| 质粒名称: | pCW-Cas9 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 50661 |
| 存储实验室: | Eric Lander, David Sabatini |
|
载体骨架基本信息 |
|
| 载体名称: | pCW57.1 |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 7600 bp |
| 完整质粒大小: | 11900 bp |
| 载体类型: | 哺乳细胞表达,慢病毒载体,CRISPR |
| 筛选标记: | Puromycin |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | humanized S. pyogenes Cas9 |
| 插入大小: | 4300 bp |
| 启动子 | Tet ON |
| 标签/融合蛋白: | 3xFLAG(N端) |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | NheI(未破坏) |
| 3' 克隆位点: | BamHI (未破坏) |
| 5' 测序引物 | AGCTCGTTTAGTGAACCGTCAGATC |
| 3' 测序引物 | CACATTCTTCACGTCCGTTC |
|
文献引用方法 |
|
| 材料和方法部分: | pCW-Cas9 was a gift from Eric Lander & David Sabatini (Addgene plasmid # 50661) |
| 参考文献部分: | Genetic screens in human cells using the CRISPR-Cas9 system. Wang T, Wei JJ, Sabatini DM, Lander ES. Science. 2014 Jan 3;343(6166):80-4. doi: 10.1126/science.1246981. Epub 2013 Dec 12. 10.1126/science.1246981 PubMed 24336569 |
买家导航

pCW-Cas9载体质粒基本信息
pCW-Cas9质粒图谱载体图谱和pCW-Cas9载体序列质粒序列多克隆位点信息

pCW-Cas9质粒载体简介
pCW-Cas9质粒序列载体序列
LOCUS pCW-Cas9 Sequencing Result 11885 bp DNA SYN
DEFINITION pCW-Cas9 Sequencing Result
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..11885
/organism="pCW-Cas9 Sequencing Result"
/mol_type="other DNA"
misc_feature 45..60
/label="cPPT"
CDS complement(369..5348)
/label="ORF frame 1"
misc_feature 497..516
/label="pCEP_fwd_primer"
misc_feature 499..523
/label="LNCX_primer"
CDS 549..4820
/label="ORF frame 3"
misc_feature 552..572
/label="FLAG"
misc_feature 573..593
/label="FLAG"
misc_feature 594..617
/label="FLAG"
misc_feature 624..644
/label="SV40 NLS"
misc_feature 624..647
/label="SV40 NLS"
misc_feature 630..644
/label="NLS"
CDS 5119..6744
/label="ORF frame 1"
gene 5347..5934
/label="puro (variant)"
/gene="puro (variant)"
misc_feature 6781..7308
/label="WPRE"
CDS 6821..7345
/label="ORF frame 2"
misc_feature 7401..7416
/label="cPPT"
misc_feature 7401..7422
/label="U3PPT"
misc_feature 7418..7470
/label="delta_U3"
misc_feature 7471..7651
/label="truncHIV-1_3_LTR"
misc_feature 7471..7651
/label="HIV-1_5_LTR"
terminator 7726..7845
/label="SV40_PA_terminator"
misc_feature 7814..7833
/label="EBV_rev_primer"
rep_origin 7863..7961
/label="SV40_origin"
misc_feature 7946..7965
/label="SV40pro_F_primer"
promoter 8021..8049
/label="AmpR_promoter"
gene 8091..8951
/label="Ampicillin"
/gene="Ampicillin"
CDS 8091..8951
/label="ORF frame 3"
