| 质粒名称: | pHRW32 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 130972 |
| 存储实验室: | Chris Todd Hittinger |
|
载体骨架基本信息 |
|
| 载体名称: | pHCT2 |
| 载体出品公司: | -- |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 酵母表达 |
| 筛选标记: | 博莱霉素 |
| 载体抗性: | 氨苄霉素100 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | empty backbone |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | -- |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pHRW32 was a gift from Chris Todd Hittinger (Addgene plasmid # 130972 ; http://n2t.net/addgene:130972 ; RRID:Addgene_130972) |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pHRW32 10858 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION HyPr plasmid with ble marker.
ACCESSION .
VERSION .
KEYWORDS pHRW32
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10858)
TITLE Allododecaploid yeasts: synthetic hybrids of six species
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 10858)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10858
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 1265..1276
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
CDS 1922..2419
/codon_start=1
/product="13 tandem Myc epitope tags"
/label=13xMyc
/translation="EQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLIS
EEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLD
GEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISE
EDL"
CDS 2039..2536
/codon_start=1
/product="13 tandem Myc epitope tags"
/label=13xMyc
/translation="EQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLIS
EEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLD
GEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISE
EDL"
terminator 2612..2859
/gene="S. cerevisiae CYC1"
/label=CYC1 terminator
/note="transcription terminator for CYC1"
primer_bind complement(2628..2646)
/label=CYC1
/note="CYC1 transcription termination signal, reverse
primer"
primer_bind complement(2882..2898)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2882..2898)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(2895..2917)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 2906..2922
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(2930..2960)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2975..2996
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(3113..3130)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(3284..3872)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(3364..3383)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(4043..4903)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 4666..4685
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(4904..5008)
/gene="bla"
/label=AmpR promoter
primer_bind 5076..5094
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 5216..5559
/label=TEF promoter
/note="Ashbya gossypii TEF promoter"
terminator 5940..6137
/label=TEF terminator
/note="Ashbya gossypii TEF terminator"
rep_origin 6480..7317
/label=ARS1
/note="S. cerevisiae autonomously replicating sequence
ARS1/ARS416"
misc_feature 7453..7745
/label=CEN4
/note="S. cerevisiae CEN4 centromere"
primer_bind 8487..8509
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 8501..8518
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 8502..8518
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
CDS complement(8546..9553)
/codon_start=1
/product="tetracycline-controlled transactivator,
comprising a fusion of the tetracycline repressor TetR with
the C-terminal activation domain of herpes simplex virus
VP16"
/label=tTA
/note="In the Tet-Off(R) system, tTA binds to the
Tet-responsive element and stimulates transcription only in
the absence of tetracycline or doxycycline."
/translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGSAYSRARTKNNYGSTI
EGLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAHA
DALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
primer_bind 9461..9480
/label=Tet-R
/note="Tetracycline resistance gene, reverse primer"
primer_bind complement(9638..9662)
/label=LNCX
/note="Human CMV promoter, forward primer"
promoter complement(9659..9862)
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind complement(9688..9708)
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
enhancer 9863..10242
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
