pAAVLINKv2-SCP1-Cre-UDeg3a-NIPBL-C-FLAG

LOCUS       pAAVLINKv2-SCP1-Cre-UDeg3a-NIPBL-C-FLAG               7044 bp DNA     circular SYN 10-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7044)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 20, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7044
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     misc_feature    145..225
                     /label=SCP1
     CDS             237..1265
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             1281..1370
                     /codon_start=1
                     /label=UDeg3a
                     /translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
     misc_feature    1295..1300
                     /label=SDS
     misc_feature    1298..1303
                     /label=SDS
     intron          1374..1414
                     /label=Splicing Donor
     protein_bind    1421..1454
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1455..1546
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1547..3748
                     /codon_start=1
                     /gene="NIPBL"
                     /gene_synonym="CDLS; CDLS1; IDN3; IDN3-B; Scc2"
                     /product="nipped-B-like protein isoform A"
                     /label=NIPBL
                     /note="isoform A is encoded by transcript variant A;sister 
                     chromatid cohesion 2 homolog; nipped-B-like protein; SCC2 
                     homolog; delangin; Nipped-B homolog"
                     /db_xref="GI:47578105"
                     /db_xref="CCDS:CCDS3920.1"
                     /db_xref="GeneID:25836"
                     /db_xref="HGNC:HGNC:28862"
                     /db_xref="MIM:608667"
                     /protein_id="NP_597677.2"
                     /translation="EDLMKLIIKYGMTVVQHCVSCLGAVVNKVTQNFKFVWACFNRYYG
                     AISKLKSQHQEDPNNTSLLTNKPALLRSLFTVGALCRHFDFDLEDFKGNSKVNIKDKVL
                     ELLMYFTKHSDEEVQTKAIIGLGFAFIQHPSLMFEQEVKNLYNNILSDKNSSVNLKIQV
                     LKNLQTYLQEEDTRMQQADRDWKKVAKQEDLKEMGDVSSGMSSSIMQLYLKQVLEAFFH
                     TQSSVRHFALNVIALTLNQGLIHPVQCVPYLIAMGTDPEPAMRNKADQQLVEIDKKYAG
                     FIHMKAVAGMKMSYQVQQAINTCLKDPVRGFRQDESSSALCSHLYSMIRGNRQHRRAFL
                     ISLLNLFDDTAKTDVTMLLYIADNLACFPYQTQEEPLFIMHHIDITLSVSGSNLLQSFK
                     ESMVKDKRKERKSSPSKENESSDSEEEVSRPRKSRKRVDSDSDSDSEDDINSVMKCLPE
                     NSAPLIEFANVSQGILLLLMLKQHLKNLCGFSDSKIQKYSPSESAKVYDKAINRKTGVH
                     FHPKQTLDFLRSDMANSKITEEVKRSIVKQYLDFKLLMEHLDPDEEEEEGEVSASTNAR
                     NKAITSLLGGGSPKNNTAAETEDDESDGEDRGGGTSGSLRRSKRNSDSTELAAQMNESV
                     DVMDVIAICCPKYKDRPQIARVVQKTSSGFSVQWMAGSYSGSWTEAKRRDGRKLVPWVD
                     TIKESDIIYKKIALTSANKLTNKVVQTLRSLYAAKDGTSS"
     misc_feature    3301..3306
                     /label=SDS
     misc_feature    3367..3372
                     /label=SDS
     misc_feature    3370..3375
                     /label=SDS
     CDS             3752..3817
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3833..4264
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    3977..3982
                     /label=SDS
     repeat_region   4307..4447
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4307..4436
                     /label=AAV2 ITR
     repeat_region   4308..4448
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4522..4977
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5259..5363
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5364..6224
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6395..6983
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
      181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
      241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
      301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
      361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
      421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
      481 gtctggcagt aaaaactatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
      541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
      601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
      661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
      721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
      781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
      841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
      901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
      961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
     1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
     1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
     1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
     1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
     1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
     1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
     1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
     1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
     1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggaag atctaatgaa
     1561 gctcatcatc aaatatggca tgactgtagt gcaacattgt gtgagctgtc ttggagctgt
     1621 tgtaaataaa gtgacacaaa attttaaatt tgtgtgggct tgtttcaata gatactatgg
     1681 tgccatttca aaattaaaaa gtcaacacca agaggaccca aataacactt cacttctaac
     1741 aaacaaacca gcacttctta gatccctttt caccgttgga gcactatgtc ggcattttga
     1801 ttttgatctg gaagatttta aaggcaacag caaggttaac ataaaagata aagtacttga
     1861 actattgatg tattttacaa aacactcaga tgaagaagta caaacaaaag ctatcattgg
     1921 tctaggattt gcctttattc agcatccaag tctaatgttc gagcaagaag tgaagaatct
     1981 atataataat attttatctg ataagaactc ctcagtcaat ttaaaaatac aagtgttaaa
