pSH65

价格:1500元

联系方式:I47-825O-882O

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载体基本信息

出品公司: --
载体名称: pSH65
质粒类型: 酵母基因敲除载体
高拷贝/低拷贝: --
启动子: --
克隆方法: 多克隆位点,限制性内切酶
载体大小: 8138 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: --
载体抗性: Ampicillin
筛选标记: --
备注: --
产品目录号: --
稳定性: --
组成型: --
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pSH65载体图谱



载体简介

Plasmid is part of the " DEL-MARKER-SET ". For an individual plasmid order use accession number P30122.

Transformed E.coli cells are resistant to ampicillin. The plasmid contains the cre recombinase gene under control of a GAL promoter. Transformed Saccharomyces cerevisiae are resistent to phleomycin.

The pSH65 plasmid is usefull to express the cre recombinase under Galactose control. loxP flanked deletion markers will be removed from yeast deletions.

载体序列

LOCUS       pSH65   8138 bp 	DNA 	circular 	SYN 15-AUG-2011
DEFINITION  pSH65 Circularized
ACCESSION   pSH65
KEYWORDS .
SOURCE	    Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1 (bases 1 to 8138)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES               Location/Qualifiers
misc_feature           479..1286
                       /product="ura3 region"
CDS                    690..1286
                       /gene="delta-ura3"
                       /product="inactivated ura3 gene"
misc_feature           complement (2071..2274)
                       /gene="CYC1t"
                       /product="CYC1 terminator of S. cerevisiae"
CDS                    complement (2375..2755)
                       /gene="bleR"
                       /product=“ble gene from Tn5, conferring resistance to
                        bleomycin and phleomycin”;
misc_feature           complement (2774..3187)
                       /gene="ScTEF1p"
                       /product="TEF1 promoter of S. cerevisiae"
misc_feature           3231..3484
                       /gene="CYC1t"
                       /product="CYC1 terminator of S. cerevisiae"
CDS                    complement (3871..4832)
                       /gene="Cre"
                       /product="encoding of Cre recombinase"
CDS                    complement (4941..5408)
                       /gene="GAL1p"
                       /product="inducible GAL1 promoter, derived from the gene 
                        encoding galactokinase"
misc_feature           5851..5923
                       /gene="ori"
                       /product="ColE1 origin of replication"
CDS                    complement (6626..7486)
                       /gene="ampR"
                       /product="bla, encoding of beta-lactamase"
misc_feature           7627..1
                       /gene="CEN6/ARSH4"
                       /product="centromere sequence of chromosome VI (CEN6) plus 
                        the autonomously replicating sequence associated with histone 4 
                        (ARSH4)"
BASE   COUNT    2282 a    1805 c     1925 g     2126 t
ORIGIN
  1 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
 61 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
121 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
181 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
241 gcaccatacc acagcttttc aattcaattc atcatttttt ttttattctt ttttttgatt
301 tcggtttctt tgaaattttt ttgattcggt aatctccgaa cagaaggaag aacgaaggaa
361 ggagcacaga cttagattgg tatatatacg catatgtagt gttgaagaaa catgaaattg
421 cccagtattc ttaacccaac tgcacagaac aaaaacctgc aggaaacgaa gataaatcat
481 gtcgaaagct acatataagg aacgtgctgc tactcatcct agtcctgttg ctgccaagct
541 atttaatatc atgcacgaaa agcaaacaaa cttgtgtgct tcattggatg ttcgtaccac
601 caaggaatta ctggagttag ttgaagcatt aggtcccaaa atttgtttac taaaaacaca
661 tgtggatatc ttgactgatt tttccatgca tggagggcac agttaagccg ctaaaggcat
721 tatccgccaa gtacaatttt ttactcttcg aagacagaaa atttgctgac attggtaata
781 cagtcaaatt gcagtactct gcgggtgtat acagaatagc agaatgggca gacattacga
841 atgcacacgg tgtggtgggc ccaggtattg ttagcggttt gaagcaggcg gcagaagaag
901 taacaaagga acctagaggc cttttgatgt tagcagaatt gtcatgcaag ggctccctat
