pLNCX2

价格:1600元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Clontech
载体名称: pLNCX2
质粒类型: 逆病毒载体;哺乳动物细胞表达载体
高拷贝/低拷贝: 低拷贝
启动子: CMV
克隆方法: 多克隆位点,限制性内切酶
载体大小: 6132bp
5' 测序引物及序列: pLNCX-F: AGCTGGTTTAGTGAACCGTCAGATC
3' 测序引物及序列: pLNCX-R: ACCTACAGGTGGGGTCTTTCATTCCC
载体标签: --
载体抗性: 氨苄
筛选标记: Neomycin
备注: --
产品目录号: 631503/ 631508/ 631511
稳定性: 稳表达
组成型: 组成型
病毒/非病毒: 逆病毒

载体质粒图谱和多克隆位点信息

pLNCX2载体图谱



pLNCX2多克隆位点

RNAi-Ready pSIREN-RetroQ载体特征

载体简介

载体序列

LOCUS       pLNCX2   6132 bp    DNA   circular             4-DEC-2009
COMMENT     Created by Clontech Laboratories Inc.
COMMENT     Cat. No. 631503,631511,631508,631459
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     LTR             1..589
                     /label=5'\MMSV\LTR
     LTR             3034..3627
                     /label=3'\MMLV\LTR
     misc_signal     659..1468
                     /label=Extended\packaging\signal
     CDS             1512..2306
                     /label=Neo(R)
     promoter        2373..2905
                     /label=CMV\IE\Promoter
     misc_feature    2925..2995
                     /label=MCS
     rep_origin      4163..4163
                     /label=pUC\origin
     CDS             complement(4926..5783)
                     /label=Amp(R)
BASE COUNT     1419 a      1665 c      1575 g      1473 t 
ORIGIN
        1 tttgaaagac cccacccgta ggtggcaagc tagcttaagt aacgccactt tgcaaggcat 
       61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca aagaaacagc 
      121 tgaataccaa acaggatatc tgtggtaagc ggttcctgcc ccggctcagg gccaagaaca 
      181 gatgagacag ctgagtgatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg 
      241 ctcggggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagtgaa 
      301 tcatcagatg tttccagggt gccccaagga cctgaaaatg accctgtacc ttatttgaac 
      361 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc cgctctccga gctcaataaa 
      421 agagcccaca acccctcact cggcgcgcca gtcttccgat agactgcgtc gcccgggtac 
      481 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggtctcg ctgttccttg 
      541 ggagggtctc ctctgagtga ttgactaccc acgacggggg tctttcattt gggggctcgt 
      601 ccgggatttg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc 
      661 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg 
      721 tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt 
      781 ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg 
      841 cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt 
      901 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa 
      961 ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct 
     1021 gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt 
     1081 gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa 
     1141 gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc 
     1201 gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc 
     1261 tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt 
     1321 tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc 
     1381 atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta 
     1441 tccagccctc actccttctc taggcgccgg aattccgatc tgatcaagag acaggatgag 
     1501 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg 
     1561 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt 
     1621 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc 
     1681 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt 
     1741 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag 
     1801 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg 
     1861 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag 
     1921 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg 
     1981 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc 
     2041 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca 
     2101 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc 
     2161 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg 
     2221 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct 
     2281 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaatagt aatcaattac 
     2341 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg 
     2401 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc 
     2461 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac 
     2521 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa 
     2581 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac 
     2641 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta 
     2701 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga 
     2761 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa 
     2821 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag 
     2881 agctggttta gtgaaccgtc agatccgcta gcgctaccgg actcagatct cgagctcaag 
     2941 cttgtttggc cgaggcggcc gcttgtcgac aggccttaat ggcctaacat cgataaaata 
     3001 aaagatttta tttagtctcc agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg 
     3061 caagctagct taagtaacgc cattttgcaa ggcatggaaa aatacataac tgagaataga 
     3121 gaagttcaga tcaaggtcag gaacagatgg aacagctgaa tatgggccaa acaggatatc 
     3181 tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggaacag ctgaatatgg 
     3241 gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg 
     3301 tccccagatg cggtccagcc ctcagcagtt tctagagaac catcagatgt ttccagggtg 
     3361 ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt tcgcttctcg 
     3421 cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac ccctcactcg 
     3481 gggcgccagt cctccgattg actgagtcgc ccgggtaccc gtgtatccaa taaaccctct 
     3541 tgcagttgca tccgacttgt ggtctcgctg ttccttggga gggtctcctc tgagtgattg 
     3601 actacccgtc agcgggggtc tttcatttgg gggctcgtcc gggatcggga gacccctgcc 
     3661 cagggaccac cgacccacca ccgggaggta agctggctgc ctcgcgcgtt tcggtgatga 
     3721 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga 
     3781 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc 
     3841 agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca 
     3901 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg 
     3961 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc 
     4021 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 
     4081 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 
     4141 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 
     4201 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 
     4261 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 
     4321 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 
     4381 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 
     4441 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 
     4501 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 
     4561 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc 
     4621 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 
     4681 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 
     4741 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 
     4801 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 
     4861 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 
     4921 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 
     4981 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 
     5041 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 
     5101 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 
     5161 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 
     5221 aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 
     5281 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 
     5341 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 
     5401 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 
     5461 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 
     5521 tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg 
     5581 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 
     5641 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 
     5701 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 
     5761 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 
     5821 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 
     5881 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg 
     5941 acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga attcatacca 
     6001 gatcaccgaa aactgtcctc caaatgtgtc cccctcacac tcccaaattc gcgggcttct 
     6061 gcctcttaga ccactctacc ctattcccca cactcaccgg agccaaagcc gcggcccttc 
     6121 cgtttctttg ct 
//

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