pMonAID_nCas9-PmCDA_Hyg_ALS

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联系方式:I47-825O-882O

相关技术服务:质粒构建    基因合成    质粒大提

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载体基本信息

质粒名称: pMonAID_nCas9-PmCDA_Hyg_ALS 
出品公司: 爱迪基因
目录编号: 91693
存储实验室: Akihiko Kondo

载体骨架基本信息
载体名称: pZDgRNA_Cas9ver.2 (D10A)_HPT
载体出品公司: --
载体抗性: 大观霉素
空载体大小: 17773 bp
完整质粒大小: --
载体修饰: --
载体类型: 植物表达
筛选标记: 潮霉素
高拷贝/低拷贝: --
生长条件: 37 ℃

插入基因信息
插入基因名称: SpCas9
物种来源: Streptococcus pyogenes
插入大小: --
基因突变: D10A for nickase Cas9
GenBank编号 --
启动子 2x35S
标签/融合蛋白: PmCDA1(插接件上的C端子)
克隆方法: Gibson克隆
5' 克隆位点: --
3' 克隆位点: --
5' 测序引物 ggaagttcatttcatttggagag
3' 测序引物 ccatttgcattttgatgtccg

文献引用方法
材料和方法部分: pMonAID_nCas9-PmCDA_Hyg_ALS was a gift from Akihiko Kondo (Addgene plasmid # 91693 ; http://n2t.net/addgene:91693 ; RRID:Addgene_91693)
参考文献部分: Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion. Shimatani Z, Kashojiya S, Takayama M, Terada R, Arazoe T, Ishii H, Teramura H, Yamamoto T, Komatsu H, Miura K, Ezura H, Nishida K, Ariizumi T, Kondo A. Nat Biotechnol. 2017 Mar 27. doi: 10.1038/nbt.3833. 10.1038/nbt.3833 PubMed 28346401

载体图谱质粒图谱和多克隆位点信息

pMonAID_nCas9-PmCDA_Hyg_ALS质粒图谱

载体简介

载体序列

LOCUS      pMonAID_nCas9-PmCDA_Hyg_ALS               19008 bp ds-DNA     circular SYN 06-AUG-2018
DEFINITION  Monocot Target-AID vector expressing rice-optimized nCas9-PmCDA1 
            with sgRNA targeting OsAls.
ACCESSION   .
VERSION     .
KEYWORDS    pMonAID_nCas9-PmCDA_Hyg_ALS
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19008)
  AUTHORS   Shimatani Z, Kashojiya S, Takayama M, Terada R, Arazoe T, Ishii H, 
            Teramura H, Yamamoto T, Komatsu H, Miura K, Ezura H, Nishida K, 
            Ariizumi T, Kondo A
  TITLE     Targeted base editing in rice and tomato using a CRISPR-Cas9 
            cytidine deaminase fusion.
  JOURNAL   Nat Biotechnol. 2017 Mar 27. doi: 10.1038/nbt.3833.
