| 出品公司: | NIH | |
|---|---|---|
| 载体名称: | pNL4-3、pNL4.3 | |
| 载体又名： | HIV-1 NL4-3 Infectious Molecular Clone (pNL4-3)、 HIV-1 vector pNL4-3、pNL4-3载体、pNL4.3载体 | |
| 质粒类型: | 哺乳动物细胞载体；逆病毒包装载体 | |
| 高拷贝/低拷贝: | 高拷贝 | |
| 克隆方法: | 限制性内切酶，多克隆位点 | |
| 启动子: | CMV | |
| 载体大小: | 14825 bp | |
| 5' 测序引物及序列: | -- | |
| 3' 测序引物及序列: | -- | |
| 载体序列： | Genbank: AF324493 | |
| 存储人: | Dr. Malcolm Mar | |
| 载体抗性: | 氨苄青霉素 | |
| 筛选标记: | 嘌呤霉素（Puromycin） | |
| 克隆菌株: | DH5α 等 | |
| 宿主细胞（系）: | 常规细胞系（293、CV-1、CHO等） | |
| 备注: |
一个包含有全长基因，复制能力强，能够感染HIV-1亚型B NL4-3蛋白的分子克隆载体。
这个载体产生的病毒利用CXCR4作为共同受体。
该载体为大小为14825 bp，包括插入片段。
这个嵌合载体的来源是HIV-1ny5分离物5’ 从基因组DNA中直接克隆的LAV分离物3’。前病毒NY5（5’SmaI和 3’EcoRI）前病毒3’片段（5’EcoRI 和3’NruI）被钝端克隆到PvuII位点的pUC18中 删除多段连接片段后。
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| 产品目录号: | #114 | |
| 稳定性: | 稳表达 | |
| 组成型/诱导型: | 组成型 | |
| 病毒/非病毒: | 非病毒 | |
| 生物危害性: | 无 | |
| 参考文献: |
Adachi, A., Gendelman, H. E., Koenig, S., Folks, T., Willey, R., Rabson, A., & Martin, M. A. (1986). Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone. J Virol, 59(2), 284-291. PUBMED
|
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| 载体说明书: | pNL4-3载体说明书 | |
| 相关载体: |
pNL4-3-Luc-R-E- NL4-3-ΔEnv-EGFP |
买家导航
pNL4.3载体HIV-1载体基本信息
pNL4.3载体HIV-1载体图谱质粒图谱和多克隆位点信息
pNL4.3载体HIV-1载体简介
pNL4.3载体HIV-1载体序列
LOCUS pNL4-3 14825 bp DNA circular SYN 25-JUL-2016
DEFINITION HIV-1 vector pNL4-3, complete sequence.
ACCESSION AF324493
VERSION AF324493.2
KEYWORDS .
SOURCE HIV-1 vector pNL4-3
ORGANISM HIV-1 vector pNL4-3
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 9709)
AUTHORS Adachi,A., Gendelman,H.E., Koenig,S., Folks,T., Willey,R.,
Rabson,A. and Martin,M.A.
TITLE Production of acquired immunodeficiency syndrome-associated
retrovirus in human and nonhuman cells transfected with an
infectious molecular clone
JOURNAL J. Virol. 59 (2), 284-291 (1986)
PUBMED 3016298
REFERENCE 2 (bases 1 to 14825)
AUTHORS Bosche,W.J., Poon,D.T.K., Ott,D.E., Hu,W.-S. and Gorelick,R.J.
TITLE Complete Plasmid Sequence of pNL4-3
JOURNAL Unpublished
REFERENCE 3 (bases 1 to 14825)
AUTHORS Bosche,W.J., Poon,D.T.K., Ott,D.E., Hu,W.-S. and Gorelick,R.J.
TITLE Direct Submission
JOURNAL Submitted (28-NOV-2000) AIDS Vaccine Program, SAIC Frederick and
Drug Resistance Program, National Cancer Institute at Frederick, PO
Box B, Frederick, Maryland 21702-1202, USA
REFERENCE 4 (bases 1 to 14825)
AUTHORS Strebel,K.J. and Martin,M.A.
