| 质粒名称: | pBHt2 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 104175 |
| 存储实验室: | Seogchan Kang |
|
载体骨架基本信息 |
|
| 载体名称: | pCAMBIA1300 |
| 载体出品公司: | CAMBIA, Canberra, Australia |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | 在花椰菜花叶病毒35S（CaMV35S）的控制下，用XhoI和bstsxia消化pCAMBIA1300，去除hph基因。将末端钝化并连接到1.4kb的pCB1004 HpaI片段上，该片段在巢曲霉trpC启动子下含有hph基因。然后将hph基因的3′端连接到CaMV35S poly（A）终止信号。 |
| 载体类型: | 真菌表达 |
| 筛选标记: | 潮霉素 |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pBHt2 was a gift from Seogchan Kang (Addgene plasmid # 104175 ; http://n2t.net/addgene:104175 ; RRID:Addgene_104175) |
| 参考文献部分: | Agrobacterium-Mediated Transformation of Fusarium oxysporum: An Efficient Tool for Insertional Mutagenesis and Gene Transfer. Mullins ED, Chen X, Romaine P, Raina R, Geiser DM, Kang S. Phytopathology. 2001 Feb;91(2):173-80. doi: 10.1094/PHYTO.2001.91.2.173. 10.1094/PHYTO.2001.91.2.173 PubMed 18944391 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pBHt2 8494 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Empty backbone for Agrobacterium tumefaciens-mediated transformation
of Fusarium oxysporum.
ACCESSION .
VERSION .
KEYWORDS pBHt2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8494)
AUTHORS Mullins ED, Chen X, Romaine P, Raina R, Geiser DM, Kang S
TITLE Agrobacterium-Mediated Transformation of Fusarium oxysporum: An
Efficient Tool for Insertional Mutagenesis and Gene Transfer.
JOURNAL Phytopathology. 2001 Feb;91(2):173-80. doi:
10.1094/PHYTO.2001.91.2.173.
PUBMED 18944391
REFERENCE 2 (bases 1 to 8494)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8494
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 1..25
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
polyA_signal 103..277
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
CDS complement(308..1333)
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANILFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
E"
protein_bind 1813..1834
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 1849..1879
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 1887..1903
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 1892..1914
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 1911..1927
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 1911..1927
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
misc_feature 1936..1992
/label=MCS
/note="pUC18/19 multiple cloning site"
primer_bind complement(1996..2013)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(1996..2012)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2005..2027)
/label=M13/pUC Forward
/note="In lacZ gene"
misc_feature 2215..2239
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
CDS 3540..4169
/codon_start=1
/product="stability protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 StaA
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
CDS 4598..5671
/codon_start=1
/product="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 RepA
/translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
LVMRYRNLIEGEASAGS"
rep_origin 5737..5931
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
primer_bind complement(6167..6189)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
misc_feature 6275..6415
/label=bom
/note="basis of mobility region from pBR322"
primer_bind 6330..6349
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind complement(6430..6447)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6601..7189)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6681..6700)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7276..8070)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
61 gacgttttta atgtactgaa ttaacgccga attaattcgg gggatctgga ttttagtact
121 ggattttggt tttaggaatt agaaatttta ttgatagaag tattttacaa atacaaatac
181 atactaaggg tttcttatat gctcaacaca tgagcgaaac cctataggaa ccctaattcc
241 cttatctggg aactactcac acattattat ggagaaactc gaaactggtt cccggtcggc
301 atctactcta ttcctttgcc ctcggacgag tgctggggcg tcggtttcca ctatcggcga
361 gtacttctac acagccatcg gtccagacgg ccgcgcttct gcgggcgatt tgtgtacgcc
421 cgacagtccc ggctccggat cggacgattg cgtcgcatcg accctgcgcc caagctgcat
481 catcgaaatt gccgtcaacc aagctctgat agagttggtc aagaccaatg cggagcatat
541 acgcccggag gcgcggcgat cctgcaagct ccggatgcct ccgctcgaag tagcgcgtct
601 gctgctccat acaagccaac cacggcctcc agaagaggat gttggcgacc tcgtattggg
661 aatccccgaa catcgcctcg ctccagtcaa tgaccgctgt tatgcggcca ttgtccgtca
721 ggacattgtt ggagccgaaa tccgcatgca cgaggtgccg gacttcgggg cagtcctcgg
781 cccaaagcat cagctcatcg agagcctgcg cgacggacgc actgacggtg tcgtccatca
