| 质粒名称: | pCMV-MMLVgag-3xNES-ABE8e |
|---|---|
| 目录编号: | 181751 |
| 存储实验室: | David Liu |
|
载体骨架基本信息 |
|
| 载体名称: | pCDNA3-like |
| 空载体大小: | 4757 bp |
| 完整质粒大小: | 11365bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | Ampicillin, 100 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | MMLVgag-3xNES-ABE8e |
| 物种来源: | H. sapiens (human), M. musculus (mouse) |
| 插入大小: | 6609 bp |
| 启动子: | CMV |
| 标签/融合蛋白: | Flag (N terminal on insert) |
| 克隆方法: | Ligation Independent Cloning |
| 5’测序引物 | ACCCTGGTCATCATCCTG |
| 3’测序引物 | CAGGAAACAGCTATGAC; CCTTCCGAGTGAGAGAC |
|
文献引用方法 |
|
| 材料和方法部分: | pCMV-MMLVgag-3xNES-ABE8e was a gift from David Liu (Addgene plasmid # 181751 ; http://n2t.net/addgene:181751 ; RRID:Addgene_181751) |
| 参考文献部分: | Engineered virus-like particles for efficient in vivo delivery of therapeutic proteins. Banskota S, Raguram A, Suh S, Du SW, Davis JR, Choi EH, Wang X, Nielsen SC, Newby GA, Randolph PB, Osborn MJ, Musunuru K, Palczewski K, Liu DR. Cell. 2022 Jan 7. pii: S0092-8674(21)01484-7. doi: 10.1016/j.cell.2021.12.021. 10.1016/j.cell.2021.12.021 PubMed 35 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCMV-MMLVgag-3xNES-ABE8e 11365 bp ds-DNA circular SYN 08-FEB-2022
DEFINITION MMLV-Gag fused to 3xNES-ABE8e with TSTLLMENSS cleavage site between
Gag-NES and ABE. For production of virus like particles with
base-editor RNP cargo..
ACCESSION .
VERSION .
KEYWORDS pCMV-MMLVgag-3xNES-ABE8e
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11365)
AUTHORS Banskota S, Raguram A, Suh S, Du SW, Davis JR, Choi EH, Wang X,
Nielsen SC, Newby GA, Randolph PB, Osborn MJ, Musunuru K, Palczewski
K, Liu DR
TITLE Engineered virus-like particles for efficient in vivo delivery of
therapeutic proteins.
JOURNAL Cell. 2022 Jan 7. pii: S0092-8674(21)01484-7. doi:
10.1016/j.cell.2021.12.021.
PUBMED 35021064
REFERENCE 2 (bases 1 to 11365)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Feb 8, 2022 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..11365
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 4..420
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 350..372
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 388..404
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
CDS 1633..1659
/codon_start=1
/product="nuclear export signal from the HIV Rev protein
(Fischer et al., 1995)"
/label=NES
/translation="LPPLERLTL"
CDS 1672..1698
/codon_start=1
/product="nuclear export signal from the HIV Rev protein
(Fischer et al., 1995)"
/label=NES
/translation="LPPLERLTL"
CDS 1711..1737
/codon_start=1
/product="nuclear export signal from the HIV Rev protein
(Fischer et al., 1995)"
/label=NES
/translation="LPPLERLTL"
CDS 1771..1794
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
CDS 1828..1848
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 2443..6543
/codon_start=1
/product="nickase mutant of the Cas9 endonuclease from the
Streptococcus pyogenes Type II CRISPR/Cas system"
/label=Cas9(D10A)
/note="generates RNA-guided single strand nicks in DNA due
to the D10A mutation in the RuvC catalytic domain"
/translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS 6586..6606
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
primer_bind complement(6612..6631)
/label=Bglob-pA-R
/note="Rabbit beta-globin polyA region, reverse primer"
polyA_signal 6677..6732
/label=-beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
primer_bind complement(6731..6750)
/label=rbglobpA-R
/note="Rabbit beta-globin polyA, reverse primer. Also
called rb-glob-pA-term-R"
