LOCUS pNeo 5508 bp DNA circular SYN 30-APR-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5508)
AUTHORS Ming
TITLE Direct Submission
JOURNAL Exported Apr 30, 2025 from SnapGene 6.0.2
https://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5508
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS 385..1179
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
CDS 3062..3253
/codon_start=1
/gene="rop"
/product="Rop protein, which maintains plasmids at low copy
number"
/label=rop
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
misc_feature 3355..3495
/label=bom
/note="basis of mobility region from pBR322"
rep_origin complement(3681..4269)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4440..5300)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5301..5405)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 ttctcatgtt tgacagctta tcatcgataa gcttcacgct gccgcaagca ctcagggcgc
61 aagggctgct aaaggaagcg gaacacgtag aaagccagtc cgcagaaacg gtgctgaccc
121 cggatgaatg tcagctactg ggctatctgg acaagggaaa acgcaagcgc aaagagaaag
181 caggtagctt gcagtgggct tacatggcga tagctagact gggcggtttt atggacagca
241 agcgaaccgg aattgccagc tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta
301 aactggatgg ctttcttgcc gccaaggatc tgatggcgca ggggatcaag atctgatcaa
361 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
421 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
481 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
541 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
601 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
661 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
721 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
781 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
841 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
901 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
961 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
1021 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
1081 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
1141 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
1201 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
1261 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
1321 gggatctcat gctggagttc ttcgcccacc ccgggctcga tcccctcgcg agttggttca
1381 gctgctgcct gaggctggac gacctcgcgg agttctaccg gcagtgcaaa tccgtcggca
1441 tccaggaaac cagcagcggc tatccgcgca tccatgcccc cgaactgcag gagtggggag
1501 gcacgatggc cgctttggtc ccggatcctc tacgccggac gcatcgtggc cggcatcacc
1561 ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg
1621 gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggcccgtgg
1681 ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca
1741 acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc
1801 gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga
1861 ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg
1921 cagcgctctg ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc
1981 tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca agccttcgtc actggtcccg
2041 ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg catggcggcc gacgcgctgg
2101 gctacgtctt gctggcgttc gcgacgcgag gctggatggc cttccccatt atgattcttc
2161 tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat gctgtccagg caggtagatg
2221 acgaccatca gggacagctt caaggatcgc tcgcggctct taccagccta acttcgatca
2281 ctggaccgct gatcgtcacg gcgatttatg ccgcctcggc gagcacatgg aacgggttgg
2341 catggattgt aggcgccgcc ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat
2401 ggagccgggc cacctcgacc tgaatggaag ccggcggcac ctcgctaacg gattcaccac
2461 tccaagaatt ggagccaatc aattcttgcg gagaactgtg aatgcgcaaa ccaacccttg
2521 gcagaacata tccatcgcgt ccgccatctc cagcagccgc acgcggcgca tctcgggcag
2581 cgttgggtcc tggccacggg tgcgcatgat cgtgctcctg tcgttgagga cccggctagg
2641 ctggcggggt tgccttactg gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag
2701 cgactgctgc tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct tcggtttccg
2761 tgtttcgtaa agtctggaaa cgcggaagtc agcgccctgc accattatgt tccggatctg
2821 catcgcagga tgctgctggc taccctgtgg aacacctaca tctgtattaa cgaagcgctg
2881 gcattgaccc tgagtgattt ttctctggtc ccgccgcatc cataccgcca gttgtttacc
2941 ctcacaacgt tccagtaacc gggcatgttc atcatcagta acccgtatcg tgagcatcct
3001 ctctcgtttc atcggtatca ttacccccat gaacagaaat cccccttaca cggaggcatc
3061 agtgaccaaa caggaaaaaa ccgcccttaa catggcccgc tttatcagaa gccagacatt
3121 aacgcttctg gagaaactca acgagctgga cgcggatgaa caggcagaca tctgtgaatc
3181 gcttcacgac cacgctgatg agctttaccg cagctgcctc gcgcgtttcg gtgatgacgg
3241 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
3301 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc
3361 catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag
3421 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
3481 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
3541 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
3601 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
3661 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
3721 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
3781 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
3841 gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt
3901 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
3961 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
4021 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
4081 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
4141 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
4201 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
4261 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
4321 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
4381 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
4441 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
4501 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
4561 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
4621 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
4681 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
4741 gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
4801 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
4861 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
4921 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
4981 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
5041 tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
5101 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
5161 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
5221 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
5281 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
5341 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
5401 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
5461 ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaagaa
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