| 质粒名称: | pPPEM |
|---|---|
| 目录编号: | 162467 |
| 存储实验室: | Adam Bogdanove |
|
载体骨架基本信息 |
|
| 载体名称: | pCAMBIA1300 |
| 空载体大小: | 8959 bp |
| 完整质粒大小: | 18478bp |
| 载体类型: | Plant Expression |
| 筛选标记: | Hygromycin |
| 载体抗性: | Kanamycin, 50 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 低拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Cas9H840A |
| 物种来源: | Synthetic |
| 插入大小: | 10576 bp |
| 基因突变: | Change histidine 840 to Alanine |
| GenBank编号 | JN247409 |
| 启动子: | Ubi |
| 标签/融合蛋白: | MLV reverse transcriptase (C terminal on insert) |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | Acc65I(未破坏) |
| 3’克隆位点: | SpeI(未破坏) |
| 5’测序引物 | ATCCGATCCAAATCCGAA |
| 3’测序引物 | TTCGAATTCCGAACCGTC |
|
文献引用方法 |
|
| 材料和方法部分: | pPPEM was a gift from Adam Bogdanove (Addgene plasmid # 162467 ; http://n2t.net/addgene:162467 ; RRID:Addgene_162467) |
| 参考文献部分: | Spelling Changes and Fluorescent Tagging With Prime Editing Vectors for Plants. Wang L, Kaya HB, Zhang N, Rai R, Willmann MR, Carpenter SCD, Read AC, Martin F, Fei Z, Leach JE, Martin GB, Bogdanove AJ. Front Genome Ed. 2021 Mar 4;3:617553. doi: 10.3389/fgeed.2021.617553. eCollection 2021. 10.3389/fgeed.2021.617553 PubMed 34713247 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pPPEM 19814 bp ds-DNA circular SYN 15-DEC-2021
DEFINITION Destination Expression vector for monocot plant primer editing.
ACCESSION .
VERSION .
KEYWORDS pPPEM
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 19814)
AUTHORS Wang L, Kaya HB, Zhang N, Rai R, Willmann MR, Carpenter SCD, Read
AC, Martin F, Fei Z, Leach JE, Martin GB, Bogdanove AJ
TITLE Spelling changes and fluorescent tagging with prime editing vectors
for plants.
JOURNAL Front. Genome Ed.
REFERENCE 2 (bases 1 to 19814)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Dec 15, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..19814
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(45..61)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(45..61)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(58..80)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 69..85
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(93..123)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 138..159
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 350..1026
/label=CaMV 35S promoter (enhanced)
/note="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
primer_bind 963..983
/label=35S promoter
/note="CaMV 35S promoter, forward primer"
CDS 1093..2118
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
K"
polyA_signal 2159..2333
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
misc_feature 2411..2435
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
CDS 2860..3654
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
rep_origin 5079..5667
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5568..5587
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 5821..5838
/label=L4440
/note="L4440 vector, forward primer"
misc_feature 5853..5993
/label=bom
/note="basis of mobility region from pBR322"
primer_bind complement(5919..5938)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 6079..6101
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
rep_origin 6337..6531
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
CDS complement(6597..7670)
/codon_start=1
/product="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 RepA
/translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
LVMRYRNLIEGEASAGS"
CDS complement(8099..8728)
/codon_start=1
/product="stability protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 StaA