primer_bind complement(10407..10431)
/label=pHybLex-R
/note="ADH terminator, reverse primer"
protein_bind 10597..10615
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 10639..10657
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 10681..10699
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 10723..10741
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 10765..10783
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
1 tctctaaata ttctttcctt atacattagg tcctttgtag cataaattac tatacttcta
61 tagacacgca aacacaaata cacacactaa attaccggat caattcgggg gaaaaatgct
121 ttctgaaaac acgactattc tgatggctaa cggtgaaatt aaagacatcg caaacgtcac
181 ggctaactct tacgttatgt gcgcagatgg ctccgctgcc cgcgtcataa atgtcacaca
241 gggctatcag aaaatctata atatacagca aaaaaccaaa cacagagctt ttgaaggtga
301 acctggtagg ttagatccca ggcgtagaac agtttatcag cgtcttgcat tacaatgtac
361 tgcaggtcat aaattgtcag tcagggtccc taccaaacca ctgttggaaa aaagtggtag
421 aaatgccacc aaatataaag tgagatggag aaatctgcag caatgtcaga cgcttgatgg
481 taggataata ataattccaa aaaaccatca taagacattc ccaatgacag ttgaaggtga
541 gtttgccgca aaacgcttca tagaagaaat ggagcgctct aaaggagaat atttcaactt
601 tgacattgaa gttagagatt tggattatct tgatgctcaa ttgagaattt ctagctgcat
661 aagatttggt ccagtactca caggaaatgg tgttttatct aaatttctca ctggacgtag
721 tgaccttgta actcctgctg taaaaagtat ggcttggatg cttggtctgt ggttaggtga
781 cggtacaaca aaagagccag aaatctcagt agatagcttg gatcctaagc taatggagag
841 tttaagagaa aatgcgaaaa tctggggtct ctaccttacg gtttgtgacg atcacgttcc
901 gctacgtgcc aaacatgtaa ggcttcatta tggagatggt ccagatgaaa acaggaagac
961 aaggaatttg aggaaaaata atccattctg gaaagctgtc acaattttaa agtttaaaag
1021 ggatcttgat ggagagaagc aaatccctga atttatgtac ggcgagcata tagaagttcg
1081 tgaagcattc ttagccggct tgatcgactc agatgggtac gttgtgaaaa agggcgaagg
1141 ccctgaatct tataaaatag caattcaaac tgtttattca tccattatgg acggaattgt
1201 ccatatttca agatctcttg gtatgtcagc tactgtgacg accaggtcag ctagggagga
1261 aatcattgaa ggaagaaaag tccaatgtca atttacatac gactgtaatg ttgctggggg
1321 aacaacttta cagaatgttt tgtcatattg tcgaagtggt cacaaaacaa gagaagttcc
1381 gccaattata aaaagggaac ccgtatattt cagcttcacg gatgatttcc agggtgagag
1441 tactgtatat gggcttacga tagaaggcca taaaaatttc ttgcttggca acaaaataga
1501 agtgaaatca tgtcgaggct gctgtgtggg agaacagcat aaaatatcac aaaaaaagaa
1561 tctaaaacac tgtgttgctt gtcccagaaa gggaatcaag tatttttata aagattggag
1621 tggtaaaaat cgagtatgtg ctagatgcta tggaagatac aaattcagcg gtcatcactg
1681 tataaattgc aagtatgtac cagaagcacg tgaagtgaaa aaggcaaaag acaaaggcga
1741 aaaattgggc attacgcccg aaggtttgcc agttaaagga ccagagtgta taaaatgtgg
1801 cggaatctta cagtttgatg ctgtccgcgg gcctcataag agttgtggta acaacgcagg
1861 tgcgcgcatc tgccggatcc ccgggttaat taacggtgga ggtggaggtg gaggtggagg
1921 tgaacaaaag ctaatctccg aggaagactt gaacggtgaa caaaaattaa tctcagaaga
1981 agacttgaac ggactcgacg gtgaacaaaa gttgatttct gaagaagatt tgaacggtga
2041 acaaaagcta atctccgagg aagacttgaa cggtgaacaa aaattaatct cagaagaaga
2101 cttgaacgga ctcgacggtg aacaaaagtt gatttctgaa gaagatttga acggtgaaca
2161 aaagctaatc tccgaggaag acttgaacgg tgaacaaaaa ttaatctcag aagaagactt
2221 gaacggactc gacggtgaac aaaagttgat ttctgaagaa gatttgaacg gtgaacaaaa
2281 gctaatctcc gaggaagact tgaacggtga acaaaaatta atctcagaag aagacttgaa
2341 cggactcgac ggtgaacaaa agttgatttc tgaagaagat ttgaacggtg aacaaaagct
2401 aatctccgag gaagacttga acggtgaaca aaaattaatc tcagaagaag acttgaacgg
2461 actcgacggt gaacaaaagt tgatttctga agaagatttg aacggtgaac aaaagctaat
2521 ctccgaggaa gacttgaacg gtgaacaaaa attaatcaat cactagtgaa ttcgcgccac
2581 ttctaaataa gcgaatttct tgagggccgc atcatgtaat tagttatgtc acgcttacat
2641 tcacgccctc cccccacatc cgctctaacc gaaaaggaag gagttagaca acctgaagtc
2701 taggtcccta tttatttttt tatagttatg ttagtattaa gaacgttatt tatatttcaa
2761 atttttcttt tttttctgta cagacgcgtg tacgcatgta acattatact gaaaaccttg
2821 cttgagaagg ttttgggacg ctcgaaggct ttaatttgcg gccaagcttg gcgtaatcat
2881 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
2941 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
3001 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
3061 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
3121 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
3181 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
3241 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