rep_origin 9106..9725
/label="pBR322_origin"
promoter 10034..10063
/label="lac_promoter"
misc_feature 10077..10099
/label="M13_pUC_rev_primer"
promoter 10098..10116
/label="M13_reverse_primer"
promoter 10133..10152
/label="T3_promoter"
misc_feature 10406..10586
/label="truncHIV-1_3_LTR"
misc_feature 10406..10586
/label="HIV-1_5_LTR"
misc_feature 10697..10741
/label="HIV-1_psi_pack"
CDS 11129..410
/label="ORF frame 2"
misc_feature 11251..11484
/label="RRE"
ORIGIN
1 AATTCTCGAC CTCGAGACAA ATGGCAGTAT TCATCCACAA TTTTAAAAGA AAAGGGGGGA
61 TTGGGGGGTA CAGTGCAGGG GAAAGAATAG TAGACATAAT AGCAACAGAC ATACAAACTA
121 AAGAATTACA AAAACAAATT ACAAAAATTC AAAATTTTCG GGTTTATTAC AGGGACAGCA
181 GAGATCCACT TTGGCCGCGA ATCGATATGT CGAGTTTACT CCCTATCAGT GATAGAGAAC
241 GTATGTCGAG TTTACTCCCT ATCAGTGATA GAGAACGATG TCGAGTTTAC TCCCTATCAG
301 TGATAGAGAA CGTATGTCGA GTTTACTCCC TATCAGTGAT AGAGAACGTA TGTCGAGTTT
361 ACTCCCTATC AGTGATAGAG AACGTATGTC GAGTTTATCC CTATCAGTGA TAGAGAACGT
421 ATGTCGAGTT TACTCCCTAT CAGTGATAGA GAACGTATGT CGAGGTAGGC GTGTACGGTG
481 GGAGGCCTAT ATAAGCAGAG CTCGTTTAGT GAACCGTCAG ATCGCCTGGA GAATTGGCTA
541 GCgccaccAT GGACTATAAG GACCACGACG GAGACTACAA GGATCATGAT ATTGATTACA
601 AAGACGATGA CGATAAGATG GCCCCAAAGA AGAAGCGGAA GGTCGGTATC CACGGAGTCC
661 CAGCAGCCGA CAAGAAGTAC AGCATCGGCC TGGACATCGG CACCAACTCT GTGGGCTGGG
721 CCGTGATCAC CGACGAGTAC AAGGTGCCCA GCAAGAAATT CAAGGTGCTG GGCAACACCG
781 ACCGGCACAG CATCAAGAAG AACCTGATCG GAGCCCTGCT GTTCGACAGC GGCGAAACAG
841 CCGAGGCCAC CCGGCTGAAG AGAACCGCCA GAAGAAGATA CACCAGACGG AAGAACCGGA
901 TCTGCTATCT GCAAGAGATC TTCAGCAACG AGATGGCCAA GGTGGACGAC AGCTTCTTCC
961 ACAGACTGGA AGAGTCCTTC CTGGTGGAAG AGGATAAGAA GCACGAGCGG CACCCCATCT
1021 TCGGCAACAT CGTGGACGAG GTGGCCTACC ACGAGAAGTA CCCCACCATC TACCACCTGA
1081 GAAAGAAACT GGTGGACAGC ACCGACAAGG CCGACCTGCG GCTGATCTAT CTGGCCCTGG
1141 CCCACATGAT CAAGTTCCGG GGCCACTTCC TGATCGAGGG CGACCTGAAC CCCGACAACA
1201 GCGACGTGGA CAAGCTGTTC ATCCAGCTGG TGCAGACCTA CAACCAGCTG TTCGAGGAAA
1261 ACCCCATCAA CGCCAGCGGC GTGGACGCCA AGGCCATCCT GTCTGCCAGA CTGAGCAAGA
1321 GCAGACGGCT GGAAAATCTG ATCGCCCAGC TGCCCGGCGA GAAGAAGAAT GGCCTGTTCG
1381 GAAACCTGAT TGCCCTGAGC CTGGGCCTGA CCCCCAACTT CAAGAGCAAC TTCGACCTGG
1441 CCGAGGATGC CAAACTGCAG CTGAGCAAGG ACACCTACGA CGACGACCTG GACAACCTGC
1501 TGGCCCAGAT CGGCGACCAG TACGCCGACC TGTTTCTGGC CGCCAAGAAC CTGTCCGACG
1561 CCATCCTGCT GAGCGACATC CTGAGAGTGA ACACCGAGAT CACCAAGGCC CCCCTGAGCG
