pCW-Cas9

价格:1200元

联系方式:I47-825O-882O

相关技术服务:质粒构建    基因合成    质粒大提

买家导航

载体基本信息

质粒名称: pCW-Cas9 
出品公司: 爱迪基因
目录编号: 50661
存储实验室: Eric Lander, David Sabatini 

载体骨架基本信息
载体名称: pCW57.1
载体抗性: 氨苄霉素
空载体大小: 7600 bp
完整质粒大小: 11900 bp
载体类型: 哺乳细胞表达,慢病毒载体,CRISPR
筛选标记: Puromycin
高拷贝/低拷贝: 高拷贝
生长条件: 37 ℃

插入基因信息
插入基因名称: humanized S. pyogenes Cas9
插入大小: 4300 bp
启动子 Tet ON
标签/融合蛋白: 3xFLAG(N端)
克隆方法: 限制性内切酶
5' 克隆位点: NheI(未破坏)
3' 克隆位点: BamHI (未破坏)
5' 测序引物 AGCTCGTTTAGTGAACCGTCAGATC
3' 测序引物 CACATTCTTCACGTCCGTTC

文献引用方法
材料和方法部分: pCW-Cas9 was a gift from Eric Lander & David Sabatini (Addgene plasmid # 50661)
参考文献部分: Genetic screens in human cells using the CRISPR-Cas9 system. Wang T, Wei JJ, Sabatini DM, Lander ES. Science. 2014 Jan 3;343(6166):80-4. doi: 10.1126/science.1246981. Epub 2013 Dec 12. 10.1126/science.1246981 PubMed 24336569

