pGL4.72[hRlucCP]

价格:1500元

联系方式:I47-825O-882O

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pGL4.72载体基本信息

出品公司: Promega
载体名称: pGL4.72 ; pGL4.72[hRlucCP]
质粒类型: 信号通路报告载体;哺乳动物载体;海肾荧光素酶报告载体
高拷贝/低拷贝: --
克隆方法: 限制性内切酶,多克隆位点
启动子:
载体大小: 3704 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: hRlucCP
载体抗性: 氨苄青霉素
筛选标记:
克隆菌株: TOP10等常规菌株
宿主细胞(系): HEK293等
备注: pGL4.72[hRlucCP]载体是信号通路报告载体;无启动子;含海肾 荧光素酶报告基因hRluc。
产品目录号: E6901
稳定性: 瞬表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

pGL4.72载体质粒图谱和多克隆位点信息

pGL4.72载体图谱



pGL4.72 载体特征

pGL4.72载体简介

pGL4.72载体简介
The pGL4.72[hRlucCP] Vector encodes the luciferase reporter gene hRlucCP (Renilla reniformis) and is designed for high expression and reduced anomalous transcription. The pGL4 Vectors are engineered with fewer consensus regulatory sequences and a synthetic gene, which has been codon optimized for mammalian expression. 
The pGL4.72[hRlucCP] Vector has no promoter. However, it contains a multiple cloning region that allows for cloning of a promoter of choice. The hRlucCP reporter gene contains two protein destabilization sequences, hCL1 and hPEST. The protein encoded by hRlucCP responds more quickly and with greater magnitude to changes in transcriptional activity than the hRluc gene, its more stable counterpart.

