pUCC001

LOCUS       pUCC001               10233 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Multi-species CRISPR/Cas9 coexpression system, optimized for Golden 
            Gate cloning of gRNA targets.
ACCESSION   .
VERSION     .
KEYWORDS    pUCC001
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10233)
  AUTHORS   Arun S. Rajkumar, Javier A. Varela, Hannes Juergens, Jean-Marc G. 
            Dara2, John P. Morrissey
  TITLE     Biological Parts for Kluyveromyces marxianus Synthetic Biology
  JOURNAL   Front. Bioeng. Biotechnol., 07 May 2019
REFERENCE   2  (bases 1 to 10233)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10233
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             696..719
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             723..743
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             744..4844
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTYKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSITHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4848..4868
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        5787..6453
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase"
     primer_bind     6323..6346
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     misc_RNA        6512..6587
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(6702..6720)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             complement(7154..8014)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7777..7796
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8015..8119)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8222..8810
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8711..8730
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             9322..117
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
ORIGIN
        1 gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa
       61 gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa ggaataactc
      121 gaggacaata aaaagattct tgttttcaag aacttgtcat ttgtatagtt tttttatatt
      181 gtagttgttc tattttaatc aaatgttagc gtgatttata ttttttttcg cctcgacatc
      241 atctgcccag atgcgaagtt aagtgcgcag aaagtaatat catgcgtcaa tcgtatgtga
      301 atgctggtcg ctatactgga gctctcgaga accctcacct ttcgagagga cgatgcccgt
      361 gtctaaatga ttcgaccagc ctaagaatgt tcaacaatct ataatcagtc catagtcaac
      421 aagagccctc aaagttgata tcgtttagtg atctccctcg agatgagcgg aggatggttg
      481 ctttttgggg agccctaagt ggtacgtgcg gcgagcggga tgtttcccca ttaggcaaag
      541 gacgaccggg taacccctcg agaaaaaaaa tttttcgccg ctaatctggt gttatataaa
      601 gctccccctg ctcctggaat ttttttcctt gtcaactcac accggaaatc aaggcatttc
      661 attctgagta gttcttaaaa acataatcaa caatggatta taaagatgac gatgacaaac
      721 ctccaaaaaa gaagagaaag gtcgataaga aatactcaat aggcttagat atcggcacaa
      781 atagcgtcgg atgggcggtg atcactgatg aatataaggt tccgtctaaa aagttcaagg
      841 ttctgggaaa tacagaccgc cacagtatca aaaaaaatct tataggggct cttttatttg
      901 acagtggaga gacagcggaa gcgactcgtc tcaaacggac agctcgtaga aggtatacac
      961 gtcggaagaa tcgtatttgt tatctacagg agattttttc aaatgagatg gcgaaagtag
     1021 atgatagttt ctttcatcga cttgaagagt cttttttggt ggaagaagac aagaagcatg
     1081 aacgtcatcc tatttttgga aatatagtag atgaagttgc ttatcatgag aaatatccaa
     1141 ctatctatca tctgcgaaaa aaattggtag attctactta taaagcggat ttgcgcttaa
     1201 tctatttggc cttagcgcat atgattaagt ttcgtggtca ttttttgatt gagggagatt
     1261 taaatcctga taatagtgat gtggacaaac tatttatcca gttggtacaa acctacaatc
     1321 aattatttga agaaaaccct attaacgcaa gtggagtaga tgctaaagcg attctttctg