TITLE Direct Submission
JOURNAL Submitted (24-MAY-2010) NIAID, Viral Biochemistry, National
Institutes of Health, Bethesda, MD, USA
REMARK Sequence update by submitter
COMMENT On May 24, 2010 this sequence version replaced AF324493.1.
FEATURES Location/Qualifiers
source 1..14825
/organism="HIV-1 vector pNL4-3"
/mol_type="other DNA"
/db_xref="taxon:151458"
/plasmid="pNL4-3"
/focus
source 1..9709
/organism="Human immunodeficiency virus 1"
/proviral
/mol_type="other DNA"
/strain="NY5/BRU (LAV-1)"
/db_xref="taxon:11676"
/note="from sequence deposited in GenBank Accession Number
M19921"
repeat_region 1..634
/note="5' long terminal repeat"
/rpt_type=long_terminal_repeat
repeat_region 454..550
/note="R; 5' copy"
CDS 790..2292
/codon_start=1
/transl_table=11
/product="gag polyprotein"
/protein_id="AAK08483.1"
/translation="MGARASVLSGGELDKWEKIRLRPGGKKQYKLKHIVWASRELERF
AVNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTIAVLYCVHQRIDVKDTKEALD
KIEEEQNKSKKKAQQAAADTGNNSQVSQNYPIVQNLQGQMVHQAISPRTLNAWVKVVE
EKAFSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRLHPV
HAGPIAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRM
YSPTSILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTIL
KALGPGATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKC
FNCGKEGHIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQ
SRPEPTAPPEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
CDS <2085..5096
/codon_start=1
/transl_table=11
/product="pol polyprotein"
/protein_id="AAK08484.2"
/translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEA
GADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKM
IGGIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPI
ETVPVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAI
KKKDSTKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLD
KDFRKYTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIV
IYQYMDDLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPD
KWTVQPIVLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPL
TEEAELELAENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGK
YARMKGAHTNDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWI
PEWEFVNTPPLVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPL
TDTTNQKTELQAIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIK
KEKVYLAWVPAHKGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASD
FNLPPVVAKEIVASCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGY
IEAEVIPAETGQETAYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGI
PYNPQSQGVIESMNKELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGER
IVDIIATDIQTKELQKQITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDI
KVVPRRKAKIIRDYGKQMAGDDCVASRQDED"
misc_difference 2421
/note="compared to sequence deposited in GenBank Accession
Number M19921; results in arginine to glycine
substitution"
/replace="g"
CDS 5041..5619
/codon_start=1
/transl_table=11
/product="vif protein"
/protein_id="AAK08482.1"
/translation="MENRWQVMIVWQVDRMRINTWKRLVKHHMYISRKAKDWFYRHHY
ESTNPKISSEVHIPLGDAKLVITTYWGLHTGERDWHLGQGVSIEWRKKRYSTQVDPDL
ADQLIHLHYFDCFSESAIRNTILGRIVSPRCEYQAGHNKVGSLQYLALAALIKPKQIK
PPLPSVRKLTEDRWNKPQKTKGHRGSHTMNGH"
CDS 5559..5849
/codon_start=1
/transl_table=11
/product="vpr protein"
/protein_id="AAK08485.1"
/translation="MEQAPEDQGPQREPYNEWTLELLEELKSEAVRHFPRIWLHNLGQ
