pTet-Splice

价格:6500元

联系方式:I47-825O-882O

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pTetSplice载体基本信息

出品公司: Thermofisher
载体名称: pTet-Splice
质粒类型: 哺乳动物细胞载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: Tet
载体大小: 5.2 kb
5' 测序引物及序列: pBluescriptKS primer:TCGAGGTCGACGGTATC 
3' 测序引物及序列: pBluescriptSK Primer:TCTAGAACTAGTGGATC
载体抗性: 氨苄青霉素
克隆菌株: DH5α 等
宿主细胞(系): 常规细胞系(293、CV-1、CHO等)
备注: 这个质粒含Tet操纵子,这个操纵子控制质粒上面的CMV启动子,所以当这个质粒与激活质粒pTet-tTAK共表达的时候,目的蛋白可以在Doxycycline多西环素(一种四环素)的诱导下进行表达。所以这个质粒是哺乳细胞四环素诱导表达质粒。
产品目录号: #217568
稳定性: 稳表达
组成型/诱导型: 可诱导
病毒/非病毒: 非病毒

pTetSplice载体质粒图谱和多克隆位点信息

pTet-Splice载体图谱
pTet-Splice载体图谱

pTetSplice载体简介

pTetSplice载体序列

LOCUS       pTet-Splice               5178 bp ds-DNA     circular SYN 14-FEB-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    Untitled 2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5178)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, February 14, 2019 from SnapGene Viewer 3.2.1
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..5178
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    15..285
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tetracycline response element"
                     /note="contains seven copies of the tetracycline 
                      operator tetO"
     promoter        318..355
                     /note="minimal CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     484..500
                     /note="KS primer"
                     /note="common sequencing primer, one of multiple 
                      similar variants"
     primer_bind     complement(534..550)
                     /note="SK primer"
                     /note="common sequencing primer, one of multiple 
                      similar variants"
     intron          753..818
                     /note="small t intron"
                     /note="simian virus 40 (SV40) small t antigen intron"
     CDS             948..968
                     /codon_start=1
                     /product="nuclear localization signal of SV40 
                      large T antigen"
                     /note="SV40 NLS"
                     /translation="PKKKRKV"
     polyA_signal    1393..1527
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        complement(2319..2337)
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2347..2363)
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple 
                      similar variants"
     rep_origin      2505..2960
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; 
                     arrow indicates direction of (+) strand synthesis"
     promoter        2986..3090
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             3091..3951
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, 
                      and related antibiotics"                     
     rep_origin      4122..4710
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4998..5019
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the 
                      presence of cAMP."
     promoter        5034..5064
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    5072..5088
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator 
                      to inhibit transcription in E. coli. 
                       This inhibition can be relieved by adding 
                      lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5096..5112
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple 
                      similar variants"
     promoter        5133..5151
                     /note="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ctcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
       61 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
      121 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
      181 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
      241 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
      301 ctcggtaccc gggtcgagta ggcgtgtacg gtgggaggcc tatataagca gagctcgttt
      361 agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca
      421 ccgggaccga tccagcctcc gcggccccga attcgagctc ggtacccggg gatcctctag
      481 agtcgaggtc gacggtatcg ataagcttga tatcgaattc ctgcagcccg ggggatccac
      541 tagttctaga gcactgcgat gagtggcagg gcggggcgta atttttttaa ggcagttatt
      601 ggtgccctta aacgcctggt gctacgcctg aataagtgat aataagcgga tgaatggcag
      661 aaattcgccg gaggcggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg
      721 acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat
      781 gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg
      841 aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca gaagaaatgc
      901 catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca aaaaagaaga
      961 gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg agtcatgctg
     1021 tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa aaagctgcac
     1081 tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg cataacagtt
     1141 ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct gctattaata
     1201 actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt aataaggaat
     1261 atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt tgtagaggtt
     1321 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     1381 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     1441 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     1501 atcaatgtat cttatcatgt ctggatcccc aggaagctcc tctgtgtcct cataaaccct
     1561 aacctcctct acttgagagg acattccaat cataggctgc ccatccaccc tctgtgtcct
     1621 cctgttaatt aggtcactta acaaaaagga aattgggtag gggtttttca cagaccgctt
     1681 tctaagggta attttaaaat atctgggaag tcccttccac tgctgtgttc cagaagtgtt
     1741 ggtaaacagc ccacaaatgt caacagcaga aacatacaag ctgtcagctt tgcacaaggg
     1801 cccaacaccc tgctcatcaa gaagcactgt ggttgctgtg ttagtaatgt gcaaaacagg
     1861 aggcacattt tccccacctg tgtaggttcc aaaatatcta gtgttttcat ttttacttgg
     1921 atcaggaacc cagcactcca ctggataagc attatcctta tccaaaacag ccttgtggtc
     1981 agtgttcatc tgctgactgt caactgtagc attttttggg gttacagttt gagcaggata
     2041 tttggtcctg tagtttgcta acacaccctg cagctccaaa ggttccccac caacagcaaa
     2101 aaaatgaaaa tttgaccctt gaatgggttt tccagcacca ttttcatgag ttttttgtgt
     2161 ccctgaatgc aagtttaaca tagcagttac cccaataacc tcagttttaa cagtaacagc
     2221 ttcccacatc aaaatatttc cacaggttaa gtcctcattt aaattaggca aaggaattgc
     2281 tctagagcgg ccgccaccgc ggtggagctc caattcgccc tatagtgagt cgtattacgc
     2341 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     2401 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     2461 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg
     2521 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     2581 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     2641 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     2701 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     2761 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     2821 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     2881 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     2941 aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     3001 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     3061 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     3121 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     3181 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     3241 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     3301 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     3361 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     3421 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     3481 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     3541 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     3601 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     3661 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     3721 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     3781 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     3841 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     3901 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     3961 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     4021 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     4081 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     4141 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     4201 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     4261 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     4321 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     4381 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     4441 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     4501 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     4561 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     4621 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     4681 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     4741 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     4801 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     4861 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     4921 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     4981 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     5041 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     5101 aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag
     5161 ctgggtaccg ggcccccc
//

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pTetSplice载体应用举例

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