| 质粒名称: | pcDNA3.1-CMV-CFP;UBC-Cre25nt |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 65727 |
| 存储实验室: | Jacco van Rheenen |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 空载体大小: | 8225bp |
| 完整质粒大小: | 8228 bp |
| 载体类型: | 哺乳细胞表达、Cre/Lox |
| 筛选标记: | Zeocin |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | CMV promoter driving CFP expression |
| 启动子: | CMV |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | Mlul(未破坏) |
| 3’克隆位点: | EcoRI(未破坏) |
|
文献引用方法 |
|
| 材料和方法部分: | pcDNA3.1-CMV-CFP;UBC-Cre25nt was a gift from Jacco van Rheenen (Addgene plasmid # 65727 ; http://n2t.net/addgene:65727 ; RRID:Addgene_65727) |
| 参考文献部分: | In Vivo imaging reveals extracellular vesicle-mediated phenocopying of metastatic behavior. Zomer A, Maynard C, Verweij FJ, Kamermans A, Schafer R, Beerling E, Schiffelers RM, de Wit E, Berenguer J, Ellenbroek SI, Wurdinger T, Pegtel DM, van Rheenen J. Cell. 2015 May 21;161(5):1046-57. doi: 10.1016/j.cell.2015.04.042. 10.1016/j.cell.2015.04.042 PubMed 26000481 |
买家导航
pcDNA3.1-CMV-CFP;UBC-Cre25nt载体质粒基本信息
pcDNA3.1-CMV-CFP;UBC-Cre25nt质粒图谱载体图谱和pcDNA3.1-CMV-CFP;UBC-Cre25nt载体序列质粒序列多克隆位点信息
pcDNA3.1-CMV-CFP;UBC-Cre25nt质粒载体简介
pcDNA3.1-CMV-CFP;UBC-Cre25nt质粒序列载体序列
LOCUS pcDNA3.1-CMV-CFP;UBC-Cre25nt 8228 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Expresses Cre recombinase with a 25 nucleotide extracellular vesicle
targeting sequence under the UBC promotor and CFP under the CMV
promotor..
ACCESSION .
VERSION .
KEYWORDS pcDNA3.1-CMV-CFP;UBC-Cre25nt
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8228)
AUTHORS Zomer A, Maynard C, Verweij FJ, Kamermans A, Schafer R, Beerling E,
Schiffelers RM, de Wit E, Berenguer J, Ellenbroek SI, Wurdinger T,
Pegtel DM, van Rheenen J
TITLE In Vivo imaging reveals extracellular vesicle-mediated phenocopying
of metastatic behavior.
JOURNAL Cell. 2015 May 21;161(5):1046-57. doi: 10.1016/j.cell.2015.04.042.
PUBMED 26000481
REFERENCE 2 (bases 1 to 8228)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..8228
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 61..440
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 441..644
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 595..615
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 689..708
/label=T7
/note="T7 promoter, forward primer"
promoter 689..707
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
regulatory 730..739
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 736..1455
/codon_start=1
/product="enhanced CFP"
/label=ECFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(781..802)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(1042..1061)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 1389..1410
/label=EGFP-C
/note="EGFP, forward primer"
polyA_signal 1465..1689
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
promoter 1728..2939
/label=UbC promoter
/note="human ubiquitin C promoter"
primer_bind 2776..2795
/label=hUBCpro-F
/note="Human Ubiquitin C (UbC) promoter, forward primer"
CDS 2962..3993
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
primer_bind complement(4062..4079)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 4068..4292
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 4338..4766
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4425..4444)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4635..4656
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(4775..4795)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 4780..5109
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 4960..5095
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 5022..5041
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter 5157..5204
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 5223..5597
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 5727..5848
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5764..5783)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 5818..5837
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(5897..5913)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5897..5913)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(5910..5932)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 5921..5937
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5945..5975)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5990..6011
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(6128..6145)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6299..6887)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6379..6398)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7058..7918)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7681..7700
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7919..8023)
/gene="bla"
/label=AmpR promoter
primer_bind complement(8098..8117)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt
61 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
121 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
181 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
241 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
301 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
361 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
421 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
481 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
541 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
601 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
661 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
721 gctagctttg ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
781 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
841 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
901 gtgccctggc ccaccctcgt gaccaccctg acctggggcg tgcagtgctt cagccgctac
961 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
1021 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
1081 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
1141 aacatcctgg ggcacaagct ggagtacaac tacatcagcc acaacgtcta tatcaccgcc
1201 gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat cgaggacggc
1261 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
1321 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
1381 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
1441 gagctgtaca agtaaggatc ccgactgtgc cttctagttg ccagccatct gttgtttgcc
1501 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
1561 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
1621 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
1681 gctctatggg atctttttga attcgccctt ggccaaggca gccctggggc ctccgcgccg
1741 ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca cgtcagacga
1801 agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg cccgctgctc
1861 ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac gggacttggg
