| 质粒名称: | pGADT7-GW |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 61702 |
| 存储实验室: | Yuhai Cui |
|
载体骨架基本信息 |
|
| 载体名称: | pGADT7 |
| 载体出品公司: | Clontech |
| 载体抗性: | 氯霉素和氨苄西林 |
| 空载体大小: | -- |
| 完整质粒大小: | 7988 bp |
| 载体修饰: | 网关磁带被克隆到MCS中 |
| 载体类型: | 酵母表达 |
| 筛选标记: | LEU2 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | Gateway克隆 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pGADT7-GW was a gift from Yuhai Cui (Addgene plasmid # 61702 ; http://n2t.net/addgene:61702 ; RRID:Addgene_61702) |
| 参考文献部分: | Arabidopsis homolog of the yeast TREX-2 mRNA export complex: components and anchoring nucleoporin. Lu Q, Tang X, Tian G, Wang F, Liu K, Nguyen V, Kohalmi SE, Keller WA, Tsang EW, Harada JJ, Rothstein SJ, Cui Y. Plant J. 2010 Jan;61(2):259-70. doi: 10.1111/j.1365-313X.2009.04048.x. Epub 2009 Oct 16. 10.1111/j.1365-313X.2009.04048.x PubMed 19843313 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pGADT7-GW 9638 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Gateway compatible Y2H vector - AD.
ACCESSION .
VERSION .
KEYWORDS pGADT7-GW
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9638)
AUTHORS Lu Q, Tang X, Tian G, Wang F, Liu K, Nguyen V, Kohalmi SE, Keller
WA, Tsang EW, Harada JJ, Rothstein SJ, Cui Y
TITLE Arabidopsis homolog of the yeast TREX-2 mRNA export complex:
components and anchoring nucleoporin.
JOURNAL Plant J. 2010 Jan;61(2):259-70. doi:
10.1111/j.1365-313X.2009.04048.x. Epub 2009 Oct 16.
PUBMED 19843313
REFERENCE 2 (bases 1 to 9638)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9638
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 771..1476
/gene="S. cerevisiae ADH1"
/label=ADH1 promoter
/note="promoter for alcohol dehydrogenase 1"
CDS 1522..1542
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 1558..1899
/codon_start=1
/gene="Saccharomyces cerevisiae GAL4 (truncated)"
/product="activation domain of the GAL4 transcriptional
activator"
/label=GAL4 activation domain
/translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
EDTPPNPKKE"
primer_bind 1905..1924
/label=T7
/note="T7 promoter, forward primer"
promoter 1905..1923
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
regulatory 1930..1939
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 1942..1968
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
protein_bind 1975..2099
/gene="mutant version of attR"
/label=attR1
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
promoter 2124..2154
/label=lac UV5 promoter
/note="E. coli lac promoter with an ""up"" mutation"
CDS 2208..2867
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
primer_bind complement(2274..2293)
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
CDS 3209..3514
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/label=ccdB
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
primer_bind 3393..3412
/label=ccdB-fwd
/note="ccdB gene, forward primer"
protein_bind complement(3555..3679)
/gene="mutant version of attR"
/label=attR2
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
terminator 4066..4253
/gene="S. cerevisiae ADH1"
/label=ADH1 terminator
/note="transcription terminator for the S. cerevisiae
alcohol dehydrogenase 1 (ADH1) gene"
primer_bind complement(4121..4145)
/label=pHybLex-R
/note="ADH terminator, reverse primer"
CDS complement(4370..5464)
/codon_start=1
/gene="S. cerevisiae LEU2"
/product="3-isopropylmalate dehydrogenase, required for
leucine biosynthesis"
/label=LEU2
/note="yeast auxotrophic marker"
/translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
IGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
DLGGSNSTTEVGDAVAEEVKKILA"
promoter complement(5465..5870)
/gene="S. cerevisiae LEU2"
/label=LEU2 promoter
primer_bind complement(5912..5928)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5912..5928)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(5925..5947)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 5936..5952
