pSET152

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pSET152链霉菌整合载体基本信息

出品公司: --
载体名称: pset152
质粒类型: 链霉菌整合载体,链霉菌基因过表达载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: 无启动子,需要自己加入相应启动子
载体大小: 5.7 kb
5' 测序引物及序列: M13 (-21) Forward:TGTAAAACGACGGCCAGT  或者M13 (-40) Forword:GTTTTCCCAGTCACGAC
3' 测序引物及序列: M13-R:CAGGAAACAGCTATGAC
载体标签: --
载体抗性: 安普霉素
筛选标记: --
克隆菌株: DH5α 等
宿主细胞(系): 链霉菌,放线菌等
备注: pSET'152载体能够将外源基因整合进入链霉菌基因组中,是链霉菌基因组高表达特定基因时常用的基因导入载体。
产品目录号: --
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

pSET152链霉菌整合载体质粒图谱和多克隆位点信息

pset152载体图谱
pset152载体图谱

pSET152链霉菌整合载体简介

pSET152链霉菌整合载体序列

LOCUS       pSET152                5715 bp    DNA     circular SYN 14-NOV-2006
DEFINITION  Cloning vector PSET152.
ACCESSION   AJ414670
VERSION     AJ414670.1  GI:17974209
KEYWORDS    aac(3)IV gene; apramycin resistance gene; int gene; integrase; lacZ
            alpha peptide; lacZa gene.
SOURCE      Cloning vector pSET152
  ORGANISM  Cloning vector pSET152
            other sequences; artificial sequences; vectors.
REFERENCE   1
  AUTHORS   Wilkinson,C.J., Hughes-Thomas,Z.A., Martin,C.J., Bohm,I.,
            Mironenko,T., Deacon,M., Wheatcroft,M., Wirtz,G., Staunton,J. and
            Leadlay,P.F.
  TITLE     Increasing the efficiency of heterologous promoters in
            actinomycetes
  JOURNAL   J. Mol. Microbiol. Biotechnol. 4 (4), 417-426 (2002)
   PUBMED   12125822
REFERENCE   2  (bases 1 to 5715)
  AUTHORS   Wilkinson,C.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2001) Wilkinson C.J., Department of Biochemistry,
            University of Cambridge, 80 Tennis Court Rd., Cambridge, CB2 1GA,
            UNITED KINGDOM
COMMENT     Related sequences V00635, J02451, M24524, M21778.
FEATURES             Location/Qualifiers
     source          1..5715
                     /organism="Cloning vector pSET152"
                     /mol_type="other DNA"
                     /host="Streptomyces sp."
                     /db_xref="taxon:173260"
                     /lab_host="Escherichia coli"
     gene            complement(163..507)
                     /gene="lacZa"
     CDS             complement(163..507)
                     /gene="lacZa"
                     /function="blue-white screening"
                     /codon_start=1
                     /transl_table=11
                     /product="lacZ alpha peptide"
                     /protein_id="CAC93946.1"
                     /db_xref="GI:17974210"
     variation       complement(508..509)
                     /label="pUC18"
                     /replace="ggt"
     gene            1729..2505
                     /gene="aac(3)IV"
     CDS             1729..2505
                     /gene="aac(3)IV"
                     /function="apramycin resistance"
                     /codon_start=1
                     /transl_table=11
                     /product="apramycin resistance gene"
                     /protein_id="CAC93947.1"
                     /db_xref="GI:17974211"
     rep_origin      3340..3451
                     /label="RP4 origin of single-stranded DNA transfer"
                     /direction=RIGHT
     misc_feature    3799..3837
                     /label="phiC31 attP site"
     gene            3854..5695
                     /gene="int"
     CDS             3854..5695
                     /gene="int"
                     /function="phiC31 integrase"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="CAC93948.1"
                     /db_xref="GI:17974212"
ORIGIN      
