pLenti6.2_mCherry_roGFP2_Orp1

LOCUS       pLenti6.2_mCherry_roGFP2_Orp1        9982 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent redox reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9982)
  TITLE     Plasmids expressing redox reporters coupled to fluorescent protein 
            mCherry for lentiviral approaches
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti6.2_mCherry_roGFP2_Orp1
FEATURES             Location/Qualifiers
     source          1..9982
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(591..593,594..596,597..1307)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFISTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNCHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTCSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(636..657)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(897..916)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1244..1265
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1905..1958
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      1959..1968
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1971..1980
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1977..2687
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2122..2140)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2390..2409
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    2702..3290
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2755..2775)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3173..3184)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        3304..3803
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3339..3362)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3736..3755
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3824..4423
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3824..3843)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4320..4340
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4561..4794
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4866..4987
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4903..4922)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4957..4976
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5027..5162
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5182..5201)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5183..5201)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5211..5228)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5211..5227)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5220..5242)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5369..5824
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5456..5475)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5666..5687
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5850..5954
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5955..6023,6024..6815)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6173..6192)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6986..7574
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7475..7494
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7728..7745
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7862..7883
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7898..7903,7904..7921,7922..7928)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7936..7952
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7941..7963
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7960..7976
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7960..7976
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7995..8015
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7997..8015
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8043..8269
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8270..8450
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8497..8622
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9115..9348
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9533..9577
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9844..9960
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgactc tagagatatc atggtgagca
      601 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
      661 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
      721 ccctgaagtt catctccacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
      781 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
      841 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
      901 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
      961 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     1021 acaactacaa ctgccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     1081 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     1141 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     1201 cctgctccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     1261 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagact agtggtggtt
     1321 caggtggtgg tggttcaggt ggtggtggtt caggtggagg aggatcagga ggaggaggat
     1381 caggaggagg aggatcagga ggagaattcg atatctcaga attctataag ctagcacctg
     1441 ttgacaagaa aggccaacca ttccccttcg accaattaaa gggaaaagtg gtgcttatcg
     1501 ttaatgttgc ctccaaatgt ggattcactc ctcaatacaa agaactagag gccttgtaca
     1561 aacgttataa ggacgaagga tttaccatca tcgggttccc atgcaaccag tttggccacc
     1621 aagaacctgg ctctgatgaa gaaattgccc agttctgcca actgaactat ggcgtgactt
     1681 tccccattat gaaaaaaatt gacgttaatg gtggcaatga ggaccctgtt tacaagtttt
     1741 tgaagagcca aaaatccggt atgttgggct tgagaggtat caaatggaat tttgaaaaat
     1801 tcttagtcga taaaaagggt aaagtgtacg aaagatactc ttcactaacc aaaccttctt
     1861 cgttgtccga aaccatcgaa gaacttttga aagaggtgga aaccgagggc agaggaagtc
     1921 ttctaacatg cggtgacgtg gaggagaatc ccggccctcc atggtggcgc gccaccatgg
     1981 tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc
     2041 acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
     2101 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg
     2161 cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg
     2221 ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
     2281 tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg
     2341 agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc
     2401 agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc
     2461 tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg
     2521 tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca
     2581 tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg
     2641 ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtaggcg gccgcgtcga
     2701 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     2761 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     2821 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     2881 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     2941 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     3001 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     3061 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct
     3121 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     3181 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     3241 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattct
     3301 accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc
     3361 tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta
     3421 ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag
     3481 tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag
     3541 cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc
     3601 tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa
     3661 ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt
     3721 cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct
     3781 cttcctcatc tccgggcctt tcgacctgca gcccaagctt accatgaccg agtacaagcc
     3841 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
     3901 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
     3961 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     4021 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     4081 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     4141 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     4201 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     4261 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     4321 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     4381 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc
     4441 gcagcgcccg accgaaagga gcgcacgacc ccatgcatct cgagggcccg gtacctttaa
     4501 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     4561 tggaagggct agctcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     4621 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     4681 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     4741 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     4801 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     4861 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     4921 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     4981 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
     5041 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     5101 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     5161 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
     5221 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     5281 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     5341 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     5401 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     5461 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     5521 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     5581 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     5641 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     5701 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     5761 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     5821 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     5881 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     5941 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     6001 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     6061 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     6121 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     6181 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     6241 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     6301 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     6361 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     6421 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     6481 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     6541 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     6601 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     6661 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     6721 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     6781 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     6841 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     6901 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     6961 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     7021 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     7081 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     7141 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     7201 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     7261 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     7321 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     7381 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     7441 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     7501 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     7561 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     7621 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     7681 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     7741 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     7801 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     7861 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     7921 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     7981 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt
     8041 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
     8101 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
     8161 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
     8221 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
     8281 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     8341 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     8401 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     8461 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     8521 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     8581 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     8641 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     8701 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     8761 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     8821 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     8881 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     8941 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
     9001 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     9061 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     9121 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     9181 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     9241 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     9301 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     9361 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     9421 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     9481 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     9541 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     9601 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     9661 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     9721 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     9781 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     9841 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     9901 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     9961 atcgataagc ttgggagttc cg
//