAGA GGGTATATAA TGGAAGCTCG ACTTCCAGCT TGGCAATCCG
  121 GTACTGTTGG TAAAGCCACC ATGGAAGATG CCAAAAACAT TAAGAAGGGC CCAGCGCCAT
  181 TCTACCCACT CGAAGACGGG ACCGCCGGCG AGCAGCTGCA CAAAGCCATG AAGCGCTACG
  241 CCCTGGTGCC CGGCACCATC GCCTTTACCG ACGCACATAT CGAGGTGGAC ATTACCTACG
  301 CCGAGTACTT CGAGATGAGC GTTCGGCTGG CAGAAGCTAT GAAGCGCTAT GGGCTGAATA
  361 CAAACCATCG GATCGTGGTG TGCAGCGAGA ATAGCTTGCA GTTCTTCATG CCCGTGTTGG
  421 GTGCCCTGTT CATCGGTGTG GCTGTGGCCC CAGCTAACGA CATCTACAAC GAGCGCGAGC
  481 TGCTGAACAG CATGGGCATC AGCCAGCCCA CCGTCGTATT CGTGAGCAAG AAAGGGCTGC
  541 AAAAGATCCT CAACGTGCAA AAGAAGCTAC CGATCATACA AAAGATCATC ATCATGGATA
  601 GCAAGACCGA CTACCAGGGC TTCCAAAGCA TGTACACCTT CGTGACTTCC CATTTGCCAC
  661 CCGGCTTCAA CGAGTACGAC TTCGTGCCCG AGAGCTTCGA CCGGGACAAA ACCATCGCCC
  721 TGATCATGAA CAGTAGTGGC AGTACCGGAT TGCCCAAGGG CGTAGCCCTA CCGCACCGCA
  781 CCGCTTGTGT CCGATTCAGT CATGCCCGCG ACCCCATCTT CGGCAACCAG ATCATCCCCG
  841 ACACCGCTAT CCTCAGCGTG GTGCCATTTC ACCACGGCTT CGGCATGTTC ACCACGCTGG
  901 GCTACTTGAT CTGCGGCTTT CGGGTCGTGC TCATGTACCG CTTCGAGGAG GAGCTATTCT
  961 TGCGCAGCTT GCAAGACTAT AAGATTCAAT CTGCCCTGCT GGTGCCCACA CTATTTAGCT
 1021 TCTTCGCTAA GAGCACTCTC ATCGACAAGT ACGACCTAAG CAACTTGCAC GAGATCGCCA
 1081 GCGGCGGGGC GCCGCTCAGC AAGGAGGTAG GTGAGGCCGT GGCCAAACGC TTCCACCTAC
 1141 CAGGCATCCG CCAGGGCTAC GGCCTGACAG AAACAACCAG CGCCATTCTG ATCACCCCCG
 1201 AAGGGGACGA CAAGCCTGGC GCAGTAGGCA AGGTGGTGCC CTTCTTCGAG GCTAAGGTGG
 1261 TGGACTTGGA CACCGGTAAG ACACTGGGTG TGAACCAGCG CGGCGAGCTG TGCGTCCGTG
 1321 GCCCCATGAT CATGAGCGGC TACGTTAACA ACCCCGAGGC TACAAACGCT CTCATCGACA
 1381 AGGACGGCTG GCTGCACAGC GGCGACATCG CCTACTGGGA CGAGGACGAG CACTTCTTCA
 1441 TCGTGGACCG GCTGAAGAGC CTGATCAAAT ACAAGGGCTA CCAGGTAGCC CCAGCCGAAC
 1501 TGGAGAGCAT CCTGCTGCAA CACCCCAACA TCTTCGACGC CGGGGTCGCC GGCCTGCCCG
 1561 ACGACGATGC CGGCGAGCTG CCCGCCGCAG TCGTCGTGCT GGAACACGGT AAAACCATGA
 1621 CCGAGAAGGA GATCGTGGAC TATGTGGCCA GCCAGGTTAC AACCGCCAAG AAGCTGCGCG
 1681 GTGGTGTTGT GTTCGTGGAC GAGGTGCCTA AAGGACTGAC CGGCAAGTTG GACGCCCGCA
 1741 AGATCCGCGA GATTCTCATT AAGGCCAAGA AGGGCGGCAA GATCGCCGTG TAATAATTCT
 1801 AGAGTCGGGG CGGCCGGCCG CTTCGAGCAG ACATGATAAG ATACATTGAT GAGTTTGGAC
 1861 AAACCACAAC TAGAATGCAG TGAAAAAAAT GCTTTATTTG TGAAATTTGT GATGCTATTG
 1921 CTTTATTTGT AACCATTATA AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT
 1981 TTATGTTTCA GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA
 2041 AATGTGGTAA AATCGATAAG GATCCGTTTG CGTATTGGGC GCTCTTCCGC TGATCTGCGC
 2101 AGCACCATGG CCTGAAATAA CCTCTGAAAG AGGAACTTGG TTAGCTACCT TCTGAGGCGG
 2161 AAAGAACCAG CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC
 2221 AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC
 2281 AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT
 2341 CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC
 2401 CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCTG CCTCTGAGCT
 2461 ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCGATTC
 2521 TTCTGACACT AGCGCCACCA TGAAGAAGCC CGAACTCACC GCTACCAGCG TTGAAAAATT
 2581 TCTCATCGAG AAGTTCGACA GTGTGAGCGA CCTGATGCAG TTGTCGGAGG GCGAAGAGAG
 2641 CCGAGCCTTC AGCTTCGATG TCGGCGGACG CGGCTATGTA CTGCGGGTGA ATAGCTGCGC
 2701 TGATGGCTTC TACAAAGACC GCTACGTGTA CCGCCACTTC GCCAGCGCTG CACTACCCAT
 2761 CCCCGAAGTG TTGGACATCG GCGAGTTCAG CGAGAGCCTG ACATACTGCA TCAGTAGACG
 2821 CGCCCAAGGC GTTACTCTCC AAGACCTCCC CGAAACAGAG CTGCCTGCTG TGTTACAGCC
 2881 TGTCGCCGAA GCTATGGATG CTATTGCCGC CGCCGACCTC AGTCAAACCA GCGGCTTCGG
 2941 CCCATTCGGG CCCCAAGGCA TCGGCCAGTA CACAACCTGG CGGGATTTCA TTTGCGCCAT
 3001 TGCTGATCCC CATGTCTACC ACTGGCAGAC CGTGATGGAC GACACCGTGT CCGCCAGCGT
 3061 AGCTCAAGCC CTGGACGAAC TGATGCTGTG GGCCGAAGAC TGTCCCGAGG TGCGCCACCT
 3121 CGTCCATGCC GACTTCGGCA GCAACAACGT CCTGACCGAC AACGGCCGCA TCACCGCCGT
 3181 AATCGACTGG TCCGAAGCTA TGTTCGGGGA CAGTCAGTAC GAGGTGGCCA ACATCTTCTT
 3241 CTGGCGGCCC TGGCTGGCTT GCATGGAGCA GCAGACTCGC TACTTCGAGC GCCGGCATCC
 3301 CGAGCTGGCC GGCAGCCCTC GTCTGCGAGC CTACATGCTG CGCATCGGCC TGGATCAGCT
 3361 CTACCAGAGC CTCGTGGACG GCAACTTCGA CGATGCTGCC TGGGCTCAAG GCCGCTGCGA
 3421 TGCCATCGTC CGCAGCGGGG CCGGCACCGT CGGTCGCACA CAAATCGCTC GCCGGAGCGC
 3481 AGCCGTATGG ACCGACGGCT GCGTCGAGGT GCTGGCCGAC AGCGGCAACC GCCGGCCCAG
 3541 TACACGACCG CGCGCTAAGG AGGTAGGTCG AGTTTAAACT CTAGAACCGG TCATGGCCGC
 3601 AATAAAATAT CTTTATTTTC ATTACATCTG TGTGTTGGTT TTTTGTGTGT TCGAACTAGA
 3661 TGCTGTCGAC CGATGCCCTT GAGAGCCTTC AACCCAGTCA GCTCCTTCCG GTGGGCGCGG
 3721 GGCATGACTA TCGTCGCCGC ACTTATGACT GTCTTCTTTA TCATGCAACT CGTAGGACAG
 3781 GTGCCGGCAG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT
 3841 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA
 3901 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC
 3961 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG
 4021 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG
 4081 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT
 4141 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT
 4201 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG
 4261 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT
 4321 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT
 4381 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT
 4441 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC
 4501 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC
 4561 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG
 4621 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA
 4681 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGCGGCCG
 4741 CAAATGCTAA ACCACTGCAG TGGTTACCAG TGCTTGATCA GTGAGGCACC GATCTCAGCG
 4801 ATCTGCCTAT TTCGTTCGTC CATAGTGGCC TGACTCCCCG TCGTGTAGAT CACTACGATT
 4861 CGTGAGGGCT TACCATCAGG CCCCAGCGCA GCAATGATGC CGCGAGAGCC GCGTTCACCG
 4921 GCCCCCGATT TGTCAGCAAT GAACCAGCCA GCAGGGAGGG CCGAGCGAAG AAGTGGTCCT
 4981 GCTACTTTGT CCGCCTCCAT CCAGTCTATG AGCTGCTGTC GTGATGCTAG AGTAAGAAGT
 5041 TCGCCAGTGA GTAGTTTCCG AAGAGTTGTG GCCATTGCTA CTGGCATCGT GGTATCACGC
 5101 TCGTCGTTCG GTATGGCTTC GTTCAACTCT GGTTCCCAGC GGTCAAGCCG GGTCACATGA
 5161 TCACCCATAT TATGAAGAAA TGCAGTCAGC TCCTTAGGGC CTCCGATCGT TGTCAGAAGT
 5221 AAGTTGGCCG CGGTGTTGTC GCTCATGGTA ATGGCAGCAC TACACAATTC TCTTACCGTC
 5281 ATGCCATCCG TAAGATGCTT TTCCGTGACC GGCGAGTACT CAACCAAGTC GTTTTGTGAG
 5341 TAGTGTATAC GGCGACCAAG CTGCTCTTGC CCGGCGTCTA TACGGGACAA CACCGCGCCA
 5401 CATAGCAGTA CTTTGAAAGT GCTCATCATC GGGAATCGTT CTTCGGGGCG GAAAGACTCA
 5461 AGGATCTTGC CGCTATTGAG ATCCAGTTCG ATATAGCCCA CTCTTGCACC CAGTTGATCT
 5521 TCAGCATCTT TTACTTTCAC CAGCGTTTCG GGGTGTGCAA AAACAGGCAA GCAAAATGCC
 5581 GCAAAGAAGG GAATGAGTGC GACACGAAAA TGTTGGATGC TCATACTCGT CCTTTTTCAA
 5641 TATTATTGAA GCATTTATCA GGGTTACTAG TACGTCTCTC AAGGATAAGT AAGTAATATT
 5701 AAGGTACGGG AGGTATTGGA CAGGCCGCAA TAAAATATCT TTATTTTCAT TACATCTGTG
 5761 TGTTGGTTTT TTGTGTGAAT CGATAGTACT AACATACGCT CTCCATCAAA ACAAAACGAA
 5821 ACAAAACAAA CTAGCAAAAT AGGCTGTCCC CAGTGCAAGT GCAGGTGCCA GAACATTTCT
 5881 CT
//

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