HIYETYGDTWAGVEAIIRILQQLLFIHFRIGCRHSRIGVTRQRRARNGASRS"
misc_feature 5743..5748
/note="EcoRI site of recombination"
CDS join(5830..6044,8369..8414)
/codon_start=1
/transl_table=11
/product="tat protein"
/protein_id="AAK08486.1"
/translation="MEPVDPRLEPWKHPGSQPKTACTNCYCKKCCFHCQVCFMTKALG
ISYGRKKRRQRRRAHQNSQTHQASLSKQPTSQSRGDPTGPKE"
CDS join(5969..6044,8369..8643)
/codon_start=1
/transl_table=11
/product="rev protein"
/protein_id="AAK08487.1"
/translation="MAGRSGDSDEELIRTVRLIKLLYQSNPPPNPEGTRQARRNRRRR
WRERQRQIHSISERILSTYLGRSAEPVPLQLPPLERLTLDCNEDCGTSGTQGVGSPQI
LVESPTVLESGTKE"
CDS 6061..6306
/codon_start=1
/transl_table=11
/product="vpu protein"
/protein_id="AAK08488.1"
/translation="MQPIIVAIVALVVAIIIAIVVWSIVIIEYRKILRQRKIDRLIDR
LIERAEDSGNESEGEVSALVEMGVEMGHHAPWDIDDL"
CDS 6221..8785
/codon_start=1
/transl_table=11
/product="envelope polyprotein"
/protein_id="AAK08489.2"
/translation="MRVKEKYQHLWRWGWKWGTMLLGILMICSATEKLWVTVYYGVPV
WKEATTTLFCASDAKAYDTEVHNVWATHACVPTDPNPQEVVLVNVTENFNMWKNDMVE
QMHEDIISLWDQSLKPCVKLTPLCVSLKCTDLKNDTNTNSSSGRMIMEKGEIKNCSFN
ISTSIRDKVQKEYAFFYKLDIVPIDNTSYRLISCNTSVITQACPKVSFEPIPIHYCAP
AGFAILKCNNKTFNGTGPCTNVSTVQCTHGIRPVVSTQLLLNGSLAEEDVVIRSANFT
DNAKTIIVQLNTSVEINCTRPNNNTRKSIRIQRGPGRAFVTIGKIGNMRQAHCNISRA
KWNATLKQIASKLREQFGNNKTIIFKQSSGGDPEIVTHSFNCGGEFFYCNSTQLFNST
WFNSTWSTEGSNNTEGSDTITLPCRIKQFINMWQEVGKAMYAPPISGQIRCSSNITGL
LLTRDGGNNNNGSEIFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREK
RAVGIGALFLGFLGAAGSTMGAASMTLTVQARQLLSDIVQQQNNLLRAIEAQQHLLQL
TVWGIKQLQARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNNM
TWMEWDREINNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKL
FIMIVGGLVGLRIVFAVLSIVNRVRQGYSPLSFQTHLPIPRGPDRPEGIEEEGGERDR
DRSIRLVNGSLALIWDDLRSLCLFSYHRLRDLLLIVTRIVELLGRRGWEALKYWWNLL
QYWSQELKNSAVNLLNATAIAVAEGTDRVIEVLQAAYRAIRHIPRRIRQGLERILL"
CDS 8787..9407
/codon_start=1
/transl_table=11
/product="nef protein"
/protein_id="AAK08490.1"
/translation="MGGKWSKSSVIGWPAVRERMRRAEPAADGVGAVSRDLEKHGAIT
SSNTAANNAACAWLEAQEEEEVGFPVTPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIH
SQRRQDILDLWIYHTQGYFPDWQNYTPGPGVRYPLTFGWCYKLVPVEPDKVEEANKGE
NTSLLHPVSLHGMDDPEREVLEWRFDSRLAFHHVARELHPEYFKNC"
repeat_region 9076..9709
/note="3' long terminal repeat"
/rpt_type=long_terminal_repeat
repeat_region 9529..9626
/note="R; 3' copy"
regulatory 9602..9607
/regulatory_class="polyA_signal_sequence"
misc_feature 9710..11283
/note="3' cellular genomic sequence"
CDS complement(12279..13139)
/note="from pUC vector"
/codon_start=1
/transl_table=11
/product="beta-lactamase"
/protein_id="AAK08491.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
misc_feature 13648..14825
/note="5' cellular genomic sequence"
ORIGIN
1 tggaagggct aatttggtcc caaaaaagac aagagatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc agatatccac
121 tgacctttgg atggtgcttc aagttagtac cagttgaacc agagcaagta gaagaggcca
181 atgaaggaga gaacaacagc ttgttacacc ctatgagcca gcatgggatg gaggacccgg
241 agggagaagt attagtgtgg aagtttgaca gcctcctagc atttcgtcac atggcccgag
301 agctgcatcc ggagtactac aaagactgct gacatcgagc tttctacaag ggactttccg
361 ctggggactt tccagggagg tgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 gctacatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgctca aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagtaaa gccagaggag atctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcggta ttaagcgggg gagaattaga taaatgggaa