  PUBMED    28346401
REFERENCE   2  (bases 1 to 19008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..19008
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    113..137
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    295..316
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        331..361
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    369..385
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     374..396
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     393..409
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     393..409
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    485..509
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(559..575)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(560..576)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    596..695
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_RNA        1104..1179
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(1191..1215)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1221..1320)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        2111..2156
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     5'UTR           2167..2267
                     /label=OsADH 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the Oryza 
                     sativa alcohol dehydrogenase gene (Sugio et al., 2008)"
     CDS             2277..2297
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2322..6425
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6447..6467
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6660..6725
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             6732..7355
                     /codon_start=1
                     /product="cytidine deaminase from the sea lamprey 
                     Petromyzon marinus (Rogozin et al., 2007)"
                     /label=PmCDA1
                     /translation="MTDAEYVRIHEKLDIYTFKKQFFNNKKSVSHRCYVLFELKRRGER
                     RACFWGYAVNKPQSGTERGIHAEIFSIRKVEEYLRDNPGQFTINWYSSWSPCADCAEKI
                     LEWYNQELRGNGHTLKIWACKLYYEKNARNQIGLWNLRDNGVGLNVMVSEHYQCCRKIF
                     IQSSHNQLNENRWLEKTLKRAEKRRSELSIMIQVKILHTTKSPAV"
     CDS             7359..7379
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        9657..10002
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             10017..11042
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     protein_bind    complement(12194..12318)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(12358..12375)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(12358..12374)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12367..12389)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    12589..12613
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             13134..13925
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      14171..14759
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14660..14679
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14913..14930
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    14945..15085
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(15011..15030)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     15171..15193
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      15429..15623
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(15689..16762)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(17191..17820)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gatcccgagg ggaaccctgt ggttggcatg cacatacaaa tggacgaacg gataaacctt
       61 ttcacgccct tttaaatatc cgattattct aataaacgct cttttctctt aggtttaccc
      121 gccaatatat cctgtcaaac actgatagtt taaactgaag gcgggaaacg acaatctgat
      181 ccaagctcaa gctccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
      241 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