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
misc_feature 10029..10053
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
primer_bind 10241..10263
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 10255..10272
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 10256..10272
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 10294..10418
/gene="mutant version of attR"
/label=attR1
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
promoter 10443..10473
/label=lac UV5 promoter
/note="E. coli lac promoter with an ""up"" mutation"
CDS 10829..11134
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/label=ccdB
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
primer_bind 11013..11032
/label=ccdB-fwd
/note="ccdB gene, forward primer"
protein_bind complement(11175..11299)
/gene="mutant version of attR"
/label=attR2
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
CDS 13147..13212
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
CDS 13219..13239
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 13264..17364
/codon_start=1
/product="nickase mutant of the Cas9 endonuclease from the
Streptococcus pyogenes Type II CRISPR/Cas system"
/label=Cas9(H840A)
/note="generates RNA-guided single strand nicks in DNA due
to the H840A mutation in the HNH catalytic domain"
/translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS 19540..19560
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
terminator 19586..24
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
ORIGIN
1 cggtgtcatc tatgttacta gatcgggaat taattcgtaa tcatgtcata gctgtttcct
61 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
121 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
181 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
241 agaggcggtt tgcgtattgg ctagagcagc ttgccaacat ggtggagcac gacactctcg
301 tctactccaa gaatatcaaa gatacagtct cagaagacca aagggctatt gagacttttc
361 aacaaagggt aatatcggga aacctcctcg gattccattg cccagctatc tgtcacttca
421 tcaaaaggac agtagaaaag gaaggtggca cctacaaatg ccatcattgc gataaaggaa
481 aggctatcgt tcaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccacga
541 ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg
601 aacatggtgg agcacgacac tctcgtctac tccaagaata tcaaagatac agtctcagaa
661 gaccaaaggg ctattgagac ttttcaacaa agggtaatat cgggaaacct cctcggattc
721 cattgcccag ctatctgtca cttcatcaaa aggacagtag aaaaggaagg tggcacctac
781 aaatgccatc attgcgataa aggaaaggct atcgttcaag atgcctctgc cgacagtggt
841 cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg
901 tcttcaaagc aagtggattg atgtgatatc tccactgacg taagggatga cgcacaatcc
961 cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt ggagaggaca
1021 cgctgaaatc accagtctct ctctacaaat ctatctctct cgagctttcg cagatccggg
1081 gggcaatgag atatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc
1141 gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga atctcgtgct
1201 ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg cgccgatggt
1261 ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc gattccggaa
1321 gtgcttgaca ttggggagtt tagcgagagc ctgacctatt gcatctcccg ccgttcacag
1381 ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctaca accggtcgcg
1441 gaggctatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt cggcccattc
1501 ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc gattgctgat
1561 ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag
1621 gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca cctcgtgcac
1681 gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc ggtcattgac