841 aaaattcggt taaggccagg gggaaagaaa caatataaac taaaacatat agtatgggca
901 agcagggagc tagaacgatt cgcagttaat cctggccttt tagagacatc agaaggctgt
961 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
1021 ttatataata caatagcagt cctctattgt gtgcatcaaa ggatagatgt aaaagacacc
1081 aaggaagcct tagataagat agaggaagag caaaacaaaa gtaagaaaaa ggcacagcaa
1141 gcagcagctg acacaggaaa caacagccag gtcagccaaa attaccctat agtgcagaac
1201 ctccaggggc aaatggtaca tcaggccata tcacctagaa ctttaaatgc atgggtaaaa
1261 gtagtagaag agaaggcttt cagcccagaa gtaataccca tgttttcagc attatcagaa
1321 ggagccaccc cacaagattt aaataccatg ctaaacacag tggggggaca tcaagcagcc
1381 atgcaaatgt taaaagagac catcaatgag gaagctgcag aatgggatag attgcatcca
1441 gtgcatgcag ggcctattgc accaggccag atgagagaac caaggggaag tgacatagca
1501 ggaactacta gtacccttca ggaacaaata ggatggatga cacataatcc acctatccca
1561 gtaggagaaa tctataaaag atggataatc ctgggattaa ataaaatagt aagaatgtat
1621 agccctacca gcattctgga cataagacaa ggaccaaagg aaccctttag agactatgta
1681 gaccgattct ataaaactct aagagccgag caagcttcac aagaggtaaa aaattggatg
1741 acagaaacct tgttggtcca aaatgcgaac ccagattgta agactatttt aaaagcattg
1801 ggaccaggag cgacactaga agaaatgatg acagcatgtc agggagtggg gggacccggc
1861 cataaagcaa gagttttggc tgaagcaatg agccaagtaa caaatccagc taccataatg
1921 atacagaaag gcaattttag gaaccaaaga aagactgtta agtgtttcaa ttgtggcaaa
1981 gaagggcaca tagccaaaaa ttgcagggcc cctaggaaaa agggctgttg gaaatgtgga
2041 aaggaaggac accaaatgaa agattgtact gagagacagg ctaatttttt agggaagatc
2101 tggccttccc acaagggaag gccagggaat tttcttcaga gcagaccaga gccaacagcc
2161 ccaccagaag agagcttcag gtttggggaa gagacaacaa ctccctctca gaagcaggag
2221 ccgatagaca aggaactgta tcctttagct tccctcagat cactctttgg cagcgacccc
2281 tcgtcacaat aaagataggg gggcaattaa aggaagctct attagataca ggagcagatg
2341 atacagtatt agaagaaatg aatttgccag gaagatggaa accaaaaatg atagggggaa
2401 ttggaggttt tatcaaagta agacagtatg atcagatact catagaaatc tgcggacata
2461 aagctatagg tacagtatta gtaggaccta cacctgtcaa cataattgga agaaatctgt
2521 tgactcagat tggctgcact ttaaattttc ccattagtcc tattgagact gtaccagtaa
2581 aattaaagcc aggaatggat ggcccaaaag ttaaacaatg gccattgaca gaagaaaaaa
2641 taaaagcatt agtagaaatt tgtacagaaa tggaaaagga aggaaaaatt tcaaaaattg
2701 ggcctgaaaa tccatacaat actccagtat ttgccataaa gaaaaaagac agtactaaat
2761 ggagaaaatt agtagatttc agagaactta ataagagaac tcaagatttc tgggaagttc
2821 aattaggaat accacatcct gcagggttaa aacagaaaaa atcagtaaca gtactggatg
2881 tgggcgatgc atatttttca gttcccttag ataaagactt caggaagtat actgcattta
2941 ccatacctag tataaacaat gagacaccag ggattagata tcagtacaat gtgcttccac
3001 agggatggaa aggatcacca gcaatattcc agtgtagcat gacaaaaatc ttagagcctt
3061 ttagaaaaca aaatccagac atagtcatct atcaatacat ggatgatttg tatgtaggat
3121 ctgacttaga aatagggcag catagaacaa aaatagagga actgagacaa catctgttga
3181 ggtggggatt taccacacca gacaaaaaac atcagaaaga acctccattc ctttggatgg
3241 gttatgaact ccatcctgat aaatggacag tacagcctat agtgctgcca gaaaaggaca
3301 gctggactgt caatgacata cagaaattag tgggaaaatt gaattgggca agtcagattt
3361 atgcagggat taaagtaagg caattatgta aacttcttag gggaaccaaa gcactaacag
3421 aagtagtacc actaacagaa gaagcagagc tagaactggc agaaaacagg gagattctaa