1621 CCTCTATGAT CAAGAGATAC GACGAGCACC ACCAGGACCT GACCCTGCTG AAAGCTCTCG
1681 TGCGGCAGCA GCTGCCTGAG AAGTACAAAG AGATTTTCTT CGACCAGAGC AAGAACGGCT
1741 ACGCCGGCTA CATTGACGGC GGAGCCAGCC AGGAAGAGTT CTACAAGTTC ATCAAGCCCA
1801 TCCTGGAAAA GATGGACGGC ACCGAGGAAC TGCTCGTGAA GCTGAACAGA GAGGACCTGC
1861 TGCGGAAGCA GCGGACCTTC GACAACGGCA GCATCCCCCA CCAGATCCAC CTGGGAGAGC
1921 TGCACGCCAT TCTGCGGCGG CAGGAAGATT TTTACCCATT CCTGAAGGAC AACCGGGAAA
1981 AGATCGAGAA GATCCTGACC TTCCGCATCC CCTACTACGT GGGCCCTCTG GCCAGGGGAA
2041 ACAGCAGATT CGCCTGGATG ACCAGAAAGA GCGAGGAAAC CATCACCCCC TGGAACTTCG
2101 AGGAAGTGGT GGACAAGGGC GCTTCCGCCC AGAGCTTCAT CGAGCGGATG ACCAACTTCG
2161 ATAAGAACCT GCCCAACGAG AAGGTGCTGC CCAAGCACAG CCTGCTGTAC GAGTACTTCA
2221 CCGTGTATAA CGAGCTGACC AAAGTGAAAT ACGTGACCGA GGGAATGAGA AAGCCCGCCT
2281 TCCTGAGCGG CGAGCAGAAA AAGGCCATCG TGGACCTGCT GTTCAAGACC AACCGGAAAG
2341 TGACCGTGAA GCAGCTGAAA GAGGACTACT TCAAGAAAAT CGAGTGCTTC GACTCCGTGG
2401 AAATCTCCGG CGTGGAAGAT CGGTTCAACG CCTCCCTGGG CACATACCAC GATCTGCTGA
2461 AAATTATCAA GGACAAGGAC TTCCTGGACA ATGAGGAAAA CGAGGACATT CTGGAAGATA
2521 TCGTGCTGAC CCTGACACTG TTTGAGGACA GAGAGATGAT CGAGGAACGG CTGAAAACCT
2581 ATGCCCACCT GTTCGACGAC AAAGTGATGA AGCAGCTGAA GCGGCGGAGA TACACCGGCT
2641 GGGGCAGGCT GAGCCGGAAG CTGATCAACG GCATCCGGGA CAAGCAGTCC GGCAAGACAA
2701 TCCTGGATTT CCTGAAGTCC GACGGCTTCG CCAACAGAAA CTTCATGCAG CTGATCCACG
2761 ACGACAGCCT GACCTTTAAA GAGGACATCC AGAAAGCCCA GGTGTCCGGC CAGGGCGATA
2821 GCCTGCACGA GCACATTGCC AATCTGGCCG GCAGCCCCGC CATTAAGAAG GGCATCCTGC
2881 AGACAGTGAA GGTGGTGGAC GAGCTCGTGA AAGTGATGGG CCGGCACAAG CCCGAGAACA
2941 TCGTGATCGA AATGGCCAGA GAGAACCAGA CCACCCAGAA GGGACAGAAG AACAGCCGCG
3001 AGAGAATGAA GCGGATCGAA GAGGGCATCA AAGAGCTGGG CAGCCAGATC CTGAAAGAAC
3061 ACCCCGTGGA AAACACCCAG CTGCAGAACG AGAAGCTGTA CCTGTACTAC CTGCAGAATG
3121 GGCGGGATAT GTACGTGGAC CAGGAACTGG ACATCAACCG GCTGTCCGAC TACGATGTGG
3181 ACCATATCGT GCCTCAGAGC TTTCTGAAGG ACGACTCCAT CGACAACAAG GTGCTGACCA
3241 GAAGCGACAA GAACCGGGGC AAGAGCGACA ACGTGCCCTC CGAAGAGGTC GTGAAGAAGA
3301 TGAAGAACTA CTGGCGGCAG CTGCTGAACG CCAAGCTGAT TACCCAGAGA AAGTTCGACA
3361 ATCTGACCAA GGCCGAGAGA GGCGGCCTGA GCGAACTGGA TAAGGCCGGC TTCATCAAGA
3421 GACAGCTGGT GGAAACCCGG CAGATCACAA AGCACGTGGC ACAGATCCTG GACTCCCGGA
3481 TGAACACTAA GTACGACGAG AATGACAAGC TGATCCGGGA AGTGAAAGTG ATCACCCTGA
3541 AGTCCAAGCT GGTGTCCGAT TTCCGGAAGG ATTTCCAGTT TTACAAAGTG CGCGAGATCA
3601 ACAACTACCA CCACGCCCAC GACGCCTACC TGAACGCCGT CGTGGGAACC GCCCTGATCA
