| 质粒名称: | LSL-Cas9-Rosa26TV |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 61408 |
| 存储实验室: | Feng Zhang |
|
载体骨架基本信息 |
|
| 载体名称: | Ai9 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 14500 bp |
| 完整质粒大小: | 20541 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，小鼠靶向，Cre/Lox，CRISPR |
| 筛选标记: | 新霉素（G418）,DTA |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Cas9 |
| 物种来源: | Synthetic; streptococcus pyogenes |
| 插入大小: | 4101 bp |
| 基因突变: | human codon optimized |
| GenBank编号 | -- |
| 启动子 | CAG |
| 标签/融合蛋白: |
3xFLAG（插入件上的N端子）
P2A（主干上的C终端）
|
| 克隆方法: | Gibson克隆 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | atgtctggatccccatcaagc |
| 3' 测序引物 | CTGCTTGTCGGCCATGATATAG |
|
文献引用方法 |
|
| 材料和方法部分: | LSL-Cas9-Rosa26TV was a gift from Feng Zhang (Addgene plasmid # 61408 ; http://n2t.net/addgene:61408 ; RRID:Addgene_61408) |
| 参考文献部分: | CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling. Platt RJ, Chen S, Zhou Y, Yim MJ, Swiech L, Kempton HR, Dahlman JE, Parnas O, Eisenhaure TM, Jovanovic M, Graham DB, Jhunjhunwala S, Heidenreich M, Xavier RJ, Langer R, Anderson DG, Hacohen N, Regev A, Feng G, Sharp PA, Zhang F. Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 10.1016/j.cell.2014.09.014. 10.1016/j.cell.2014.09.014 PubMed 25263330 |
买家导航
LSL-Cas9-Rosa26TV载体质粒基本信息
LSL-Cas9-Rosa26TV质粒图谱载体图谱和LSL-Cas9-Rosa26TV载体序列质粒序列多克隆位点信息
LSL-Cas9-Rosa26TV质粒载体简介
LSL-Cas9-Rosa26TV质粒序列载体序列
LOCUS LSL-Cas9-Rosa26TV 20605 bp ds-DNA circular SYN 19-MAR-2020
DEFINITION Cre-dependent Cas9 expression plasmid and targeting vector for the
mouse Rosa26 locus. Cas9 expression is Cre-dependent and the
targeting vector has positive (Neo) and negative (DTA) selection..
ACCESSION .
VERSION .
KEYWORDS LSL-Cas9-Rosa26TV
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 20605)
AUTHORS Platt RJ, Chen S, Zhou Y, Yim MJ, Swiech L, Kempton HR, Dahlman JE,
Parnas O, Eisenhaure TM, Jovanovic M, Graham DB, Jhunjhunwala S,
Heidenreich M, Xavier RJ, Langer R, Anderson DG, Hacohen N, Regev A,
Feng G, Sharp PA, Zhang F
TITLE CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling.
JOURNAL Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi:
10.1016/j.cell.2014.09.014.
PUBMED 25263330
REFERENCE 2 (bases 1 to 20605)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Mar 19, 2020 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..20605
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 3..1085
/label=Rosa26 left arm
/note="left homology arm from intron 1 of the mouse Rosa26
gene (Soriano, 1999)"
enhancer 1181..1484
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 1486..1763
/label=chicken beta-actin promoter
intron 1764..2780
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
primer_bind 2704..2726
/label=pCAGGS-5
/note="Chimeric intron in CAG promoter, forward primer"
primer_bind 2788..2807
/label=pCAG-F
/note="Rabbit beta-globin intron, for pCAG plasmids,
forward primer"
protein_bind 2856..2889
/label=FRT (minimal)
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="supports FLP-mediated excision but not integration
(Turan and Bode, 2011)"
protein_bind complement(2913..2946)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
polyA_signal 3113..3247
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
polyA_signal 3367..3501
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
polyA_signal 3621..3755
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
protein_bind complement(3784..3817)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
regulatory 3875..3884
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 3884..3949
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
CDS 3956..3976
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 4001..8101
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS 8102..8149
/codon_start=1
/product="bipartite nuclear localization signal from
nucleoplasmin"
/label=nucleoplasmin NLS
/translation="KRPAATKKAGQAKKKK"
CDS 8159..8215
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS 8216..8932
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITLGMDELYK"