1741 tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt cttctggagg
1801 ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca tccggagctt
1861 gcaggatcgc cacgactccg ggcgtatatg ctccgcattg gtcttgacca actctatcag
1921 agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc
1981 gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc
2041 tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc cagcactcgt
2101 ccgagggcaa agaaatagag tagatgccga ccgggatctg tcgatcgaca agctcgagtt
2161 tctccataat aatgtgtgag tagttcccag ataagggaat tagggttcct atagggtttc
2221 gctcatgtgt tgagcatata agaaaccctt agtatgtatt tgtatttgta aaatacttct
2281 atcaataaaa tttctaattc ctaaaaccaa aatccagtac taaaatccag atcccccgaa
2341 ttaattcggc gttaattcag tacattaaaa acgtccgcaa tgtgttatta agttgtctaa
2401 gcgtcaattt gtttacacca caatatatcc tgccaccagc cagccaacag ctccccgacc
2461 ggcagctcgg cacaaaatca ccactcgata caggcagccc atcagtccgg gacggcgtca
2521 gcgggagagc cgttgtaagg cggcagactt tgctcatgtt accgatgcta ttcggaagaa
2581 cggcaactaa gctgccgggt ttgaaacacg gatgatctcg cggagggtag catgttgatt
2641 gtaacgatga cagagcgttg ctgcctgtga tcaccgcggt ttcaaaatcg gctccgtcga
2701 tactatgtta tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag
2761 gttttagaat gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg
2821 tatctttaaa tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca
2881 ccggaattga aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct
2941 cctgctaagg tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac
3001 agccggtata aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg
3061 gaaggaaagc tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat
3121 ctgctcatga gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc
3181 cctgaaaaga ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata
3241 tcggattgtc cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg
3301 aataacgatc tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat
3361 ccgcgcgagc tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc
3421 cacggcgacc tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt
3481 gatcttggga gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg
3541 atcagggagg atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc
3601 aagcctgatt gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta
3661 gaatgcatga ccaaaatccc ttaacgctga gagatcccct cataatttcc ccaaagcgta
3721 accatgtgtg aataaatttt gagctagtag ggttgcagcc acgagtaagt cttcccttgt
3781 tattgtgtag ccagaatgcc gcaaaacttc catgcctaag cgaactgttg agagtacgtt
3841 tcgatttctg actgtgttag cctggaagtg cttgtcccaa ccttgtttct gagcatgaac
3901 gcccgcaagc caacatgtta gttgaagcat cagggcgatt agcagcatga tatcaaaacg
3961 ctctgagctg ctcgttcggc tatggcgtag gcctagtccg taggcaggac ttttcaagtc
4021 tcggaaggtt tcttcaatct gcattcgctt cgaatagata ttaacaagtt gtttgggtgt
4081 tcgaatttca acaggtaagt tagttgctag aatccatggc tcctttgccg acgctgagta
4141 gattttaggt gacgggtggt gacaatgagt ccgtgtcgag cgctgatttt ttcggccttt
4201 agagcgagat ttatacaata gaatttggca tgagattgga ttgcttttag tcagcctctt
4261 atagcctaaa gtctttgagt gactagatga catatcatgt aagttgctga taggtttcca
4321 gttttccgct cctaggtctg catattgtac ttttcctctt actcgactta accagtacca
4381 acccagcttc tcaacggatt tataccatgg cactttaaag ccagcatcac tgacaatgag
4441 cggtgtggtg ttactcggta gaatgctcgc aaggtcggct agaaattggt catgagcttt
4501 ctttgaacat tgctctgaaa gcgggaacgc tttctcataa agagtaacag aacgaccgtg
4561 tagtgcgact gaagctcgca ataccataag ccgtttttgc tcacggatat cagaccagtc
4621 aacaagtaca atgggcatcg tattgcccga acagataaag ctagcatgcc aacggtatac
4681 agcgagtcgc tctttgtgga ggtgacgatt acctaacaat cggtcgattc gtttgatgtt