3661 AAAAGTACCC TAAGCTGGAA AGCGAGTTCG TGTACGGCGA CTACAAGGTG TACGACGTGC
3721 GGAAGATGAT CGCCAAGAGC GAGCAGGAAA TCGGCAAGGC TACCGCCAAG TACTTCTTCT
3781 ACAGCAACAT CATGAACTTT TTCAAGACCG AGATTACCCT GGCCAACGGC GAGATCCGGA
3841 AGCGGCCTCT GATCGAGACA AACGGCGAAA CCGGGGAGAT CGTGTGGGAT AAGGGCCGGG
3901 ATTTTGCCAC CGTGCGGAAA GTGCTGAGCA TGCCCCAAGT GAATATCGTG AAAAAGACCG
3961 AGGTGCAGAC AGGCGGCTTC AGCAAAGAGT CTATCCTGCC CAAGAGGAAC AGCGATAAGC
4021 TGATCGCCAG AAAGAAGGAC TGGGACCCTA AGAAGTACGG CGGCTTCGAC AGCCCCACCG
4081 TGGCCTATTC TGTGCTGGTG GTGGCCAAAG TGGAAAAGGG CAAGTCCAAG AAACTGAAGA
4141 GTGTGAAAGA GCTGCTGGGG ATCACCATCA TGGAAAGAAG CAGCTTCGAG AAGAATCCCA
4201 TCGACTTTCT GGAAGCCAAG GGCTACAAAG AAGTGAAAAA GGACCTGATC ATCAAGCTGC
4261 CTAAGTACTC CCTGTTCGAG CTGGAAAACG GCCGGAAGAG AATGCTGGCC TCTGCCGGCG
4321 AACTGCAGAA GGGAAACGAA CTGGCCCTGC CCTCCAAATA TGTGAACTTC CTGTACCTGG
4381 CCAGCCACTA TGAGAAGCTG AAGGGCTCCC CCGAGGATAA TGAGCAGAAA CAGCTGTTTG
4441 TGGAACAGCA CAAGCACTAC CTGGACGAGA TCATCGAGCA GATCAGCGAG TTCTCCAAGA
4501 GAGTGATCCT GGCCGACGCT AATCTGGACA AAGTGCTGTC CGCCTACAAC AAGCACCGGG
4561 ATAAGCCCAT CAGAGAGCAG GCCGAGAATA TCATCCACCT GTTTACCCTG ACCAATCTGG
4621 GAGCCCCTGC CGCCTTCAAG TACTTTGACA CCACCATCGA CCGGAAGAGG TACACCAGCA
4681 CCAAAGAGGT GCTGGACGCC ACCCTGATCC ACCAGAGCAT CACCGGCCTG TACGAGACAC
4741 GGATCGACCT GTCTCAGCTG GGAGGCGACA AAAGGCCGGC GGCCACGAAA AAGGCCGGCC
4801 AGGCAAAAAA GAAAAAGtaa GGATCCGGGG TTGGGGTTGC GCCTTTTCCA AGGCAGCCCT
4861 GGGTTTGCGC AGGGACGCGG CTGCTCTGGG CGTGGTTCCG GGAAACGCAG CGGCGCCGAC
4921 CCTGGGTCTC GCACATTCTT CACGTCCGTT CGCAGCGTCA CCCGGATCTT CGCCGCTACC
4981 CTTGTGGGCC CCCCGGCGAC GCTTCCTGCT CCGCCCCTAA GTCGGGAAGG TTCCTTGCGG
5041 TTCGCGGCGT GCCGGACGTG ACAAACGGAA GCCGCACGTC TCACTAGTAC CCTCGCAGAC
5101 GGACAGCGCC AGGGAGCAAT GGCAGCGCGC CGACCGCGAT GGGCTGTGGC CAATAGCGGC
5161 TGCTCAGCAG GGCGCGCCGA GAGCAGCGGC CGGGAAGGGG CGGTGCGGGA GGCGGGGTGT
5221 GGGGCGGTAG TGTGGGCCCT GTTCCTGCCC GCGCGGTGTT CCGCATTCTG CAAGCCTCCG
5281 GAGCGCACGT CGGCAGTCGG CTCCCTCGTT GACCGAATCA CCGACCTCTC TCCCCAGCAA
5341 TTCACCATGA CCGAGTACAA GCCCACGGTG CGCCTCGCCA CCCGCGACGA CGTCCCCAGG
5401 GCCGTACGCA CCCTCGCCGC CGCGTTCGCC GACTACCCCG CCACGCGCCA CACCGTCGAT
5461 CCGGACCGCC ACATCGAGCG GGTCACCGAG CTGCAAGAAC TCTTCCTCAC GCGCGTCGGG
5521 CTCGACATCG GCAAGGTGTG GGTCGCGGAC GACGGCGCCG CGGTGGCGGT CTGGACCACG
5581 CCGGAGAGCG TCGAAGCGGG GGCGGTGTTC GCCGAGATCG GCCCGCGCAT GGCCGAGTTG
5641 AGCGGTTCCC GGCTGGCCGC GCAGCAACAG ATGGAAGGCC TCCTGGCGCC GCACCGGCCC