载体图谱质粒图谱和多克隆位点信息

pCW-Cas9载体图谱

载体简介

载体序列

LOCUS       pCW-Cas9 Sequencing Result	11885 bp 	DNA	SYN
DEFINITION  pCW-Cas9 Sequencing Result
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..11885
                     /organism="pCW-Cas9 Sequencing Result"
                     /mol_type="other DNA"
     misc_feature    45..60
                     /label="cPPT"
     CDS             complement(369..5348)
                     /label="ORF frame 1"
     misc_feature    497..516
                     /label="pCEP_fwd_primer"
     misc_feature    499..523
                     /label="LNCX_primer"
     CDS             549..4820
                     /label="ORF frame 3"
     misc_feature    552..572
                     /label="FLAG"
     misc_feature    573..593
                     /label="FLAG"
     misc_feature    594..617
                     /label="FLAG"
     misc_feature    624..644
                     /label="SV40 NLS"
     misc_feature    624..647
                     /label="SV40 NLS"
     misc_feature    630..644
                     /label="NLS"
     CDS             5119..6744
                     /label="ORF frame 1"
     gene            5347..5934
                     /label="puro (variant)"
                     /gene="puro (variant)"
     misc_feature    6781..7308
                     /label="WPRE"
     CDS             6821..7345
                     /label="ORF frame 2"
     misc_feature    7401..7416
                     /label="cPPT"
     misc_feature    7401..7422
                     /label="U3PPT"
     misc_feature    7418..7470
                     /label="delta_U3"
     misc_feature    7471..7651
                     /label="truncHIV-1_3_LTR"
     misc_feature    7471..7651
                     /label="HIV-1_5_LTR"
     terminator      7726..7845
                     /label="SV40_PA_terminator"
     misc_feature    7814..7833
                     /label="EBV_rev_primer"
     rep_origin      7863..7961
                     /label="SV40_origin"
     misc_feature    7946..7965
                     /label="SV40pro_F_primer"
     promoter        8021..8049
                     /label="AmpR_promoter"
     gene            8091..8951
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             8091..8951
                     /label="ORF frame 3"
     rep_origin      9106..9725
                     /label="pBR322_origin"
     promoter        10034..10063
                     /label="lac_promoter"
     misc_feature    10077..10099
                     /label="M13_pUC_rev_primer"
     promoter        10098..10116
                     /label="M13_reverse_primer"
     promoter        10133..10152
                     /label="T3_promoter"
     misc_feature    10406..10586
                     /label="truncHIV-1_3_LTR"
     misc_feature    10406..10586
                     /label="HIV-1_5_LTR"