3481 aagaaccggt acatggagtg tattatgacc catcaaaaga cttaatagca gaaatacaga
3541 agcaggggca aggccaatgg acatatcaaa tttatcaaga gccatttaaa aatctgaaaa
3601 caggaaagta tgcaagaatg aagggtgccc acactaatga tgtgaaacaa ttaacagagg
3661 cagtacaaaa aatagccaca gaaagcatag taatatgggg aaagactcct aaatttaaat
3721 tacccataca aaaggaaaca tgggaagcat ggtggacaga gtattggcaa gccacctgga
3781 ttcctgagtg ggagtttgtc aatacccctc ccttagtgaa gttatggtac cagttagaga
3841 aagaacccat aataggagca gaaactttct atgtagatgg ggcagccaat agggaaacta
3901 aattaggaaa agcaggatat gtaactgaca gaggaagaca aaaagttgtc cccctaacgg
3961 acacaacaaa tcagaagact gagttacaag caattcatct agctttgcag gattcgggat
4021 tagaagtaaa catagtgaca gactcacaat atgcattggg aatcattcaa gcacaaccag
4081 ataagagtga atcagagtta gtcagtcaaa taatagagca gttaataaaa aaggaaaaag
4141 tctacctggc atgggtacca gcacacaaag gaattggagg aaatgaacaa gtagataaat
4201 tggtcagtgc tggaatcagg aaagtactat ttttagatgg aatagataag gcccaagaag
4261 aacatgagaa atatcacagt aattggagag caatggctag tgattttaac ctaccacctg
4321 tagtagcaaa agaaatagta gccagctgtg ataaatgtca gctaaaaggg gaagccatgc
4381 atggacaagt agactgtagc ccaggaatat ggcagctaga ttgtacacat ttagaaggaa
4441 aagttatctt ggtagcagtt catgtagcca gtggatatat agaagcagaa gtaattccag
4501 cagagacagg gcaagaaaca gcatacttcc tcttaaaatt agcaggaaga tggccagtaa
4561 aaacagtaca tacagacaat ggcagcaatt tcaccagtac tacagttaag gccgcctgtt
4621 ggtgggcggg gatcaagcag gaatttggca ttccctacaa tccccaaagt caaggagtaa
4681 tagaatctat gaataaagaa ttaaagaaaa ttataggaca ggtaagagat caggctgaac
4741 atcttaagac agcagtacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
4801 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
4861 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
4921 gagatccagt ttggaaagga ccagcaaagc tcctctggaa aggtgaaggg gcagtagtaa
4981 tacaagataa tagtgacata aaagtagtgc caagaagaaa agcaaagatc atcagggatt
5041 atggaaaaca gatggcaggt gatgattgtg tggcaagtag acaggatgag gattaacaca
5101 tggaaaagat tagtaaaaca ccatatgtat atttcaagga aagctaagga ctggttttat
5161 agacatcact atgaaagtac taatccaaaa ataagttcag aagtacacat cccactaggg
5221 gatgctaaat tagtaataac aacatattgg ggtctgcata caggagaaag agactggcat
5281 ttgggtcagg gagtctccat agaatggagg aaaaagagat atagcacaca agtagaccct
5341 gacctagcag accaactaat tcatctgcac tattttgatt gtttttcaga atctgctata
5401 agaaatacca tattaggacg tatagttagt cctaggtgtg aatatcaagc aggacataac
5461 aaggtaggat ctctacagta cttggcacta gcagcattaa taaaaccaaa acagataaag
5521 ccacctttgc ctagtgttag gaaactgaca gaggacagat ggaacaagcc ccagaagacc
5581 aagggccaca gagggagcca tacaatgaat ggacactaga gcttttagag gaacttaaga
5641 gtgaagctgt tagacatttt cctaggatat ggctccataa cttaggacaa catatctatg
5701 aaacttacgg ggatacttgg gcaggagtgg aagccataat aagaattctg caacaactgc
5761 tgtttatcca tttcagaatt gggtgtcgac atagcagaat aggcgttact cgacagagga
5821 gagcaagaaa tggagccagt agatcctaga ctagagccct ggaagcatcc aggaagtcag
5881 cctaaaactg cttgtaccaa ttgctattgt aaaaagtgtt gctttcattg ccaagtttgt
5941 ttcatgacaa aagccttagg catctcctat ggcaggaaga agcggagaca gcgacgaaga
6001 gctcatcaga acagtcagac tcatcaagct tctctatcaa agcagtaagt agtacatgta
6061 atgcaaccta taatagtagc aatagtagca ttagtagtag caataataat agcaatagtt
6121 gtgtggtcca tagtaatcat agaatatagg aaaatattaa gacaaagaaa aatagacagg
6181 ttaattgata gactaataga aagagcagaa gacagtggca atgagagtga aggagaagta