3301 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
3361 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
3421 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
3481 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
3541 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
3601 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
3661 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
3721 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
3781 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
3841 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
3901 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
3961 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
4021 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
4081 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
4141 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
4201 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
4261 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
4321 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
4381 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
4441 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
4501 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
4561 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
4621 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
4681 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
4741 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
4801 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
4861 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
4921 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
4981 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
5041 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
5101 gtcttcaaga attcagctga agcttcgtac gctgcaggtc gacggatccc cgggttaatt
5161 aaggcgcgcc agatctgttt agcttgcctc gtccccgccg ggtcacccgg ccagcgacat
5221 ggaggcccag aataccctcc ttgacagtct tgacgtgcgc agctcagggg catgatgtga
5281 ctgtcgcccg tacatttagc ccatacatcc ccatgtataa tcatttgcat ccatacattt
5341 tgatggccgc acggcgcgaa gcaaaaatta cggctcctcg ctgcagacct gcgagcaggg
5401 aaacgctccc ctcacagacg cgttgaattg tccccacgcc gcgcccctgt agagaaatat
5461 aaaaggttag gatttgccac tgaggttctt ctttcatata cttcctttta aaatcttgct
5521 aggatacagt tctcacatca catccgaaca taaacaacca tggctaagtt gacctctgct
5581 gttccagttt tgactgctag agatgttgct ggtgctgttg aattttggac cgacagattg
5641 ggtttttcca gagatttcgt tgaagacgat ttcgctggtg ttgttcgtga cgatgttacc
5701 ttgttcatct ccgccgttca agatcaagtt gttccagata atactttggc ttgggtttgg
5761 gttagaggtt tagacgagtt gtatgctgaa tggtctgaag tcgtttctac taacttcaga
5821 gatgcttctg gtccagctat gaccgaaatt ggtgaacaac catggggtag agaatttgcc
5881 ttgagagacc cagccggtaa ttgtgttcat ttcgtcgccg aagaacaaga ttgatcagta
5941 ctgacaataa aaagattctt gttttcaaga acttgtcatt tgtatagttt ttttatattg
6001 tagttgttct attttaatca aatgttagcg tgatttatat tttttttcgc ctcgacatca
6061 tctgcccaga tgcgaagtta agtgcgcaga aagtaatatc atgcgtcaat cgtatgtgaa
6121 tgctggtcgc tatactgctg tcgattcgat actaacgccg ccatccagtg tcgaaaacga
6181 gctcgaattc atcgatgata tcagatccac tagtggccta tgcaattctc atgtttgaca
6241 gcttatcatc gatcgtccaa ctgcatggag atgagtcgtg gcaagaatac caagagttcc
6301 tcggtttgcc agttattaaa agactcgtat ttccaaaaga ctgcaacata ctactcagtg
6361 cagcttcaca gaaacctcat tcgtttattc ccttgtttga ttcagaagca ggtgggacag
6421 gtgaactttt ggattggaac tcgatttctg actgggttgg aaggcaagag agccccgaga
6481 gcttacattt tatgttagct ggtggactga cgccagaaaa tgttggtgat gcgcttagat
6541 taaatggcgt tattggtgtt gatgtaagcg gaggtgtgga gacaaatggt gtaaaagact
6601 ctaacaaaat agcaaatttc gtcaaaaatg ctaagaaata ggttattact gagtagtatt
6661 tatttaagta ttgtttgtgc acttgcctgc aagccttttg aaaagcaagc ataaaagatc
6721 taaacataaa atctgtaaaa taacaagatg taaagataat gctaaatcat ttggcttttt
6781 gattgattgt acaggaaaat atacatcgca gggggttgac ttttaccatt tcaccgcaat
6841 ggaatcaaac ttgttgaaga gaatgttcac aggcgcatac gctacaatga cccgattctt
6901 gctagccttt tctcggtctt gcaaacaacc gccggcagct tagtatataa atacacatgt
6961 acatacctct ctccgtatcc tcgtaatcat tttcttgtat ttatcgtctt ttcgctgtaa
7021 aaactttatc acacttatct caaatacact tattaaccgc ttttactatt atcttctacg
7081 ctgacagtaa tatcaaacag tgacacatat taaacacagt ggtttctttg cataaacacc
7141 atcagcctca agtcgtcaag taaagatttc gtgttcatgc agatagataa caatctatat
7201 gttgataatt agcgttgcct catcaatgcg agatccgttt aaccggaccc tagtgcactt