     misc_feature    10697..10741
                     /label="HIV-1_psi_pack"
     CDS             11129..410
                     /label="ORF frame 2"
     misc_feature    11251..11484
                     /label="RRE"
ORIGIN
    1 AATTCTCGAC CTCGAGACAA ATGGCAGTAT TCATCCACAA TTTTAAAAGA AAAGGGGGGA
   61 TTGGGGGGTA CAGTGCAGGG GAAAGAATAG TAGACATAAT AGCAACAGAC ATACAAACTA
  121 AAGAATTACA AAAACAAATT ACAAAAATTC AAAATTTTCG GGTTTATTAC AGGGACAGCA
  181 GAGATCCACT TTGGCCGCGA ATCGATATGT CGAGTTTACT CCCTATCAGT GATAGAGAAC
  241 GTATGTCGAG TTTACTCCCT ATCAGTGATA GAGAACGATG TCGAGTTTAC TCCCTATCAG
  301 TGATAGAGAA CGTATGTCGA GTTTACTCCC TATCAGTGAT AGAGAACGTA TGTCGAGTTT
  361 ACTCCCTATC AGTGATAGAG AACGTATGTC GAGTTTATCC CTATCAGTGA TAGAGAACGT
  421 ATGTCGAGTT TACTCCCTAT CAGTGATAGA GAACGTATGT CGAGGTAGGC GTGTACGGTG
  481 GGAGGCCTAT ATAAGCAGAG CTCGTTTAGT GAACCGTCAG ATCGCCTGGA GAATTGGCTA
  541 GCgccaccAT GGACTATAAG GACCACGACG GAGACTACAA GGATCATGAT ATTGATTACA
  601 AAGACGATGA CGATAAGATG GCCCCAAAGA AGAAGCGGAA GGTCGGTATC CACGGAGTCC
  661 CAGCAGCCGA CAAGAAGTAC AGCATCGGCC TGGACATCGG CACCAACTCT GTGGGCTGGG
  721 CCGTGATCAC CGACGAGTAC AAGGTGCCCA GCAAGAAATT CAAGGTGCTG GGCAACACCG
  781 ACCGGCACAG CATCAAGAAG AACCTGATCG GAGCCCTGCT GTTCGACAGC GGCGAAACAG
  841 CCGAGGCCAC CCGGCTGAAG AGAACCGCCA GAAGAAGATA CACCAGACGG AAGAACCGGA
  901 TCTGCTATCT GCAAGAGATC TTCAGCAACG AGATGGCCAA GGTGGACGAC AGCTTCTTCC
  961 ACAGACTGGA AGAGTCCTTC CTGGTGGAAG AGGATAAGAA GCACGAGCGG CACCCCATCT
 1021 TCGGCAACAT CGTGGACGAG GTGGCCTACC ACGAGAAGTA CCCCACCATC TACCACCTGA
 1081 GAAAGAAACT GGTGGACAGC ACCGACAAGG CCGACCTGCG GCTGATCTAT CTGGCCCTGG
 1141 CCCACATGAT CAAGTTCCGG GGCCACTTCC TGATCGAGGG CGACCTGAAC CCCGACAACA
 1201 GCGACGTGGA CAAGCTGTTC ATCCAGCTGG TGCAGACCTA CAACCAGCTG TTCGAGGAAA
 1261 ACCCCATCAA CGCCAGCGGC GTGGACGCCA AGGCCATCCT GTCTGCCAGA CTGAGCAAGA
 1321 GCAGACGGCT GGAAAATCTG ATCGCCCAGC TGCCCGGCGA GAAGAAGAAT GGCCTGTTCG
 1381 GAAACCTGAT TGCCCTGAGC CTGGGCCTGA CCCCCAACTT CAAGAGCAAC TTCGACCTGG
 1441 CCGAGGATGC CAAACTGCAG CTGAGCAAGG ACACCTACGA CGACGACCTG GACAACCTGC
 1501 TGGCCCAGAT CGGCGACCAG TACGCCGACC TGTTTCTGGC CGCCAAGAAC CTGTCCGACG
 1561 CCATCCTGCT GAGCGACATC CTGAGAGTGA ACACCGAGAT CACCAAGGCC CCCCTGAGCG
 1621 CCTCTATGAT CAAGAGATAC GACGAGCACC ACCAGGACCT GACCCTGCTG AAAGCTCTCG
 1681 TGCGGCAGCA GCTGCCTGAG AAGTACAAAG AGATTTTCTT CGACCAGAGC AAGAACGGCT
 1741 ACGCCGGCTA CATTGACGGC GGAGCCAGCC AGGAAGAGTT CTACAAGTTC ATCAAGCCCA
 1801 TCCTGGAAAA GATGGACGGC ACCGAGGAAC TGCTCGTGAA GCTGAACAGA GAGGACCTGC
 1861 TGCGGAAGCA GCGGACCTTC GACAACGGCA GCATCCCCCA CCAGATCCAC CTGGGAGAGC
 1921 TGCACGCCAT TCTGCGGCGG CAGGAAGATT TTTACCCATT CCTGAAGGAC AACCGGGAAA
 1981 AGATCGAGAA GATCCTGACC TTCCGCATCC CCTACTACGT GGGCCCTCTG GCCAGGGGAA
 2041 ACAGCAGATT CGCCTGGATG ACCAGAAAGA GCGAGGAAAC CATCACCCCC TGGAACTTCG
 2101 AGGAAGTGGT GGACAAGGGC GCTTCCGCCC AGAGCTTCAT CGAGCGGATG ACCAACTTCG
 2161 ATAAGAACCT GCCCAACGAG AAGGTGCTGC CCAAGCACAG CCTGCTGTAC GAGTACTTCA