        1 atctacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca
       61 gctctcgcag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt
      121 cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttg atcggcactt
      181 tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattta tgcggtgtga
      241 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct
      301 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      361 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      421 ttgtaaaacg acggccagtg ccaagcttgg gctgcaggtc gactctagag gatccgcggc
      481 cgcgcgcgat atcgaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc
      541 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
      601 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
      661 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
      721 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
      781 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
      841 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
      901 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
      961 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     1021 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     1081 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     1141 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     1201 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     1261 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     1321 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     1381 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     1441 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     1501 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     1561 gattttggtc atgagattat caaaaaggat cttcacctag atccttttgg ttcatgtgca
     1621 gctccatcag caaaagggga tgataagttt atcaccaccg actatttgca acagtgccgt
     1681 tgatcgtgct atgatcgact gatgtcatca gcggtggagt gcaatgtcgt gcaatacgaa
     1741 tggcgaaaag ccgagctcat cggtcagctt ctcaaccttg gggttacccc cggcggtgtg
     1801 ctgctggtcc acagctcctt ccgtagcgtc cggcccctcg aagatgggcc acttggactg
     1861 atcgaggccc tgcgtgctgc gctgggtccg ggagggacgc tcgtcatgcc ctcgtggtca
     1921 ggtctggacg acgagccgtt cgatcctgcc acgtcgcccg ttacaccgga ccttggagtt
     1981 gtctctgaca cattctggcg cctgccaaat gtaaagcgca gcgcccatcc atttgccttt
     2041 gcggcagcgg ggccacaggc agagcagatc atctctgatc cattgcccct gccacctcac
     2101 tcgcctgcaa gcccggtcgc ccgtgtccat gaactcgatg ggcaggtact tctcctcggc
     2161 gtgggacacg atgccaacac gacgctgcat cttgccgagt tgatggcaaa ggttccctat
     2221 ggggtgccga gacactgcac cattcttcag gatggcaagt tggtacgcgt cgattatctc
     2281 gagaatgacc actgctgtga gcgctttgcc ttggcggaca ggtggctcaa ggagaagagc
     2341 cttcagaagg aaggtccagt cggtcatgcc tttgctcggt tgatccgctc ccgcgacatt
     2401 gtggcgacag ccctgggtca actgggccga gatccgttga tcttcctgca tccgccagag
     2461 gcgggatgcg aagaatgcga tgccgctcgc cagtcgattg gctgagctca tgagcggaga
     2521 acgagatgac gttggagggg caaggtcgcg ctgattgctg gggcaacacg tggagcggat
     2581 cggggattgt ctttcttcag ctcgctgatg atatgctgac gctcaatgcc gtttggcctc
     2641 cgactaacga aaatcccgca tttggacggc tgatccgatt ggcacggcgg acggcgaatg
     2701 gcggagcaga cgctcgtccg ggggcaatga gatatgaaaa agcctgaact caccgcgacg
     2761 tatcgggccc tggccagcta gctagagtcg acctgcaggt ccccggggat cggtcttgcc
     2821 ttgctcgtcg gtgatgtact tcaccagctc cgcgaagtcg ctcttcttga tggagcgcat
     2881 ggggacgtgc ttggcaatca cgcgcacccc ccggccgttt tagcggctaa aaaagtcatg
     2941 gctctgccct cgggcggacc acgcccatca tgaccttgcc aagctcgtcc tgcttctctt