primer_bind complement(8258..8279)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(8519..8538)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 8866..8887
/label=EGFP-C
/note="EGFP, forward primer"
misc_feature 8983..9571
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(9036..9056)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(9454..9465)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(9574..9590)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(9575..9591)
/label=pBluescriptKS
/note="For pBluescript vector"
polyA_signal 9598..9805
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
protein_bind 9813..9846
/label=attB
/bound_moiety="phage phi-C31 integrase"
/note="minimal attB site for the phi-C31 integrase (Groth
et al., 2000)"
promoter 9868..10367
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
protein_bind 10387..10420
/label=FRT (minimal)
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="supports FLP-mediated excision but not integration
(Turan and Bode, 2011)"
regulatory 10421..10430
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 10427..11230
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(10490..10509)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 11100..11119
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 11240..11701
/label=PGK poly(A) signal
/note="mouse phosphoglycerate kinase 1 polyadenylation
signal"
misc_feature 11767..16107
/label=Rosa26 right arm
/note="right homology arm from intron 1 of the mouse Rosa26
gene (Soriano, 1999)"
promoter 16157..16656
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
CDS 16683..17255
/codon_start=1
/gene="Corynebacterium diphtheriae tox"
/product="diphtheria toxin fragment A"
/label=DTA
/translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
NFETRGKRGQDAMYEYMAQACAGNRVRR"
intron 17332..17397
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
polyA_signal 17471..17695
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(17757..17777)
/label=T3
/note="T3 promoter, forward primer"
promoter complement(17757..17775)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(17796..17812)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(17796..17812)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(17809..17831)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 17820..17836
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(17844..17874)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 17889..17910
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(18027..18044)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(18198..18786)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(18278..18297)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(18957..19817)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 19580..19599
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(19818..19922)
/gene="bla"
/label=AmpR promoter
rep_origin 19949..20404
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(20036..20055)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 20246..20267
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 20530..20552
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 20544..20561
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 20545..20561
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 20571..20590
/label=T7
/note="T7 promoter, forward primer"
promoter 20571..20589
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
1 ccgcggcagg ccctccgagc gtggtggagc cgttctgtga gacagccggg tacgagtcgt
61 gacgctggaa ggggcaagcg ggtggtgggc aggaatgcgg tccgccctgc agcaaccgga
121 gggggaggga gaagggagcg gaaaagtctc caccggacgc ggccatggct cggggggggg
181 ggggcagcgg aggagcgctt ccggccgacg tctcgtcgct gattggcttc ttttcctccc
241 gccgtgtgtg aaaacacaaa tggcgtgttt tggttggcgt aaggcgcctg tcagttaacg
301 gcagccggag tgcgcagccg ccggcagcct cgctctgccc actgggtggg gcgggaggta
361 ggtggggtga ggcgagctgg acgtgcgggc gcggtcggcc tctggcgggg cgggggaggg
421 gagggagggt cagcgaaagt agctcgcgcg cgagcggccg cccaccctcc ccttcctctg