pGL4.72载体序列

LOCUS       AY738228                3704 bp    DNA     circular SYN 08-NOV-2004
DEFINITION  Renilla luciferase reporter vector pGL4.72[hRlucCP], complete
            sequence.
ACCESSION   AY738228
VERSION     AY738228.1  GI:55535636
KEYWORDS    .
SOURCE      Renilla luciferase reporter vector pGL4.72[hRlucCP]
  ORGANISM  Renilla luciferase reporter vector pGL4.72[hRlucCP]
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 3704)
  AUTHORS   Almond,B., Chauvin,F. and Browning,J.
  TITLE     pGL4 Luciferase Reporter Vectors Technical Manual, TM259, Promega
            Corporation
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3704)
  AUTHORS   Almond,B., Chauvin,F. and Browning,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2004) Scientific Communications, Promega
            Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA
FEATURES             Location/Qualifiers
     source          1..3704
                     /organism="Renilla luciferase reporter vector
                     pGL4.72[hRlucCP]"
                     /mol_type="other DNA"
                     /db_xref="taxon:296665"
     misc_feature    1..70
                     /note="multiple cloning site region"
     CDS             100..1212
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase hRlucCP"
                     /protein_id="AAV52881.1"
                     /db_xref="GI:55535637"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAE
                     NAVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLT
                     AWFELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIE
                     EDIALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLS
                     WPREIPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNT
                     EFVKVKGLHFSQEDAPDEMGKYIKSFVERVLKNEQNSACKNWFSSLSHFVIHLNSHGF
                     PPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     misc_feature    1249..1470
                     /note="SV40 late poly(A) region"
     primer_bind     1538..1557
                     /note="reporter vector primer 4 (RVPrimer4)"
     rep_origin      1795
                     /note="ColE1-derived"
     gene            complement(2586..3446)
                     /gene="ampR"
     CDS             complement(2586..3446)
                     /gene="ampR"
                     /note="AmpR"
                     /codon_start=1
                     /transl_table=11
                     /product="synthetic beta-lactamase"
                     /protein_id="AAV52882.1"
                     /db_xref="GI:55535638"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     regulatory      3551..3704
                     /regulatory_class="polyA_signal_sequence"
                     /note="synthetic; transcriptional pause site"
     primer_bind     complement(3653..3672)
                     /note="reporter vector primer 3 (RVPrimer3)"
ORIGIN      
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
       61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tggcttccaa ggtgtacgac
      121 cccgagcaac gcaaacgcat gatcactggg cctcagtggt gggctcgctg caagcaaatg
      181 aacgtgctgg actccttcat caactactat gattccgaga agcacgccga gaacgccgtg
      241 atttttctgc atggtaacgc tgcctccagc tacctgtgga ggcacgtcgt gcctcacatc
      301 gagcccgtgg ctagatgcat catccctgat ctgatcggaa tgggtaagtc cggcaagagc
      361 gggaatggct catatcgcct cctggatcac tacaagtacc tcaccgcttg gttcgagctg
      421 ctgaaccttc caaagaaaat catctttgtg ggccacgact ggggggcttg tctggccttt
      481 cactactcct acgagcacca agacaagatc aaggccatcg tccatgctga gagtgtcgtg
      541 gacgtgatcg agtcctggga cgagtggcct gacatcgagg aggatatcgc cctgatcaag
      601 agcgaagagg gcgagaaaat ggtgcttgag aataacttct tcgtcgagac catgctccca
      661 agcaagatca tgcggaaact ggagcctgag gagttcgctg cctacctgga gccattcaag
      721 gagaagggcg aggttagacg gcctaccctc tcctggcctc gcgagatccc tctcgttaag
      781 ggaggcaagc ccgacgtcgt ccagattgtc cgcaactaca acgcctacct tcgggccagc
      841 gacgatctgc ctaagatgtt catcgagtcc gaccctgggt tcttttccaa cgctattgtc
      901 gagggagcta agaagttccc taacaccgag ttcgtgaagg tgaagggcct ccacttcagc
      961 caggaggacg ctccagatga aatgggtaag tacatcaaga gcttcgtgga gcgcgtgctg
     1021 aagaacgagc agaattctgc ttgcaagaac tggttcagta gcttaagcca ctttgtgatc
     1081 caccttaaca gccacggctt ccctcccgag gtggaggagc aggccgccgg caccctgccc
     1141 atgagctgcg cccaggagag cggcatggat agacaccctg ctgcttgcgc cagcgccagg
     1201 atcaacgtct aactgcagtc tagagtcggg gcggccggcc gcttcgagca gacatgataa
     1261 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     1321 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
     1381 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
     1441 aaagcaagta aaacctctac aaatgtggta aaatcgataa ggatccgtcg accgatgccc
     1501 ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc
     1561 gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctcttc
     1621 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     1681 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     1741 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     1801 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     1861 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     1921 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     1981 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     2041 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     2101 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     2161 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     2221 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     2281 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     2341 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     2401 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     2461 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     2521 aatctaaagt atatatgagt aaacttggtc tgacagcggc cgcaaatgct aaaccactgc
     2581 agtggttacc agtgcttgat cagtgaggca ccgatctcag cgatctgcct atttcgttcg
     2641 tccatagtgg cctgactccc cgtcgtgtag atcactacga ttcgtgaggg cttaccatca
     2701 ggccccagcg cagcaatgat gccgcgagag ccgcgttcac cggcccccga tttgtcagca
     2761 atgaaccagc cagcagggag ggccgagcga agaagtggtc ctgctacttt gtccgcctcc
     2821 atccagtcta tgagctgctg tcgtgatgct agagtaagaa gttcgccagt gagtagtttc
     2881 cgaagagttg tggccattgc tactggcatc gtggtatcac gctcgtcgtt cggtatggct
     2941 tcgttcaact ctggttccca gcggtcaagc cgggtcacat gatcacccat attatgaaga
     3001 aatgcagtca gctccttagg gcctccgatc gttgtcagaa gtaagttggc cgcggtgttg
     3061 tcgctcatgg taatggcagc actacacaat tctcttaccg tcatgccatc cgtaagatgc
     3121 ttttccgtga ccggcgagta ctcaaccaag tcgttttgtg agtagtgtat acggcgacca
     3181 agctgctctt gcccggcgtc tatacgggac aacaccgcgc cacatagcag tactttgaaa
     3241 gtgctcatca tcgggaatcg ttcttcgggg cggaaagact caaggatctt gccgctattg
     3301 agatccagtt cgatatagcc cactcttgca cccagttgat cttcagcatc ttttactttc
     3361 accagcgttt cggggtgtgc aaaaacaggc aagcaaaatg ccgcaaagaa gggaatgagt
     3421 gcgacacgaa aatgttggat gctcatactc gtcctttttc aatattattg aagcatttat
     3481 cagggttact agtacgtctc tcaaggataa gtaagtaata ttaaggtacg ggaggtattg
     3541 gacaggccgc aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtga
     3601 atcgatagta ctaacatacg ctctccatca aaacaaaacg aaacaaaaca aactagcaaa
     3661 ataggctgtc cccagtgcaa gtgcaggtgc cagaacattt ctct
//

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