4741 atgttttgtt ctcgctttgg ttggcaggtt acggccaagt tcggtaagag tgagagtttt
4801 acagtcaagt aaggcgtggc aagccaacgt taagctgttg agtcgtttta agtgtaattc
4861 ggggcagaat tggtaaagag agtcgtgtaa aatatcgagt tcgcacattt tgttgtctga
4921 ttattgattt ttggcgaaac catttgatca tatgacaaga tgtgtatcta ccttaactta
4981 atgattttga taaaaatcat taggggattc atcagccctt aacgtgagtt ttcgttccac
5041 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
5101 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
5161 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
5221 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
5281 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
5341 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
5401 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
5461 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
5521 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
5581 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
5641 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
5701 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
5761 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
5821 agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat
5881 ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca
5941 tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac
6001 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
6061 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
6121 aacgcgcgag gcagggtgcc ttgatgtggg cgccggcggt cgagtggcga cggcgcggct
6181 tgtccgcgcc ctggtagatt gcctggccgt aggccagcca tttttgagcg gccagcggcc
6241 gcgataggcc gacgcgaagc ggcggggcgt agggagcgca gcgaccgaag ggtaggcgct
6301 ttttgcagct cttcggctgt gcgctggcca gacagttatg cacaggccag gcgggtttta
6361 agagttttaa taagttttaa agagttttag gcggaaaaat cgcctttttt ctcttttata
6421 tcagtcactt acatgtgtga ccggttccca atgtacggct ttgggttccc aatgtacggg
6481 ttccggttcc caatgtacgg ctttgggttc ccaatgtacg tgctatccac aggaaagaga
6541 ccttttcgac ctttttcccc tgctagggca atttgcccta gcatctgctc cgtacattag
6601 gaaccggcgg atgcttcgcc ctcgatcagg ttgcggtagc gcatgactag gatcgggcca
6661 gcctgccccg cctcctcctt caaatcgtac tccggcaggt catttgaccc gatcagcttg
6721 cgcacggtga aacagaactt cttgaactct ccggcgctgc cactgcgttc gtagatcgtc
6781 ttgaacaacc atctggcttc tgccttgcct gcggcgcggc gtgccaggcg gtagagaaaa
6841 cggccgatgc cgggatcgat caaaaagtaa tcggggtgaa ccgtcagcac gtccgggttc
6901 ttgccttctg tgatctcgcg gtacatccaa tcagctagct cgatctcgat gtactccggc
6961 cgcccggttt cgctctttac gatcttgtag cggctaatca aggcttcacc ctcggatacc
7021 gtcaccaggc ggccgttctt ggccttcttc gtacgctgca tggcaacgtg cgtggtgttt
7081 aaccgaatgc aggtttctac caggtcgtct ttctgctttc cgccatcggc tcgccggcag
7141 aacttgagta cgtccgcaac gtgtggacgg aacacgcggc cgggcttgtc tcccttccct
7201 tcccggtatc ggttcatgga ttcggttaga tgggaaaccg ccatcagtac caggtcgtaa
7261 tcccacacac tggccatgcc ggccggccct gcggaaacct ctacgtgccc gtctggaagc
7321 tcgtagcgga tcacctcgcc agctcgtcgg tcacgcttcg acagacggaa aacggccacg
7381 tccatgatgc tgcgactatc gcgggtgccc acgtcataga gcatcggaac gaaaaaatct
7441 ggttgctcgt cgcccttggg cggcttccta atcgacggcg caccggctgc cggcggttgc
7501 cgggattctt tgcggattcg atcagcggcc gcttgccacg attcaccggg gcgtgcttct
7561 gcctcgatgc gttgccgctg ggcggcctgc gcggccttca acttctccac caggtcatca
7621 cccagcgccg cgccgatttg taccgggccg gatggtttgc gaccgctcac gccgattcct
7681 cgggcttggg ggttccagtg ccattgcagg gccggcaggc aacccagccg cttacgcctg
7741 gccaaccgcc cgttcctcca cacatggggc attccacggc gtcggtgcct ggttgttctt
7801 gattttccat gccgcctcct ttagccgcta aaattcatct actcatttat tcatttgctc
7861 atttactctg gtagctgcgc gatgtattca gatagcagct cggtaatggt cttgccttgg
