pBad43

价格:800元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Beckwith Lab
载体名称: pBad43, pBad 43
质粒类型: 大肠杆菌蛋白表达载体
表达水平:
启动子: araC
克隆方法: 多克隆位点,限制性内切酶
载体大小: 6179 bp
5' 测序引物及序列: pBAD-F: ATGCCATAGCATTTTTATCC
3' 测序引物及序列: pBAD-R: gatttaatctgtatcagg
载体标签: /
载体抗性: 壮观霉素
备注: Beckwith实验室构建,来源于pBad22。表达启动子是arabinose Bad启动子。
产品目录号: --
稳定性: 瞬时表达 Transient
组成型: 诱导表达
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pbad43载体图谱

载体简介

载体序列

LOCUS       pBAD43	6179 bp 	DNA     circular	SYN
DEFINITION  pBAD43
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     source          1..6179
                     /organism="pBAD43"
                     /mol_type="other DNA"
     misc_feature    complement(191..1069)
                     /label="AraC"
     CDS             complement(191..1120)
                     /label="ORF frame 2"
     misc_feature    1099..1114
                     /label="araO2"
     misc_feature    1256..1267
                     /label="araO1"
     misc_feature    1298..1311
                     /label="CAP_BS"
     misc_feature    1303..1322
                     /label="pBAD_fwd_primer"
     misc_feature    1308..1346
                     /label="AraI1I2"
     promoter        1343..1371
                     /label="ARA_promoter"
     misc_feature    complement(1494..1511)
                     /label="pTrcHis_rev_primer"
     misc_feature    complement(1494..1511)
                     /label="pBAD_rev_primer"
     terminator      1544..1701
                     /label="rrnB_terminator"
     terminator      1842..1869
                     /label="rrnB_T2_terminator"
     promoter        1910..1938
                     /label="AmpR_promoter"
     promoter        complement(1950..1966)
                     /label="M13_forward20_primer"
     misc_feature    complement(1959..1981)
                     /label="M13_pUC_fwd_primer"
     gene            complement(2489..3499)
                     /label="spect"
                     /gene="spect"                     
     CDS             complement(2489..3499)
                     /label="ORF frame 2"                     
     CDS             3429..3887
                     /label="ORF frame 3"                     
     CDS             complement(4314..5264)
                     /label="ORF frame 1"                     
     promoter        6135..6164
                     /label="lac_promoter"
                     
ORIGIN
    1 CCTGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC
   61 GGCGTAGAGG ATCTGCTCAT GTTTGACAGC TTATCATCGA TGCATAATGT GCCTGTCAAA
  121 TGGACGAAGC AGGGATTCTG CAAACCCTAT GCTACTCCGT CAAGCCGTCA ATTGTCTGAT
  181 TCGTTACCAA TTATGACAAC TTGACGGCTA CATCATTCAC TTTTTCTTCA CAACCGGCAC
  241 GGAACTCGCT CGGGCTGGCC CCGGTGCATT TTTTAAATAC CCGCGAGAAA TAGAGTTGAT
  301 CGTCAAAACC AACATTGCGA CCGACGGTGG CGATAGGCAT CCGGGTGGTG CTCAAAAGCA
  361 GCTTCGCCTG GCTGATACGT TGGTCCTCGC GCCAGCTTAA GACGCTAATC CCTAACTGCT
  421 GGCGGAAAAG ATGTGACAGA CGCGACGGCG ACAAGCAAAC ATGCTGTGCG ACGCTGGCGA
  481 TATCAAAATT GCTGTCTGCC AGGTGATCGC TGATGTACTG ACAAGCCTCG CGTACCCGAT
  541 TATCCATCGG TGGATGGAGC GACTCGTTAA TCGCTTCCAT GCGCCGCAGT AACAATTGCT
  601 CAAGCAGATT TATCGCCAGC AGCTCCGAAT AGCGCCCTTC CCCTTGCCCG GCGTTAATGA
  661 TTTGCCCAAA CAGGTCGCTG AAATGCGGCT GGTGCGCTTC ATCCGGGCGA AAGAACCCCG
  721 TATTGGCAAA TATTGACGGC CAGTTAAGCC ATTCATGCCA GTAGGCGCGC GGACGAAAGT
  781 AAACCCACTG GTGATACCAT TCGCGAGCCT CCGGATGACG ACCGTAGTGA TGAATCTCTC
  841 CTGGCGGGAA CAGCAAAATA TCACCCGGTC GGCAAACAAA TTCTCGTCCC TGATTTTTCA
  901 CCACCCCCTG ACCGCGAATG GTGAGATTGA GAATATAACC TTTCATTCCC AGCGGTCGGT
  961 CGATAAAAAA ATCGAGATAA CCGTTGGCCT CAATCGGCGT TAAACCCGCC ACCAGATGGG
 1021 CATTAAACGA GTATCCCGGC AGCAGGGGAT CATTTTGCGC TTCAGCCATA CTTTTCATAC
 1081 TCCCGCCATT CAGAGAAGAA ACCAATTGTC CATATTGCAT CAGACATTGC CGTCACTGCG
 1141 TCTTTTACTG GCTCTTCTCG CTAACCAAAC CGGTAACCCC GCTTATTAAA AGCATTCTGT
 1201 AACAAAGCGG GACCAAAGCC ATGACAAAAA CGCGTAACAA AAGTGTCTAT AATCACGGCA
 1261 GAAAAGTCCA CATTGATTAT TTGCACGGCG TCACACTTTG CTATGCCATA GCATTTTTAT
 1321 CCATAAGATT AGCGGATCCT ACCTGACGCT TTTTATCGCA ACTCTCTACT GTTTCTCCAT
 1381 ACCCGTTTTT TTGGGCTAGC AGGAGGAATT CACCATGGTA CCCGGGGATC CTCTAGAGTC
 1441 GACCTGCAGG CATGCAAGCT TGGCTGTTTT GGCGGATGAG AGAAGATTTT CAGCCTGATA
 1501 CAGATTAAAT CAGAACGCAG AAGCGGTCTG ATAAAACAGA ATTTGCCTGG CGGCAGTAGC
 1561 GCGGTGGTCC CACCTGACCC CATGCCGAAC TCAGAAGTGA AACGCCGTAG CGCCGATGGT
 1621 AGTGTGGGGT CTCCCCATGC GAGAGTAGGG AACTGCCAGG CATCAAATAA AACGAAAGGC
 1681 TCAGTCGAAA GACTGGGCCT TTCGTTTTAT CTGTTGTTTG TCGGTGAACG CTCTCCTGAG
 1741 TAGGACAAAT CCGCCGGGAG CGGATTTGAA CGTTGCGAAG CAACGGCCCG GAGGGTGGCG
 1801 GGCAGGACGC CCGCCATAAA CTGCCAGGCA TCAAATTAAG CAGAAGGCCA TCCTGACGGA
 1861 TGGCCTTTTT GCGTTTCTAC AAACTCTTTT GTTTATTTTT CTAAATACAT TCAAATATGT
 1921 ATCCGCTCAT GAGACAATAA CCCTAGCTTA CTGGCCGTCG TTTTACAACG TCGTGACTGG
 1981 GAAAACCCTG GCGTTACCCA ACTTAATCGC CTTGCAGCAC ATCCCCCTTT CGCCAGCTGG
 2041 CGTAATAGCG AAGAGGCCCG CACCGATCGC CCTTCCCAAC AGTTGCGCAG CCTGAATGGC