6241 tcagcacttg tggagatggg ggtggaaatg gggcaccatg ctccttggga tattgatgat
6301 ctgtagtgct acagaaaaat tgtgggtcac agtctattat ggggtacctg tgtggaagga
6361 agcaaccacc actctatttt gtgcatcaga tgctaaagca tatgatacag aggtacataa
6421 tgtttgggcc acacatgcct gtgtacccac agaccccaac ccacaagaag tagtattggt
6481 aaatgtgaca gaaaatttta acatgtggaa aaatgacatg gtagaacaga tgcatgagga
6541 tataatcagt ttatgggatc aaagcctaaa gccatgtgta aaattaaccc cactctgtgt
6601 tagtttaaag tgcactgatt tgaagaatga tactaatacc aatagtagta gcgggagaat
6661 gataatggag aaaggagaga taaaaaactg ctctttcaat atcagcacaa gcataagaga
6721 taaggtgcag aaagaatatg cattctttta taaacttgat atagtaccaa tagataatac
6781 cagctatagg ttgataagtt gtaacacctc agtcattaca caggcctgtc caaaggtatc
6841 ctttgagcca attcccatac attattgtgc cccggctggt tttgcgattc taaaatgtaa
6901 taataagacg ttcaatggaa caggaccatg tacaaatgtc agcacagtac aatgtacaca
6961 tggaatcagg ccagtagtat caactcaact gctgttaaat ggcagtctag cagaagaaga
7021 tgtagtaatt agatctgcca atttcacaga caatgctaaa accataatag tacagctgaa
7081 cacatctgta gaaattaatt gtacaagacc caacaacaat acaagaaaaa gtatccgtat
7141 ccagagggga ccagggagag catttgttac aataggaaaa ataggaaata tgagacaagc
7201 acattgtaac attagtagag caaaatggaa tgccacttta aaacagatag ctagcaaatt
7261 aagagaacaa tttggaaata ataaaacaat aatctttaag caatcctcag gaggggaccc
7321 agaaattgta acgcacagtt ttaattgtgg aggggaattt ttctactgta attcaacaca
7381 actgtttaat agtacttggt ttaatagtac ttggagtact gaagggtcaa ataacactga
7441 aggaagtgac acaatcacac tcccatgcag aataaaacaa tttataaaca tgtggcagga
7501 agtaggaaaa gcaatgtatg cccctcccat cagtggacaa attagatgtt catcaaatat
7561 tactgggctg ctattaacaa gagatggtgg taataacaac aatgggtccg agatcttcag
7621 acctggagga ggcgatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
7681 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
7741 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
7801 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctgatatagt
7861 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
7921 agtctggggc atcaaacagc tccaggcaag aatcctggct gtggaaagat acctaaagga
7981 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
8041 ttggaatgct agttggagta ataaatctct ggaacagatt tggaataaca tgacctggat
8101 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
8161 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
8221 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
8281 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
8341 gcagggatat tcaccattat cgtttcagac ccacctccca atcccgaggg gacccgacag
8401 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
8461 gaacggatcc ttagcactta tctgggacga tctgcggagc ctgtgcctct tcagctacca
8521 ccgcttgaga gacttactct tgattgtaac gaggattgtg gaacttctgg gacgcagggg
8581 gtgggaagcc ctcaaatatt ggtggaatct cctacagtat tggagtcagg aactaaagaa
8641 tagtgctgtt aacttgctca atgccacagc catagcagta gctgagggga cagatagggt
8701 tatagaagta ttacaagcag cttatagagc tattcgccac atacctagaa gaataagaca
8761 gggcttggaa aggattttgc tataagatgg gtggcaagtg gtcaaaaagt agtgtgattg
8821 gatggcctgc tgtaagggaa agaatgagac gagctgagcc agcagcagat ggggtgggag
8881 cagtatctcg agacctagaa aaacatggag caatcacaag tagcaataca gcagctaaca
8941 atgctgcttg tgcctggcta gaagcacaag aggaggaaga ggtgggtttt ccagtcacac
9001 ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
9061 aagaaaaggg gggactggaa gggctaattc actcccaaag aagacaagat atccttgatc