     3001 cgatcttcgc cagcagggcg aggatcgtgg catcaccgaa ccgcgccgtg cgcgggtcgt
     3061 cggtgagcca gagtttcagc aggccgccca ggcggcccag gtcgccattg atgcgggcca
     3121 gctcgcggac gtgctcatag tccacgacgc ccgtgatttt gtagccctgg ccgacggcca
     3181 gcaggtaggc cgacaggctc atgccggccg ccgccgcctt ttcctcaatc gctcttcgtt
     3241 cgtctggaag gcagtacacc ttgataggtg ggctgccctt cctggttggc ttggtttcat
     3301 cagccatccg cttgccctca tctgttacgc cggcggtagc cggccagcct cgcagagcag
     3361 gattcccgtt gagcaccgcc aggtgcgaat aagggacagt gaagaaggaa cacccgctcg
     3421 cgggtgggcc tacttcacct atcctgcccg gctgacgccg ttggatacac caaggaaagt
     3481 ctacacgaac cctttggcaa aatcctgtat atcgtgcgaa aaaggatgga tataccgaaa
     3541 aaatcgctat aatgaccccg aagcagggtt atgcagcgga aaagatccgt cgacctgcag
     3601 gcatgcaagc tctagcgatt ccagacgtcc cgaaggcgtg gcgcggcttc cccgtgccgg
     3661 agcaatcgcc ctgggtgggt tacacgacgc ccctctatgg cccgtactga cggacacacc
     3721 gaagccccgg cggcaaccct cagcggatgc cccggggctt cacgttttcc caggtcagaa
     3781 gcggttttcg ggagtagtgc cccaactggg gtaacctttg agttctctca gttgggggcg
     3841 tagggtcgcc gacatgacac aaggggttgt gaccggggtg gacacgtacg cgggtgctta
     3901 cgaccgtcag tcgcgcgagc gcgagaattc gagcgcagca agcccagcga cacagcgtag
     3961 cgccaacgaa gacaaggcgg ccgaccttca gcgcgaagtc gagcgcgacg ggggccggtt
     4021 caggttcgtc gggcatttca gcgaagcgcc gggcacgtcg gcgttcggga cggcggagcg
     4081 cccggagttc gaacgcatcc tgaacgaatg ccgcgccggg cggctcaaca tgatcattgt
     4141 ctatgacgtg tcgcgcttct cgcgcctgaa ggtcatggac gcgattccga ttgtctcgga
     4201 attgctcgcc ctgggcgtga cgattgtttc cactcaggaa ggcgtcttcc ggcagggaaa
     4261 cgtcatggac ctgattcacc tgattatgcg gctcgacgcg tcgcacaaag aatcttcgct
     4321 gaagtcggcg aagattctcg acacgaagaa ccttcagcgc gaattgggcg ggtacgtcgg
     4381 cgggaaggcg ccttacggct tcgagcttgt ttcggagacg aaggagatca cgcgcaacgg
     4441 ccgaatggtc aatgtcgtca tcaacaagct tgcgcactcg accactcccc ttaccggacc
     4501 cttcgagttc gagcccgacg taatccggtg gtggtggcgt gagatcaaga cgcacaaaca
     4561 ccttcccttc aagccgggca gtcaagccgc cattcacccg ggcagcatca cggggctttg
     4621 taagcgcatg gacgctgacg ccgtgccgac ccggggcgag acgattggga agaagaccgc
     4681 ttcaagcgcc tgggacccgg caaccgttat gcgaatcctt cgggacccgc gtattgcggg
     4741 cttcgccgct gaggtgatct acaagaagaa gccggacggc acgccgacca cgaagattga
     4801 gggttaccgc attcagcgcg acccgatcac gctccggccg gtcgagcttg attgcggacc
     4861 gatcatcgag cccgctgagt ggtatgagct tcaggcgtgg ttggacggca gggggcgcgg
     4921 caaggggctt tcccgggggc aagccattct gtccgccatg gacaagctgt actgcgagtg
     4981 tggcgccgtc atgacttcga agcgcgggga agaatcgatc aaggactctt accgctgccg
     5041 tcgccggaag gtggtcgacc cgtccgcacc tgggcagcac gaaggcacgt gcaacgtcag
     5101 catggcggca ctcgacaagt tcgttgcgga acgcatcttc aacaagatca ggcacgccga
     5161 aggcgacgaa gagacgttgg cgcttctgtg ggaagccgcc cgacgcttcg gcaagctcac
     5221 tgaggcgcct gagaagagcg gcgaacgggc gaaccttgtt gcggagcgcg ccgacgccct
     5281 gaacgccctt gaagagctgt acgaagaccg cgcggcaggc gcgtacgacg gacccgttgg
     5341 caggaagcac ttccggaagc aacaggcagc gctgacgctc cggcagcaag gggcggaaga
     5401 gcggcttgcc gaacttgaag ccgccgaagc cccgaagctt ccccttgacc aatggttccc
     5461 cgaagacgcc gacgctgacc cgaccggccc taagtcgtgg tgggggcgcg cgtcagtaga
     5521 cgacaagcgc gtgttcgtcg ggctcttcgt agacaagatc gttgtcacga agtcgactac
     5581 gggcaggggg cagggaacgc ccatcgagaa gcgcgcttcg atcacgtggg cgaagccgcc
     5641 gaccgacgac gacgaagacg acgcccagga cggcacggaa gacgtagcgg cgtagcgaga
     5701 cacccgggaa gcctg
//

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