7921 cgtaccgcgt acatcttcag cttggtgtga tcctccgccg gcaactgaaa gttgacccgc
7981 ttcatggctg gcgtgtctgc caggctggcc aacgttgcag ccttgctgct gcgtgcgctc
8041 ggacggccgg cacttagcgt gtttgtgctt ttgctcattt tctctttacc tcattaactc
8101 aaatgagttt tgatttaatt tcagcggcca gcgcctggac ctcgcgggca gcgtcgccct
8161 cgggttctga ttcaagaacg gttgtgccgg cggcggcagt gcctgggtag ctcacgcgct
8221 gcgtgatacg ggactcaaga atgggcagct cgtacccggc cagcgcctcg gcaacctcac
8281 cgccgatgcg cgtgcctttg atcgcccgcg acacgacaaa ggccgcttgt agccttccat
8341 ccgtgacctc aatgcgctgc ttaaccagct ccaccaggtc ggcggtggcc catatgtcgt
8401 aagggcttgg ctgcaccgga atcagcacga agtcggctgc cttgatcgcg gacacagcca
8461 agtccgccgc ctggggcgct ccgtcgatca ctacgaagtc gcgccggccg atggccttca
8521 cgtcgcggtc aatcgtcggg cggtcgatgc cgacaacggt tagcggttga tcttcccgca
8581 cggccgccca atcgcgggca ctgccctggg gatcggaatc gactaacaga acatcggccc
8641 cggcgagttg cagggcgcgg gctagatggg ttgcgatggt cgtcttgcct gacccgcctt
8701 tctggttaag tacagcgata accttcatgc gttccccttg cgtatttgtt tatttactca
8761 tcgcatcata tacgcagcga ccgcatgacg caagctgttt tactcaaata cacatcacct
8821 ttttagacgg cggcgctcgg tttcttcagc ggccaagctg gccggccagg ccgccagctt
8881 ggcatcagac aaaccggcca ggatttcatg cagccgcacg gttgagacgt gcgcgggcgg
8941 ctcgaacacg tacccggccg cgatcatctc cgcctcgatc tcttcggtaa tgaaaaacgg
9001 ttcgtcctgg ccgtcctggt gcggtttcat gcttgttcct cttggcgttc attctcggcg
9061 gccgccaggg cgtcggcctc ggtcaatgcg tcctcacgga aggcaccgcg ccgcctggcc
9121 tcggtgggcg tcacttcctc gctgcgctca agtgcgcggt acagggtcga gcgatgcacg
9181 ccaagcagtg cagccgcctc tttcacggtg cggccttcct ggtcgatcag ctcgcgggcg
9241 tgcgcgatct gtgccggggt gagggtaggg cgggggccaa acttcacgcc tcgggccttg
9301 gcggcctcgc gcccgctccg ggtgcggtcg atgattaggg aacgctcgaa ctcggcaatg
9361 ccggcgaaca cggtcaacac catgcggccg gccggcgtgg tggtgtcggc ccacggctct
9421 gccaggctac gcaggcccgc gccggcctcc tggatgcgct cggcaatgtc cagtaggtcg
9481 cgggtgctgc gggccaggcg gtctagcctg gtcactgtca caacgtcgcc agggcgtagg
9541 tggtcaagca tcctggccag ctccgggcgg tcgcgcctgg tgccggtgat cttctcggaa
9601 aacagcttgg tgcagccggc cgcgtgcagt tcggcccgtt ggttggtcaa gtcctggtcg
9661 tcggtgctga cgcgggcata gcccagcagg ccagcggcgg cgctcttgtt catggcgtaa
9721 tgtctccggt tctagtcgca agtattctac tttatgcgac taaaacacgc gacaagaaaa
9781 cgccaggaaa agggcagggc ggcagcctgt cgcgtaactt aggacttgtg cgacatgtcg
9841 ttttcagaag acggctgcac tgaacgtcag aagccgactg cactatagca gcggaggggt
9901 tggatcaaag tactttgatc ccgaggggaa ccctgtggtt ggcatgcaca tacaaatgga
9961 cgaacggata aaccttttca cgccctttta aatatccgat tattctaata aacgctcttt
10021 tctcttaggt ttacccgcca atatatcctg tcaaacactg atagtttaaa ctgaaggcgg
10081 gaaacgacaa tctgatccaa gctcaagctg ctctagcatt cgccattcag gctgcgcaac
10141 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
10201 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
10261 acgacggcca gtgccatgct agagacgggg atcacaagtt tgtacaaaaa agctgaacga
10321 gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact
10381 acataatact gtaaaacaca acatatccag tcactatggc ggccgcatta ggcaccccag
10441 gctttacact ttatgcttcc ggctcgtata atgtgtggat tttgagttag gatcgatccg
10501 gcttactaaa agccagataa cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa
10561 tatatactga tatgtatacc cgaagtatgt caaaaagagg tatgctatga agcagcgtat
10621 tacagtgaca gttgacagcg acagctatca gttgctcaag gcatatatga tgtcaatatc
10681 tccggtctgg taagcacaac catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg
10741 aaagcggaaa atcaggaagg gatggctgag gtcgcccggt ttattgaaat gaacggctct
10801 tttgctgacg agaacagggg ctggtgaaat gcagtttaag gtttacacct ataaaagaga
10861 gagccgttat cgtctgtttg tggatgtaca gagtgatatt attgacacgc ccgggcgacg