9121 tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag
9181 gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata
9241 aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg
9301 gaatggatga ccctgagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc
9361 atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgacat cgagcttgct
9421 acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt
9481 ggcgagccct cagatgctgc atataagcag ctgctttttg cctgtactgg gtctctctgg
9541 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
9601 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
9661 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcac ccaggaggta
9721 gaggttgcag tgagccaaga tcgcgccact gcattccagc ctgggcaaga aaacaagact
9781 gtctaaaata ataataataa gttaagggta ttaaatatat ttatacatgg aggtcataaa
9841 aatatatata tttgggctgg gcgcagtggc tcacacctgc gcccggccct ttgggaggcc
9901 gaggcaggtg gatcacctga gtttgggagt tccagaccag cctgaccaac atggagaaac
9961 cccttctctg tgtattttta gtagatttta ttttatgtgt attttattca caggtatttc
10021 tggaaaactg aaactgtttt tcctctactc tgataccaca agaatcatca gcacagagga
10081 agacttctgt gatcaaatgt ggtgggagag ggaggttttc accagcacat gagcagtcag
10141 ttctgccgca gactcggcgg gtgtccttcg gttcagttcc aacaccgcct gcctggagag
10201 aggtcagacc acagggtgag ggctcagtcc ccaagacata aacacccaag acataaacac
10261 ccaacaggtc caccccgcct gctgcccagg cagagccgat tcaccaagac gggaattagg
10321 atagagaaag agtaagtcac acagagccgg ctgtgcggga gaacggagtt ctattatgac
10381 tcaaatcagt ctccccaagc attcggggat cagagttttt aaggataact tagtgtgtag
10441 ggggccagtg agttggagat gaaagcgtag ggagtcgaag gtgtcctttt gcgccgagtc
10501 agttcctggg tgggggccac aagatcggat gagccagttt atcaatccgg gggtgccagc
10561 tgatccatgg agtgcagggt ctgcaaaata tctcaagcac tgattgatct taggttttac
10621 aatagtgatg ttaccccagg aacaatttgg ggaaggtcag aatcttgtag cctgtagctg
10681 catgactcct aaaccataat ttcttttttg tttttttttt tttatttttg agacagggtc
10741 tcactctgtc acctaggctg gagtgcagtg gtgcaatcac agctcactgc agcctcaacg
10801 tcgtaagctc aagcgatcct cccacctcag cctgcctggt agctgagact acaagcgacg
10861 ccccagttaa tttttgtatt tttggtagag gcagcgtttt gccgtgtggc cctggctggt
10921 ctcgaactcc tgggctcaag tgatccagcc tcagcctccc aaagtgctgg gacaaccggg
10981 gccagtcact gcacctggcc ctaaaccata atttctaatc ttttggctaa tttgttagtc
11041 ctacaaaggc agtctagtcc ccaggcaaaa agggggtttg tttcgggaaa gggctgttac
11101 tgtctttgtt tcaaactata aactaagttc ctcctaaact tagttcggcc tacacccagg
11161 aatgaacaag gagagcttgg aggttagaag cacgatggaa ttggttaggt cagatctctt
11221 tcactgtctg agttataatt ttgcaatggt ggttcaaaga ctgcccgctt ctgacaccag
11281 tcgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
11341 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
11401 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
11461 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
11521 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
11581 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
11641 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
11701 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
11761 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
11821 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
11881 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
11941 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