      301 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
      361 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa
      421 tttcaggtga ctgatagtga cctgttcgtt gcaacaaatt gatgagcaat gcttttttat
      481 aatgccaact ttgtacaaaa aagcaggcgg cgcgtcctgc aggataggga taacagggta
      541 atggcgcgcc aagcttatcg ataccgtcga cctcgagcgg ccgcgggaat tcgatcaaat
      601 aatgatttta ttttgactga tagtgacctg ttcgttgcaa caaattgatg agcaatgctt
      661 ttttataatg ccaactttgt acaaaaaagc aggctctttt tttcttcttc ttcgttcata
      721 cagttttttt ttgtttatca gcttacattt tcttgaaccg tagctttcgt tttcttcttt
      781 ttaactttcc attcggagtt tttgtatctt gtttcatagt ttgtcccagg attagaatga
      841 ttaggcatcg aaccttcaag aatttgattg aataaaacat cttcattctt aagatatgaa
      901 gataatcttc aaaaggcccc tgggaatctg aaagaagaga agcaggccca tttatatggg
      961 aaagaacaat agtatttctt atataggccc atttaagttg aaaacaatct tcaaaagtcc
     1021 cacatcgctt agataagaaa acgaagctga gtttatatac agctagagtc gaagtagtga
     1081 ttgcgcgtcc atggagatcc accgttttag agctagaaat agcaagttaa aataaggcta
     1141 gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttctag acccagcttt
     1201 cttgtacaaa gttggcatta acccagcttt cttgtacaaa gttggcatta taaaaaataa
     1261 ttgctcatca atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg
     1321 atcactagtg gatccgagct cggtacctta attaaattac cctgttatcc ctatcctgca
     1381 ggattaacac ccaacttttc tatccaagct tgccaacatg gtggagcacg acactctcgt
     1441 ctactccaag aatatcaaag atacagtctc agaagaccaa agggctattg agacttttca
     1501 acaaagggta atatcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat
     1561 caaaaggaca gtagaaaagg aaggtggcac ctacaaatgc catcattgcg ataaaggaaa
     1621 ggctatcgtt caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag
     1681 gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga
     1741 acatggtgga gcacgacact ctcgtctact ccaagaatat caaagataca gtctcagaag
     1801 accaaagggc tattgagact tttcaacaaa gggtaatatc gggaaacctc ctcggattcc
     1861 attgcccagc tatctgtcac ttcatcaaaa ggacagtaga aaaggaaggt ggcacctaca
     1921 aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga tgcctctgcc gacagtggtc
     1981 ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt
     2041 cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac gcacaatccc
     2101 actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg gagaggccgg
     2161 tctagagaat tccaagcaac gaactgcgag tgattcaaga aaaaagaaaa cctgagcttt
     2221 cgatctctac ggagtggttt cttgttcttt gaaaaagagg gggattacat atggctccta
     2281 agaagaagcg gaaggttggt attcacgggg tgcctgcggc tatggacaag aagtactcga
     2341 tcgggctggc catcggaaca aattctgtag gctgggctgt aataaccgat gagtacaagg
     2401 tgccctctaa aaaatttaag gtccttggca atacggatag acattccata aagaagaatc
     2461 ttatcggtgc gctgctcttt gacagcggcg agaccgcgga ggcgacccgg ttgaaacgca
     2521 ccgcgagacg ccgttacaca aggcgtaaga atagaatctg ttatctccag gagatattct
     2581 ctaatgaaat ggcgaaggta gacgattcct tctttcaccg tctggaggaa agttttctcg
     2641 ttgaggaaga taagaaacat gaaagacacc cgatcttcgg aaacattgtc gacgaggttg
     2701 cttatcatga aaagtaccct accatctacc atcttagaaa gaaacttgtt gacagcacgg
     2761 ataaggctga tctcaggctg atatacctgg ctctggcaca tatgattaag ttcagagggc
     2821 atttccttat cgaaggcgac ctgaatccag ataattcaga tgtagacaag ctcttcattc
     2881 aacttgtgca gacttataat cagctcttcg aagaaaatcc aataaacgcg tcgggtgtag
     2941 acgcaaaggc catactgtcc gctaggcttt ctaagtcacg tagacttgag aatctcattg
     3001 cccaactccc cggcgagaag aagaacggct tgtttggaaa tctgatagcg ctgtccctgg
     3061 gtcttacacc aaatttcaag agtaatttcg atttggcaga agatgctaag ttgcagctca
     3121 gtaaagacac ctacgatgac gatcttgata atttgttggc tcagattggc gatcagtatg
     3181 cagatctttt cttggccgct aagaatttgt ctgatgcaat tctgcttagc gacattttga
     3241 gggttaatac agaaatcacc aaggcaccct tgtcggcgtc aatgataaag aggtatgatg
     3301 agcaccacca agacctgacg ctcctcaagg ctcttgttcg gcagcaattg ccggagaagt
     3361 acaaagagat cttcttcgac cagtctaaga acggatatgc gggctacata gacggtggag