10921 gatggtgatc cccctggcca gtgcacgtct gctgtcagat aaagtctccc gtgaacttta
10981 cccggtggtg catatcgggg atgaaagctg gcgcatgatg accaccgata tggccagtgt
11041 gccggtctcc gttatcgggg aagaagtggc tgatctcagc caccgcgaaa atgacatcaa
11101 aaacgccatt aacctgatgt tctggggaat ataaatgtca ggctccctta tacacagcca
11161 gtctgcaggt cgaccatagt gactggatat gttgtgtttt acagtattat gtagtctgtt
11221 ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt tctcgttcag
11281 ctttcttgta caaagtggtg aagcttgcat gcctgcagtg cagcgtgacc cggtcgtgcc
11341 cctctctaga gataatgagc attgcatgtc taagttataa aaaattacca catatttttt
11401 ttgtcacact tgtttgaagt gcagtttatc tatctttata catatattta aactttactc
11461 tacgaataat ataatctata gtactacaat aatatcagtg ttttagagaa tcatataaat
11521 gaacagttag acatggtcta aaggacaatt gagtattttg acaacaggac tctacagttt
11581 tatcttttta gtgtgcatgt gttctccttt ttttttgcaa atagcttcac ctatataata
11641 cttcatccat tttattagta catccattta gggtttaggg ttaatggttt ttatagacta
11701 atttttttag tacatctatt ttattctatt ttagcctcta aattaagaaa actaaaactc
11761 tattttagtt tttttattta ataatttaga tataaaatag aataaaataa agtgactaaa
11821 aattaaacaa atacccttta agaaattaaa aaaactaagg aaacattttt cttgtttcga
11881 gtagataatg ccagcctgtt aaacgccgtc gacgagtcta acggacacca accagcgaac
11941 cagcagcgtc gcgtcgggcc aagcgaagca gacggcacgg catctctgtc gctgcctctg
12001 gacccctctc gagagttccg ctccaccgtt ggacttgctc cgctgtcggc atccagaaat
12061 tgcgtggcgg agcggcagac gtgagccggc acggcaggcg gcctcctcct cctctcacgg
12121 caccggcagc tacgggggat tcctttccca ccgctccttc gctttccctt cctcgcccgc
12181 cgtaataaat agacaccccc tccacaccct ctttccccaa cctcgtgttg ttcggagcgc
12241 acacacacac aaccagatct cccccaaatc cacccgtcgg cacctccgct tcaaggtacg
12301 ccgctcgtcc tccccccccc ctctctacct tctctagatc ggcgttccgg tccatggtta
12361 gggcccggta gttctacttc tgttcatgtt tgtgttagat ccgtgtttgt gttagatccg
12421 tgctgctagc gttcgtacac ggatgcgacc tgtacgtcag acacgttctg attgctaact
12481 tgccagtgtt tctctttggg gaatcctggg atggctctag ccgttccgca gacgggatcg
12541 atttcatgat tttttttgtt tcgttgcata gggtttggtt tgcccttttc ctttatttca
12601 atatatgccg tgcacttgtt tgtcgggtca tcttttcatg cttttttttg tcttggttgt
12661 gatgatgtgg tgtggttggg cggtcgttca ttcgttctag atcggagtag aatactgttt
12721 caaactacct ggtgtattta ttaattttgg aactgtatgt gtgtgtcata catcttcata
12781 gttacgagtt taagatggat ggaaatatcg atctaggata ggtatacatg ttgatgtggg
12841 ttttactgat gcatatacat gatggcatat gcagcatcta ttcatatgct ctaaccttga
12901 gtacctatct attataataa acaagtatgt tttataatta ttttgatctt gatatacttg
12961 gatgatggca tatgcagcag ctatatgtgg atttttttag ccctgccttc atacgctatt
13021 tatttgcttg gtactgtttc ttttgtcgat gctcaccctg ttgtttggtg ttacttctgc
13081 aggtcgactc tagaggatct ggaattcccg ggtaccggat ccgatccaaa tccgaatccg
13141 gccatggact ataaggatca cgatggcgac tacaaggatc atgacattga ctataaggat
13201 gacgacgata agatggcacc taagaagaaa aggaaagtcg gcattcatgg cgttccggca
13261 gccgacaaaa agtatagcat cggcctcgat attgggacaa actctgtggg ctgggcggta
13321 attaccgacg agtacaaggt gcctagtaag aaatttaaag tgctcggaaa cactgacagg
13381 cactctataa agaagaacct gatcggggca ctgcttttcg actccggaga gacggcggag
13441 gcgacgcgtc tcaagcgtac cgcgcgccgc aggtacacaa gaaggaagaa taggatctgc
13501 tacttgcagg aaatcttcag taacgagatg gcgaaggtcg acgatagttt ctttcatcgg
13561 ttggaagaat cgttcctcgt agaggaggac aaaaagcacg agcgtcaccc aatattcggg
13621 aatattgttg acgaggttgc ctaccatgag aaatatccta caatatatca cctccgtaag
13681 aagcttgtcg attcaactga taaggctgat ctcagactca tctatcttgc cctcgcacat
13741 atgattaagt ttcgtggcca cttcttgatt gaaggcgacc tcaacccgga caactcagat
13801 gttgacaagc tttttataca gctcgtccag acatataacc agctgtttga agagaatccc
13861 atcaatgcga gtggggttga tgctaaggcc attttgtccg ccaggttgtc caaatctcgc
