pBBR1MCS-2

价格:1500元

联系方式:I47-825O-882O

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pBBR1MCS2载体基本信息

出品公司: --
载体名称: pBBR1MCS-2, pBBR1MCS2, pBBRMCS2
质粒类型: 广宿主蛋白表达或克隆载体质粒,广宿主载体质粒
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: --
载体大小: 5.1 kb
5' 测序引物及序列: M13-F: TGTAAAACGACGGCCAGT
3' 测序引物及序列: M13-R: CAGGAAACAGCTATGAC
载体标签: --
载体抗性: Kanamycin.html' target='_blank'>卡那霉素
筛选标记: --
克隆菌株: DH5α 等
宿主细胞(系): 多种微生物物种,是广宿主质粒。
备注: 参考文献:
[1] Kovach ME, Phillips RW, Elzer PH, Roop RM, 2nd, Peterson KM. pBBR1MCS: a broad-host-range cloning vector. BioTechniques 1994;16:800-2.

[2] Kovach ME, Elzer PH, Hill DS, Robertson GT, Farris MA, Roop RM, 2nd, et al. Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes. Gene 1995;166:175-6.

GenBank sequence:U23751
产品目录号: --
稳定性/瞬表达: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

pBBR1MCS2载体质粒图谱和多克隆位点信息

pBBR1MCS-2载体图谱
pBBR1MCS-2载体图谱

pBBR1MCS2载体简介

pBBR1MCS2载体序列

LOCUS       pBBR1MCS-2                   5144 bp    DNA     circular SYN 10-JAN-2005
DEFINITION  Cloning vector pBBR1MCS-2 plasmid pBBR1MCS-2, complete sequence.
ACCESSION   U23751
VERSION     U23751.1  GI:773412
KEYWORDS    .
SOURCE      Cloning vector pBBR1MCS-2
  ORGANISM  Cloning vector pBBR1MCS-2
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 5144)
  AUTHORS   Kovach,M.E., Phillips,R.W., Elzer,P.H., Roop,R.M. II and
            Peterson,K.M.
  TITLE     pBBR1MCS: a broad-host-range cloning vector
  JOURNAL   BioTechniques 16 (5), 800-802 (1994)
   PUBMED   8068328
REFERENCE   2  (bases 1 to 5144)
  AUTHORS   Kovach,M.E., Elzer,P.H., Hill,D.S., Robertson,G.T., Farris,M.A.,
            Roop,R.M. II and Peterson,K.M.
  TITLE     Four new derivatives of the broad-host-range cloning vector
            pBBR1MCS, carrying different antibiotic-resistance cassettes
  JOURNAL   Gene 166 (1), 175-176 (1995)
   PUBMED   8529885
REFERENCE   3  (bases 1 to 5144)
  AUTHORS   Kovach,M.E., Elzer,P.H., Hill,S., Robertson,G.T., Ferris,M.,
            Roop,R.M. and Peterson,K.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-1995) Michael E. Kovach, Microbiology, Louisiana
            State University Medical Center, 1501 Kings Highway, Shreveport, LA
            71130, USA
FEATURES             Location/Qualifiers
     source          1..5144
                     /organism="Cloning vector pBBR1MCS-2"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:39477"
                     /lab_host="Bordetella bronchiseptica"
                     /plasmid="pBBR1MCS-2"
                     /note="modification of cloning vector pBBR1MCS, GenBank
                     Accession Number U02374; gene encoding chloramphenical
                     resistance replaced by gene encoding kanamycin resistance"
     gene            complement(join(4945..5144,1..799))
                     /gene="mob"
     CDS             complement(join(4945..5144,1..799))
                     /gene="mob"
                     /function="required for plasmid mobilization"
                     /codon_start=1
                     /transl_table=11
                     /product="Mob"
                     /protein_id="AAB05991.1"
                     /db_xref="GI:773416"
     gene            1793..2455
                     /gene="rep"
     CDS             1793..2455
                     /gene="rep"
                     /function="required for plasmid replication"
                     /codon_start=1
                     /transl_table=11
                     /product="Rep"
                     /protein_id="AAB05988.1"
                     /db_xref="GI:773413"
     gene            complement(3013..3378)