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5960..5990)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6005..6026
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
protein_bind complement(6081..6114)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
protein_bind complement(6192..6225)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
primer_bind complement(6294..6311)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6465..7053)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6545..6564)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7224..8084)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7847..7866
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8085..8189)
/gene="bla"
/label=AmpR promoter
primer_bind 8257..8275
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(8313..8335)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 8435..8454
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
rep_origin 8471..9635
/label=2u ori
/note="yeast 2u plasmid origin of replication"
ORIGIN
1 tgcatgcctg caggtcgaga tccgggatcg aagaaatgat ggtaaatgaa ataggaaatc
61 aaggagcatg aaggcaaaag acaaatataa gggtcgaacg aaaaataaag tgaaaagtgt
121 tgatatgatg tatttggctt tgcggcgccg aaaaaacgag tttacgcaat tgcacaatca
181 tgctgactct gtggcggacc cgcgctcttg ccggcccggc gataacgctg ggcgtgaggc
241 tgtgcccggc ggagtttttt gcgcctgcat tttccaaggt ttaccctgcg ctaaggggcg
301 agattggaga agcaataaga atgccggttg gggttgcgat gatgacgacc acgacaactg
361 gtgtcattat ttaagttgcc gaaagaacct gagtgcattt gcaacatgag tatactagaa
421 gaatgagcca agacttgcga gacgcgagtt tgccggtggt gcgaacaata gagcgaccat
481 gaccttgaag gtgagacgcg cataaccgct agagtacttt gaagaggaaa cagcaatagg
541 gttgctacca gtataaatag acaggtacat acaacactgg aaatggttgt ctgtttgagt
601 acgctttcaa ttcatttggg tgtgcacttt attatgttac aatatggaag ggaactttac
661 acttctccta tgcacatata ttaattaaag tccaatgcta gtagagaagg ggggtaacac
721 ccctccgcgc tcttttccga tttttttcta aaccgtggaa tatttcggat atccttttgt
781 tgtttccggg tgtacaatat ggacttcctc ttttctggca accaaaccca tacatcggga
841 ttcctataat accttcgttg gtctccctaa catgtaggtg gcggagggga gatatacaat
901 agaacagata ccagacaaga cataatgggc taaacaagac tacaccaatt acactgcctc
961 attgatggtg gtacataacg aactaatact gtagccctag acttgatagc catcatcata
1021 tcgaagtttc actacccttt ttccatttgc catctattga agtaataata ggcgcatgca
1081 acttcttttc tttttttttc ttttctctct cccccgttgt tgtctcacca tatccgcaat
1141 gacaaaaaaa atgatggaag acactaaagg aaaaaattaa cgacaaagac agcaccaaca
1201 gatgtcgttg ttccagagct gatgaggggt atctcgaagc acacgaaact ttttccttcc
1261 ttcattcacg cacactactc tctaatgagc aacggtatac ggccttcctt ccagttactt
1321 gaatttgaaa taaaaaaaag tttgctgtct tgctatcaag tataaataga cctgcaatta
1381 ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt ctttttctgc
1441 acaatatttc aagctatacc aagcatacaa tcaactccaa gctttgcaaa gatggataaa
1501 gcggaattaa ttcccgagcc tccaaaaaag aagagaaagg tcgaattggg taccgccgcc
1561 aattttaatc aaagtgggaa tattgctgat agctcattgt ccttcacttt cactaacagt
1621 agcaacggtc cgaacctcat aacaactcaa acaaattctc aagcgctttc acaaccaatt
1681 gcctcctcta acgttcatga taacttcatg aataatgaaa tcacggctag taaaattgat
1741 gatggtaata attcaaaacc actgtcacct ggttggacgg accaaactgc gtataacgcg
1801 tttggaatca ctacagggat gtttaatacc actacaatgg atgatgtata taactatcta
1861 ttcgatgatg aagatacccc accaaaccca aaaaaagaga tctttaatac gactcactat
1921 agggcgagcg ccgccatgga gtacccatac gacgtaccag attacgctca tatgacaagt
1981 ttgtacaaaa aagctgaacg agaaacgtaa aatgatataa atatcaatat attaaattag
2041 attttgcata aaaaacagac tacataatac tgtaaaacac aacatatcca gtcactatgg
2101 cggccgcatt aggcacccca ggctttacac tttatgcttc cggctcgtat aatgtgtgga
2161 ttttgagtta ggatccgtcg agattttcag gagctaagga agctaaaatg gagaaaaaaa
2221 tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat
2281 ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt
2341 taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc
2401 gcctgatgaa tgctcatccg gaattccgta tggcaatgaa agacggtgag ctggtgatat
2461 gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc
2521 tctggagtga ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg
2581 cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg
2641 tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca
2701 acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga
2761 tgccgctggc gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc
2821 ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaaacg cgtggatccg
2881 gcttactaaa agccagataa cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa
2941 tatatactga tatgtatacc cgaagtatgt caaaaagagg tatgctatga agcagcgtat
3001 tacagtgaca gttgacagcg acagctatca gttgctcaag gcatatatga tgtcaatatc
3061 tccggtctgg taagcacaac catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg
3121 aaagcggaaa atcaggaagg gatggctgag gtcgcccggt ttattgaaat gaacggctct
3181 tttgctgacg agaacagggg ctggtgaaat gcagtttaag gtttacacct ataaaagaga
3241 gagccgttat cgtctgtttg tggatgtaca gagtgatatt attgacacgc ccgggcgacg
3301 gatggtgatc cccctggcca gtgcacgtct gctgtcagat aaagtctccc gtgaacttta
3361 cccggtggtg catatcgggg atgaaagctg gcgcatgatg accaccgata tggccagtgt
3421 gccggtctcc gttatcgggg aagaagtggc tgatctcagc caccgcgaaa atgacatcaa
3481 aaacgccatt aacctgatgt tctggggaat ataaatgtca ggctccctta tacacagcca
3541 gtctgcaggt cgaccatagt gactggatat gttgtgtttt acagtattat gtagtctgtt
3601 ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt tctcgttcag
3661 ctttcttgta caaagtggtc tcgagctgca gatgaatcgt agatactgaa aaaccccgca
3721 agttcacttc aactgtgcat cgtgcaccat ctcaatttct ttcatttata catcgttttg
3781 ccttctttta tgtaactata ctcctctaag tttcaatctt ggccatgtaa cctctgatct
3841 atagaatttt ttaaatgact agaattaatg cccatctttt ttttggacct aaattcttca
3901 tgaaaatata ttacgagggc ttattcagaa gctttggact tcttcgccag aggtttggtc
3961 aagtctccaa tcaaggttgt cggcttgtct accttgccag aaatttacga aaagatggaa
4021 aagggtcaaa tcgttggtag atacgttgtt gacacttcta aataagcgaa tttcttatga
4081 tttatgattt ttattattaa ataagttata aaaaaaataa gtgtatacaa attttaaagt
4141 gactcttagg ttttaaaacg aaaattctta ttcttgagta actctttcct gtaggtcagg
4201 ttgctttctc aggtatagca tgaggtcgct cttattgacc acacctctac cggccggtcg
4261 aaattcccct accctatgaa catattccat tttgtaattt cgtgtcgttt ctattatgaa
4321 tttcatttat aaagtttatg tacaaatatc ataaaaaaag agaatctttt taagcaagga
4381 ttttcttaac ttcttcggcg acagcatcac cgacttcggt ggtactgttg gaaccaccta
4441 aatcaccagt tctgatacct gcatccaaaa cctttttaac tgcatcttca atggccttac
4501 cttcttcagg caagttcaat gacaatttca acatcattgc agcagacaag atagtggcga
4561 tagggttgac cttattcttt ggcaaatctg gagcagaacc gtggcatggt tcgtacaaac
4621 caaatgcggt gttcttgtct ggcaaagagg ccaaggacgc agatggcaac aaacccaagg
4681 aacctgggat aacggaggct tcatcggaga tgatatcacc aaacatgttg ctggtgatta
4741 taataccatt taggtgggtt gggttcttaa ctaggatcat ggcggcagaa tcaatcaatt
4801 gatgttgaac cttcaatgta ggaaattcgt tcttgatggt ttcctccaca gtttttctcc
4861 ataatcttga agaggccaaa acattagctt tatccaagga ccaaataggc aatggtggct
4921 catgttgtag ggccatgaaa gcggccattc ttgtgattct ttgcacttct ggaacggtgt
4981 attgttcact atcccaagcg acaccatcac catcgtcttc ctttctctta ccaaagtaaa
5041 tacctcccac taattctctg acaacaacga agtcagtacc tttagcaaat tgtggcttga
5101 ttggagataa gtctaaaaga gagtcggatg caaagttaca tggtcttaag ttggcgtaca
5161 attgaagttc tttacggatt tttagtaaac cttgttcagg tctaacacta cctgtacccc
5221 atttaggacc acccacagca cctaacaaaa cggcatcaac cttcttggag gcttccagcg
5281 cctcatctgg aagtgggaca cctgtagcgt cgatagcagc accaccaatt aaatgatttt
5341 cgaaatcgaa cttgacattg gaacgaacat cagaaatagc tttaagaacc ttaatggctt
5401 cggctgtgat ttcttgacca acgtggtcac ctggcaaaac gacgatcttc ttaggggcag
5461 acattagaat ggtatatcct tgaaatatat atatatattg ctgaaatgta aaaggtaaga
5521 aaagttagaa agtaagacga ttgctaacca cctattggaa aaaacaatag gtccttaaat
5581 aatattgtca acttcaagta ttgtgatgca agcatttagt catgaacgct tctctattct
5641 atatgaaaag ccggttccgg cgctctcacc tttccttttt ctcccaattt ttcagttgaa
5701 aaaggtatat gcgtcaggcg acctctgaaa ttaacaaaaa atttccagtc atcgaatttg
5761 attctgtgcg atagcgcccc tgtgtgttct cgttatgttg aggaaaaaaa taatggttgc
5821 taagagattc gaactcttgc atcttacgat acctgagtat tcccacagtt ggggatctcg
5881 actctagcta gaggatcaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
