pBAD18

价格:900元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Beckwith lab
载体名称: pBAD18
质粒类型: 大肠杆菌表达载体
高拷贝/低拷贝: --
启动子: --
克隆方法: 多克隆位点,限制性内切酶
载体大小: 4613 bp
5' 测序引物及序列: ctgtttctccatacccgtt
3' 测序引物及序列: --
载体标签: --
载体抗性: Ampicillin
筛选标记: --
备注: --
产品目录号: --
稳定性: --
组成型: 诱导型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pBAD18载体图谱



载体简介

This vector is one of several tightly controlled expression vectors (ATCC#8739387402)
regulated by the arabinose operon. Vectors differ in replicon, antibiotic resistance marker, 
multiple cloning site and need for translation initiation sequences. Cultures should be grown in 
minimal media for more reproducible induction of expression. Expression is induced in glycerolcontaining 
media by the addition of arabinose. 
Expression is repressed by addition of glucose or other catabolites. Cloned inserts must provide a translation 
initiation sequence (ATG) and ribosome binding site for expression. Expression of cloned inserts in the two
recommended hosts differ in terms of induction characteristics, especially when using low concentrations of
inducer (arabinose) and growing in minimal media. The following primers can be used for sequencing of
cloned inserts: 5 primer (278bp upstream of the NheI site) :5 CTGTTTCTCCATACCCGTT 3and one of two 3
primers:3 primer 1 (219bp downstream of the HindIII site):5 CTCATCCGCCAAAACAG 33 primer 2 (1733bp downstream of 
the HindIII site):5 GGCTGAAAATCTTCTCT3

载体序列


LOCUS       pBAD18	4613 bp 	DNA	SYN
DEFINITION  pBAD18
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..4613
                     /organism="pBAD18"
                     /mol_type="other DNA"
     misc_feature    complement(96..974)
                     /label="AraC"
     CDS             complement(96..1025)
                     /label="ORF frame 1"
                     /translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE
                     ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH
                     PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG
                     EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA
                     QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL
                     YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS*"
     misc_feature    1004..1019
                     /label="araO2"
                     /translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE
                     ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH
                     PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG
                     EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA
                     QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL
                     YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS*"
     misc_feature    1161..1172
                     /label="araO1"
                     /translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE
                     ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH
                     PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG
                     EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA
                     QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL
                     YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS*"
     misc_feature    1203..1216
                     /label="CAP_BS"
                     /translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE
                     ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH
                     PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG
                     EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA
                     QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL
                     YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS*"
     misc_feature    1208..1227
                     /label="pBAD_fwd_primer"
                     /translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE
                     ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH
                     PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG
                     EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA
                     QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL
                     YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS*"
     misc_feature    1213..1251
                     /label="AraI1I2"
                     /translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE
                     ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH
                     PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG
                     EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA
                     QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL
                     YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS*"
     promoter        1248..1276
                     /label="ARA_promoter"
                     /translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE
                     ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH
                     PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG
                     EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA
                     QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL
                     YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS*"
     misc_feature    complement(1395..1412)
                     /label="pTrcHis_rev_primer"
                     /translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE
                     ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH
                     PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG
                     EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA
                     QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL
                     YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS*"
     misc_feature    complement(1395..1412)
                     /label="pBAD_rev_primer"
                     /translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE
                     ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH
                     PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG
                     EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA
                     QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL
                     YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS*"
     terminator      1445..1602
                     /label="rrnB_terminator"
                     /translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE
                     ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH
                     PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG
                     EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA
                     QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL
                     YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS*"
     terminator      1568..1611
                     /label="rrnB_T1_terminator"
                     /translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE
                     ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH
                     PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG
                     EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA
                     QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL
                     YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS*"
     terminator      1743..1770
                     /label="rrnB_T2_terminator"
                     /translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE
                     ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH
                     PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG
                     EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA
                     QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL
                     YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS*"
     promoter        1811..1839
                     /label="AmpR_promoter"
                     /translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE
                     ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH
                     PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG
                     EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA
                     QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL
                     YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS*"
     gene            1881..2741
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE
                     ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH
                     PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG
                     EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA
                     QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL
                     YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS*"
     CDS             1881..2741
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      2800..3099
                     /label="f1_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      3333..3952
                     /label="pBR322_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    4349..4371
                     /label="pGEX_3_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    4510..4529
                     /label="pBRrevBam_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 atcgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac
   61 tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca
  121 ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta
  181 aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata
  241 ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag
  301 cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag
  361 caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg
  421 tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct
  481 tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc
  541 ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc
  601 gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca
  661 tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga
  721 tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa
  781 acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata
  841 taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc
  901 ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt
  961 tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat
 1021 tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta
 1081 accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt
 1141 aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca
 1201 ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta
 1261 tcgcaactct ctactgtttc tccatacccg tttttttggg ctagcgaatt cgagctcggt
 1321 acccggggat cctctagagt cgacctgcag gcatgcaagc ttggctgttt tggcggatga
 1381 gagaagattt tcagcctgat acagattaaa tcagaacgca gaagcggtct gataaaacag
 1441 aatttgcctg gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg
 1501 aaacgccgta gcgccgatgg tagtgtgggg tctccccatg cgagagtagg gaactgccag
 1561 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
 1621 gtcggtgaac gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgcgaa
 1681 gcaacggccc ggagggtggc gggcaggacg cccgccataa actgccaggc atcaaattaa
 1741 gcagaaggcc atcctgacgg atggcctttt tgcgtttcta caaactcttt tgtttatttt
 1801 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
 1861 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
 1921 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
 1981 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
 2041 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
 2101 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac
 2161 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
 2221 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
 2281 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
 2341 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
 2401 acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg
 2461 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
 2521 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
 2581 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
 2641 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
 2701 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
 2761 catatatact ttagattgat ttacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
 2821 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
 2881 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
 2941 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg
 3001 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
 3061 gagtccacgt tctttaatag tggactcttg ttccaaactt gaacaacact caaccctatc
 3121 tcgggctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
 3181 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttaa
 3241 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
 3301 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
 3361 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
 3421 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
 3481 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
 3541 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
 3601 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
 3661 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
 3721 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
 3781 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
 3841 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
 3901 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
 3961 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
 4021 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
 4081 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct
 4141 ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc
 4201 tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct
 4261 gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
 4321 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
 4381 tcatcaccga aacgcgcgag gcagcaagga gatggcgccc aacagtcccc cggccacggg
 4441 gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc
 4501 ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat
 4561 gccggccacg atgcgtccgg cgtagaggat ctgctcatgt ttgacagctt atc

//

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