     3421 cgagtcagga ggaattctac aagttcataa agcccattct cgagaagatg gatggtacgg
     3481 aagaactgct tgtgaaactt aacagagaag atcttttgcg gaagcagaga actttcgaca
     3541 acggaagtat accacaccag atacatctcg gagagcttca tgctattctc agaagacaag
     3601 aggatttcta ccctttcttg aaggataaca gagaaaagat agagaagatc ctcacgttta
     3661 ggatccctta ctacgtaggt cctcttgctc gcggcaatag taggttcgcc tggatgaccc
     3721 gcaagtctga agaaactatc accccttgga atttcgaaga ggttgtagac aaaggtgctt
     3781 cagcacagag tttcattgag aggatgacca acttcgacaa gaacctcccc aacgaaaagg
     3841 tcctgcctaa gcacagcctc ctctacgaat actttactgt ctataatgag cttacaaaag
     3901 ttaagtacgt gacagaggga atgcggaagc ccgcattcct ttccggagaa caaaagaagg
     3961 cgatcgtgga tcttctcttc aagacgaacc gcaaggtgac ggttaaacag ttgaaggaag
     4021 attacttcaa gaagatagaa tgttttgata gcgtggaaat cagcggcgtc gaagataggt
     4081 tcaacgcttc cctgggaacg taccacgatc tcctcaagat tatcaaagat aaggactttc
     4141 ttgataacga agagaatgag gacatcttgg aagacattgt tctgacgctc accctgttcg
     4201 aagatcgcga gatgattgag gaacgcttga agacctacgc acacctgttc gatgacaagg
     4261 ttatgaagca acttaaacgg cgccggtata cgggctgggg acggctttcg cggaagctga
     4321 taaatggaat ccgtgacaag cagtctggca agacaatact cgacttcttg aagtcggatg
     4381 gttttgccaa tagaaatttt atgcaactca ttcatgatga ctcgcttact tttaaggagg
     4441 acatccagaa ggcccaggta tcaggacagg gtgactcttt gcacgaacac atcgcgaacc
     4501 tggcgggctc ccccgcgatt aagaagggaa ttttgcagac tgtcaaggtg gtcgatgaac
     4561 tcgtgaaggt tatgggacgt cataagccgg aaaatattgt gattgagatg gctcgcgaga
     4621 atcaaacaac acagaagggc caaaagaaca gtagagaacg catgaagcgc atcgaagagg
     4681 gcatcaaaga gctgggcagt cagatcctta aagaacatcc agtcgagaat acacagcttc
     4741 agaacgaaaa gctgtacctt tattaccttc aaaatgggcg tgatatgtat gtggatcagg
     4801 aactcgatat caataggctg agtgactatg atgtcgacca tatcgtcccg caaagtttcc
     4861 tcaaggacga cagtatagac aacaaagttc tcacacggtc agataagaat cgcggcaaga
     4921 gcgataatgt accgtcggag gaggtagtca agaagatgaa gaattactgg cgccagttgc
     4981 tcaacgccaa gctcatcact cagaggaaat ttgacaacct tacgaaagcc gagcggggcg
     5041 gactctctga actggacaag gccggtttca taaagcgcca gctcgttgag acacgtcaaa
     5101 ttactaagca cgtcgctcaa atattggatt cccgcatgaa tactaagtac gatgagaatg
     5161 ataagctcat acgtgaagtt aaggtcatta ctctcaagtc caagcttgta tcggacttcc
     5221 gtaaggactt ccaattctac aaggtccggg aaatcaataa ttatcaccat gcccatgacg
     5281 cttatctgaa cgcggtcgtg ggcacggcac tcattaagaa atacccaaaa cttgagtcag
     5341 aatttgttta cggggactat aaagtttatg acgtgcggaa gatgatagcg aagtcggaac
     5401 aagagatagg aaaggcgact gcaaagtact ttttttactc caacataatg aatttcttta
     5461 agaccgaaat aacccttgca aacggtgaaa tcagaaagcg gcctctgatt gaaacaaatg
     5521 gcgagacggg cgagatcgtc tgggacaagg ggagggactt cgcaacggtt cgcaaggtcc
     5581 ttagcatgcc gcaagtaaat atagttaaga agacggaagt tcagaccggc ggctttagta
     5641 aagaaagcat acttcctaaa aggaattccg acaaactgat agcgcgcaag aaggactggg
     5701 atccaaagaa gtatggagga tttgactccc caaccgttgc ttatagcgtg ttggtagtag
     5761 ccaaggtgga aaagggtaag tctaagaaat tgaagtcggt gaaggagttg ttggggataa
     5821 ctataatgga gcggagttcg ttcgagaaga acccaattga ctttctcgaa gccaaaggct
     5881 acaaggaggt caagaaggac ctgattatta agttgccaaa gtactcgctc ttcgaactcg
     5941 agaacgggag aaagcgtatg ctggcgtcgg cgggcgagct gcagaaagga aacgagctgg
     6001 ctttgccatc gaaatacgta aatttcctgt acctcgcctc acattatgag aagcttaaag
     6061 ggtctccaga agacaatgaa cagaagcagc tgtttgttga acagcacaag cactacttgg
     6121 acgagattat agaacaaatc tccgagttct ctaaacgggt tatccttgca gacgccaatt
     6181 tggataaggt cctctcggct tataataagc atagagataa gccaatccgg gagcaggctg
     6241 aaaatatcat acacctcttt acgttgacta atttgggtgc gccagcggca ttcaagtact
     6301 tcgatacaac aatcgatcgt aagcgctaca caagcactaa ggaagtcctg gacgcgacgc
     6361 tgatacacca gtccattact ggactgtatg aaaccagaat agatcttagc cagctcggcg
     6421 gtgatggagg agggcctagg gctgatccta agaagaagag gaaggttgga ggagggccag