 2101 GAATGGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA
 2161 TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGCC CCGACACCCG
 2221 CCAACACCCG CTGACGAGCT TAGTAAAGCC CTCGCTAGAT TTTAATGCGG ATGTTGCGAT
 2281 TACTTCGCCA ACTATTGCGA TAACAAGAAA AAGCCAGCCT TTCATGATAT ATCTCCCAAT
 2341 TTGTGTAGGG CTTATTATGC ACGCTTAAAA ATAATAAAAG CAGACTTGAC CTGATAGTTT
 2401 GGCTGTGAGC AATTATGTGC TTAGTGCATC TAACGCTTGA GTTAAGCCGC GCCGCGAAGC
 2461 GGCGTCGGCT TGAACGAATT GTTAGACATT ATTTGCCGAC TACCTTGGTG ATCTCGCCTT
 2521 TCACGTAGTG GACAAATTCT TCCAACTGAT CTGCGCGCGA GGCCAAGCGA TCTTCTTCTT
 2581 GTCCAAGATA AGCCTGTCTA GCTTCACGTA TGACGGGCTG ATACTGGGCC GGCAGGCGCT
 2641 CCATTGCCCA GTCGGCAGCG ACCTCCTTCG GCGCGATTTT GCCGGTTACT GCGCTGTACC
 2701 AAATGCGGGA CAACGTAAGC ACTACATTTC GCTCATCGCC AGCCCAGTCG GGCGGCGAGT
 2761 TCCATAGCGT TAAGGTTTCA TTTAGCGCCT CAAATAGATC CTGTTCAGGA ACCGGATCAA
 2821 AGAGTTCCTC CGCCGCTGGA CCTACCAAGG CAACGCTATG TTCTCTTGCT TTTGTCAGCA
 2881 AGATAGCCAG ATCAATGTCG ATCGTGGCTG GCTCGAAGAT ACCTGCAAGA ATGTCATTGC
 2941 GCTGCCATTC TCCAAATTGC AGTTCGCGCT TAGCTGGATA ACGCCACGGA ATGATGTCGT
 3001 CGTGCACAAC AATGGTGACT TCTACAGCGC GGAGAATCTC GCTCTCTCCA GGGGAAGCCG
 3061 AAGTTTCCAA AAGGTCGTTG ATCAAAGCTC GCCGCGTTGT TTCATCAAGC CTTACGGTCA
 3121 CCGTAACCAG CAAATCAATA TCACTGTGTG GCTTCAGGCC GCCATCCACT GCGGAGCCGT
 3181 ACAAATGTAC GGCCAGCAAC GTCGGTTCGA GATGGCGCTC GATGACGCCA ACTACCTCTG
 3241 ATAGTTGAGT CGATACTTCG GCGATCACCG CTTCCCTCAT GATGTTTAAC TTTGTTTTAG
 3301 GGCGACTGCC CTGCTGCGTA ACATCGTTGC TGCTCCATAA CATCAAACAT CGACCCACGG
 3361 CGTAACGCGC TTGCTGCTTG GATGCCCGAG GCATAGACTG TACCCCAAAA AAACAGTCAT
 3421 AACAAGCCAT GAAAACCGCC ACTGCGCCGT TACCACCGCT GCGTTCGGTC AAGGTTCTGG
 3481 ACCAGTTGCG TGAGCGCATA CGCTACTTGC ATTACAGCTT ACGAACCGAA CAGGCTTATG
 3541 TCCACTGGGT TCGTGCCTTC ATCCGTTTCC ACGGTGTGCG TCACCCGGCA ACCTTGGGCA
 3601 GCAGCGAAGT CGAGGCATTT CTGTCCTGGC TGGCGAACGA GCGCAAGGTT TCGGTCTCCA
 3661 CGCATCGTCA GGCATTGGCG GCCTTGCTGT TCTTCTACGG CAAGGTGCTG TGCACGGATC
 3721 TGCCCTGGCT TCAGGAGATC GGAAGACCTC GGCCGTCGCG GCGCTTGCCG GTGGTGCTGA
 3781 CCCCGGATGA AGTAATTCCC ACGGGTTTTG CTGCCCGCAA ACGGGCTGTT CTGGTGTTGC
 3841 TAGTTTGTTA TCAGAATCGC AGATCCGGCT TCAGGTTTGC CGGCTGAAAG CGCTATTTCT
 3901 TCCAGAATTG CCATGATTTT TTCCCCACGG GAGGCGTCAC TGGCTCCCGT GTTGTCGGCA
 3961 GCTTTGATTC GATAAGCAGC ATCGCCTGTT TCAGGCTGTC TATGTGTGAC TGTTGAGCTG
 4021 TAACAAGTTG TCTCAGGTGT TCAATTTCAT GTTCTAGTTG CTTTGTTTTA CTGGTTTCAC
 4081 CTGTTCTATT AGGTGTTACA TGCTGTTCAT CTGTTACATT GTCGATCTGT TCATGGTGAA