primer_bind complement(7090..7106)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7090..7106)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(7103..7125)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 7114..7130
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7138..7168)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7183..7204
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(7321..7338)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(7492..8080)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(7572..7591)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(8251..9111)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8874..8893
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9112..9216)
/gene="bla"
/label=AmpR promoter
rep_origin complement(9242..9697)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(9379..9400)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 9591..9610
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 9824..9846
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 9838..9855
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 9839..9855
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 9865..9884
/label=T7
/note="T7 promoter, forward primer"
promoter 9865..9883
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
enhancer 10004..10383
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 10384..10587
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 10538..10558
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 10584..10608
/label=LNCX
/note="Human CMV promoter, forward primer"
intron 10699..11271
/label=-beta-globin intron
/note="intron from rabbit beta-globin gene"
ORIGIN
1 atgggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa agatgtcgag
61 cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac cttctgctct
121 gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa ccgagacctc
181 atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc agaccaggtc
241 ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt caagcccttt
301 gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc ccttgaacct
361 cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc tctaggcgcc
421 aaacctaaac ctcaagttct ttctgacagt ggggggccgc tcatcgacct acttacagaa
481 gaccccccgc cttataggga cccaagacca cccccttccg acagggacgg aaatggtgga
541 gaagcgaccc ctgcgggaga ggcaccggac ccctccccaa tggcatctcg cctacgtggg
601 agacgggagc cccctgtggc cgactccact acctcgcagg cattccccct ccgcgcagga
661 ggaaacggac agcttcaata ctggccgttc tcctcttctg acctttacaa ctggaaaaat
721 aataaccctt ctttttctga agatccaggt aaactgacag ctctgatcga gtctgtcctc
781 atcacccatc agcccacctg ggacgactgt cagcagctgt tggggactct gctgaccgga
841 gaagaaaaac aacgggtgct cttagaggct agaaaggcgg tgcggggcga tgatgggcgc
901 cccactcaac tgcccaatga agtcgatgcc gcttttcccc tcgagcgccc agactgggat
961 tacaccaccc aggcaggtag gaaccaccta gtccactatc gccagttgct cctagcgggt
1021 ctccaaaacg cgggcagaag ccccaccaat ttggccaagg taaaaggaat aacacaaggg
1081 cccaatgagt ctccctcggc cttcctagag agacttaagg aagcctatcg caggtacact
1141 ccttatgacc ctgaggaccc agggcaagaa actaatgtgt ctatgtcttt catttggcag
1201 tctgccccag acattgggag aaagttagag aggttagaag atttaaaaaa caagacgctt
1261 ggagatttgg ttagagaggc agaaaagatc tttaataaac gagaaacccc ggaagaaaga
1321 gaggaacgta tcaggagaga aacagaggaa aaagaagaac gccgtaggac agaggatgag
1381 cagaaagaga aagaaagaga tcgtaggaga catagagaga tgagcaagct attggccact
1441 gtcgttagtg gacagaaaca ggatagacag ggaggagaac gaaggaggtc ccaactcgat
1501 cgcgaccagt gtgcctactg caaagaaaag gggcactggg ctaaagattg tcccaagaaa
1561 ccacgaggac ctcggggacc aagaccccag acctccctcc tgaccctaga tgactctggc
1621 ggctcacttc aactgcctcc acttgaaaga ctgacactgg gatcattaca attacctcct