12001 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
12061 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
12121 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
12181 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
12241 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
12301 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
12361 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
12421 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
12481 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
12541 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
12601 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
12661 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
12721 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
12781 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
12841 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
12901 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
12961 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
13021 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
13081 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
13141 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
13201 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
13261 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
13321 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
13381 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
13441 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag
13501 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
13561 aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg
13621 gtgcgggcct cttcgctatt acgccagggg aggcagagat tgcagtaagc tgagatcgca
13681 gcactgcact ccagcctggg cgacagagta agactctgtc tcaaaaataa aataaataaa
13741 tcaatcagat attccaatct tttcctttat ttatttattt attttctatt ttggaaacac
13801 agtccttcct tattccagaa ttacacatat attctatttt tctttatatg ctccagtttt
13861 ttttagacct tcacctgaaa tgtgtgtata caaaatctag gccagtccag cagagcctaa
13921 aggtaaaaaa taaaataata aaaaataaat aaaatctagc tcactccttc acatcaaaat
13981 ggagatacag ctgttagcat taaataccaa ataacccatc ttgtcctcaa taattttaag
14041 cgcctctctc caccacatct aactcctgtc aaaggcatgt gccccttccg ggcgctctgc
14101 tgtgctgcca accaactggc atgtggactc tgcagggtcc ctaactgcca agccccacag
14161 tgtgccctga ggctgcccct tccttctagc ggctgccccc actcggcttt gctttcccta
14221 gtttcagtta cttgcgttca gccaaggtct gaaactaggt gcgcacagag cggtaagact
14281 gcgagagaaa gagaccagct ttacaggggg tttatcacag tgcaccctga cagtcgtcag
14341 cctcacaggg ggtttatcac attgcaccct gacagtcgtc agcctcacag ggggtttatc
14401 acagtgcacc cttacaatca ttccatttga ttcacaattt ttttagtctc tactgtgcct
14461 aacttgtaag ttaaatttga tcagaggtgt gttcccagag gggaaaacag tatatacagg
14521 gttcagtact atcgcatttc aggcctccac ctgggtcttg gaatgtgtcc cccgaggggt
14581 gatgactacc tcagttggat ctccacaggt cacagtgaca caagataacc aagacacctc
14641 ccaaggctac cacaatgggc cgccctccac gtgcacatgg ccggaggaac tgccatgtcg
14701 gaggtgcaag cacacctgcg catcagagtc cttggtgtgg agggagggac cagcgcagct
14761 tccagccatc cacctgatga acagaaccta gggaaagccc cagttctact tacaccagga
14821 aaggc
//
pNL4.3载体HIV-1载体图谱质粒图谱pdf版和相关资料下载
pNL4.3载体HIV-1载体质粒应用举例
NL4-3-ΔEnv-EGFP
2020-11-25pNL4-3-Luc-R-E-
2020-11-25
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