     6481 gagcagagta tgttagagcg ttgtttgatt ttaacggtaa cgatgaagag gatttaccct
     6541 ttaagaaagg cgacattctc aggattaggg ataaacctga agagcaatgg tggaatgctg
     6601 aggatagtga aggcaaacga ggaatgattt tagtgccgta tgtggagaaa tattcgggtg
     6661 actacaaaga tcatgatggt gattacaaag accatgacat cgactacaag gatgatgatg
     6721 ataagtcagg gatgacagat gctgaatatg tcagaatcca cgaaaagttg gacatttaca
     6781 cgtttaagaa gcagttcttc aacaacaaga aatctgtttc gcataggtgc tatgtgcttt
     6841 tcgaactaaa acgtcgtgga gaaagacggg cttgcttttg gggttacgcg gttaacaaac
     6901 cacaatcagg tactgaacga ggaatacacg ctgaaatctt ttctatccga aaggttgagg
     6961 aatatctacg tgacaatcct ggacagttca ctatcaattg gtattctagc tggtcaccat
     7021 gtgcagattg tgctgagaag attctcgaat ggtacaatca agagcttaga ggcaatggac
     7081 atacattgaa aatatgggca tgcaagctct actacgaaaa gaatgccaga aaccaaattg
     7141 ggctttggaa cttgagggat aatggagttg ggcttaatgt catggtttct gagcactatc
     7201 aatgttgtcg gaagatcttc atacaaagtt cccataacca gttgaatgag aacagatggt
     7261 tagagaaaac ccttaaaaga gccgagaaga gaagatccga actgagcatt atgatacagg
     7321 tcaaaattct gcataccact aagagtccag ctgtaggtcc taagaagaaa cgtaaagtag
     7381 ggccctgatc caggcctccc agctttcgtc cgtatcatcg gtttcgacaa cgttcgtcaa
     7441 gttcaatgca tcagtttcat tgcccacaca ccagaatcct actaagtttg agtattatgg
     7501 cattggaaaa gctgttttct tctatcattt gttctgcttg taatttactg tgttctttca
     7561 gtttttgttt tcggacatca aaatgcaaat ggatggataa gagttaataa atgatatggt
     7621 ccttttgttc attctcaaat tattattatc tgttgttttt actttaatgg gttgaattta
     7681 agtaagaaag gaactaacag tgtgatatta aggtgcaatg ttagacatat aaaacagtct
     7741 ttcacctctc tttggttatg tcttgaattg gtttgtttct tcacttatct gtgtaatcaa
     7801 gtttactatg agtctatgat caagtaatta tgcaatcaag ttaagtacag tataggcttg
     7861 agctccctag gcccctaggt aattcttcgg acccaagaat gctaagccaa gaggagctgt
     7921 tatcgccgtc ctcctgcttg tttctctctt tttgttgctg tttcttcatt agcgtggaca
     7981 aagttttcaa ccggcctatc tgttatcatt ttcttctatt caaagactgt aatacctatt
     8041 gctacctgtg gttctcactt gtgattttgg acacatatgt tcggtttatt caaatttaat
     8101 cagatgcctg atgagggtac cagaaaaaat acgtgttctg gttgtttttg agttgcgatt
     8161 attctatgaa atgaataaca tcgaagttat catcccagta ttttcgcatg aatgttcttt
     8221 tcttctgtct tgtgcatcag tgatctagtg catgggagtt tgtattgtga tgttcgacat
     8281 cacgtaactt ccactttgcc tttgctgttc gatattttaa tgacatgtca cacacacttc
     8341 tgatactttt ctttcttggc tattgtgcca gcatgatgca agatgcatca cagcatcaga
     8401 tatattctca tcgtcaggct ttagcagcac acgagcacgc tttgccgctt aaaagttgta
     8461 cggcgcagct tagacatccc ctgtagaagt gataatcttt tcacttttcc ttaaacaaat
     8521 tgagagggga aatggaacca tgtggatcag agaagctttt gtttctttac acaagaatat
     8581 ttggtacagt gggggtccta tgttcgtggg ttcgtggctt ggctgcctgt cttcaaccaa
     8641 gtgttttcag ttcaacatgt tagcgtgtag aaagagcaca attctgttta tctccaaggt
     8701 aaaatgtggc attctgttaa agaacatgat cctgccaatt ttttaagttt caatggaaga
     8761 ggaatgtaaa gctttctatg gtttgtgtac acaacacagt ggaagaggag tgcaagcttt
     8821 ctatggtttg tgtgcgcgtt gtgtgtcagc acttcaattt tgttagaaaa tgaaagaaaa
     8881 aaaaggatga tcatgcttat agtaaatcac tctttttcct cgccttctgt acgttttgac
     8941 ttgacaagat tttaaaatct gtacatgacc tttgttttaa aattacttta tgtatttcca
     9001 tctttcaagt tatgcagatg tcatcacaaa ttgttacacc aatcaccagg ctggctgttt
     9061 atatattatc agaccaggct atatagagta tactatacta actgttcata ttatctggaa
     9121 atcttgcttg ctacttgagc ggtaaaaggg tatagatatg agggtcccca gattagcctt
     9181 ttcaatttca gaaagaatgc taacccacag atggttagag aggcttacgc agcaggtctc
     9241 atcaagacga tctacccgag caataatctc caggaaatca aataccttcc caagaaggtt
     9301 aaagatgcag tcaaaagatt caggactaac tgcatcaaga acacagagaa agatatattt
     9361 ctcaagatca gaagtactat tccagtatgg acgattcaag gcttgcttca caaaccaagg
     9421 caagtaatag agattggagt ctctaaaaag gtagttccca ctgaatcaaa ggccatggag
     9481 tcaaagattc aaatagagga cctaacagaa ctcgccgtaa agactggcga acagttcata