961 ctactggaga atatactaag ggtactgttg acattgcgaa gagcgacaaa gattttgtta
1021 tcggctttat tgctcaaaga gacatgggtg gaagagatga aggttacgat tggttgatta
1081 tgacacccgg tgtgggttta gatgacaagg gagacgcatt gggtcaacag tatagaaccg
1141 tggatgatgt ggtctctaca ggatctgaca ttattattgt tggaagagga ctatttgcaa
1201 agggaaggga tgctaaggta gagggtgaac gttacagaaa agcaggctgg gaagcatatt
1261 tgagaagatg cggccagcaa aactaaaaaa ctgtattata agtaaatgca tgtatactaa
1321 actcacaaat tagagcttca atttaattat atcagttatt accctatgcg gtgtgaaata
1381 ccgcacagat gcgtaaggag aaaataccgc atcaggaaat tgtaagcgtt aatattttgt
1441 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
1501 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
1561 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
1621 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
1681 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
1741 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
1801 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
1861 tacagggcgc gtcgcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
1921 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
1981 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg
2041 cgtaatacga ctcactatag ggcgaattgg gcgtacacgc gtctgtacag aaaaaaaaga
2101 aaaatttgaa atataaataa cgttcttaat actaacataa ctataaaaaa ataaataggg
2161 acctagactt caggttgtct aactccttcc ttttcggtta gagcggatgt ggggggaggg
2221 cgtgaatgta agcgtgacat aactaattac atgatatcga caaaggaaaa gggggacgga
2281 tccgtcgagt gggtggtgag cagctcgccg tcgcgcagca ggcgccagcg ctccatccac
2341 gcagtgacct caggcggaaa acgggaagac acactcatga gatgcctgca agcaattcgt
2401 tctgtatcag gcgcaggagc gtcccgtccg ggtcgaccaa agcggccatc gtgcctcccc
2461 actcctgcag ttcgggggca tggatgcgcg gatagccgct gctggtttcc tggatgccga
2521 cggatttgca ctgccggtag aactccgcga ggtcgtccag cctcaggcag cagctgaacc
2581 aactcgcgag gggatcgagc ccggggtggg cgaagaactc cagcatgaga tccccgcgct
2641 ggaggatcat ccagccggcg tcccggaaaa cgattccgaa gcccaacctt tcatagaagg
2701 cggcggtgga atcgaaatct cgtgatggca ggttgggcgt cgcttggtcg gtcatttcga
2761 attcgagctc gcccttagat tagattgcta tgctttcttt ctaatgagca agaagtaaaa
2821 aaagttgtaa tagaacaaga aaaatgaaac tgaaacttga gaaattgaag accgtttatt
2881 aacttaaata tcaatgggag gtcatcgaaa gagaaaaaaa tcaaaaaaaa aaattttcaa
2941 gaaaaagaaa cgtgataaaa atttttattg cctttttcga cgaagaaaaa gaaacgaggc
3001 ggtctctttt ttcttttcca aacctttagt acgggtaatt aacgacaccc tagaggaaga
3061 aagaggggaa atttagtatg ctgtgcttgg gtgttttgaa gtggtacggc gatgcgcgga
3121 gtccgagaaa atctggaaga gtaaaaaagg agtagaaaca ttttgaagct atggtgtgtg
3181 ggggatcccc gggtaccgag ctcgaattca ctggccgtcg ttttacaacg cggccgcaaa
3241 ttaaagcctt cgagcgtccc aaaaccttct caagcaaggt tttcagtata atgttacatg
3301 cgtacacgcg tctgtacaga aaaaaaagaa aaatttgaaa tataaataac gttcttaata
3361 ctaacataac tataaaaaaa taaataggga cctagacttc aggttgtcta actccttcct
3421 tttcggttag agcggatgtg gggggagggc gtgaatgtaa gcgtgacata actaattaca
3481 tgactcgagg tcgacaattc caaccttacc caagagttcg ccaaactcag acatcacttt
3541 agcaaaaccg cgccgtgctt cttcctcggt ggcattcatc acgaaatgtt cagcactacg
3601 catacttttg acaggaaacg caacggatat tgagtcaata tcaggcattc tatcgctcag
3661 ctttacagtg acaatgacgg ctggcgactg aatattagtg cttacagaca gcactacata
3721 ttttccgtcg atgttgaaat cctttctcat atgtcaccat aaatatcaaa taattatagc
3781 aatcatttac gcgttaatgg ctaatcgcca tcttccagca ggcgcaccat tgcccctgtt
3841 tcactatcca gggtacggat atagttcatg acaatattta cattggtcca gccaccagct
3901 tgcatgatct ccggtattga aactccagcg cgggccatat ctcgcgcggc tccgacacgg
3961 gcactgtgtc cagaccaggc caggtatctc tgaccagagt catccttagc gccgtaaatc
4021 aatcgatgag ttgcttcaaa aatcccttcc agggcgcgag ttgatagctg gctggtggca
4081 gatggcgcgg caacaccatt ttttctgacc cggcaaaaca ggtagttatt cggatcatca
4141 gctacaccag agacggaaat ccatcgctcg accagtttag ttacccccag gctaagtgcc
4201 ttctctacac ctgcggtgct aaccagcgtt ttcgttctgc caatatggat taacattctc
4261 ccaccgtcag tacgtgagat atctttaacc ctgatcctgg caatttcggc tatacgtaac
4321 agggtgttat aagcaatccc cagaaatgcc agattacgta tatcctggca gcgatcgcta
4381 ttttccatga gtgaacgaac ctggtcgaaa tcagtgcgtt cgaacgctag agcctgtttt