5941 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
6001 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
6061 gaaacctgtc gtgccagctg ataacttcgt ataatgtatg ctatacgaag ttattaggtc
6121 tgaagaggag tttacgtcca gccaagctag cttggctgca ggtcgagcgg ccgcgatccg
6181 gaacccttaa tataacttcg tataatgtat gctatacgaa gttatcagct gcattaatga
6241 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
6301 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
6361 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
6421 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
6481 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
6541 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
6601 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
6661 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
6721 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
6781 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
6841 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
6901 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
6961 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
7021 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
7081 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
7141 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
7201 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
7261 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
7321 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
7381 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
7441 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
7501 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
7561 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
7621 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
7681 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
7741 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
7801 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
7861 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
7921 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
7981 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
8041 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
8101 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
8161 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
8221 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
8281 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
8341 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
8401 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga
8461 gtgcaccata acgcatttaa gcataaacac gcactatgcc gttcttctca tgtatatata
8521 tatacaggca acacgcagat ataggtgcga cgtgaacagt gagctgtatg tgcgcagctc
8581 gcgttgcatt ttcggaagcg ctcgttttcg gaaacgcttt gaagttccta ttccgaagtt
8641 cctattctct agctagaaag tataggaact tcagagcgct tttgaaaacc aaaagcgctc
8701 tgaagacgca ctttcaaaaa accaaaaacg caccggactg taacgagcta ctaaaatatt
8761 gcgaataccg cttccacaaa cattgctcaa aagtatctct ttgctatata tctctgtgct
8821 atatccctat ataacctacc catccacctt tcgctccttg aacttgcatc taaactcgac
8881 ctctacattt tttatgttta tctctagtat tactctttag acaaaaaaat tgtagtaaga
8941 actattcata gagtgaatcg aaaacaatac gaaaatgtaa acatttccta tacgtagtat
9001 atagagacaa aatagaagaa accgttcata attttctgac caatgaagaa tcatcaacgc
9061 tatcactttc tgttcacaaa gtatgcgcaa tccacatcgg tatagaatat aatcggggat
9121 gcctttatct tgaaaaaatg cacccgcagc ttcgctagta atcagtaaac gcgggaagtg
9181 gagtcaggct ttttttatgg aagagaaaat agacaccaaa gtagccttct tctaacctta
9241 acggacctac agtgcaaaaa gttatcaaga gactgcatta tagagcgcac aaaggagaaa
9301 aaaagtaatc taagatgctt tgttagaaaa atagcgctct cgggatgcat ttttgtagaa
9361 caaaaaagaa gtatagattc tttgttggta aaatagcgct ctcgcgttgc atttctgttc
9421 tgtaaaaatg cagctcagat tctttgtttg aaaaattagc gctctcgcgt tgcatttttg
9481 ttttacaaaa atgaagcaca gattcttcgt tggtaaaata gcgctttcgc gttgcatttc
9541 tgttctgtaa aaatgcagct cagattcttt gtttgaaaaa ttagcgctct cgcgttgcat
9601 ttttgttcta caaaatgaag cacagatgct tcgttgct
//
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