                     /gene="lacZ"
     CDS             complement(3013..3378)
                     /gene="lacZ"
                     /note="alpha peptide of LacZ"
                     /codon_start=1
                     /transl_table=11
                     /product="beta galactosidase"
                     /protein_id="AAB05989.1"
                     /db_xref="GI:773414"
     CDS             complement(3893..4687)
                     /codon_start=1
                     /transl_table=11
                     /product="kanamycin resistance determinant"
                     /protein_id="AAB05990.1"
                     /db_xref="GI:773415"
ORIGIN      
        1 ctcgggccgt ctcttgggct tgatcggcct tcttgcgcat ctcacgcgct cctgcggcgg
       61 cctgtagggc aggctcatac ccctgccgaa ccgcttttgt cagccggtcg gccacggctt
      121 ccggcgtctc aacgcgcttt gagattccca gcttttcggc caatccctgc ggtgcatagg
      181 cgcgtggctc gaccgcttgc gggctgatgg tgacgtggcc cactggtggc cgctccaggg
      241 cctcgtagaa cgcctgaatg cgcgtgtgac gtgccttgct gccctcgatg ccccgttgca
      301 gccctagatc ggccacagcg gccgcaaacg tggtctggtc gcgggtcatc tgcgctttgt
      361 tgccgatgaa ctccttggcc gacagcctgc cgtcctgcgt cagcggcacc acgaacgcgg
      421 tcatgtgcgg gctggtttcg tcacggtgga tgctggccgt cacgatgcga tccgccccgt
      481 acttgtccgc cagccacttg tgcgccttct cgaagaacgc cgcctgctgt tcttggctgg
      541 ccgacttcca ccattccggg ctggccgtca tgacgtactc gaccgccaac acagcgtcct
      601 tgcgccgctt ctctggcagc aactcgcgca gtcggcccat cgcttcatcg gtgctgctgg
      661 ccgcccagtg ctcgttctct ggcgtcctgc tggcgtcagc gttgggcgtc tcgcgctcgc
      721 ggtaggcgtg cttgagactg gccgccacgt tgcccatttt cgccagcttc ttgcatcgca
      781 tgatcgcgta tgccgccatg cctgcccctc ccttttggtg tccaaccggc tcgacggggg
      841 cagcgcaagg cggtgcctcc ggcgggccac tcaatgcttg agtatactca ctagactttg
      901 cttcgcaaag tcgtgaccgc ctacggcggc tgcggcgccc tacgggcttg ctctccgggc
      961 ttcgccctgc gcggtcgctg cgctcccttg ccagcccgtg gatatgtgga cgatggccgc
     1021 gagcggccac cggctggctc gcttcgctcg gcccgtggac aaccctgctg gacaagctga
     1081 tggacaggct gcgcctgccc acgagcttga ccacagggat tgcccaccgg ctacccagcc
     1141 ttcgaccaca tacccaccgg ctccaactgc gcggcctgcg gccttgcccc atcaattttt
     1201 ttaattttct ctggggaaaa gcctccggcc tgcggcctgc gcgcttcgct tgccggttgg
     1261 acaccaagtg gaaggcgggt caaggctcgc gcagcgaccg cgcagcggct tggccttgac
     1321 gcgcctggaa cgacccaagc ctatgcgagt gggggcagtc gaaggcgaag cccgcccgcc
     1381 tgccccccga gcctcacggc ggcgagtgcg ggggttccaa gggggcagcg ccaccttggg
     1441 caaggccgaa ggccgcgcag tcgatcaaca agccccggag gggccacttt ttgccggagg
     1501 gggagccgcg ccgaaggcgt gggggaaccc cgcaggggtg cccttctttg ggcaccaaag
     1561 aactagatat agggcgaaat gcgaaagact taaaaatcaa caacttaaaa aaggggggta
     1621 cgcaacagct cattgcggca ccccccgcaa tagctcattg cgtaggttaa agaaaatctg
     1681 taattgactg ccacttttac gcaacgcata attgttgtcg cgctgccgaa aagttgcagc
     1741 tgattgcgca tggtgccgca accgtgcggc accctaccgc atggagataa gcatggccac
     1801 gcagtccaga gaaatcggca ttcaagccaa gaacaagccc ggtcactggg tgcaaacgga
     1861 acgcaaagcg catgaggcgt gggccgggct tattgcgagg aaacccacgg cggcaatgct
     1921 gctgcatcac ctcgtggcgc agatgggcca ccagaacgcc gtggtggtca gccagaagac
     1981 actttccaag ctcatcggac gttctttgcg gacggtccaa tacgcagtca aggacttggt
     2041 ggccgagcgc tggatctccg tcgtgaagct caacggcccc ggcaccgtgt cggcctacgt
     2101 ggtcaatgac cgcgtggcgt ggggccagcc ccgcgaccag ttgcgcctgt cggtgttcag
     2161 tgccgccgtg gtggttgatc acgacgacca ggacgaatcg ctgttggggc atggcgacct
     2221 gcgccgcatc ccgaccctgt atccgggcga gcagcaacta ccgaccggcc ccggcgagga
     2281 gccgcccagc cagcccggca ttccgggcat ggaaccagac ctgccagcct tgaccgaaac
