pET-38b(+)

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pET38b载体基本信息

出品公司: Novagen
载体名称: pET-38b(+), pET38b载体,pET38b质粒,pET-38b载体,pET-38b质粒
质粒类型: 大肠杆菌表达载体
高拷贝/低拷贝: 高拷贝
克隆方法: 多克隆位点,限制性内切酶
载体大小: 5821 bp 
5' 测序引物及序列: T7
3' 测序引物及序列: 5'-TAATACGACTCACTATAGGG-3'
载体标签: His,C-CBD
载体抗性: 卡那霉素
表达宿主菌: BL21(DE3)BLR(DE3)Rosetta(DE3)OrigamiB(DE3)
pET表达手册: pET表达手册英文版
pET表达手册中文版
pET载体汇总:
pET-3a     pET-11a   pET-12a   pET-14b   pET-15b   pET-16b
pET-17b   pET-19b   pET-20b   pET-21a   pET-22b   pET-23a
pET-24a   pET-25b   pET-26b   pET-27b   pET-28a   pET-29a
pET-30a   pET-31b   pET-32a   pET-33b   pET-34b   pET-35b
pET-37b   pET-39b   pET-40b   pET-41a   pET-42a   pET-43.1a
pET-44a   pET-45b   pET-47b   pET-48b   pET-49b   pET-50b
pET-51b   pET-52b  
pETDuet-1  pACYCDuet-1  pCDFDuet-1 pRSFDuet-1  pColaDuet-1

pET38b载体图谱质粒图谱和多克隆位点信息

pET-38b(+)载体图谱
pET-38b(+)-1载体图谱

pET38b载体简介

pET-38b(+)向量(Cat. No. 70137-3)用于表达C-终端CBDCEX 融合蛋白。插入N端信号序列和C端之间的目标序列CBDcex•Tag和His•Tag的阅读框(如下所示)中阅读,以产生所需的多肽 引信。有多种克隆地点和策略可供选择。独特的地点显示在圆圈上 地图。注意,序列由pBR322约定编号,因此T7表达式区域 在圆形地图上是相反的。T7转录的编码链的克隆/表达区 rna聚合酶如下所示。f1基因的起源是定向的,因此辅助噬菌体的感染将 产生含有与编码链相对应的单链DNA的病毒离子。因此, 应使用T7终止引物(Cat. No. 69337-3)。