481 ggggagtcgt tttacccgcc gccggccggg cctcgtcgtc tgattggctc tcggggccca
541 gaaaactggc ccttgccatt ggctcgtgtt cgtgcaagtt gagtccatcc gccggccagc
601 gggggcggcg aggaggcgct cccaggttcc ggccctcccc tcggccccgc gccgcagagt
661 ctggccgcgc gcccctgcgc aacgtggcag gaagcgcgcg ctgggggcgg ggacgggcag
721 tagggctgag cggctgcggg gcgggtgcaa gcacgtttcc gacttgagtt gcctcaagag
781 gggcgtgctg agccagacct ccatcgcgca ctccggggag tggagggaag gagcgagggc
841 tcagttgggc tgttttggag gcaggaagca cttgctctcc caaagtcgct ctgagttgtt
901 atcagtaagg gagctgcagt ggagtaggcg gggagaaggc cgcacccttc tccggagggg
961 ggaggggagt gttgcaatac ctttctggga gttctctgct gcctcctggc ttctgaggac
1021 cgccctgggc ctgggagaat cccttccccc tcttccctcg tgatctgcaa ctccagtctt
1081 tctagcctta attaaccgtt taaacaattc tgcaggaatc tagttattaa tagtaatcaa
1141 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
1201 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
1261 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
1321 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
1381 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
1441 ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca
1501 cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg tatttattta
1561 ttttttaatt attttgtgca gcgatggggg cggggggggg gggggggcgc gcgccaggcg
1621 gggcggggcg gggcgagggg cggggcgggg cgaggcggag aggtgcggcg gcagccaatc
1681 agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata
1741 aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct
1801 ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga
1861 gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg
1921 tttcttttct gtggctgcgt gaaagccttg aggggctccg ggagggccct ttgtgcgggg
1981 ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg
2041 cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg
2101 tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg cggggggggc tgcgagggga
2161 acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg
2221 tcgggctgca accccccctg cacccccctc cccgagttgc tgagcacggc ccggcttcgg
2281 gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc
2341 aggtgggggt gccgggcggg gcggggccgc ctcgggccgg ggagggctcg ggggaggggc
2401 gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc cattgccttt
2461 tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgt gcggagccga
2521 aatctgggag gcgccgccgc accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg
2581 gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc
2641 ctctccagcc tcggggctgt ccgcgggggg acggctgcct tcggggggga cggggcaggg
2701 cggggttcgg cttctggcgt gtgaccggcg gctctagagc ctctgctaac catgttcatg
2761 ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg tctcatcatt
2821 ttggcaaaga attgatttga taccgcgggc cctaagaagt tcctattctc tagaaagtat
2881 aggaacttcg tcgacattta aatcatttaa atataacttc gtataatgta tgctatacga
2941 agttattcgc gatgaataaa tgaaagcttg cagatctgcg actctagagg atctgcgact
3001 ctagaggatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
3061 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
3121 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
3181 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
3241 ctggatctgc gactctagag gatcataatc agccatacca catttgtaga ggttttactt
3301 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
3361 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
3421 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
3481 gtatcttatc atgtctggat ctgcgactct agaggatcat aatcagccat accacatttg
3541 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
3601 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
3661 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
3721 ccaaactcat caatgtatct tatcatgtct ggatccccat caagctgatc cggaaccctt
3781 aatataactt cgtataatgt atgctatacg aagttattag gtccctcgac ctgcagccca
3841 agctagatcg aattcggccg gccttcgaac acgtgccacc atggactata aggaccacga
3901 cggagactac aaggatcatg atattgatta caaagacgat gacgataaga tggccccaaa
3961 gaagaagcgg aaggtcggta tccacggagt cccagcagcc gacaagaagt acagcatcgg
4021 cctggacatc ggcaccaact ctgtgggctg ggccgtgatc accgacgagt acaaggtgcc
4081 cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac agcatcaaga agaacctgat