     2041 aaacctccag acctacctac aagaagaaga tacacgtatg cagcaggcag atagagactg
     2101 gaagaaagtt gcaaaacagg aagacttaaa agaaatgggt gatgtttcct cagggatgag
     2161 tagttccatc atgcagcttt atctcaaaca ggtgcttgag gcattttttc acacccagtc
     2221 aagtgtacgc cactttgccc taaatgtcat tgcattgact ctaaatcaag gtcttattca
     2281 tccagttcag tgtgtgccat atttaattgc tatgggcaca gacccagaac ctgctatgcg
     2341 gaacaaggct gatcagcaac ttgtggaaat agacaaaaaa tatgctggat tcattcatat
     2401 gaaagcagtg gctggtatga agatgtctta ccaggtacaa caggcaatca acacatgcct
     2461 aaaagatcct gtaaggggtt tcagacaaga cgagtcctct agcgctttgt gttcacacct
     2521 ttactccatg atccgtggaa accgccaaca cagacgagcc tttcttattt ctttactcaa
     2581 cctctttgat gacacagcaa aaacagacgt gactatgctc ttgtatatag cagacaatct
     2641 agcctgtttt ccataccaga cacaggaaga gccgttgttt ataatgcatc atatagacat
     2701 tacactctca gtttctggta gtaacctact gcagtcattc aaggagtcta tggtaaagga
     2761 caaaaggaaa gagagaaaat catcacctag taaggaaaat gagtcaagcg acagtgaaga
     2821 agaagtttcc aggcctcgga agtcacggaa acgtgtagat tcagattcag attcagattc
     2881 agaagacgat ataaattcag tgatgaaatg tttgccagaa aattcagctc ctttaatcga
     2941 atttgcaaat gtgtcccagg gtattttatt acttctcatg ttaaaacaac atttgaagaa
     3001 tctttgtgga ttttctgata gtaaaattca gaagtactct ccatctgaat ctgcaaaagt
     3061 atatgataaa gcgataaacc gaaaaacagg agttcatttt catccaaaac aaacactgga
     3121 cttcctgcgg agtgacatgg ctaattccaa aatcacagaa gaggtgaaaa ggagtatagt
     3181 aaaacagtat ctagatttca aacttctcat ggaacatctg gaccctgatg aagaagaaga
     3241 agaaggggag gtttcagcta gcacaaatgc tcggaacaaa gcaattacct cactgcttgg
     3301 aggaggcagc cctaaaaata atacagcagc agagacagaa gatgatgaaa gtgatgggga
     3361 ggatagagga ggaggcactt cagggtcatt gagaaggtca aaacgaaatt cagactctac
     3421 ggagttggca gcacagatga atgaaagtgt tgacgtcatg gatgtcatcg ctatttgctg
     3481 tccaaagtac aaagatcgac cacaaattgc aagagtagtg cagaaaacca gcagtggctt
     3541 cagtgttcag tggatggcag gctcctacag tggctcctgg actgaggcta agcgccgtga
     3601 tggccgcaaa ctggtgcctt gggtagacac tattaaagag tcagacatta tttacaaaaa
     3661 aattgctcta acgagtgcta ataagctgac taataaagtt gttcagactt tacgatccct
     3721 gtatgccgcc aaggatggga cttccagcaa ggactacaaa gaccatgacg gtgattataa
     3781 agatcatgac atcgactaca aggatgacga tgacaagtaa gaattctgat gactcgagat
     3841 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     3901 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     3961 atggctttca ttttctcctc cttgtataaa tcctggttag ttcttgccac ggcggaactc
     4021 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     4081 gtggtgttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat ctagctttat
     4141 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     4201 taacaacaac aattgcattc attttatgtt tcaggttcag ggggagatgt gggaggtttt
     4261 ttaaagatct ggagaccggt gtggcacgtg cggaccgagc ggccgcagga acccctagtg
     4321 atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag
     4381 gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc
     4441 ctgcaggggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     4501 atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     4561 tggttacgcg cagcgtgacc gctacacttg ccagcgcctt agcgcccgct cctttcgctt
     4621 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     4681 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg
     4741 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     4801 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aactctatct
     4861 cgggctattc ttttgattta taagggattt tgccgatttc ggtctattgg ttaaaaaatg
     4921 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat
     4981 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
     5041 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     5101 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     5161 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     5221 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     5281 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     5341 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     5401 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     5461 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     5521 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     5581 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     5641 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     5701 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     5761 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     5821 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     5881 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     5941 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     6001 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     6061 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     6121 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     6181 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     6241 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     6301 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     6361 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     6421 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     6481 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     6541 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     6601 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     6661 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     6721 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     6781 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     6841 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     6901 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     6961 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     7021 tttgctggcc ttttgctcac atgt
//