     1381 cacgattgag taaatcaaga cgattagaaa atctcattgc tcagctcccc ggtgagaaga
     1441 aaaatggctt atttgggaat ctcattgctt tgtcattggg tttgacccct aattttaaat
     1501 caaattttga tttggcagaa gatgctaaat tacagctttc aaaagatact tacgatgatg
     1561 atttagataa tttattggcg caaattggag atcaatatgc tgatttgttt ttggcagcta
     1621 agaatttatc agatgctatt ttactttcag atatcctaag agtaaatact gaaataacta
     1681 aggctcccct atcagcttca atgattaaac gctacgatga acatcatcaa gacttgactc
     1741 ttttaaaagc tttagttcga caacaacttc cagaaaagta taaagaaatc ttttttgatc
     1801 aatcaaaaaa cggatatgca ggttatattg atgggggagc tagccaagaa gaattttata
     1861 aatttatcaa accaatttta gaaaaaatgg atggtactga ggaattattg gtgaaactaa
     1921 atcgtgaaga tttgctgcgc aagcaacgga cctttgacaa cggctctatt acccatcaaa
     1981 ttcacttggg tgagctgcat gctattttga gaagacaaga agacttttat ccatttttaa
     2041 aagacaatcg tgagaagatt gaaaaaatct tgacttttcg aattccttat tatgttggtc
     2101 cattggcgcg tggcaatagt cgttttgcat ggatgactcg gaagtctgaa gaaacaatta
     2161 ccccatggaa ttttgaagaa gttgtcgata aaggtgcttc agctcaatca tttattgaac
     2221 gcatgacaaa ctttgataaa aatcttccaa atgaaaaagt actaccaaaa catagtttgc
     2281 tttatgagta ttttacggtt tataacgaat tgacaaaggt caaatatgtt actgaaggaa
     2341 tgcgaaaacc agcatttctt tcaggtgaac agaagaaagc cattgttgat ttactcttca
     2401 aaacaaatcg aaaagtaacc gttaagcaat taaaagaaga ttatttcaaa aaaatagaat
     2461 gttttgatag tgttgaaatt tcaggagttg aagatagatt taatgcttca ttaggtacct
     2521 accatgattt gctaaaaatt attaaagata aagatttttt ggataatgaa gaaaatgaag
     2581 atatcttaga ggatattgtt ttaacattga ccttatttga agatagggag atgattgagg
     2641 aaagacttaa aacatatgct cacctctttg atgataaggt gatgaaacag cttaaacgtc
     2701 gccgttatac tggttgggga cgtttgtctc gaaaattgat taatggtatt agggataagc
     2761 aatctggcaa aacaatatta gattttttga aatcagatgg ttttgccaat cgcaatttta
     2821 tgcagctgat ccatgatgat agtttgacat ttaaagaaga cattcaaaaa gcacaagtgt
     2881 ctggacaagg cgatagttta catgaacata ttgcaaattt agctggtagc cctgctatta
     2941 aaaaaggtat tttacagact gtaaaagttg ttgatgaatt ggtcaaagta atggggcggc
     3001 ataagccaga aaatatcgtt attgaaatgg cacgtgaaaa tcagacaact caaaagggcc
     3061 agaaaaattc gcgagagcgt atgaaacgaa tcgaagaagg tatcaaagaa ttaggaagtc
     3121 agattcttaa agagcatcct gttgaaaata ctcaattgca aaatgaaaag ctctatctct
     3181 attatctcca aaatggaaga gacatgtatg tggaccaaga attagatatt aatcgtttaa
     3241 gtgattatga tgtcgatcac attgttccac aaagtttcct taaagacgat tcaatagaca
     3301 ataaggtctt aacgcgttct gataaaaatc gtggtaaatc ggataacgtt ccaagtgaag
     3361 aagtagtcaa aaagatgaaa aactattgga gacaacttct aaacgccaag ttaatcactc
     3421 aacgtaagtt tgataattta acgaaagctg aacgtggagg tttgagtgaa cttgataaag
     3481 ctggttttat caaacgccaa ttggttgaaa ctcgccaaat cactaagcat gtggcacaaa
     3541 ttttggatag tcgcatgaat actaaatacg atgaaaatga taaacttatt cgagaggtta
     3601 aagtgattac cttaaaatct aaattagttt ctgacttccg aaaagatttc caattctata
     3661 aagtacgtga gattaacaat taccatcatg cccatgatgc gtatctaaat gccgtcgttg
     3721 gaactgcttt gattaagaaa tatccaaaac ttgaatcgga gtttgtctat ggtgattata
     3781 aagtttatga tgttcgtaaa atgattgcta agtctgagca agaaataggc aaagcaaccg
     3841 caaaatattt cttttactct aatatcatga acttcttcaa aacagaaatt acacttgcaa
     3901 atggagagat tcgcaaacgc cctctaatcg aaactaatgg ggaaactgga gaaattgtct
     3961 gggataaagg gcgagatttt gccacagtgc gcaaagtatt gtccatgccc caagtcaata
     4021 ttgtcaagaa aacagaagta cagacaggcg gattctccaa ggagtcaatt ttaccaaaaa
     4081 gaaattcgga caagcttatt gctcgtaaaa aagactggga tccaaaaaaa tatggtggtt
     4141 ttgatagtcc aacggtagct tattcagtcc tagtggttgc taaggtggaa aaagggaaat