1681 ttagaacgat taacactcgg ttcactacag cttccgcctc ttgagagatt gacattaacg
1741 tccacgctgc taatggagaa ctcgtctgga gattacaaag acgatgacga taagaaacgg
1801 acagccgacg gaagcgagtt cgagtcacca aagaagaagc ggaaagtctc tgaggtggag
1861 ttttcccacg agtactggat gagacatgcc ctgaccctgg ccaagagggc acgggatgag
1921 agggaggtgc ctgtgggagc cgtgctggtg ctgaacaata gagtgatcgg cgagggctgg
1981 aacagagcca tcggcctgca cgacccaaca gcccatgccg aaattatggc cctgagacag
2041 ggcggcctgg tcatgcagaa ctacagactg attgacgcca ccctgtacgt gacattcgag
2101 ccttgcgtga tgtgcgccgg cgccatgatc cactctagga tcggccgcgt ggtgtttggc
2161 gtgaggaact caaaaagagg cgccgcaggc tccctgatga acgtgctgaa ctaccccggc
2221 atgaatcacc gcgtcgaaat taccgaggga atcctggcag atgaatgtgc cgccctgctg
2281 tgcgatttct atcggatgcc tagacaggtg ttcaatgctc agaagaaggc ccagagctcc
2341 atcaactccg gaggatctag cggaggctcc tctggctctg agacacctgg cacaagcgag
2401 agcgcaacac ctgaaagcag cgggggcagc agcggggggt cagacaagaa gtacagcatc
2461 ggcctggcca tcggcaccaa ctctgtgggc tgggccgtga tcaccgacga gtacaaggtg
2521 cccagcaaga aattcaaggt gctgggcaac accgaccggc acagcatcaa gaagaacctg
2581 atcggagccc tgctgttcga cagcggcgaa acagccgagg ccacccggct gaagagaacc
2641 gccagaagaa gatacaccag acggaagaac cggatctgct atctgcaaga gatcttcagc
2701 aacgagatgg ccaaggtgga cgacagcttc ttccacagac tggaagagtc cttcctggtg
2761 gaagaggata agaagcacga gcggcacccc atcttcggca acatcgtgga cgaggtggcc
2821 taccacgaga agtaccccac catctaccac ctgagaaaga aactggtgga cagcaccgac
2881 aaggccgacc tgcggctgat ctatctggcc ctggcccaca tgatcaagtt ccggggccac
2941 ttcctgatcg agggcgacct gaaccccgac aacagcgacg tggacaagct gttcatccag
3001 ctggtgcaga cctacaacca gctgttcgag gaaaacccca tcaacgccag cggcgtggac
3061 gccaaggcca tcctgtctgc cagactgagc aagagcagac ggctggaaaa tctgatcgcc
3121 cagctgcccg gcgagaagaa gaatggcctg ttcggaaacc tgattgccct gagcctgggc
3181 ctgaccccca acttcaagag caacttcgac ctggccgagg atgccaaact gcagctgagc
3241 aaggacacct acgacgacga cctggacaac ctgctggccc agatcggcga ccagtacgcc
3301 gacctgtttc tggccgccaa gaacctgtcc gacgccatcc tgctgagcga catcctgaga
3361 gtgaacaccg agatcaccaa ggcccccctg agcgcctcta tgatcaagag atacgacgag
3421 caccaccagg acctgaccct gctgaaagct ctcgtgcggc agcagctgcc tgagaagtac
3481 aaagagattt tcttcgacca gagcaagaac ggctacgccg gctacattga cggcggagcc
3541 agccaggaag agttctacaa gttcatcaag cccatcctgg aaaagatgga cggcaccgag
3601 gaactgctcg tgaagctgaa cagagaggac ctgctgcgga agcagcggac cttcgacaac
3661 ggcagcatcc cccaccagat ccacctggga gagctgcacg ccattctgcg gcggcaggaa
3721 gatttttacc cattcctgaa ggacaaccgg gaaaagatcg agaagatcct gaccttccgc
3781 atcccctact acgtgggccc tctggccagg ggaaacagca gattcgcctg gatgaccaga
3841 aagagcgagg aaaccatcac cccctggaac ttcgaggaag tggtggacaa gggcgcttcc
3901 gcccagagct tcatcgagcg gatgaccaac ttcgataaga acctgcccaa cgagaaggtg
3961 ctgcccaagc acagcctgct gtacgagtac ttcaccgtgt ataacgagct gaccaaagtg
4021 aaatacgtga ccgagggaat gagaaagccc gccttcctga gcggcgagca gaaaaaggcc
4081 atcgtggacc tgctgttcaa gaccaaccgg aaagtgaccg tgaagcagct gaaagaggac
4141 tacttcaaga aaatcgagtg cttcgactcc gtggaaatct ccggcgtgga agatcggttc
4201 aacgcctccc tgggcacata ccacgatctg ctgaaaatta tcaaggacaa ggacttcctg
4261 gacaatgagg aaaacgagga cattctggaa gatatcgtgc tgaccctgac actgtttgag
4321 gacagagaga tgatcgagga acggctgaaa acctatgccc acctgttcga cgacaaagtg
4381 atgaagcagc tgaagcggcg gagatacacc ggctggggca ggctgagccg gaagctgatc
4441 aacggcatcc gggacaagca gtccggcaag acaatcctgg atttcctgaa gtccgacggc
4501 ttcgccaaca gaaacttcat gcagctgatc cacgacgaca gcctgacctt taaagaggac
4561 atccagaaag cccaggtgtc cggccagggc gatagcctgc acgagcacat tgccaatctg
4621 gccggcagcc ccgccattaa gaagggcatc ctgcagacag tgaaggtggt ggacgagctc
4681 gtgaaagtga tgggccggca caagcccgag aacatcgtga tcgaaatggc cagagagaac
