pLVX-DD-ZsGreen1 Control

价格:3500元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Clontech
载体名称: pLVX-DD-ZsGreen1 Control
质粒类型: 慢病毒载体;荧光报告载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子:
载体大小: 9134 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: DD tag (N-端)
载体抗性: 氨苄青霉素
筛选标记: 嘌呤霉素(Puromycin)
克隆菌株: Stbl3
宿主细胞(系): 常规细胞系(293、CV-1、CHO等)
备注: 慢病毒载体pLVX-DD-ZsGreen1-Control组成型表达DD-ZsGreen1,是pLVX-DD-ZsGreen1 Reporter载体的阳性对照载体;
DD-tdTomato与tdTomato相比,N端融合了destabilization domain (DD)去稳定结构域,导致ZsGreen1在细胞内很快被蛋白酶降解,降低了背景荧光,是研究启动子活性的理想报告基因。
产品目录号: 631752
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 慢病毒

载体质粒图谱和多克隆位点信息

pLVX-DD-ZsGreen1 Control载体图谱



pLVX-DD-ZsGreen1 Control 载体特征

载体简介

载体序列

LOCUS       pLVX-DD-ZsGreen1_Control   9087 bp    DNA   circular             6-NOV-2009
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 631751,631752
FEATURES             Location/Qualifiers
     LTR             1..635
                     /label=5'\LTR
     misc_feature    685..822
                     /label=packaging\signal
     promoter        2185..2787
                     /label=CMV\IE\Promoter
     misc_feature    5055..5646
                     /label=WPRE
     rep_origin      complement(6955..7628)
                     /label=pUC\origin
     CDS             complement(7773..8769)
                     /label=Amp(R)
     misc_binding    1303..1536
                     /label=RRE
     misc_binding    636..653
                     /label=PBS
     misc_feature    2028..2151
                     /label=cPPT/CTS
     LTR             5849..6485
                     /label=3'\LTR
     misc_feature    3913..4421
                     /label=PGK\Promoter
     misc_feature    4442..5041
                     /label=Puro(R)
     CDS             2880..3203
                     /label=DD
     CDS             3210..3902
                     /label=ZsGreen1
BASE COUNT     2245 a      2363 c      2407 g      2072 t 
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 
     2821 ctcaagcttc gaattctgca gtcgacggta ccgcgggccc gggatcccgc gactctagaa 
     2881 tgggagtgca ggtggaaacc atctccccag gagacgggcg caccttcccc aagcgcggcc 
     2941 agacctgtgt ggtgcactac accgggatgc ttgaagatgg aaagaaagtc gattcctccc 
     3001 gggacagaaa caagcccttt aagtttatgc taggcaagca ggaggtgatc cgaggctggg 
     3061 aagaaggggt tgcccagatg agtgtgggtc agagagccaa actgactata tctccagatt 
     3121 atgcctatgg tgccactggg cacccaggca tcatcccacc acatgccact ctcgtcttcg 
     3181 atgtggagct tctaaaaccg gaaagatcta tggcccagtc caagcacggc ctgaccaagg 
     3241 agatgaccat gaagtaccgc atggagggct gcgtggacgg ccacaagttc gtgatcaccg 
     3301 gcgagggcat cggctacccc ttcaagggca agcaggccat caacctgtgc gtggtggagg 
     3361 gcggcccctt gcccttcgcc gaggacatct tgtctgccgc cttcatgtac ggcaaccgcg 
     3421 tgttcaccga gtatccccag gacatcgtcg actacttcaa gaactcctgc cccgccggct 
     3481 acacctggga ccgctccttc ctgttcgagg acggcgccgt gtgcatctgc aacgccgaca 
     3541 tcaccgtgag cgtggaggag aactgcatgt accacgagtc caagttctac ggcgtgaact 
     3601 tccccgccga cggccccgtg atgaagaaga tgaccgacaa ctgggagccc tcctgcgaga 
     3661 agatcatccc cgtgcccaag cagggcatct tgaagggcga cgtgagcatg tacctgctgc 
     3721 tgaaggacgg tggccgcttg cgctgccagt tcgacaccgt gtacaaggcc aagtccgtgc 
     3781 cccgcaagat gcccgactgg cacttcatcc agcacaagct gacccgcgag gaccgcagcg 
     3841 acgccaagaa ccagaagtgg cacctgaccg agcacgccat cgcctccggc tccgccttgc 
     3901 cctagtctag ataattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat 
     3961 gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac 
     4021 attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac 
     4081 cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga 
     4141 cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc 
     4201 aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct 
     4261 gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg 
     4321 gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt 
     4381 ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcagc ccaagcttac 
     4441 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt 
     4501 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga 
     4561 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga 
     4621 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga 
     4681 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg 
     4741 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga 
     4801 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg 
     4861 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct 
     4921 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc 
     4981 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg 
     5041 accgcgtctg gaacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc 
     5101 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg 
     5161 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc 
     5221 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg 
     5281 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg 
     5341 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga 
     5401 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct 
     5461 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg 
     5521 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc 
     5581 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc 
     5641 cgcctggaat taattctgca gtcgagacct agaaaaacat ggagcaatca caagtagcaa 
     5701 tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg 
     5761 ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct 
     5821 tagccacttt ttaaaagaaa agaggggact ggaagggcta attcactccc aacgaagaca 
     5881 agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta 
     5941 cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc 
     6001 agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc 
     6061 tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag 
     6121 ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg 
     6181 atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg 
     6241 cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta 
     6301 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc 
     6361 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt 
     6421 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta 
     6481 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat 
     6541 cagagagtga gaggccttga cattgctagc gttttaccgt cgacctctag ctagagcttg 
     6601 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac 
     6661 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc 
     6721 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg 
     6781 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct 
     6841 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 
     6901 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 
     6961 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 
     7021 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 
     7081 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 
     7141 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 
     7201 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 
     7261 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 
     7321 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 
     7381 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 
     7441 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 
     7501 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 
     7561 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 
     7621 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 
     7681 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 
     7741 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 
     7801 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 
     7861 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 
     7921 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 
     7981 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 
     8041 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg 
     8101 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 
     8161 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 
     8221 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 
     8281 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 
     8341 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat 
     8401 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 
     8461 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 
     8521 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 
     8581 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 
     8641 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 
     8701 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 
     8761 cctgacgtcg acggatcggg agatcaactt gtttattgca gcttataatg gttacaaata 
     8821 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 
     8881 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa 
     8941 ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt 
     9001 tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg 
     9061 ttaaatatgt actacaaact tagtagt 
//

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