4441 gcacgttcac cggcatcaac gttttctttt cggatccgcc gcataaccag tgaaacagca
4501 ttgctgtcac ttggtcgtgg cagcccggac cgacgatgaa gcatgtttag ctggcccaaa
4561 tgttgctgga tagtttttac tgccagaccg cgcgcctgaa gatatagaag ataatcgcga
4621 acatcttcag gttctgcggg aaaccatttc cggttattca acttgcacca tgccgcccac
4681 gaccggcaaa cggacagaag cattttccag gtatgctcag aaaacgcctg gcgatccctg
4741 aacatgtcca tcaggttctt gcgaacctca tcactcgttg catcgaccgg taatgcaggc
4801 aaattttggt gtacggtcag taaattggac atttaacact cagataatgg ttttaagtaa
4861 agtgtacagg atcggctctg cccctcgacg gtatcgataa gcttgatatc gaattcctgc
4921 agcccggggg atccactagt tctagaatcc ggggtttttt ctccttgacg ttaaagtata
4981 gaggtatatt aacaattttt tgttgatact tttattacat ttgaataaga agtaatacaa
5041 accgaaaatg ttgaaagtat tagttaaagt ggttatgcag tttttgcatt tatatatctg
5101 ttaatagatc aaaaatcatc gcttcgctga ttaattaccc cagaaataag gctaaaaaac
5161 taatcgcatt atcatcctat ggttgttaat ttgattcgtt catttgaagg tttgtggggc
5221 caggttactg ccaatttttc ctcttcataa ccataaaagc tagtattgta gaatctttat
5281 tgttcggagc agtgcggcgc gaggcacatc tgcgtttcag gaacgcgacc ggtgaagacg
5341 aggacgcacg gaggagagtc ttccttcgga gggctgtcac ccgctcggcg gcttctaatc
5401 cgtactagag ctccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat
5461 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatag
5521 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgaggtaa ctcacattaa
5581 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
5641 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
5701 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
5761 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
5821 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
5881 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
5941 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
6001 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
6061 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
6121 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
6181 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
6241 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
6301 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
6361 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
6421 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
6481 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
6541 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
6601 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
6661 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
6721 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
6781 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
6841 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
6901 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
6961 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
7021 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
7081 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
7141 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
7201 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
7261 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
7321 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
7381 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
7441 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
7501 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
7561 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgggt
7621 ccttttcatc acgtgctata aaaataatta taatttaaat tttttaatat aaatatataa
7681 attaaaaata gaaagtaaaa aaagaaatta aagaaaaaat agtttttgtt ttccgaagat
7741 gtaaaagact ctagggggat cgccaacaaa tactaccttt tatcttgctc ttcctgctct
7801 caggtattaa tgccgaattg tttcatcttg tctgtgtaga agaccacaca cgaaaatcct
7861 gtgattttac attttactta tcgttaatcg aatgtatatc tatttaatct gcttttcttg
7921 tctaataaat atatatgtaa agtacgcttt ttgttgaaat tttttaaacc tttgtttatt
7981 tttttttctt cattccgtaa ctcttctacc ttctttattt actttctaaa atccaaatac
8041 aaaacataaa aataaataaa cacagagtaa attcccaaat tattccatca ttaaaagata
8101 cgaggcgcgt gtaagttaca ggcaagcgat ccgtccta
//

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