     2341 ggaggaatgg gaacggcgcg ggcagcagcg cctgccgatg cccgatgagc cgtgttttct
     2401 ggacgatggc gagccgttgg agccgccgac acgggtcacg ctgccgcgcc ggtagcactt
     2461 gggttgcgca gcaacccgta agtgcgctgt tccagactat cggctgtagc cgcctcgccg
     2521 ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga
     2581 cctgaatgga agccggcggc acctcgctaa cggattcacc gtttttatca ggctctggga
     2641 ggcagaataa atgatcatat cgtcaattat tacctccacg gggagagcct gagcaaactg
     2701 gcctcaggca tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtaaa
     2761 ccagcaatag acataagcgg ctatttaacg accctgccct gaaccgacga ccgggtcgaa
     2821 tttgctttcg aatttctgcc attcatccgc ttattatcac ttattcaggc gtagcaccag
     2881 gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc
     2941 agtcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     3001 aaatattaac gcttacaatt tccattcgcc attcaggctg cgcaactgtt gggaagggcg
     3061 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     3121 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
     3181 gcgcgcgtaa tacgactcac tatagggcga attggagctc caccgcggtg gcggccgctc
     3241 tagaactagt ggatcccccg ggctgcagga attcgatatc aagcttatcg ataccgtcga
     3301 cctcgagggg gggcccggta cccagctttt gttcccttta gtgagggtta attgcgcgct
     3361 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     3421 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     3481 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     3541 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgcatgcata
     3601 aaaactgttg taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat
     3661 gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg
     3721 gggtgggcga agaactccag catgagatcc ccgcgctgga ggatcatcca gccggcgtcc
     3781 cggaaaacga ttccgaagcc caacctttca tagaaggcgg cggtggaatc gaaatctcgt
     3841 gatggcaggt tgggcgtcgc ttggtcggtc atttcgaacc ccagagtccc gctcagaaga
     3901 actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa
     3961 gcacgaggaa gcggtcagcc cattcgccgc caagctcttc agcaatatca cgggtagcca
     4021 acgctatgtc ctgatagcgg tccgccacac ccagccggcc acagtcgatg aatccagaaa
     4081 agcggccatt ttccaccatg atattcggca agcaggcatc gccatgggtc acgacgagat
     4141 cctcgccgtc gggcatgcgc gccttgagcc tggcgaacag ttcggctggc gcgagcccct
     4201 gatgctcttc gtccagatca tcctgatcga caagaccggc ttccatccga gtacgtgctc
     4261 gctcgatgcg atgtttcgct tggtggtcga atgggcaggt agccggatca agcgtatgca
     4321 gccgccgcat tgcatcagcc atgatggata ctttctcggc aggagcaagg tgagatgaca
     4381 ggagatcctg ccccggcact tcgcccaata gcagccagtc ccttcccgct tcagtgacaa
     4441 cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct
     4501 cgtcctgcag ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc
     4561 cctgcgctga cagccggaac acggcggcat cagagcagcc gattgtctgt tgtgcccagt
     4621 catagccgaa tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt
     4681 caatcatgcg aaacgatcct catcctgtct cttgatcaga tcttgatccc ctgcgccatc
     4741 agatccttgg cggcaagaaa gccatccagt ttactttgca gggcttccca accttaccag
     4801 agggcgcccc agctggcaat tccggttcgc ttgctgtcca taaaaccgcc cagtctagct
     4861 atcgccatgt aagcccactg caagctacct gctttctctt tgcgcttgcg ttttcccttg
     4921 tccagatagc ccagtagctg acattcatcc caggtggcac ttttcgggga aatgtgcgcg
     4981 cccgcgttcc tgctggcgct gggcctgttt ctggcgctgg acttcccgct gttccgtcag
     5041 cagcttttcg cccacggcct tgatgatcgc ggcggccttg gcctgcatat cccgattcaa
     5101 cggccccagg gcgtccagaa cgggcttcag gcgctcccga aggt
//

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pBBR1MCS2载体应用举例

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