1921 tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa agtagtccct
1981 tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat tatataagga
2041 cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc ggttcttgtt
2101 tgtggatcgc tgtgatcgtc acttggtgag tagcgggctg ctgggctggc cggggctttc
2161 gtggccgccg ggccgctcgg tgggacggaa gcgtgtggag agaccgccaa gggctgtagt
2221 ctgggtccgc gagcaaggtt gccctgaact gggggttggg gggagcgcag caaaatggcg
2281 gctgttcccg agtcttgaat ggaagacgct tgtgaggcgg gctgtgaggt cgttgaaaca
2341 aggtgggggg catggtgggc ggcaagaacc caaggtcttg aggccttcgc taatgcggga
2401 aagctcttat tcgggtgaga tgggctgggg caccatctgg ggaccctgac gtgaagtttg
2461 tcactgactg gagaactcgg tttgtcgtct gttgcggggg cggcagttat ggcggtgccg
2521 ttgggcagtg cacccgtacc tttgggagcg cgcgccctcg tcgtgtcgtg acgtcacccg
2581 ttctgttggc ttataatgca gggtggggcc acctgccggt aggtgtgcgg taggcttttc
2641 tccgtcgcag gacgcagggt tcgggcctag ggtaggctct cctgaatcga caggcgccgg
2701 acctctggtg aggggaggga taagtgaggc gtcagtttct ttggtcggtt ttatgtacct
2761 atcttcttaa gtagctgaag ctccggtttt gaactatgcg ctcggggttg gcgagtgtgt
2821 tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc
2881 agtgttagac tagtaaattg tccgctaaat tctggccgtt tttggctttt ttgttagacg
2941 aagcttgggg tacccgccac catgtccaat ttactgaccg tacaccaaaa tttgcctgca
3001 ttaccggtcg atgcaacgag tgatgaggtt cgcaagaacc tgatggacat gttcagggat
3061 cgccaggcgt tttctgagca tacctggaaa atgcttctgt ccgtttgccg gtcgtgggcg
3121 gcatggtgca agttgaataa ccggaaatgg tttcccgcag aacctgaaga tgttcgcgat
3181 tatcttctat atcttcaggc gcgcggtctg gcagtaaaaa ctatccagca acatttgggc
3241 cagctaaaca tgcttcatcg tcggtccggg ctgccacgac caagtgacag caatgctgtt
3301 tcactggtta tgcggcggat ccgaaaagaa aacgttgatg ccggtgaacg tgcaaaacag
3361 gctctagcgt tcgaacgcac tgatttcgac caggttcgtt cactcatgga aaatagcgat
3421 cgctgccagg atatacgtaa tctggcattt ctggggattg cttataacac cctgttacgt
3481 atagccgaaa ttgccaggat cagggttaaa gatatctcac gtactgacgg tgggagaatg
3541 ttaatccata ttggcagaac gaaaacgctg gttagcaccg caggtgtaga gaaggcactt
3601 agcctggggg taactaaact ggtcgagcga tggatttccg tctctggtgt agctgatgat
3661 ccgaataact acctgttttg ccgggtcaga aaaaatggtg ttgccgcgcc atctgccacc
3721 agccagctat caactcgcgc cctggaaggg atttttgaag caactcatcg attgatttac
3781 ggcgctaagg atgactctgg tcagagatac ctggcctggt ctggacacag tgcccgtgtc
3841 ggagccgcgc gagatatggc ccgcgctgga gtttcaatac cggagatcat gcaagctggt
3901 ggctggacca atgtaaatat tgtcatgaac tatatccgta acctggatag tgaaacaggg
3961 gcaatggtgc gcctgctgga agatggcgat taggaatcca ccctgccgcc tggactccgc
4021 ctgtgaatcc tctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag
4081 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
4141 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
4201 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
4261 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
4321 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
4381 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
4441 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
4501 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
4561 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
4621 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
4681 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
4741 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga
4801 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
4861 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
4921 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
4981 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
5041 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
5101 ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgttg
5161 acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa
5221 ccatggccaa gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg
5281 tcgagttctg gaccgaccgg ctcgggttct cccgggactt cgtggaggac gacttcgccg
5341 gtgtggtccg ggacgacgtg accctgttca tcagcgcggt ccaggaccag gtggtgccgg
5401 acaacaccct ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg
5461 aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc catgaccgag atcggcgagc
5521 agccgtgggg gcgggagttc gccctgcgcg acccggccgg caactgcgtg cacttcgtgg
5581 ccgaggagca ggactgacac gtgctacgag atttcgattc caccgccgcc ttctatgaaa
5641 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
5701 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat
5761 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
5821 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga
5881 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
5941 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
6001 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
6061 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
6121 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
6181 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
6241 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
6301 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
6361 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
6421 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
6481 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
6541 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
6601 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
6661 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
6721 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
6781 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
6841 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
6901 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
6961 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
7021 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
7081 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
7141 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
7201 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
7261 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
7321 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
7381 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
7441 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
7501 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
7561 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
7621 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
7681 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
7741 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
7801 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
7861 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
7921 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
7981 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
8041 tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac
8101 aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt
8161 cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg
8221 catgaaga
//