841 cagtttgcca gtgatacaca tggggatcag caatcgcgca tatgaaatca cgccatgtag
901 tgtattgacc gattccttgc ggtccgaatg ggccgaaccc gctcgtctgg ctaagatcgg
961 ccgcagcgat cgcatccatg gcctccgcga ccggctggag aacagcgggc agttcggttt
1021 caggcaggtc ttgcaacgtg acaccctgtg cacggcggga gatgcaatag gtcaggctct
1081 cgctgaactc cccaatgtca agcacttccg gaatcgggag cgcggccgat gcaaagtgcc
1141 gataaacata acgatctttg tagaaaccat cggcgcagct atttacccgc aggacatatc
1201 cacgccctcc tacatcgaag ctgaaagcac gagattcttc gccctccgag agctgcatca
1261 ggtcggagac gctgtcgaac ttttcgatca gaaacttctc gacagacgtc gcggtgagtt
1321 caggcttttt catttggatg cttgggtaga ataggtaagt cagattgaat ctgaaataaa
1381 gggaggaagg gcgaacttaa gaaggtatga ccgggtcgtc cacttacctt gcttgacaaa
1441 cgcaccaagt tatcgtgcac caagcagcag atgataataa tgtcctcgtt cctgtctgct
1501 aataagagtc acacttcgag cgccgccgct actgctacaa gtggggctga tctgaccagt
1561 tgcctaaatg aaccatcttg tcaaacgaca caaattttgt gctcaccgcc tggacgacta
1621 aaccaaaata ggcattcatt gttgacctcc actagctcca gccaagccca aaaaatgctc
1681 cttcaatatc agttttggca agctgctcta gccaatacgc aaaccgcctc tccccgcgcg
1741 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
1801 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
1861 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
1921 ctatgacatg attacgaatt cgagctcggt acccggggat cctctagagt cgacctgcag
1981 gcatgcaagc ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
2041 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
2101 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gctagagcag
2161 cttgagcttg gatcagattg tcgtttcccg ccttcagttt aaactatcag tgtttgacag
2221 gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataatc ggatatttaa
2281 aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac cacagggttc
2341 ccctcgggat caaagtactt tgatccaacc cctccgctgc tatagtgcag tcggcttctg
2401 acgttcagtg cagccgtctt ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca
2461 ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc gcataaagta
2521 gaatacttgc gactagaacc ggagacatta cgccatgaac aagagcgccg ccgctggcct
2581 gctgggctat gcccgcgtca gcaccgacga ccaggacttg accaaccaac gggccgaact
2641 gcacgcggcc ggctgcacca agctgttttc cgagaagatc accggcacca ggcgcgaccg
2701 cccggagctg gccaggatgc ttgaccacct acgccctggc gacgttgtga cagtgaccag
2761 gctagaccgc ctggcccgca gcacccgcga cctactggac attgccgagc gcatccagga
2821 ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc gacaccacca cgccggccgg
2881 ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc taatcatcga
2941 ccgcacccgg agcgggcgcg aggccgccaa ggcccgaggc gtgaagtttg gcccccgccc
3001 taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac
3061 cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg accctgtacc gcgcacttga
3121 gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc
3181 attgaccgag gccgacgccc tggcggccgc cgagaatgaa cgccaagagg aacaagcatg
3241 aaaccgcacc aggacggcca ggacgaaccg tttttcatta ccgaagagat cgaggcggag
3301 atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg tctcaaccgt gcggctgcat
3361 gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct ggccggccag cttggccgct
3421 gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt
3481 catgcggtcg ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa ggggaacgca
3541 tgaaggttat cgctgtactt aaccagaaag gcgggtcagg caagacgacc atcgcaaccc
3601 atctagcccg cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat tccgatcccc
3661 agggcagtgc ccgcgattgg gcggccgtgc gggaagatca accgctaacc gttgtcggca
3721 tcgaccgccc gacgattgac cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga
3781 tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg
3841 tgctgattcc ggtgcagcca agcccttacg acatatgggc caccgccgac ctggtggagc
3901 tggttaagca gcgcattgag gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc
3961 gggcgatcaa aggcacgcgc atcggcggtg aggttgccga ggcgctggcc gggtacgagc
4021 tgcccattct tgagtcccgt atcacgcagc gcgtgagcta cccaggcact gccgccgccg
4081 gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg
4141 ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag
4201 cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg caacgttggc
4261 cagcctggca gacacgccag ccatgaagcg ggtcaacttt cagttgccgg cggaggatca
4321 caccaagctg aagatgtacg cggtacgcca aggcaagacc attaccgagc tgctatctga
4381 atacatcgcg cagctaccag agtaaatgag caaatgaata aatgagtaga tgaattttag
4441 cggctaaagg aggcggcatg gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc
4501 ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg ctgggttgcc tgccggccct
4561 gcaatggcac tggaaccccc aagcccgagg aatcggcgtg agcggtcgca aaccatccgg