5701 AAGGAGCCCG CGTGGTTCCT GGCCACCGTC GGCGTCTCGC CCGACCACCA GGGCAAGGGT
5761 CTGGGCAGCG CCGTCGTGCT CCCCGGAGTG GAGGCGGCCG AGCGCGCCGG GGTGCCCGCC
5821 TTCCTGGAGA CCTCCGCGCC CCGCAACCTC CCCTTCTACG AGCGGCTCGG CTTCACCGTC
5881 ACCGCCGACG TCGAGGTGCC CGAAGGACCG CGCACCTGGT GCATGACCCG CAAGCCCGGT
5941 GCCGAAGGTA GAGGTTCTCT CCTCACTTGT GGTGATGTTG AAGAAAACCC TGGTCCAATG
6001 TCTAGACTGG ACAAGAGCAA AGTCATAAAC GGAGCTCTGG AATTACTCAA TGGTGTCGGT
6061 ATCGAAGGCC TGACGACAAG GAAACTCGCT CAAAAGCTGG GAGTTGAGCA GCCTACCCTG
6121 TACTGGCACG TGAAGAACAA GCGGGCCCTG CTCGATGCCC TGCCAATCGA GATGCTGGAC
6181 AGGCATCATA CCCACTTCTG CCCCCTGGAA GGCGAGTCAT GGCAAGACTT TCTGCGGAAC
6241 AACGCCAAGT CATACCGCTG TGCTCTCCTC TCACATCGCG ACGGGGCTAA AGTGCATCTC
6301 GGCACCCGCC CAACAGAGAA ACAGTACGAA ACCCTGGAAA ATCAGCTCGC GTTCCTGTGT
6361 CAGCAAGGCT TCTCCCTGGA GAACGCACTG TACGCTCTGT CCGCCGTGGG CCACTTTACA
6421 CTGGGCTGCG TATTGGAGGA ACAGGAGCAT CAAGTAGCAA AAGAGGAAAG AGAGACACCT
6481 ACCACCGATT CTATGCCCCC ACTTCTGAGA CAAGCAATTG AGCTGTTCGA CCGGCAGGGA
6541 GCCGAACCTG CCTTCCTTTT CGGCCTGGAA CTAATCATAT GTGGCCTGGA GAAACAGCTA
6601 AAGTGCGAAA GCGGCGGGCC GACCGACGCC CTTGACGATT TTGACTTAGA CATGCTCCCA
6661 GCCGATGCCC TTGACGACTT TGACCTTGAT ATGCTGCCTG CTGACGCTCT TGACGATTTT
6721 GACCTTGACA TGCTCCCCGG GTAACTAAGT AAGGATCGAT CCAAGATATC GTATTCTTAA
6781 CTATGTTGCT CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT ATCATGCTAT
6841 TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC CTTGTATAAA TCCTGGTTGC TGTCTCTTTA
6901 TGAGGAGTTG TGGCCCGTTG TCAGGCAACG TGGCGTGGTG TGCACTGTGT TTGCTGACGC
6961 AACCCCCACT GGTTGGGGCA TTGCCACCAC CTGTCAGCTC CTTTCCGGGA CTTTCGCTTT
7021 CCCCCTCCCT ATTGCCACGG CGGAACTCAT CGCCGCCTGC CTTGCCCGCT GCTGGACAGG
7081 GGCTCGGCTG TTGGGCACTG ACAATTCCGT GGTGTTGTCG GGGAAGCTGA CGTCCTTTCC
7141 ATGGCTGCTC GCCTGTGTTG CCACCTGGAT TCTGCGCGGG ACGTCCTTCT GCTACGTCCC
7201 TTCGGCCCTC AATCCAGCGG ACCTTCCTTC CCGCGGCCTG CTGCCGGCTC TGCGGCCTCT
7261 TCCGCGTCTT CGCCTTCGCC CTCAGACGAG TCGGATCTCC CTTTGGGCCG CCTCCCCGCC
7321 TGTTTCGCCT CGGCGTCCGG ACTAGAGGTA CCTTTAAGAC CAATGACTTA CAAGGCAGCT
7381 GTAGATCTTA GCCACTTTTT AAAAGAAAAG GGGGGACTGG AAGGGCTAAT TCACTCCCAA
7441 CGAAGACAAG ATCTGCTTTT TGCTTGTACT GGGTCTCTCT GGTTAGACCA GATCTGAGCC
7501 TGGGAGCTCT CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG CTTGCCTTGA
7561 GTGCTTCAAG TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG ATCCCTCAGA
7621 CCCTTTTAGT CAGTGTGGAA AATCTCTAGC AGTAGTAGTT CATGTCATCT TATTATTCAG