     9541 cagagtctct tacgactcaa tgacaagaag aaaatcttcg tcaacatggt ggagcacgac
     9601 acacttgtct actccaaaaa tatcaaagat acagtctcag aagaccaaag ggcaattgag
     9661 acttttcaac aaagggtaat atccggaaac ctcctcggat tccattgccc agctatctgt
     9721 cactttattg tgaagatagt ggaaaaggaa ggtggctcct acaaatgcca tcattgcgat
     9781 aaaggaaagg ccatcgttga agatgcctct gccgacagtg gtcccaaaga tggaccccca
     9841 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat
     9901 tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc ttcgcaagac
     9961 ccttcctcta tataaggaag ttcatttcat ttggagagaa cacgggggac tctagaatga
    10021 aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg
    10081 tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag
    10141 gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt
    10201 atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg
    10261 agtttagcga gagcctgacc tattgcatct cccgccgttc acagggtgtc acgttgcaag
    10321 acctgcctga aaccgaactg cccgctgttc tacaaccggt cgcggaggct atggatgcga
    10381 tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg
    10441 gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact
    10501 ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga
    10561 tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca
    10621 acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt
    10681 tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta
    10741 tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccacgac
    10801 tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca
    10861 atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg
    10921 ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg
    10981 tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccgagg gcaaagaaat
    11041 aggagctctg ggctaatcta aaacgattta tctgtggctt caagtgtatc gatcacttat
    11101 gtgaggtgta attactggtg tttttggtgt gctctggttc ctttcaagtg tgttgttgcc
    11161 gctcgaacta ctccgctatg taaaacggta aaacctgttg tctcattatg aaagtgaact
    11221 atattatgtt ctactactac tctacttagt caattttctt caccttgatt agtgtaaata
    11281 tgaatcttat attcttatgt cttaagaaat tagcacatgt gaagcctcca agtgcatatt
    11341 ttctcgatcg cgagacgcac aatgcgtgag aaattcagct ggttatactc aaatatatta
    11401 atatatctag cagcagctca tggagattca ggaaacttgg catccctaat ccctaccatt
    11461 tccattcttc cgagattgac agttcaatac aagtacagta atctcctggt aagtttctta
    11521 ttaacttgac atgtagtagt aataatttgt acgtagcata gatacataga cacaaaaatg
    11581 tcctccccat tgagctagcc gattggagcc gaacaccgca ggaatgaatt tacataatct
    11641 gcaaagaata aatggaatgt gcctccacag gaaaaccagc ggcagtgtgg cgttttcaag
    11701 agcagccgta agtcgaagcc tattctgaat cgtagaaatc actggggcat ggtgtaatta
    11761 catccgactc caacatctgt accaccctgt gcattgtagg ccgctcctct ggcaaagaac
    11821 ttacacattg tttagcaaga gaaagtagag catccaaggt ctcaatctgc actccctcac
    11881 aatatggatc gacaatttcc ctctcccgat tctcaccgac caggaaattc aactgccaca
    11941 gagcaagtag attatttcaa gaatacatta aatcaattga aggcatacgt aattcatatc
    12001 agaaaactgt ggatatgaaa tggaatgaca taaaggtcat acatacccat ccaacaatgt
    12061 tcaatccctt ttcaataaat gatgcatcag taggtcgttt tccgcttagt atttcaagta
    12121 gcaaaactcc aaaactgtag acgtcagtct tttcggtggc tctgccactt tgcatatact
    12181 cctgcaggtc gaccatagtg actggatatg ttgtgtttta cagtattatg tagtctgttt
    12241 tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt ctcgttcagc
    12301 tttcttgtac aaagtggtga tatcccgcgg aaatacgtag aattcgggaa gcttggcact
    12361 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
    12421 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
    12481 ttcccaacag ttgcgcagcc tgaatggcga atgctagagc aattcggcgt taattcagta
    12541 cattaaaaac gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt ttacaccaca
    12601 atatatcctg ccaccagcca gccaacagct ccccgaccgg cagctcggca caaaatcacc
    12661 actcgataca ggcagcccat cagtccggga cggcgtcagc gggagagccg ttgtaaggcg