7261 accccacgtt cggtccactg tgtgccgaac atgctccttc actattttaa catgtggaat
7321 tatcgatgcc ggtgttattc ttgctatgat aggccatcga atgttaaagg gaagatttat
7381 tgttttgccc tttaaagacg aatttggacc gtgaatgttt aaataattct catgtttgac
7441 agcttatcat cgaactctaa gaggtgatac ttatttactg taaaactgtg acgataaaac
7501 cggaaggaag aataagaaaa ctcgaactga tctataatgc ctattttctg taaagagttt
7561 aagctatgaa agcctcggca ttttggccgc tcctaggtag tgcttttttt ccaaggacaa
7621 aacagtttct ttttcttgag caggttttat gtttcggtaa tcataaacaa taaataaatt
7681 atttcattta tgtttaaaaa taaaaaataa aaaagtattt taaattttta aaaaagttga
7741 ttataagcat gtgacctttt gcaagcaatt aaattttgca atttgtgatt taggcaaaag
7801 ttactatttc tggctcgtgt aatatatgta tgctaatgtg aacttttaca aagtcgatat
7861 ggacttagtc aaaagaaatt ttcttaaaaa tatatagcac tagccaattt agcacttctt
7921 tatgagatat attatagact ttattaagcc agatttgtgt attatatgta tttacccggc
7981 gaatcatgga catacattct gaaataggta atattctcta tggtgagaca gcatagataa
8041 cctaggatac aagttaaaag ctagtactgt tttgcagtaa tttttttctt ttttataaga
8101 atgttaccac ctaaataagt tataaagtca atagttaagt ttgatatttg attgtaaaat
8161 accgtaatat atttgcatga tcaaaaggct caatgttgac tagccagcat gtcaaccact
8221 atattgatca ccgatattag gacttccaca ccaactagta atatgacaat aaattcaaga
8281 tattcttcat gagaatggcc cagcgattat gcggtgtgaa ataccgcaca gatgcgtaag
8341 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg
8401 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
8461 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
8521 attcttatta cgatcctcgc gccccctacc caccgtactc gtcaattcca agggcatcgg
8581 taaacatctg ctcaaactcg aagtcggcca tatccagagc gccgtagggg gcggagtcgt
8641 ggggggtaaa tcccggaccc ggggaatccc cgtcccccaa catgtccaga tcgaaatcgt
8701 ctagcgcgtc ggcatgcgcc atcgccacgt cctcgccgtc taagtggagc tcgtccccca
8761 ggctgacatc ggtcgggggg gccgtcgaca gtctgcgcgt gtgtcccgcg gggagaaagg
8821 acaggcgcgg agccgccagc cccgcctctt cgggggcgtc gtcgtccggg agatcgagca
8881 ggccctcgat ggtagacccg taattgtttt tcgtacgcgc gcggctgtac gcggacccac
8941 tttcacattt aagttgtttt tctaatccgc atatgatcaa ttcaaggccg aataagaagg
9001 ctggctctgc accttggcga tcaaataatt cgatagcttg tcgtaataat ggcggcatac
9061 tatcagtagt aggtgtttcc ctttcttctt tagcgacttg atgctcttgt tcttccaata
9121 cgcaacctaa agtaaagtgc cccacagcgc tgagtgcata taatgcattc tctagtgaaa
9181 aaccttgttg gcataaaaag gctaattgat tttcgagagt ttcatactgt ttttctgtag
9241 gccgtgtacc taaatgtact tttgctccat cgcgatgact tagtaaagca catctaaaac
9301 ttttagcgtt attacgtaaa aaatcttgcc agctttcccc ttctaaaggg caaaagtgag
9361 tatggtgcct atctaacatc tcaatgggta aggcgtcgag caaagcccgc ttatttttta
9421 catgccaata caatgtaggc tgctctacac ctagcttctg ggcgagttta cgggttgtta
9481 aaccttcgat tccgacccca ttaagcagct ctaatgcgcc gttaatcact ttacttttat
9541 ctaatctaga catatgaatt aattcggggc cgcggaggct ggatcggtcc cggtgtcttc
9601 tatggaggtc aaaacagcgt ggatggcgtc tccaggcgat ctgacggttc actaaacgag
9661 ctctgcttat atagacctcc caccgtacac gcctaccgcc catttgcgtc aatggggcgg
9721 agttgttacg acattttgga aagtcccgtt gattttggtg ccaaaacaaa ctcccattga
9781 cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga
9841 tgtactgcca aaaccgcatc accatggtaa tagcgatgac taatacgtag atgtactgcc
9901 aagtaggaaa gtcccataag gtcatgtact gggcataatg ccaggcgggc catttaccgt
9961 cattgacgtc aatagggggc gtacttggca tatgatacac ttgatgtact gccaagtggg
10021 cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt ggcgttacta
10081 tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg
10141 ggccatttac cgtaagttat gtaacgcgga actccatata tgggctatga actaatgacc
10201 ccgtaattga ttactattaa taactagtca ataatcaatg tcaacatggc ggtaatgttg
10261 gacatgagcc aatataaatg tacatattat gatatggata caacgtatgc aatgggccaa
10321 gctcctcgag taattcgcgc cacttctaat aagcgatttc ttatgattta tgatttttat
10381 tattaaataa gttataaaaa aaataagtgt atacaaattt taaagtgact cttaggtttt
10441 aaaacgaaaa ttcttattct tgagtaactc tttcctgtag gtcaggttgc tttctcaggt
10501 atagtatgag gtcgctctta ttgaccacac ctctaccggc agatccgcta gggataacag
10561 ggtaatatag atcaattcct cgatcgagtt taccactccc tatcagtgat agagaaaagt
10621 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
10681 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
10741 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagct
10801 cggtacccta tggcatgcat gtgctctgta tgtatataaa actcttgttt tcttcttt
//
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