4741 cagaccaccc agaagggaca gaagaacagc cgcgagagaa tgaagcggat cgaagagggc
4801 atcaaagagc tgggcagcca gatcctgaaa gaacaccccg tggaaaacac ccagctgcag
4861 aacgagaagc tgtacctgta ctacctgcag aatgggcggg atatgtacgt ggaccaggaa
4921 ctggacatca accggctgtc cgactacgat gtggaccata tcgtgcctca gagctttctg
4981 aaggacgact ccatcgacaa caaggtgctg accagaagcg acaagaaccg gggcaagagc
5041 gacaacgtgc cctccgaaga ggtcgtgaag aagatgaaga actactggcg gcagctgctg
5101 aacgccaagc tgattaccca gagaaagttc gacaatctga ccaaggccga gagaggcggc
5161 ctgagcgaac tggataaggc cggcttcatc aagagacagc tggtggaaac ccggcagatc
5221 acaaagcacg tggcacagat cctggactcc cggatgaaca ctaagtacga cgagaatgac
5281 aagctgatcc gggaagtgaa agtgatcacc ctgaagtcca agctggtgtc cgatttccgg
5341 aaggatttcc agttttacaa agtgcgcgag atcaacaact accaccacgc ccacgacgcc
5401 tacctgaacg ccgtcgtggg aaccgccctg atcaaaaagt accctaagct ggaaagcgag
5461 tttgtgtacg gcgactacaa ggtgtacgac gtgcggaaga tgatcgccaa gagcgagcag
5521 gaaatcggca aggctaccgc caagtacttc ttctacagca acatcatgaa ctttttcaag
5581 accgagatta ccctggccaa cggcgagatc cggaagcggc ctctgatcga gacaaacggc
5641 gaaaccgggg agatcgtgtg ggataagggc cgggattttg ccaccgtgcg gaaagtgctg
5701 agcatgcccc aagtgaatat cgtgaaaaag accgaggtgc agacaggcgg cttcagcaaa
5761 gagtctatcc tgcccaagag gaacagcgat aagctgatcg ccagaaagaa ggactgggac
5821 cctaagaagt acggcggctt cgacagcccc accgtggcct attctgtgct ggtggtggcc
5881 aaagtggaaa agggcaagtc caagaaactg aagagtgtga aagagctgct ggggatcacc
5941 atcatggaaa gaagcagctt cgagaagaat cccatcgact ttctggaagc caagggctac
6001 aaagaagtga aaaaggacct gatcatcaag ctgcctaagt actccctgtt cgagctggaa
6061 aacggccgga agagaatgct ggcctctgcc ggcgaactgc agaagggaaa cgaactggcc
6121 ctgccctcca aatatgtgaa cttcctgtac ctggccagcc actatgagaa gctgaagggc
6181 tcccccgagg ataatgagca gaaacagctg tttgtggaac agcacaagca ctacctggac
6241 gagatcatcg agcagatcag cgagttctcc aagagagtga tcctggccga cgctaatctg
6301 gacaaagtgc tgtccgccta caacaagcac cgggataagc ccatcagaga gcaggccgag
6361 aatatcatcc acctgtttac cctgaccaat ctgggagccc ctgccgcctt caagtacttt
6421 gacaccacca tcgaccggaa gaggtacacc agcaccaaag aggtgctgga cgccaccctg
6481 atccaccaga gcatcaccgg cctgtacgag acacggatcg acctgtctca gctgggaggt
6541 gactctggcg gctcaaaaag aaccgccgac ggcagcgaat tcgagcccaa gaagaagagg
6601 aaagtctgag atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag
6661 catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt
6721 ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca tcagaatgag
6781 tatttggttt agagtttggc aacatatgcc catatgctgg ctgccatgaa caaaggttgg
6841 ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt attccataga
6901 aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt tttttcttta
6961 acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct ctcctgacta
7021 ctcccagtca tagctgtccc tcttctctta tggagatccc tcgacggatc ggccgcaatt
7081 cgtaaccatg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
7141 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
7201 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
7261 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
7321 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
7381 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
7441 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
7501 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
7561 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
7621 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
7681 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
7741 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
7801 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
7861 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