13921 agactggaaa acctgatcgc acagcttccc ggtgaaaaga aaaacgggct cttcggcaat
13981 ctcatcgcac tgtccctcgg cctcacccca aacttcaagt ctaacttcga cctggccgag
14041 gatgcgaagc tccagctgtc aaaagataca tacgacgacg atttggacaa tctgcttgcg
14101 caaataggcg accagtatgc ggacctgttc ctggctgcca aaaatctgtc agatgcaatc
14161 ctcctgtccg atatattgcg tgtgaacacc gaaatcacga aggcaccgct tagcgcatcc
14221 atgatcaaga gatacgacga gcaccatcag gacctcacac tcctcaaggc gcttgttcgt
14281 cagcagcttc ccgagaaata taaggaaatt tttttcgatc aaagcaagaa tggatatgct
14341 ggctatattg acggtggcgc ttcgcaggag gagttctata aattcattaa gccgattctg
14401 gagaagatgg acggaacgga ggagctcctc gtcaagctta accgggaaga cctgttgcgg
14461 aagcagagga cttttgataa cggctctatt ccgcaccaaa tccatctggg tgagttgcac
14521 gcaatcttga gaagacaaga ggatttctac ccgttcctta aggataacag agagaagata
14581 gaaaaaatac tgaccttcag gataccatac tatgtgggcc cactggcgcg cggaaatagt
14641 cgtttcgcat ggatgactag aaagtccgaa gaaacgatca cgccatggaa ttttgaggaa
14701 gtggtcgaca agggcgcctc tgcccagagc ttcatcgaaa ggatgaccaa ttttgacaaa
14761 aatctgccta acgaaaaggt gcttccgaag cacagcctgt tgtatgaata cttcacagtt
14821 tataacgagc tcactaaggt caagtacgtc acggagggca tgcgtaagcc tgctttcctg
14881 tctggtgaac aaaaaaaggc gattgtggac ctccttttca agacgaaccg taaagttact
14941 gtgaagcaac tgaaagagga ttactttaag aaaattgagt gcttcgacag tgtggagatt
15001 tccggtgtcg aggaccggtt taacgccagc ctgggtacgt atcatgacct gcttaaaatt
15061 atcaaggata aagatttcct ggataatgaa gagaacgaag atatactgga ggacattgtg
15121 ttgactttga ccctcttcga ggacagagag atgattgagg aaagactgaa gacctacgca
15181 cacctttttg atgacaaggt catgaaacaa ctcaagcgcc ggcgctatac tggctggggc
15241 cggctttctc gcaagctcat caatgggatt cgggataagc aatcaggcaa gacaattttg
15301 gacttcctca aatccgacgg attcgcaaat aggaatttta tgcagctgat acatgacgac
15361 tctttgacat tcaaagaaga catacagaag gctcaggtct ccggccaagg agattctttg
15421 cacgagcata tcgctaactt ggcaggtagc cccgccataa aaaagggcat tcttcaaacg
15481 gtaaaagttg ttgacgaact cgtgaaggtt atgggccgtc ataagccgga aaacattgtt
15541 attgaaatgg ctagggaaaa tcagacgacc cagaagggac agaaaaatag cagggagcgg
15601 atgaagagaa ttgaagaggg aattaaggag cttggatctc agattcttaa ggagcaccct
15661 gtggagaaca cccaacttca gaatgaaaag ctctaccttt actaccttca aaacggccgg
15721 gatatgtacg tcgatcagga acttgacatt aaccggttga gcgattatga cgttgacgct
15781 attgtgcccc aatctttcct taaagacgac tctatcgaca ataaagtgct gacgcgcagc
15841 gataaaaatc gcggtaagtc ggataatgtc ccgtcggaag aggtggttaa aaaaatgaag
15901 aactattgga ggcaactcct gaatgccaag ctgatcactc agaggaaatt cgacaatctc
15961 accaaggcag aaaggggtgg acttagcgag ctcgacaagg ccggttttat caaaagacag
16021 ctggtggaga cacgccaaat caccaaacac gttgcccaga tcctggattc gaggatgaac
16081 acgaagtatg acgagaacga caagttgatt agggaagtca aggtcatcac tttgaagtcc
16141 aagctggtga gcgactttcg caaagacttc cagttttaca aagtcaggga aattaataac
16201 taccaccacg cccacgacgc ctaccttaac gccgtggttg gcacagcact catcaagaaa
16261 taccctaagc tcgaatctga gttcgtctat ggcgactata aggtctacga cgttagaaaa
16321 atgatcgcga aatctgagca ggaaataggc aaggcaactg ccaagtactt cttctattcc
16381 aatatcatga acttttttaa gacggagatt accctggcga atggtgagat ccgcaagcgc
16441 cctttgattg agacaaacgg agaaacagga gagatcgtat gggacaaagg gcgggacttt
16501 gctactgtta ggaaggtgct ctctatgcca caagttaaca ttgtcaaaaa aactgaagtg
16561 cagacaggtg ggtttagcaa ggaatctatc ctgccgaaga ggaactctga caagctgatc
16621 gcccgcaaga aagattggga tccgaaaaag tacggaggat tcgactcccc cacagttgcg
16681 tactccgtgc ttgtcgtggc caaagtggag aagggcaagt ctaagaagct caagagcgtc
16741 aaagagttgt tggggatcac gattatggag cggtcgtctt tcgaaaagaa tccgatagat
16801 tttctcgagg ccaagggtta taaagaagtc aagaaggatc ttatcatcaa gctccctaag
