pIRES-EGFP

价格:800元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Clontech
载体名称: pIRES-EGFP, pIRES EGFP
质粒类型: 哺乳动物细胞表达载体;双顺反子载体;荧光报告载体
启动子: CMV
表达水平:
克隆方法: 多克隆位点,限制性内切酶
载体大小: 5162 bp
5' 测序引物:   CMV-F: 5'-CGCAAATGGGCGGTAGGCGTG-3'
3' 测序引物: EGFP-N: 5'-CGTCGCCGTCCAGCTCGACCAG-3'
载体标签: EGFP
载体抗性: 氨苄青霉素
筛选标记: --
备注:
通过一个转录mRNA,能够同时表达EGFP蛋白和一个目的基因。
产品目录号: 6064-1
稳定性: 稳表达 或 瞬表达
组成型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pIRES-EGFP载体图谱



pIRES-EGFP多克隆位点

pIRES-EGFP载体特征

载体简介

载体序列

LOCUS       pIRES-EGFP              5162 bp    DNA     circular     16-FEB-2014
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     promoter        232..820
                     /vntifkey="29"
                     /label=CMV\Promoter
     misc_feature    909..974
                     /vntifkey="21"
                     /label=MCS
     intron          974..1269
                     /vntifkey="15"
                     /label=Synthetic\Intron\(IVS)
     RBS             1299..1884
                     /vntifkey="32"
                     /label=IRES
     CDS             1905..2621
                     /vntifkey="4"
                     /label=EGFP
     polyA_signal    2636..2913
                     /vntifkey="25"
                     /label=poly\A
     rep_origin      3343..4016
                     /vntifkey="33"
                     /label=Col\E1\Origin
     CDS             complement(4168..5026)
                     /vntifkey="4"
                     /label=Ampcillin
BASE COUNT     1237 a      1371 c      1325 g      1229 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc 
      901 gagctcggat cgatatctgc ggccgcgtcg acggaattca gtggatccac tagtaacggc 
      961 cgccagtgtg ctggaattaa ttcgctgtct gcgagggcca gctgttgggg tgagtactcc 
     1021 ctctcaaaag cgggcatgac ttctgcgcta agattgtcag tttccaaaaa cgaggaggat 
     1081 ttgatattca cctggcccgc ggtgatgcct ttgagggtgg ccgcgtccat ctggtcagaa 
     1141 aagacaatct ttttgttgtc aagcttgagg tgtggcaggc ttgagatctg gccatacact 
     1201 tgagtgacaa tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaac 
     1261 tgcaggtcga gcatgcatct agggcggcca attccgcccc tctccctccc ccccccctaa 
     1321 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgtgattttc 
     1381 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac 
     1441 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt 
     1501 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg 
     1561 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata 
     1621 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga 
     1681 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt 
     1741 accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc 
     1801 gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac 
     1861 acgatgataa gcttgccaca acccgggatc caccggtcgc caccatggtg agcaagggcg 
     1921 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc 
     1981 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga 
     2041 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga 
     2101 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca 
     2161 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca 
     2221 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc 
     2281 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact 
     2341 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact 
     2401 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga 
     2461 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt 
     2521 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga 
     2581 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaaagcggc cctagagctc 
     2641 gctgatcagc ctcgactgtg cctctagttg ccagccatct gttgtttgcc cctcccccgt 
     2701 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat 
     2761 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag 
     2821 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc 
     2881 ttctgaggcg gaaagaacca gctggggctc gagtgcattc tagttgtggt ttgtccaaac 
     2941 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat 
     3001 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac 
     3061 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa 
     3121 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat 
     3181 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc 
     3241 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 
     3301 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 
     3361 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 
     3421 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 
     3481 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 
     3541 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 
     3601 aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 
     3661 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 
     3721 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 
     3781 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 
     3841 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 
     3901 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 
     3961 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 
     4021 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 
     4081 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 
     4141 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 
     4201 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 
     4261 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 
     4321 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 
     4381 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 
     4441 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 
     4501 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 
     4561 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 
     4621 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 
     4681 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 
     4741 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 
     4801 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 
     4861 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 
     4921 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 
     4981 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 
     5041 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 
     5101 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 
     5161 tc 
//

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