 2221 CCGTGTATAA CGAGCTGACC AAAGTGAAAT ACGTGACCGA GGGAATGAGA AAGCCCGCCT
 2281 TCCTGAGCGG CGAGCAGAAA AAGGCCATCG TGGACCTGCT GTTCAAGACC AACCGGAAAG
 2341 TGACCGTGAA GCAGCTGAAA GAGGACTACT TCAAGAAAAT CGAGTGCTTC GACTCCGTGG
 2401 AAATCTCCGG CGTGGAAGAT CGGTTCAACG CCTCCCTGGG CACATACCAC GATCTGCTGA
 2461 AAATTATCAA GGACAAGGAC TTCCTGGACA ATGAGGAAAA CGAGGACATT CTGGAAGATA
 2521 TCGTGCTGAC CCTGACACTG TTTGAGGACA GAGAGATGAT CGAGGAACGG CTGAAAACCT
 2581 ATGCCCACCT GTTCGACGAC AAAGTGATGA AGCAGCTGAA GCGGCGGAGA TACACCGGCT
 2641 GGGGCAGGCT GAGCCGGAAG CTGATCAACG GCATCCGGGA CAAGCAGTCC GGCAAGACAA
 2701 TCCTGGATTT CCTGAAGTCC GACGGCTTCG CCAACAGAAA CTTCATGCAG CTGATCCACG
 2761 ACGACAGCCT GACCTTTAAA GAGGACATCC AGAAAGCCCA GGTGTCCGGC CAGGGCGATA
 2821 GCCTGCACGA GCACATTGCC AATCTGGCCG GCAGCCCCGC CATTAAGAAG GGCATCCTGC
 2881 AGACAGTGAA GGTGGTGGAC GAGCTCGTGA AAGTGATGGG CCGGCACAAG CCCGAGAACA
 2941 TCGTGATCGA AATGGCCAGA GAGAACCAGA CCACCCAGAA GGGACAGAAG AACAGCCGCG
 3001 AGAGAATGAA GCGGATCGAA GAGGGCATCA AAGAGCTGGG CAGCCAGATC CTGAAAGAAC
 3061 ACCCCGTGGA AAACACCCAG CTGCAGAACG AGAAGCTGTA CCTGTACTAC CTGCAGAATG
 3121 GGCGGGATAT GTACGTGGAC CAGGAACTGG ACATCAACCG GCTGTCCGAC TACGATGTGG
 3181 ACCATATCGT GCCTCAGAGC TTTCTGAAGG ACGACTCCAT CGACAACAAG GTGCTGACCA
 3241 GAAGCGACAA GAACCGGGGC AAGAGCGACA ACGTGCCCTC CGAAGAGGTC GTGAAGAAGA
 3301 TGAAGAACTA CTGGCGGCAG CTGCTGAACG CCAAGCTGAT TACCCAGAGA AAGTTCGACA
 3361 ATCTGACCAA GGCCGAGAGA GGCGGCCTGA GCGAACTGGA TAAGGCCGGC TTCATCAAGA
 3421 GACAGCTGGT GGAAACCCGG CAGATCACAA AGCACGTGGC ACAGATCCTG GACTCCCGGA
 3481 TGAACACTAA GTACGACGAG AATGACAAGC TGATCCGGGA AGTGAAAGTG ATCACCCTGA
 3541 AGTCCAAGCT GGTGTCCGAT TTCCGGAAGG ATTTCCAGTT TTACAAAGTG CGCGAGATCA
 3601 ACAACTACCA CCACGCCCAC GACGCCTACC TGAACGCCGT CGTGGGAACC GCCCTGATCA
 3661 AAAAGTACCC TAAGCTGGAA AGCGAGTTCG TGTACGGCGA CTACAAGGTG TACGACGTGC
 3721 GGAAGATGAT CGCCAAGAGC GAGCAGGAAA TCGGCAAGGC TACCGCCAAG TACTTCTTCT
 3781 ACAGCAACAT CATGAACTTT TTCAAGACCG AGATTACCCT GGCCAACGGC GAGATCCGGA
 3841 AGCGGCCTCT GATCGAGACA AACGGCGAAA CCGGGGAGAT CGTGTGGGAT AAGGGCCGGG
 3901 ATTTTGCCAC CGTGCGGAAA GTGCTGAGCA TGCCCCAAGT GAATATCGTG AAAAAGACCG
 3961 AGGTGCAGAC AGGCGGCTTC AGCAAAGAGT CTATCCTGCC CAAGAGGAAC AGCGATAAGC
 4021 TGATCGCCAG AAAGAAGGAC TGGGACCCTA AGAAGTACGG CGGCTTCGAC AGCCCCACCG
 4081 TGGCCTATTC TGTGCTGGTG GTGGCCAAAG TGGAAAAGGG CAAGTCCAAG AAACTGAAGA
 4141 GTGTGAAAGA GCTGCTGGGG ATCACCATCA TGGAAAGAAG CAGCTTCGAG AAGAATCCCA
 4201 TCGACTTTCT GGAAGCCAAG GGCTACAAAG AAGTGAAAAA GGACCTGATC ATCAAGCTGC
 4261 CTAAGTACTC CCTGTTCGAG CTGGAAAACG GCCGGAAGAG AATGCTGGCC TCTGCCGGCG
 4321 AACTGCAGAA GGGAAACGAA CTGGCCCTGC CCTCCAAATA TGTGAACTTC CTGTACCTGG
 4381 CCAGCCACTA TGAGAAGCTG AAGGGCTCCC CCGAGGATAA TGAGCAGAAA CAGCTGTTTG
 4441 TGGAACAGCA CAAGCACTAC CTGGACGAGA TCATCGAGCA GATCAGCGAG TTCTCCAAGA
 4501 GAGTGATCCT GGCCGACGCT AATCTGGACA AAGTGCTGTC CGCCTACAAC AAGCACCGGG