    12721 gcagactttg ctcatgttac cgatgctatt cggaagaacg gcaactaagc tgccgggttt
    12781 gaaacacgga tgatctcgcg gagggtagca tgttgattgt aacgatgaca gagcgttgct
    12841 gcctgtgatc aattcgggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt
    12901 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg
    12961 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta
    13021 tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga
    13081 gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac atcatggggg
    13141 aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc
    13201 atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga
    13261 agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc
    13321 ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc
    13381 tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag
    13441 ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg
    13501 agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg
    13561 ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat
    13621 ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg
    13681 agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg
    13741 cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg
    13801 tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg
    13861 cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca
    13921 aataatgtct agctagaaat tcgttcaagc cgacgccgct tcgcggcgcg gcttaactca
    13981 agcgttagat gcactaagca cataattgct cacagccaaa ctatcaggtc aagtctgctt
    14041 ttattatttt taagcgtgca taataagccc tacacaaatt gggagatata tcatgcatga
    14101 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
    14161 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
    14221 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
    14281 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
    14341 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
    14401 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
    14461 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
    14521 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
    14581 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
    14641 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
    14701 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
    14761 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
    14821 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
    14881 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
    14941 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
    15001 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
    15061 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
    15121 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
    15181 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg
    15241 ggcgccggcg gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc
    15301 gtaggccagc catttttgag cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc
    15361 gtagggagcg cagcgaccga agggtaggcg ctttttgcag ctcttcggct gtgcgctggc
    15421 cagacagtta tgcacaggcc aggcgggttt taagagtttt aataagtttt aaagagtttt
    15481 aggcggaaaa atcgcctttt ttctctttta tatcagtcac ttacatgtgt gaccggttcc
    15541 caatgtacgg ctttgggttc ccaatgtacg ggttccggtt cccaatgtac ggctttgggt
    15601 tcccaatgta cgtgctatcc acaggaaaga gaccttttcg acctttttcc cctgctaggg
    15661 caatttgccc tagcatctgc tccgtacatt aggaaccggc ggatgcttcg ccctcgatca
    15721 ggttgcggta gcgcatgact aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt
    15781 actccggcag gtcatttgac ccgatcagct tgcgcacggt gaaacagaac ttcttgaact
    15841 ctccggcgct gccactgcgt tcgtagatcg tcttgaacaa ccatctggct tctgccttgc
    15901 ctgcggcgcg gcgtgccagg cggtagagaa aacggccgat gccgggatcg atcaaaaagt
    15961 aatcggggtg aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg cggtacatcc
    16021 aatcagctag ctcgatctcg atgtactccg gccgcccggt ttcgctcttt acgatcttgt