4621 cccggtacaa atcggcgcgg cgctgggtga tgacctggtg gagaagttga aggccgcgca
4681 ggccgcccag cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc
4741 cgctgatcga atccgcaaag aatcccggca accgccggca gccggtgcgc cgtcgattag
4801 gaagccgccc aagggcgacg agcaaccaga ttttttcgtt ccgatgctct atgacgtggg
4861 cacccgcgat agtcgcagca tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg
4921 acgagctggc gaggtgatcc gctacgagct tccagacggg cacgtagagg tttccgcagg
4981 gccggccggc atggccagtg tgtgggatta cgacctggta ctgatggcgg tttcccatct
5041 aaccgaatcc atgaaccgat accgggaagg gaagggagac aagcccggcc gcgtgttccg
5101 tccacacgtt gcggacgtac tcaagttctg ccggcgagcc gatggcggaa agcagaaaga
5161 cgacctggta gaaacctgca ttcggttaaa caccacgcac gttgccatgc agcgtacgaa
5221 gaaggccaag aacggccgcc tggtgacggt atccgagggt gaagccttga ttagccgcta
5281 caagatcgta aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc tagctgattg
5341 gatgtaccgc gagatcacag aaggcaagaa cccggacgtg ctgacggttc accccgatta
5401 ctttttgatc gatcccggca tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg
5461 caaggcagaa gccagatggt tgttcaagac gatctacgaa cgcagtggca gcgccggaga
5521 gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta
5581 cgatttgaag gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct accgcaacct
5641 gatcgagggc gaagcatccg ccggttccta atgtacggag cagatgctag ggcaaattgc
5701 cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg gatagcacgt acattgggaa
5761 cccaaagccg tacattggga accggaaccc gtacattggg aacccaaagc cgtacattgg
5821 gaaccggtca cacatgtaag tgactgatat aaaagagaaa aaaggcgatt tttccgccta
5881 aaactcttta aaacttatta aaactcttaa aacccgcctg gcctgtgcat aactgtctgg
5941 ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg
6001 ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg
6061 gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc
6121 acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
6181 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
6241 agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg
6301 atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
6361 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc
6421 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
6481 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
6541 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
6601 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
6661 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
6721 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
6781 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
6841 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
6901 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
6961 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
7021 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
7081 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
7141 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
7201 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
7261 catgcattct aggtactaaa acaattcatc cagtaaaata taatatttta ttttctccca
7321 atcaggcttg atccccagta agtcaaaaaa tagctcgaca tactgttctt ccccgatatc
7381 ctccctgatc gaccggacgc agaaggcaat gtcataccac ttgtccgccc tgccgcttct
7441 cccaagatca ataaagccac ttactttgcc atctttcaca aagatgttgc tgtctcccag
7501 gtcgccgtgg gaaaagacaa gttcctcttc gggcttttcc gtctttaaaa aatcatacag
7561 ctcgcgcgga tctttaaatg gagtgtcttc ttcccagttt tcgcaatcca catcggccag
7621 atcgttattc agtaagtaat ccaattcggc taagcggctg tctaagctat tcgtataggg
7681 acaatccgat atgtcgatgg agtgaaagag cctgatgcac tccgcataca gctcgataat
7741 cttttcaggg ctttgttcat cttcatactc ttccgagcaa aggacgccat cggcctcact
7801 catgagcaga ttgctccagc catcatgccg ttcaaagtgc aggacctttg gaacaggcag
7861 ctttccttcc agccatagca tcatgtcctt ttcccgttcc acatcatagg tggtcccttt
7921 ataccggctg tccgtcattt ttaaatatag gttttcattt tctcccacca gcttatatac
7981 cttagcagga gacattcctt ccgtatcttt tacgcagcgg tatttttcga tcagtttttt
8041 caattccggt gatattctca ttttagccat ttattatttc cttcctcttt tctacagtat
8101 ttaaagatac cccaagaagc taattataac aagacgaact ccaattcact gttccttgca
8161 ttctaaaacc ttaaatacca gaaaacagct ttttcaaagt tgttttcaaa gttggcgtat
8221 aacatagtat cgacggagcc gattttgaaa ccgcggtgat cacaggcagc aacgctctgt
8281 catcgttaca atcaacatgc taccctccgc gagatcatcc gtgtttcaaa cccggcagct
8341 tagttgccgt tcttccgaat agcatcggta acatgagcaa agtctgccgc cttacaacgg
8401 ctctcccgct gacgccgtcc cggactgatg ggctgcctgt atcgagtggt gattttgtgc
8461 cgagctgccg gtcggggagc tgttggctgg ctgg
//
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载体质粒应用举例
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