 4141 CAGCTTTGAA TGCACCAAAA ACTCGTAAAA GCTCTGATGT ATCTATCTTT TTTACACCGT
 4201 TTTCATCTGT GCATATGGAC AGTTTTCCCT TTGATATGTA ACGGTGAACA GTTGTTCTAC
 4261 TTTTGTTTGT TAGTCTTGAT GCTTCACTGA TAGATACAAG AGCCATAAGA ACCTCAGATC
 4321 CTTCCGTATT TAGCCAGTAT GTTCTCTAGT GTGGTTCGTT GTTTTTGCGT GAGCCATGAG
 4381 AACGAACCAT TGAGATCATA CTTACTTTGC ATGTCACTCA AAAATTTTGC CTCAAAACTG
 4441 GTGAGCTGAA TTTTTGCAGT TAAAGCATCG TGTAGTGTTT TTCTTAGTCC GTTATGTAGG
 4501 TAGGAATCTG ATGTAATGGT TGTTGGTATT TTGTCACCAT TCATTTTTAT CTGGTTGTTC
 4561 TCAAGTTCGG TTACGAGATC CATTTGTCTA TCTAGTTCAA CTTGGAAAAT CAACGTATCA
 4621 GTCGGGCGGC CTCGCTTATC AACCACCAAT TTCATATTGC TGTAAGTGTT TAAATCTTTA
 4681 CTTATTGGTT TCAAAACCCA TTGGTTAAGC CTTTTAAACT CATGGTAGTT ATTTTCAAGC
 4741 ATTAACATGA ACTTAAATTC ATCAAGGCTA ATCTCTATAT TTGCCTTGTG AGTTTTCTTT
 4801 TGTGTTAGTT CTTTTAATAA CCACTCATAA ATCCTCATAG AGTATTTGTT TTCAAAAGAC
 4861 TTAACATGTT CCAGATTATA TTTTATGAAT TTTTTTAACT GGAAAAGATA AGGCAATATC
 4921 TCTTCACTAA AAACTAATTC TAATTTTTCG CTTGAGAACT TGGCATAGTT TGTCCACTGG
 4981 AAAATCTCAA AGCCTTTAAC CAAAGGATTC CTGATTTCCA CAGTTCTCGT CATCAGCTCT
 5041 CTGGTTGCTT TAGCTAATAC ACCATAAGCA TTTTCCCTAC TGATGTTCAT CATCTGAGCG
 5101 TATTGGTTAT AAGTGAACGA TACCGTCCGT TCTTTCCTTG TAGGGTTTTC AATCGTGGGG
 5161 TTGAGTAGTG CCACACAGCA TAAAATTAGC TTGGTTTCAT GCTCCGTTAA GTCATAGCGA
 5221 CTAATCGCTA GTTCATTTGC TTTGAAAACA ACTAATTCAG ACATACATCT CAATTGGTCT
 5281 AGGTGATTTT AATCACTATA CCAATTGAGA TGGGCTAGTC AATGATAATT ACTAGTCCTT
 5341 TTCCTTTGAG TTGTGGGTAT CTGTAAATTC TGCTAGACCT TTGCTGGAAA ACTTGTAAAT
 5401 TCTGCTAGAC CCTCTGTAAA TTCCGCTAGA CCTTTGTGTG TTTTTTTTGT TTATATTCAA
 5461 GTGGTTATAA TTTATAGAAT AAAGAAAGAA TAAAAAAAGA TAAAAAGAAT AGATCCCAGC
 5521 CCTGTGTATA ACTCACTACT TTAGTCAGTT CCGCAGTATT ACAAAAGGAT GTCGCAAACG
 5581 CTGTTTGCTC CTCTACAAAA CAGACCTTAA AACCCTAAAG GCTTAAGTAG CACCCTCGCA
 5641 AGCTCGGGCA AATCGCTGAA TATTCCTTTT GTCTCCGACC ATCAGGCACC TGAGTCGCTG
 5701 TCTTTTTCGT GACATTCAGT TCGCTGCGCT CACGGCTCTG GCAGTGAATG GGGGTAAATG
 5761 GCACTACAGG CGCCTTTTAT GGATTCATGC AAGGAAACTA CCCATAATAC AAGAAAAGCC
 5821 CGTCACGGGC TTCTCAGGGC GTTTTATGGC GGGTCTGCTA TGTGGTGCTA TCTGACTTTT
 5881 TGCTGTTCAG CAGTTCCTGC CCTCTGATTT TCCAGTCTGA CCACTTCGGA TTATCCCGTG
 5941 ACAGGTCATT CAGACTGGCT AATGCACCCA GTAAGGCAGC GGTATCATCA ACAGGCTTAC
 6001 CCGTCTTACT GTCGGGAATT AATTCGTTGG CCGATTCATT AATGCAGCTG GCACGACAGG
 6061 TTTCCCGACT GGAAAGCGGG CAGTGAGCGC AACGCAATTA ATGTGAGTTA GCTCACTCAT
 6121 TAGGCACCCC AGGCTTTACA CTTTATGCTT CCGGCTCGTA TGTTGTGTGG AATTGTGAG
//

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