     4201 cgaagaagtt aaaatccgtt aaagagttac tagggatcac aattatggaa agaagttcct
     4261 ttgaaaaaaa tccgattgac tttttagaag ctaaaggata taaggaagtt aaaaaagact
     4321 taatcattaa actacctaaa tatagtcttt ttgagttaga aaacggtcgt aaacggatgc
     4381 tggctagtgc cggagaatta caaaaaggaa atgagctggc tctgccaagc aaatatgtga
     4441 attttttata tttagctagt cattatgaaa agttgaaggg tagtccagaa gataacgaac
     4501 aaaaacaatt gtttgtggag cagcataagc attatttaga tgagattatt gagcaaatca
     4561 gtgaattttc taagcgtgtt attttagcag atgccaattt agataaagtt cttagtgcat
     4621 ataacaaaca tagagacaaa ccaatacgtg aacaagcaga aaatattatt catttattta
     4681 cgttgacgaa tcttggagct cccgctgctt ttaaatattt tgatacaaca attgatcgta
     4741 aacgatatac gtctacaaaa gaagttttag atgccactct tatccatcaa tccatcactg
     4801 gtctttatga aacacgcatt gatttgagtc agctaggagg tgaccctcca aaaaagaaga
     4861 gaaaggtctg agcggttttg tataactaaa taatattgga aactaaatac gaatacccaa
     4921 attttttatc taaattttgc cgaaagatta aaatctgcag agatatccga aacaggtaaa
     4981 tggatgtttc aatccctgta gtcagtcagg aacccatatt atattacagt attagtcgcc
     5041 gcttaggcac gcctttaatt agcaaaatca aaccttaagt gcatatgccg tataagggaa
     5101 actcaaagaa ctggcatcgc aaaaatgaaa acatacgttg aaactacggc aaaggattgg
     5161 tcagatcgct tcatacaggg aaagttcggc atcaacatct ttggataata tcagaatgag
     5221 aaagaacaga tacgcagtac gttttttggt gagctctttg cacttcttta gttctttcca
     5281 tcaatatcag ttttttaaac ttttaggact aaaagtgatg tttaacttca aaatgtttaa
     5341 aattttgttc ttcccgacgt tcattaagaa tactaataca ctttaataat tattttaata
     5401 ttttgttcta aataatgact tttaattaaa aaagataaaa tataaaaaca tcataataac
     5461 tcaccagagg ttaagaacaa aaaaacaaat tagatatctg ctaatccaat atagttaaat
     5521 caatctttcc ttggtataat gggtatatta catatatttc aaggaccgac actcctacca
     5581 aatatctaaa atttaccata ttaacataac atgtatataa acgtcaaatc ataatcagca
     5641 ctaacgtctc acggatcgta tatgccgtag cgacaatcta agaactatgc gaggacacgc
     5701 tagggccaag gcaaaaagat tccttgatta cgtaagggag ttagaatcat tttgaataaa
     5761 aaacacgctt tttcagttcg agtttatcat tatcaatact gccatttcaa agaatacgta
     5821 aataattaat agtagtgatt ttcctaactt tatttagtca aaaaattagc cttttaattc
     5881 tgctgtaacc cgtacatgcc caaaataggg ggcgggttac acagaatata taacatcgta
     5941 ggtgtctggg tgaacagttt attcctggca tccactaaat ataatggagc ccgcttttta
     6001 agctggcatc cagaaaaaaa aagaatccca gcaccaaaat attgttttct tcaccaacca
     6061 tcagttcata ggtccattct cttagcgcaa ctacagagaa caggggcaca aacaggcaaa
     6121 aaacgggcac aacctcaatg gagtgatgca acctgcctgg agtaaatgat gacacaaggc
     6181 aattgaccca cgcatgtatc tatctcattt tcttacacct tctattacct tctgctctct
     6241 ctgatttgga aaaagctgaa aaaaaaggtt gaaaccagtt ccctgaaatt attcccctac
     6301 ttgactaata agtatataaa gacggtaggt attgattgta attctgtaaa tctatttctt
     6361 aaacttctta aattctactt ttatagttag tctttttttt agttttaaaa caccaagaac
     6421 ttagtttcga ataaacacac ataaacaaac aaacctgatg agtccgtgag gacgaaacga
     6481 gtaagctcgt ccgagacctg cggaggtctc cgttttagag ctagaaatag caagttaaaa
     6541 taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt tggccggcat
     6601 ggtcccagcc tcctcgctgg cgccggctgg gcaacatgct tcggcatggc gaatgggacc
     6661 acaggcccct tttcctttgt cgatatcatg taattagtta tgtcacgctt acattcacgc
     6721 cctcccccca catccgctct aaccgaaaag gaaggagtta gacaacctga agtctaggtc
     6781 cctatttatt tttttatagt tatgttagta ttaagaacgt tatttatatt tcaaattttt
     6841 cttttttttc tgtacaaacg cgtgtacgca tgtaacatta tactgaaaac cttgcttgag
     6901 aaggttttgg gacaagctta cgtctcacgg atcgtatatg ccgtagcgac aatctaagaa
     6961 ctatgcgagg acacgctagc agctgtattc acgtagacgg ataggtatag ccagacatca