 4561 ATAAGCCCAT CAGAGAGCAG GCCGAGAATA TCATCCACCT GTTTACCCTG ACCAATCTGG
 4621 GAGCCCCTGC CGCCTTCAAG TACTTTGACA CCACCATCGA CCGGAAGAGG TACACCAGCA
 4681 CCAAAGAGGT GCTGGACGCC ACCCTGATCC ACCAGAGCAT CACCGGCCTG TACGAGACAC
 4741 GGATCGACCT GTCTCAGCTG GGAGGCGACA AAAGGCCGGC GGCCACGAAA AAGGCCGGCC
 4801 AGGCAAAAAA GAAAAAGtaa GGATCCGGGG TTGGGGTTGC GCCTTTTCCA AGGCAGCCCT
 4861 GGGTTTGCGC AGGGACGCGG CTGCTCTGGG CGTGGTTCCG GGAAACGCAG CGGCGCCGAC
 4921 CCTGGGTCTC GCACATTCTT CACGTCCGTT CGCAGCGTCA CCCGGATCTT CGCCGCTACC
 4981 CTTGTGGGCC CCCCGGCGAC GCTTCCTGCT CCGCCCCTAA GTCGGGAAGG TTCCTTGCGG
 5041 TTCGCGGCGT GCCGGACGTG ACAAACGGAA GCCGCACGTC TCACTAGTAC CCTCGCAGAC
 5101 GGACAGCGCC AGGGAGCAAT GGCAGCGCGC CGACCGCGAT GGGCTGTGGC CAATAGCGGC
 5161 TGCTCAGCAG GGCGCGCCGA GAGCAGCGGC CGGGAAGGGG CGGTGCGGGA GGCGGGGTGT
 5221 GGGGCGGTAG TGTGGGCCCT GTTCCTGCCC GCGCGGTGTT CCGCATTCTG CAAGCCTCCG
 5281 GAGCGCACGT CGGCAGTCGG CTCCCTCGTT GACCGAATCA CCGACCTCTC TCCCCAGCAA
 5341 TTCACCATGA CCGAGTACAA GCCCACGGTG CGCCTCGCCA CCCGCGACGA CGTCCCCAGG
 5401 GCCGTACGCA CCCTCGCCGC CGCGTTCGCC GACTACCCCG CCACGCGCCA CACCGTCGAT
 5461 CCGGACCGCC ACATCGAGCG GGTCACCGAG CTGCAAGAAC TCTTCCTCAC GCGCGTCGGG
 5521 CTCGACATCG GCAAGGTGTG GGTCGCGGAC GACGGCGCCG CGGTGGCGGT CTGGACCACG
 5581 CCGGAGAGCG TCGAAGCGGG GGCGGTGTTC GCCGAGATCG GCCCGCGCAT GGCCGAGTTG
 5641 AGCGGTTCCC GGCTGGCCGC GCAGCAACAG ATGGAAGGCC TCCTGGCGCC GCACCGGCCC
 5701 AAGGAGCCCG CGTGGTTCCT GGCCACCGTC GGCGTCTCGC CCGACCACCA GGGCAAGGGT
 5761 CTGGGCAGCG CCGTCGTGCT CCCCGGAGTG GAGGCGGCCG AGCGCGCCGG GGTGCCCGCC
 5821 TTCCTGGAGA CCTCCGCGCC CCGCAACCTC CCCTTCTACG AGCGGCTCGG CTTCACCGTC
 5881 ACCGCCGACG TCGAGGTGCC CGAAGGACCG CGCACCTGGT GCATGACCCG CAAGCCCGGT
 5941 GCCGAAGGTA GAGGTTCTCT CCTCACTTGT GGTGATGTTG AAGAAAACCC TGGTCCAATG
 6001 TCTAGACTGG ACAAGAGCAA AGTCATAAAC GGAGCTCTGG AATTACTCAA TGGTGTCGGT
 6061 ATCGAAGGCC TGACGACAAG GAAACTCGCT CAAAAGCTGG GAGTTGAGCA GCCTACCCTG
 6121 TACTGGCACG TGAAGAACAA GCGGGCCCTG CTCGATGCCC TGCCAATCGA GATGCTGGAC
 6181 AGGCATCATA CCCACTTCTG CCCCCTGGAA GGCGAGTCAT GGCAAGACTT TCTGCGGAAC
 6241 AACGCCAAGT CATACCGCTG TGCTCTCCTC TCACATCGCG ACGGGGCTAA AGTGCATCTC
 6301 GGCACCCGCC CAACAGAGAA ACAGTACGAA ACCCTGGAAA ATCAGCTCGC GTTCCTGTGT
 6361 CAGCAAGGCT TCTCCCTGGA GAACGCACTG TACGCTCTGT CCGCCGTGGG CCACTTTACA
 6421 CTGGGCTGCG TATTGGAGGA ACAGGAGCAT CAAGTAGCAA AAGAGGAAAG AGAGACACCT
 6481 ACCACCGATT CTATGCCCCC ACTTCTGAGA CAAGCAATTG AGCTGTTCGA CCGGCAGGGA
 6541 GCCGAACCTG CCTTCCTTTT CGGCCTGGAA CTAATCATAT GTGGCCTGGA GAAACAGCTA
 6601 AAGTGCGAAA GCGGCGGGCC GACCGACGCC CTTGACGATT TTGACTTAGA CATGCTCCCA
 6661 GCCGATGCCC TTGACGACTT TGACCTTGAT ATGCTGCCTG CTGACGCTCT TGACGATTTT
 6721 GACCTTGACA TGCTCCCCGG GTAACTAAGT AAGGATCGAT CCAAGATATC GTATTCTTAA
 6781 CTATGTTGCT CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT ATCATGCTAT
 6841 TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC CTTGTATAAA TCCTGGTTGC TGTCTCTTTA
 6901 TGAGGAGTTG TGGCCCGTTG TCAGGCAACG TGGCGTGGTG TGCACTGTGT TTGCTGACGC
