pBT-4

价格:2500元

联系方式:I47-825O-882O

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载体基本信息

出品人: Lynch,M.D. and Gill,R.T.
载体名称: pBT-4
质粒类型: 广宿主载体;克隆载体;原核载体
高拷贝/低拷贝: --
克隆方法: 限制性内切酶,多克隆位点
启动子:
载体大小: 2936 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: --
载体抗性: 四环素
克隆菌株: DH5α 等
宿主细胞(系): 多个菌属
备注: --
产品目录号: --
组成型/诱导型: 组成型

载体质粒图谱和多克隆位点信息

pBT-4载体图谱



载体简介

载体序列

LOCUS       DQ058722                2936 bp    DNA     circular SYN 08-MAY-2006
DEFINITION  Broad host range vector pBT-4, complete sequence.
ACCESSION   DQ058722
VERSION     DQ058722.1  GI:69048132
KEYWORDS    .
SOURCE      Broad host range vector pBT-4
  ORGANISM  Broad host range vector pBT-4
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Lynch,M.D. and Gill,R.T.
  TITLE     Broad host range vectors for stable genomic library construction
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Lynch,M.D. and Gill,R.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAY-2005) Chemical & Biological Engineering,
            University of Colorado, ECCH 111, Campus Box 424, Boulder, CO
            80309, USA
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Broad host range vector pBT-4"
                     /mol_type="other DNA"
                     /db_xref="taxon:334954"
     regulatory      60..96
                     /regulatory_class="terminator"
                     /note="SoxR terminator"
     gene            872..1534
                     /gene="rep"
                     /note="rep gene from pBBR1, GenBank Accession Number
                     X66730"
     CDS             872..1534
                     /gene="rep"
                     /note="required for replication"
                     /codon_start=1
                     /transl_table=11
                     /product="Rep"
                     /protein_id="AAY99693.1"
                     /db_xref="GI:69048133"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLL
                     HHLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAY
                     VVNDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGP
                     GEEPPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLP
                     RR"
     gene            1631..2821
                     /gene="tetA"
                     /note="tetracycline resistance gene from pBR322"
     CDS             1631..2821
                     /gene="tetA"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline/H+ antiporter"
                     /protein_id="AAY99694.1"
                     /db_xref="GI:69048134"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH
                     YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG
                     RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH
                     APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV
                     FFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGE
                     KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG
                     QLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR
                     ATST"
     regulatory      2841..2872
                     /regulatory_class="terminator"
                     /note="TonB terminator"
ORIGIN      
        1 aacgaattca agcttgatat cattcaggac gagcctcaga ctccagcgta actggactga
       61 aaacaaacta aagcgccctt gtggcgcttt agttttgttc cgcggccacc ggctggctcg
      121 cttcgctcgg cccgtggaca accctgctgg acaagctgat ggacaggctg cgcctgccca
      181 cgagcttgac cacagggatt gcccaccggc tacccagcct tcgaccacat acccaccggc
      241 tccaactgcg cggcctgcgg ccttgcccca tcaatttttt taattttctc tggggaaaag
      301 cctccggcct gcggcctgcg cgcttcgctt gccggttgga caccaagtgg aaggcgggtc
      361 aaggctcgcg cagcgaccgc gcagcggctt ggccttgacg cgcctggaac gacccaagcc
      421 tatgcgagtg ggggcagtcg aaggcgaagc ccgcccgcct gccccccgag cctcacggcg
      481 gcgagtgcgg gggttccaag ggggcagcgc caccttgggc aaggccgaag gccgcgcagt
      541 cgatcaacaa gccccggagg ggccactttt tgccggaggg ggagccgcgc cgaaggcgtg
      601 ggggaacccc gcaggggtgc ccttctttgg gcaccaaaga actagatata gggcgaaatg
      661 cgaaagactt aaaaatcaac aacttaaaaa aggggggtac gcaacagctc attgcggcac
      721 cccccgcaat agctcattgc gtaggttaaa gaaaatctgt aattgactgc cacttttacg
      781 caacgcataa ttgttgtcgc gctgccgaaa agttgcagct gattgcgcat ggtgccgcaa
      841 ccgtgcggca ccctaccgca tggagataag catggccacg cagtccagag aaatcggcat
      901 tcaagccaag aacaagcccg gtcactgggt gcaaacggaa cgcaaagcgc atgaggcgtg
      961 ggccgggctt attgcgagga aacccacggc ggcaatgctg ctgcatcacc tcgtggcgca
     1021 gatgggccac cagaacgccg tggtggtcag ccagaagaca ctttccaagc tcatcggacg
     1081 ttctttgcgg acggtccaat acgcagtcaa ggacttggtg gccgagcgct ggatctccgt
     1141 cgtgaagctc aacggccccg gcaccgtgtc ggcctacgtg gtcaatgacc gcgtggcgtg
     1201 gggccagccc cgcgaccagt tgcgcctgtc ggtgttcagt gccgccgtgg tggttgatca
     1261 cgacgaccag gacgaatcgc tgttggggca tggcgacctg cgccgcatcc cgaccctgta
     1321 tccgggcgag cagcaactac cgaccggccc cggcgaggag ccgcccagcc agcccggcat
     1381 tccgggcatg gaaccagacc tgccagcctt gaccgaaacg gaggaatggg aacggcgcgg
     1441 gcagcagcgc ctgccgatgc ccgatgagcc gtgttttctg gacgatggcg agccgttgga
     1501 gccgccgaca cgggtcacgc tgccgcgccg gtagtacgta agaggttcca actttcacca
     1561 taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa
     1621 ggaagctaaa atgaaatcta acaatgcgct catcgtcatc ctcggcaccg tcaccctgga
     1681 tgctgtaggc ataggcttgg ttatgccggt actgccgggc ctcttgcggg atatcgtcca
     1741 ttccgacagc atcgccagtc actatggcgt gctgctagcg ctatatgcgt tgatgcaatt
     1801 tctatgcgca cccgttctcg gagcactgtc cgaccgcttt ggccgccgcc cagtcctgct
     1861 cgcttcgcta cttggagcca ctatcgacta cgcgatcatg gcgaccacac ccgtcctgtg
     1921 gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg
     1981 cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag
     2041 cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat
     2101 ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg
     2161 ctgcttccta atgcaggagt cgcataaggg agagcgtcga ccgatgccct tgagagcctt
     2221 caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac
     2281 tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctctggg tcattttcgg
     2341 cgaggaccgc tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat
     2401 cttgcacgcc ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa
     2461 gcaggccatt atcgccggca tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc
     2521 gacgcgaggc tggatggcct tccccattat gattcttctc gcttccggcg gcatcgggat
     2581 gcccgcgttg caggccatgc tgtccaggca ggtagatgac gaccatcagg gacagcttca
     2641 aggatcgctc gcggctctta ccagcctaac ttcgatcact ggaccgctga tcgtcacggc
     2701 gatttatgcc gcctcggcga gcacatggaa cgggttggca tggattgtag gcgccgccct
     2761 ataccttgtc tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg
     2821 aatggaaggg atccccctca agtcaaaagc ctccggtcgg aggcttttga ctttctgcta
     2881 tggaggtcag gtatgattta aatggtcagt attgagcgat atctagagaa ttcgtc
//

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