16861 tactccctct ttgagcttga aaacggacgg aaaagaatgc tggcttcagc gggtgaactt
16921 cagaagggta atgaactcgc tctgccctca aaatatgtga atttccttta cctggcatca
16981 cactatgaga agcttaaggg gtctccagag gacaacgagc agaagcaact gttcgttgaa
17041 caacacaagc actaccttga cgagattatc gagcaaatca gcgagtttag caagcgcgtt
17101 atactggcag acgcaaatct tgataaggtc cttagcgcct acaacaagca tagagacaaa
17161 cccatccggg agcaggccga gaacattatt catctcttca ccttgacgaa tcttggggcc
17221 ccggccgcgt tcaagtactt cgatactacc atagacagaa agcgctatac atcgacaaag
17281 gaagttcttg acgccacgct gatccaccaa agtataacag gcctctatga gacacgcatc
17341 gacctttcgc agttgggcgg tgacctctct ggcggcagta gtggcggatc ctcaggaagt
17401 gagactcccg gaacatccga atctgctact ccagagagct caggtggcag ctccggaggt
17461 agctccacac ttaacattga agacgaatat cgcctccatg agacaagtaa ggaacccgac
17521 gtttcgcttg gctctacctg gctctccgac ttcccccaag cttgggccga aaccggcggt
17581 atggggcttg ctgttcgcca ggccccattg ataataccgc tgaaagcgac aagcacacca
17641 gtttcgatca aacaataccc aatgtctcag gaggcgaggc tcggcattaa gccgcacatc
17701 caacggttgc tcgaccaggg tattcttgtg ccctgccaat cgccgtggaa cacccccttg
17761 ttgcccgtta agaaaccagg aaccaacgac tacaggccgg tgcaagactt gcgcgaggtg
17821 aacaaacggg tggaggatat tcacccgact gtccccaacc catataattt gctttcaggt
17881 ttgcccccta gccatcagtg gtataccgtg ctggacttga aagatgcctt cttttgtttg
17941 cgcctccacc cgacttcaca gcccctgttt gcgttcgaat ggcgcgatcc ggagatgggt
18001 atttcggggc aacttacgtg gacaagactg ccacagggat ttaaaaattc gccaacactc
18061 ttcaacgagg cccttcacag ggatcttgcc gacttccgca tccagcaccc tgaccttatc
18121 cttctccagt acgtcgacga tttgcttctc gcagctacta gcgaactcga ctgccagcaa
18181 ggcacacggg ctctgctcca gacactgggg aaccttggct accgggcctc tgccaaaaaa
18241 gcgcaaatat gccagaagca ggttaagtac ttggggtatt tgctgaagga ggggcaaagg
18301 tggctcacag aggcacgcaa agaaacggtc atgggccagc ctaccccaaa aacaccaaga
18361 caactgcgcg agtttttggg taaagcagga ttttgccggc tgtttatccc gggtttcgcc
18421 gaaatggccg cacctttgta tccgttgact aagcccggta ctctgtttaa ctggggaccc
18481 gaccaacaaa aagcatatca agagatcaag caggcattgc tgaccgcgcc ggccctgggt
18541 ttgcccgact tgaccaaacc gtttgaattg tttgttgacg aaaagcaggg atacgcaaag
18601 ggcgttctca cgcaaaaact cggaccttgg agaagacccg ttgcatacct tagtaaaaag
18661 ctggatccgg tggccgctgg atggccaccg tgtttgagga tggtcgccgc tattgcggtc
18721 cttacgaagg atgccggcaa actgacgatg gggcaaccac ttgttattct tgcacctcac
18781 gccgtcgagg ctcttgttaa acaaccaccc gatcgctggc tgtctaatgc tcggatgacc
18841 cattaccaag ctcttttgct ggatacagac cgggttcaat tcgggccggt cgtcgccctc
18901 aacccggcca ctttgctgcc gctcccagag gaaggcctgc aacacaattg tcttgacatt
18961 cttgccgagg cccacggtac aaggcccgac ctcaccgacc agcctcttcc ggacgctgac
19021 catacctggt acacagacgg gagcagtctt ctgcaagagg gacagaggaa agcaggggcc
19081 gcagttacca ctgaaactga agtgatatgg gctaaagcat tgcccgcggg aactagcgca
19141 cagcgcgcgg agctcattgc tttgactcag gcactgaaaa tggccgaagg caagaagctg
19201 aatgtctaca ccgactcgag atatgctttc gctactgccc atatacatgg ggagatatat
19261 aggcgcaggg gatggctcac ctctgaaggt aaggagatta agaacaaaga tgagatcctg
19321 gctttgctca aagctttgtt cctccctaag aggttgtcaa taatccattg tcccgggcat
19381 caaaaaggtc attcggcgga ggctaggggt aacaggatgg cggaccaggc agctcgcaag
19441 gccgccatta cagagacacc cgatacctca actttgttga tcgaaaactc cagccccagc
19501 ggaggatcaa aaagaaccgc agacggttcg gaattcgaac ccaaaaagaa gaggaaagtt
19561 tgaactagtg agctcgaatt tccccgatcg ttcaaacatt tggcaataaa gtttcttaag
19621 attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa
19681 gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag
19741 agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga
19801 taaattatcg cgcg
//
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