pET Vectors 选择指南

pET38b载体序列

LOCUS       pET-38b              5821 bp ds-DNA     circular SYN 08-MAY-2022
DEFINITION  synthetic circular DNA.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5821)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sunday, May 8, 2022 from SnapGene Viewer 5.1.4.1 to Vector 
            NTI(R) format
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     VNTDATE|984960000|
COMMENT     VNTDBDATE|984960000|
COMMENT     VNTNAME|pET-38b---|
FEATURES             Location/Qualifiers
     source          1..5821
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=source
     terminator      26..73
                     /label=terminator
                     /note="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(150..173)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis affinity tag
                     /note="8xHis"
     CDS             complement(528..545)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin recognition and cleavage site
                     /note="thrombin site"
     RBS             754..776
                     /label=ribosome binding site
                     /note="efficient ribosome binding site from bacteriophageT7
                     gene 10 (Olins and Rangwala, 1989)"
     protein_bind    791..815
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator 
                     toinhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(816..834)
                     /label=promoter
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        1147..1224
                     /gene="lacI"
                     /label=lacI promoter
                     /note="lacI promoter"
     CDS             1225..2307
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="lacI"
                     /note="The lac repressor binds to the lac operator 
                     toinhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     CDS             3116..3307
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low 
                     copynumber"
                     /label=rop
                     /note="rop"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     misc_feature    3409..3551
                     /label=bom
                     /note="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(3737..4325)
                     /direction=LEFT
                     /label=ori
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             4447..5262
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=aph(3')-Ia
                     /note="KanR"
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
     rep_origin      complement(5355..5810)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; 
                     arrowindicates direction of (+) strand synthesis"
ORIGIN
        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
      121 tgtttagcag cctaggtatt aatcaattag tggtggtggt ggtggtggtg gtgctcgacg
      181 ccgaccgtgc agggcgtgcc gttgagcgag aacgccgtcg gcgcggcgtt ggtgcccgtg
      241 tgcgagccgt tgaagccgaa ctgcgcggtg ccgcccgccg ggatcgagcc gttccacggg
      301 gcgttgcgga ccgtcacggc cgagccggac tgcgtgaccg tcgagctcca cgcctgggtg
      361 acctgctggc cggacgggaa gctgaacgtg agcgtccagc cgtctaccgg agcggaggac
      421 gtgttcttca cggtgacgtt cgcggtgaag ccggtgttcc actggttgac gccccacagc
      481 acctggcacc cggccggacc actagtcggg gtcggcgtcg gaggaccgga accgcgtggc
      541 accagaccct cgagtgcggc cgcaagcttg acgtcctgca ggtgtacaga attcggatcc
      601 gatatcacca tggtgctagc tgcctgagct ggcagaacaa cagttgcacc tacaaccagg
      661 gtagcagcag cagcagccag agtagtacgc agagcagtac gagcaccacg agccggatga
      721 cctggagctg gagtagtacg aggcatatgt atatctcctt cttaaagtta aacaaaatta
      781 tttctagagg ggaattgtta tccgctcaca attcccctat agtgagtcgt attaatttcg
      841 cgggatcgag atcgatctcg atcctctacg ccggacgcat cgtggccggc atcaccggcg
      901 ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc
      961 gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg
     1021 ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg gtgctcaacg
     1081 gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgtcgag
     1141 atcccggaca ccatcgaatg gcgcaaaacc tttcgcggta tggcatgata gcgcccggaa
     1201 gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat
     1261 gccggtgtct cttatcagac cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg
     1321 aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg
     1381 gcacaacaac tggcgggcaa acagtcgttg ctgattggcg ttgccacctc cagtctggcc
     1441 ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca actgggtgcc
     1501 agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac
     1561 aatcttctcg cgcaacgcgt cagtgggctg atcattaact atccgctgga tgaccaggat
     1621 gccattgctg tggaagctgc ctgcactaat gttccggcgt tatttcttga tgtctctgac
     1681 cagacaccca tcaacagtat tattttctcc catgaagacg gtacgcgact gggcgtggag
     1741 catctggtcg cattgggtca ccagcaaatc gcgctgttag cgggcccatt aagttctgtc
     1801 tcggcgcgtc tgcgtctggc tggctggcat aaatatctca ctcgcaatca aattcagccg
     1861 atagcggaac gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg
     1921 ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca acgatcagat ggcgctgggc
     1981 gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg cggacatctc ggtagtggga
     2041 tacgacgata ccgaagacag ctcatgttat atcccgccgt taaccaccat caaacaggat
     2101 tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg
     2161 gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc
     2221 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     2281 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtaagtt agctcactca
     2341 ttaggcaccg ggatctcgac cgatgccctt gagagccttc aacccagtca gctccttccg
     2401 gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact
     2461 cgtaggacag gtgccggcag cgctctgggt cattttcggc gaggaccgct ttcgctggag
     2521 cgcgacgatg atcggcctgt cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc
     2581 cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag caggccatta tcgccggcat
     2641 ggcggcccca cgggtgcgca tgatcgtgct cctgtcgttg aggacccggc taggctggcg
     2701 gggttgcctt actggttagc agaatgaatc accgatacgc gagcgaacgt gaagcgactg
     2761 ctgctgcaaa acgtctgcga cctgagcaac aacatgaatg gtcttcggtt tccgtgtttc
     2821 gtaaagtctg gaaacgcgga agtcagcgcc ctgcaccatt atgttccgga tctgcatcgc
     2881 aggatgctgc tggctaccct gtggaacacc tacatctgta ttaacgaagc gctggcattg
     2941 accctgagtg atttttctct ggtcccgccg catccatacc gccagttgtt taccctcaca
     3001 acgttccagt aaccgggcat gttcatcatc agtaacccgt atcgtgagca tcctctctcg
     3061 tttcatcggt atcattaccc ccatgaacag aaatccccct tacacggagg catcagtgac
     3121 caaacaggaa aaaaccgccc ttaacatggc ccgctttatc agaagccaga cattaacgct
     3181 tctggagaaa ctcaacgagc tggacgcgga tgaacaggca gacatctgtg aatcgcttca
     3241 cgaccacgct gatgagcttt accgcagctg cctcgcgcgt ttcggtgatg acggtgaaaa
     3301 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     3361 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac
     3421 ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt
     3481 gtactgagag tgcaccatat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     3541 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     3601 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     3661 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     3721 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     3781 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     3841 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     3901 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     3961 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     4021 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     4081 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     4141 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     4201 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     4261 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     4321 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     4381 gttaagggat tttggtcatg aacaataaaa ctgtctgctt acataaacag taatacaagg
     4441 ggtgttatga gccatattca acgggaaacg tcttgctcta ggccgcgatt aaattccaac
     4501 atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg
     4561 acaatctatc gattgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa
     4621 ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt
     4681 atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc
     4741 actgcgatcc ccgggaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa
     4801 aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat
     4861 tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac
     4921 ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc
     4981 tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac tcatggtgat
     5041 ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga
     5101 cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag
     5161 ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg
     5221 aataaattgc agtttcattt gatgctcgat gagtttttct aagaattaat tcatgagcgg
     5281 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     5341 aaaagtgcca cctgaaattg taaacgttaa tattttgtta aaattcgcgt taaatttttg
     5401 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
     5461 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     5521 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     5581 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     5641 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     5701 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
     5761 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc
     5821 a
//

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