 6961 AACCCCCACT GGTTGGGGCA TTGCCACCAC CTGTCAGCTC CTTTCCGGGA CTTTCGCTTT
 7021 CCCCCTCCCT ATTGCCACGG CGGAACTCAT CGCCGCCTGC CTTGCCCGCT GCTGGACAGG
 7081 GGCTCGGCTG TTGGGCACTG ACAATTCCGT GGTGTTGTCG GGGAAGCTGA CGTCCTTTCC
 7141 ATGGCTGCTC GCCTGTGTTG CCACCTGGAT TCTGCGCGGG ACGTCCTTCT GCTACGTCCC
 7201 TTCGGCCCTC AATCCAGCGG ACCTTCCTTC CCGCGGCCTG CTGCCGGCTC TGCGGCCTCT
 7261 TCCGCGTCTT CGCCTTCGCC CTCAGACGAG TCGGATCTCC CTTTGGGCCG CCTCCCCGCC
 7321 TGTTTCGCCT CGGCGTCCGG ACTAGAGGTA CCTTTAAGAC CAATGACTTA CAAGGCAGCT
 7381 GTAGATCTTA GCCACTTTTT AAAAGAAAAG GGGGGACTGG AAGGGCTAAT TCACTCCCAA
 7441 CGAAGACAAG ATCTGCTTTT TGCTTGTACT GGGTCTCTCT GGTTAGACCA GATCTGAGCC
 7501 TGGGAGCTCT CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG CTTGCCTTGA
 7561 GTGCTTCAAG TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG ATCCCTCAGA
 7621 CCCTTTTAGT CAGTGTGGAA AATCTCTAGC AGTAGTAGTT CATGTCATCT TATTATTCAG
 7681 TATTTATAAC TTGCAAAGAA ATGAATATCA GAGAGTGAGA GGAACTTGTT TATTGCAGCT
 7741 TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA
 7801 CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGGCTCTAG
 7861 CTATCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC
 7921 CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG
 7981 AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT TTCAAATATG TATCCGCTCA
 8041 TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC
 8101 AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC
 8161 ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT
 8221 ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT
 8281 TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTATTGACG
 8341 CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT
 8401 CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG
 8461 CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA
 8521 AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG
 8581 AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA
 8641 TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC
 8701 AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC
 8761 CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA
 8821 TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA
 8881 GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA
 8941 AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC
 9001 ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC
 9061 CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT
 9121 CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC
 9181 CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT
 9241 TCAGCAGAGC GCAGATACCA AATACTGTTC TTCTAGTGTA GCCGTAGTTA GGCCACCACT
 9301 TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG
 9361 CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA
 9421 AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA
 9481 CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG
 9541 GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG
 9601 AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC
 9661 TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA
 9721 ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG
 9781 CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC
 9841 GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA
 9901 TACGCAAACC GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT
 9961 TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CTCACTCATT
10021 AGGCACCCCA GGCTTTACAC TTTATGCTTC CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG
10081 GATAACAATT TCACACAGGA AACAGCTATG ACCATGATTA CGCCAAGCGC GCAATTAACC
10141 CTCACTAAAG GGAACAAAAG CTGGAGCTGC AAGCTTAATG TAGTCTTATG CAATACTCTT
10201 GTAGTCTTGC AACATGGTAA CGATGAGTTA GCAACATGCC TTACAAGGAG AGAAAAAGCA
10261 CCGTGCATGC CGATTGGTGG AAGTAAGGTG GTACGATCGT GCCTTATTAG GAAGGCAACA
10321 GACGGGTCTG ACATGGATTG GACGAACCAC TGAATTGCCG CATTGCAGAG ATATTGTATT
10381 TAAGTGCCTA GCTCGATACA TAAACGGGTC TCTCTGGTTA GACCAGATCT GAGCCTGGGA
10441 GCTCTCTGGC TAACTAGGGA ACCCACTGCT TAAGCCTCAA TAAAGCTTGC CTTGAGTGCT
10501 TCAAGTAGTG TGTGCCCGTC TGTTGTGTGA CTCTGGTAAC TAGAGATCCC TCAGACCCTT
10561 TTAGTCAGTG TGGAAAATCT CTAGCAGTGG CGCCCGAACA GGGACTTGAA AGCGAAAGGG
10621 AAACCAGAGG AGCTCTCTCG ACGCAGGACT CGGCTTGCTG AAGCGCGCAC GGCAAGAGGC
10681 GAGGGGCGGC GACTGGTGAG TACGCCAAAA ATTTTGACTA GCGGAGGCTA GAAGGAGAGA
10741 GATGGGTGCG AGAGCGTCAG TATTAAGCGG GGGAGAATTA GATCGCGATG GGAAAAAATT
10801 CGGTTAAGGC CAGGGGGAAA GAAAAAATAT AAATTAAAAC ATATAGTATG GGCAAGCAGG
10861 GAGCTAGAAC GATTCGCAGT TAATCCTGGC CTGTTAGAAA CATCAGAAGG CTGTAGACAA
10921 ATACTGGGAC AGCTACAACC ATCCCTTCAG ACAGGATCAG AAGAACTTAG ATCATTATAT
10981 AATACAGTAG CAACCCTCTA TTGTGTGCAT CAAAGGATAG AGATAAAAGA CACCAAGGAA
11041 GCTTTAGACA AGATAGAGGA AGAGCAAAAC AAAAGTAAGA CCACCGCACA GCAAGCGGCC
11101 GCTGATCTTC AGACCTGGAG GAGGAGATAT GAGGGACAAT TGGAGAAGTG AATTATATAA
11161 ATATAAAGTA GTAAAAATTG AACCATTAGG AGTAGCACCC ACCAAGGCAA AGAGAAGAGT
11221 GGTGCAGAGA GAAAAAAGAG CAGTGGGAAT AGGAGCTTTG TTCCTTGGGT TCTTGGGAGC
11281 AGCAGGAAGC ACTATGGGCG CAGCGTCAAT GACGCTGACG GTACAGGCCA GACAATTATT
11341 GTCTGGTATA GTGCAGCAGC AGAACAATTT GCTGAGGGCT ATTGAGGCGC AACAGCATCT
11401 GTTGCAACTC ACAGTCTGGG GCATCAAGCA GCTCCAGGCA AGAATCCTGG CTGTGGAAAG
11461 ATACCTAAAG GATCAACAGC TCCTGGGGAT TTGGGGTTGC TCTGGAAAAC TCATTTGCAC
11521 CACTGCTGTG CCTTGGAATG CTAGTTGGAG TAATAAATCT CTGGAACAGA TTTGGAATCA
11581 CACGACCTGG ATGGAGTGGG ACAGAGAAAT TAACAATTAC ACAAGCTTAA TACACTCCTT
11641 AATTGAAGAA TCGCAAAACC AGCAAGAAAA GAATGAACAA GAATTATTGG AATTAGATAA
11701 ATGGGCAAGT TTGTGGAATT GGTTTAACAT AACAAATTGG CTGTGGTATA TAAAATTATT
11761 CATAATGATA GTAGGAGGCT TGGTAGGTTT AAGAATAGTT TTTGCTGTAC TTTCTATAGT
11821 GAATAGAGTT AGGCAGGGAT ATTCACCATT ATCGTTTCAG ACCCACCTCC CAACCCCGAG
11881 GGGAC
//

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