     7021 gcagcatact tcgggaaccg taggccaccg agcggccgcg attatcaaaa aggatcttca
     7081 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     7141 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     7201 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     7261 taccatctgg ccccagtgct gcaatgatac cgcgggaccc acgctcaccg gctccagatt
     7321 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     7381 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     7441 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     7501 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     7561 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     7621 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     7681 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     7741 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     7801 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     7861 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     7921 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     7981 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     8041 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     8101 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgtcatg accaaaatcc
     8161 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     8221 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     8281 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     8341 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     8401 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     8461 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     8521 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     8581 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     8641 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     8701 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     8761 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     8821 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     8881 cgttatcccc tgattctgtg gataaccgtg cggactatat gtgaaggcat ggctatggca
     8941 cggcagacat tccgccagat catcaatagg cacgacatgg aggcccagaa taccctcctt
     9001 gacagtcttg acgtgcgcag ctcaggggca tgatgtgact gtcgcccgta catttagccc
     9061 atacatcccc atgtataatc atttgcatcc atacattttg atggccgcac ggcgcgaagc
     9121 aaaaattacg gctcctcgct gcagacctgc gagcagggaa acgctcccct cacagacgcg
     9181 ttgaattgtc cccacgccgc gcccctgtag agaaatataa aaggttagga tttgccactg
     9241 aggttcttct ttcatatact tccttttaaa atcttgctag gatacagttc tcacatcaca
     9301 tccgaacata aacaaggatc catgggtaaa aagcctgaac tcaccgcgac gtctgtcgag
     9361 aagtttctga tcgaaaagtt cgacagcgtg tccgacctga tgcagctctc ggagggcgaa
     9421 gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc
     9481 tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc
     9541 ccgattccgg aagtgcttga cattggggaa tttagcgaga gcctgaccta ttgcatctcc
     9601 cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg
     9661 caaccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca gacgagcggg
     9721 ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc
     9781 gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg
     9841 tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg
     9901 cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca
     9961 gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc
    10021 ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg
    10081 catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat tggtcttgac
    10141 caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga
    10201 tgcgacgcaa tcgtccgatc cggagccggg act
//