    16081 agcggctaat caaggcttca ccctcggata ccgtcaccag gcggccgttc ttggccttct
    16141 tcgtacgctg catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt
    16201 ctttctgctt tccgccatcg gctcgccggc agaacttgag tacgtccgca acgtgtggac
    16261 ggaacacgcg gccgggcttg tctcccttcc cttcccggta tcggttcatg gattcggtta
    16321 gatgggaaac cgccatcagt accaggtcgt aatcccacac actggccatg ccggccggcc
    16381 ctgcggaaac ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc
    16441 ggtcacgctt cgacagacgg aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc
    16501 ccacgtcata gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc
    16561 taatcgacgg cgcaccggct gccggcggtt gccgggattc tttgcggatt cgatcagcgg
    16621 ccgcttgcca cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct
    16681 gcgcggcctt caacttctcc accaggtcat cacccagcgc cgcgccgatt tgtaccgggc
    16741 cggatggttt gcgaccgctc acgccgattc ctcgggcttg ggggttccag tgccattgca
    16801 gggccggcag acaacccagc cgcttacgcc tggccaaccg cccgttcctc cacacatggg
    16861 gcattccacg gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc ctttagccgc
    16921 taaaattcat ctactcattt attcatttgc tcatttactc tggtagctgc gcgatgtatt
    16981 cagatagcag ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt
    17041 gatcctccgc cggcaactga aagttgaccc gcttcatggc tggcgtgtct gccaggctgg
    17101 ccaacgttgc agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc
    17161 ttttgctcat tttctcttta cctcattaac tcaaatgagt tttgatttaa tttcagcggc
    17221 cagcgcctgg acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc
    17281 ggcggcggca gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa gaatgggcag
    17341 ctcgtacccg gccagcgcct cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg
    17401 cgacacgaca aaggccgctt gtagccttcc atccgtgacc tcaatgcgct gcttaaccag
    17461 ctccaccagg tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg gaatcagcac
    17521 gaagtcggct gccttgatcg cggacacagc caagtccgcc gcctggggcg ctccgtcgat
    17581 cactacgaag tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat
    17641 gccgacaacg gttagcggtt gatcttcccg cacggccgcc caatcgcggg cactgccctg
    17701 gggatcggaa tcgactaaca gaacatcggc cccggcgagt tgcagggcgc gggctagatg
    17761 ggttgcgatg gtcgtcttgc ctgacccgcc tttctggtta agtacagcga taaccttcat
    17821 gcgttcccct tgcgtatttg tttatttact catcgcatca tatacgcagc gaccgcatga
    17881 cgcaagctgt tttactcaaa tacacatcac ctttttagac ggcggcgctc ggtttcttca
    17941 gcggccaagc tggccggcca ggccgccagc ttggcatcag acaaaccggc caggatttca
    18001 tgcagccgca cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc
    18061 tccgcctcga tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc
    18121 atgcttgttc ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg
    18181 cgtcctcacg gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct
    18241 caagtgcgcg gtacagggtc gagcgatgca cgccaagcag tgcagccgcc tctttcacgg
    18301 tgcggccttc ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag
    18361 ggcgggggcc aaacttcacg cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt
    18421 cgatgattag ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac accatgcggc
    18481 cggccggcgt ggtggtgtcg gcccacggct ctgccaggct acgcaggccc gcgccggcct
    18541 cctggatgcg ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc
    18601 tggtcactgt cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc
    18661 ggtcgcgcct ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca
    18721 gttcggcccg ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca
    18781 ggccagcggc ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct
    18841 actttatgcg actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct
    18901 gtcgcgtaac ttaggacttg tgcgacatgt cgttttcaga agacggctgc actgaacgtc
    18961 agaagccgac tgcactatag cagcggaggg gttggatcaa agtacttt
//

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