| 出品公司: | Clontech |
|---|---|
| 载体名称: | pLVX-DD-ZsGreen1 Control |
| 质粒类型: | 慢病毒载体；荧光报告载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | 无 |
| 载体大小: | 9134 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | DD tag (N-端） |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 嘌呤霉素（Puromycin） |
| 克隆菌株: | Stbl3 |
| 宿主细胞（系）: | 常规细胞系（293、CV-1、CHO等） |
| 备注: |
慢病毒载体pLVX-DD-ZsGreen1-Control组成型表达DD-ZsGreen1，是pLVX-DD-ZsGreen1 Reporter载体的阳性对照载体； DD-tdTomato与tdTomato相比，N端融合了destabilization domain (DD)去稳定结构域，导致ZsGreen1在细胞内很快被蛋白酶降解，降低了背景荧光，是研究启动子活性的理想报告基因。 |
| 产品目录号: | 631752 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 慢病毒 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLVX-DD-ZsGreen1_Control 9087 bp DNA circular 6-NOV-2009
COMMENT Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT Cat. No. 631751,631752
FEATURES Location/Qualifiers
LTR 1..635
/label=5'\LTR
misc_feature 685..822
/label=packaging\signal
promoter 2185..2787
/label=CMV\IE\Promoter
misc_feature 5055..5646
/label=WPRE
rep_origin complement(6955..7628)
/label=pUC\origin
CDS complement(7773..8769)
/label=Amp(R)
misc_binding 1303..1536
/label=RRE
misc_binding 636..653
/label=PBS
misc_feature 2028..2151
/label=cPPT/CTS
LTR 5849..6485
/label=3'\LTR
misc_feature 3913..4421
/label=PGK\Promoter
misc_feature 4442..5041
/label=Puro(R)
CDS 2880..3203
/label=DD
CDS 3210..3902
/label=ZsGreen1
BASE COUNT 2245 a 2363 c 2407 g 2072 t
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
2821 ctcaagcttc gaattctgca gtcgacggta ccgcgggccc gggatcccgc gactctagaa
2881 tgggagtgca ggtggaaacc atctccccag gagacgggcg caccttcccc aagcgcggcc
2941 agacctgtgt ggtgcactac accgggatgc ttgaagatgg aaagaaagtc gattcctccc
3001 gggacagaaa caagcccttt aagtttatgc taggcaagca ggaggtgatc cgaggctggg
3061 aagaaggggt tgcccagatg agtgtgggtc agagagccaa actgactata tctccagatt
3121 atgcctatgg tgccactggg cacccaggca tcatcccacc acatgccact ctcgtcttcg
3181 atgtggagct tctaaaaccg gaaagatcta tggcccagtc caagcacggc ctgaccaagg
3241 agatgaccat gaagtaccgc atggagggct gcgtggacgg ccacaagttc gtgatcaccg
3301 gcgagggcat cggctacccc ttcaagggca agcaggccat caacctgtgc gtggtggagg
3361 gcggcccctt gcccttcgcc gaggacatct tgtctgccgc cttcatgtac ggcaaccgcg
3421 tgttcaccga gtatccccag gacatcgtcg actacttcaa gaactcctgc cccgccggct
3481 acacctggga ccgctccttc ctgttcgagg acggcgccgt gtgcatctgc aacgccgaca
3541 tcaccgtgag cgtggaggag aactgcatgt accacgagtc caagttctac ggcgtgaact
3601 tccccgccga cggccccgtg atgaagaaga tgaccgacaa ctgggagccc tcctgcgaga
3661 agatcatccc cgtgcccaag cagggcatct tgaagggcga cgtgagcatg tacctgctgc
3721 tgaaggacgg tggccgcttg cgctgccagt tcgacaccgt gtacaaggcc aagtccgtgc
3781 cccgcaagat gcccgactgg cacttcatcc agcacaagct gacccgcgag gaccgcagcg
3841 acgccaagaa ccagaagtgg cacctgaccg agcacgccat cgcctccggc tccgccttgc
3901 cctagtctag ataattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat
3961 gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac
4021 attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac
4081 cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga
4141 cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc
4201 aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct
4261 gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg
4321 gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt
4381 ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcagc ccaagcttac
4441 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
4501 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
4561 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
4621 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
4681 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
4741 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
4801 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
4861 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
4921 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
4981 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
5041 accgcgtctg gaacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
5101 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
5161 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
5221 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
5281 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
5341 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
5401 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
5461 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
5521 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
5581 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
5641 cgcctggaat taattctgca gtcgagacct agaaaaacat ggagcaatca caagtagcaa
5701 tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg
5761 ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct
5821 tagccacttt ttaaaagaaa agaggggact ggaagggcta attcactccc aacgaagaca
5881 agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta
5941 cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc
6001 agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc
6061 tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag
6121 ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg
6181 atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg
6241 cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta
6301 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
6361 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
6421 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
6481 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
6541 cagagagtga gaggccttga cattgctagc gttttaccgt cgacctctag ctagagcttg
6601 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
6661 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
6721 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
6781 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
6841 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
6901 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
6961 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
7021 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
7081 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
7141 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
7201 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
7261 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
7321 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
7381 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
7441 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
7501 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
7561 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
7621 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
7681 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
7741 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
7801 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
7861 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
7921 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
7981 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
8041 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
8101 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
8161 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
8221 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
8281 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
8341 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
8401 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
8461 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
8521 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
8581 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
8641 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
8701 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
8761 cctgacgtcg acggatcggg agatcaactt gtttattgca gcttataatg gttacaaata
8821 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
8881 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa
8941 ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt
9001 tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg
9061 ttaaatatgt actacaaact tagtagt
//
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