7921 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
7981 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
8041 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
8101 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
8161 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
8221 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
8281 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
8341 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
8401 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
8461 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
8521 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
8581 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
8641 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
8701 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
8761 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
8821 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
8881 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
8941 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
9001 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
9061 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
9121 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
9181 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
9241 taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
9301 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
9361 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
9421 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
9481 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
9541 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
9601 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
9661 acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctgcgc
9721 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
9781 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
9841 aaaacgacgg ccagtgagcg cgcgtaatac gactcactat agggcgaatt ggagctccac
9901 cgcggtggcg gccgctctag agagcttggc ccattgcata cgttgtatcc atatcataat
9961 atgtacattt atattggctc atgtccaaca ttaccgccat gttgacattg attattgact
10021 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
10081 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
10141 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
10201 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
10261 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
10321 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
10381 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
10441 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
10501 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
10561 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc
10621 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ccggtcgacc
10681 gatcctgaga acttcagggt gagtttgggg acccttgatt gttctttctt tttcgctatt
10741 gtaaaattca tgttatatgg agggggcaaa gttttcaggg tgttgtttag aatgggaaga
10801 tgtcccttgt atcaccatgg accctcatga taattttgtt tctttcactt tctactctgt
10861 tgacaaccat tgtctcctct tattttcttt tcattttctg taactttttc gttaaacttt
10921 agcttgcatt tgtaacgaat ttttaaattc acttttgttt atttgtcaga ttgtaagtac
10981 tttctctaat cacttttttt tcaaggcaat cagggtatat tatattgtac ttcagcacag
11041 ttttagagaa caattgttat aattaaatga taaggtagaa tatttctgca tataaattct
11101 ggctggcgtg gaaatattct tattggtaga aacaactaca ccctggtcat catcctgcct
11161 ttctctttat ggttacaatg atatacactg tttgagatga ggataaaata ctctgagtcc
11221 aaaccgggcc cctctgctaa ccatgttcat gccttcttct ctttcctaca gctcctgggc
11281 aacgtgctgg ttgttgtgct gtctcatcat tttggcaaag aattcctcga gggccggatc
11341 cgaattcctg tatttgtctg aaaac
//