7681 TATTTATAAC TTGCAAAGAA ATGAATATCA GAGAGTGAGA GGAACTTGTT TATTGCAGCT
7741 TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA
7801 CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGGCTCTAG
7861 CTATCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC
7921 CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG
7981 AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT TTCAAATATG TATCCGCTCA
8041 TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC
8101 AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC
8161 ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT
8221 ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT
8281 TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTATTGACG
8341 CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT
8401 CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG
8461 CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA
8521 AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG
8581 AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA
8641 TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC
8701 AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC
8761 CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA
8821 TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA
8881 GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA
8941 AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC
9001 ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC
9061 CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT
9121 CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC
9181 CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT
9241 TCAGCAGAGC GCAGATACCA AATACTGTTC TTCTAGTGTA GCCGTAGTTA GGCCACCACT
9301 TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG
9361 CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA
9421 AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA
9481 CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG
9541 GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG
9601 AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC
9661 TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA
9721 ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG
9781 CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC
9841 GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA
9901 TACGCAAACC GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT
9961 TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CTCACTCATT
10021 AGGCACCCCA GGCTTTACAC TTTATGCTTC CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG
10081 GATAACAATT TCACACAGGA AACAGCTATG ACCATGATTA CGCCAAGCGC GCAATTAACC
10141 CTCACTAAAG GGAACAAAAG CTGGAGCTGC AAGCTTAATG TAGTCTTATG CAATACTCTT
10201 GTAGTCTTGC AACATGGTAA CGATGAGTTA GCAACATGCC TTACAAGGAG AGAAAAAGCA
10261 CCGTGCATGC CGATTGGTGG AAGTAAGGTG GTACGATCGT GCCTTATTAG GAAGGCAACA
10321 GACGGGTCTG ACATGGATTG GACGAACCAC TGAATTGCCG CATTGCAGAG ATATTGTATT
10381 TAAGTGCCTA GCTCGATACA TAAACGGGTC TCTCTGGTTA GACCAGATCT GAGCCTGGGA
10441 GCTCTCTGGC TAACTAGGGA ACCCACTGCT TAAGCCTCAA TAAAGCTTGC CTTGAGTGCT
10501 TCAAGTAGTG TGTGCCCGTC TGTTGTGTGA CTCTGGTAAC TAGAGATCCC TCAGACCCTT
10561 TTAGTCAGTG TGGAAAATCT CTAGCAGTGG CGCCCGAACA GGGACTTGAA AGCGAAAGGG
10621 AAACCAGAGG AGCTCTCTCG ACGCAGGACT CGGCTTGCTG AAGCGCGCAC GGCAAGAGGC
10681 GAGGGGCGGC GACTGGTGAG TACGCCAAAA ATTTTGACTA GCGGAGGCTA GAAGGAGAGA
10741 GATGGGTGCG AGAGCGTCAG TATTAAGCGG GGGAGAATTA GATCGCGATG GGAAAAAATT
10801 CGGTTAAGGC CAGGGGGAAA GAAAAAATAT AAATTAAAAC ATATAGTATG GGCAAGCAGG
10861 GAGCTAGAAC GATTCGCAGT TAATCCTGGC CTGTTAGAAA CATCAGAAGG CTGTAGACAA
10921 ATACTGGGAC AGCTACAACC ATCCCTTCAG ACAGGATCAG AAGAACTTAG ATCATTATAT
10981 AATACAGTAG CAACCCTCTA TTGTGTGCAT CAAAGGATAG AGATAAAAGA CACCAAGGAA
11041 GCTTTAGACA AGATAGAGGA AGAGCAAAAC AAAAGTAAGA CCACCGCACA GCAAGCGGCC
11101 GCTGATCTTC AGACCTGGAG GAGGAGATAT GAGGGACAAT TGGAGAAGTG AATTATATAA
11161 ATATAAAGTA GTAAAAATTG AACCATTAGG AGTAGCACCC ACCAAGGCAA AGAGAAGAGT
11221 GGTGCAGAGA GAAAAAAGAG CAGTGGGAAT AGGAGCTTTG TTCCTTGGGT TCTTGGGAGC
11281 AGCAGGAAGC ACTATGGGCG CAGCGTCAAT GACGCTGACG GTACAGGCCA GACAATTATT
11341 GTCTGGTATA GTGCAGCAGC AGAACAATTT GCTGAGGGCT ATTGAGGCGC AACAGCATCT
11401 GTTGCAACTC ACAGTCTGGG GCATCAAGCA GCTCCAGGCA AGAATCCTGG CTGTGGAAAG
11461 ATACCTAAAG GATCAACAGC TCCTGGGGAT TTGGGGTTGC TCTGGAAAAC TCATTTGCAC
11521 CACTGCTGTG CCTTGGAATG CTAGTTGGAG TAATAAATCT CTGGAACAGA TTTGGAATCA
11581 CACGACCTGG ATGGAGTGGG ACAGAGAAAT TAACAATTAC ACAAGCTTAA TACACTCCTT
11641 AATTGAAGAA TCGCAAAACC AGCAAGAAAA GAATGAACAA GAATTATTGG AATTAGATAA
11701 ATGGGCAAGT TTGTGGAATT GGTTTAACAT AACAAATTGG CTGTGGTATA TAAAATTATT
11761 CATAATGATA GTAGGAGGCT TGGTAGGTTT AAGAATAGTT TTTGCTGTAC TTTCTATAGT
11821 GAATAGAGTT AGGCAGGGAT ATTCACCATT ATCGTTTCAG ACCCACCTCC CAACCCCGAG
11881 GGGAC
//
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