4141 cggagccctg ctgttcgaca gcggcgaaac agccgaggcc acccggctga agagaaccgc
4201 cagaagaaga tacaccagac ggaagaaccg gatctgctat ctgcaagaga tcttcagcaa
4261 cgagatggcc aaggtggacg acagcttctt ccacagactg gaagagtcct tcctggtgga
4321 agaggataag aagcacgagc ggcaccccat cttcggcaac atcgtggacg aggtggccta
4381 ccacgagaag taccccacca tctaccacct gagaaagaaa ctggtggaca gcaccgacaa
4441 ggccgacctg cggctgatct atctggccct ggcccacatg atcaagttcc ggggccactt
4501 cctgatcgag ggcgacctga accccgacaa cagcgacgtg gacaagctgt tcatccagct
4561 ggtgcagacc tacaaccagc tgttcgagga aaaccccatc aacgccagcg gcgtggacgc
4621 caaggccatc ctgtctgcca gactgagcaa gagcagacgg ctggaaaatc tgatcgccca
4681 gctgcccggc gagaagaaga atggcctgtt cggaaacctg attgccctga gcctgggcct
4741 gacccccaac ttcaagagca acttcgacct ggccgaggat gccaaactgc agctgagcaa
4801 ggacacctac gacgacgacc tggacaacct gctggcccag atcggcgacc agtacgccga
4861 cctgtttctg gccgccaaga acctgtccga cgccatcctg ctgagcgaca tcctgagagt
4921 gaacaccgag atcaccaagg cccccctgag cgcctctatg atcaagagat acgacgagca
4981 ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag cagctgcctg agaagtacaa
5041 agagattttc ttcgaccaga gcaagaacgg ctacgccggc tacattgacg gcggagccag
5101 ccaggaagag ttctacaagt tcatcaagcc catcctggaa aagatggacg gcaccgagga
5161 actgctcgtg aagctgaaca gagaggacct gctgcggaag cagcggacct tcgacaacgg
5221 cagcatcccc caccagatcc acctgggaga gctgcacgcc attctgcggc ggcaggaaga
5281 tttttaccca ttcctgaagg acaaccggga aaagatcgag aagatcctga ccttccgcat
5341 cccctactac gtgggccctc tggccagggg aaacagcaga ttcgcctgga tgaccagaaa
5401 gagcgaggaa accatcaccc cctggaactt cgaggaagtg gtggacaagg gcgcttccgc
5461 ccagagcttc atcgagcgga tgaccaactt cgataagaac ctgcccaacg agaaggtgct
5521 gcccaagcac agcctgctgt acgagtactt caccgtgtat aacgagctga ccaaagtgaa
5581 atacgtgacc gagggaatga gaaagcccgc cttcctgagc ggcgagcaga aaaaggccat
5641 cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg aagcagctga aagaggacta
5701 cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc ggcgtggaag atcggttcaa
5761 cgcctccctg ggcacatacc acgatctgct gaaaattatc aaggacaagg acttcctgga
5821 caatgaggaa aacgaggaca ttctggaaga tatcgtgctg accctgacac tgtttgagga
5881 cagagagatg atcgaggaac ggctgaaaac ctatgcccac ctgttcgacg acaaagtgat
5941 gaagcagctg aagcggcgga gatacaccgg ctggggcagg ctgagccgga agctgatcaa
6001 cggcatccgg gacaagcagt ccggcaagac aatcctggat ttcctgaagt ccgacggctt
6061 cgccaacaga aacttcatgc agctgatcca cgacgacagc ctgaccttta aagaggacat
6121 ccagaaagcc caggtgtccg gccagggcga tagcctgcac gagcacattg ccaatctggc
6181 cggcagcccc gccattaaga agggcatcct gcagacagtg aaggtggtgg acgagctcgt
6241 gaaagtgatg ggccggcaca agcccgagaa catcgtgatc gaaatggcca gagagaacca
6301 gaccacccag aagggacaga agaacagccg cgagagaatg aagcggatcg aagagggcat
6361 caaagagctg ggcagccaga tcctgaaaga acaccccgtg gaaaacaccc agctgcagaa
6421 cgagaagctg tacctgtact acctgcagaa tgggcgggat atgtacgtgg accaggaact
6481 ggacatcaac cggctgtccg actacgatgt ggaccatatc gtgcctcaga gctttctgaa
6541 ggacgactcc atcgacaaca aggtgctgac cagaagcgac aagaaccggg gcaagagcga
6601 caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac tactggcggc agctgctgaa
6661 cgccaagctg attacccaga gaaagttcga caatctgacc aaggccgaga gaggcggcct
6721 gagcgaactg gataaggccg gcttcatcaa gagacagctg gtggaaaccc ggcagatcac
6781 aaagcacgtg gcacagatcc tggactcccg gatgaacact aagtacgacg agaatgacaa
6841 gctgatccgg gaagtgaaag tgatcaccct gaagtccaag ctggtgtccg atttccggaa
6901 ggatttccag ttttacaaag tgcgcgagat caacaactac caccacgccc acgacgccta
6961 cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac cctaagctgg aaagcgagtt
7021 cgtgtacggc gactacaagg tgtacgacgt gcggaagatg atcgccaaga gcgagcagga
7081 aatcggcaag gctaccgcca agtacttctt ctacagcaac atcatgaact ttttcaagac
7141 cgagattacc ctggccaacg gcgagatccg gaagcggcct ctgatcgaga caaacggcga
7201 aaccggggag atcgtgtggg ataagggccg ggattttgcc accgtgcgga aagtgctgag
7261 catgccccaa gtgaatatcg tgaaaaagac cgaggtgcag acaggcggct tcagcaaaga
7321 gtctatcctg cccaagagga acagcgataa gctgatcgcc agaaagaagg actgggaccc
7381 taagaagtac ggcggcttcg acagccccac cgtggcctat tctgtgctgg tggtggccaa
7441 agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa gagctgctgg ggatcaccat
7501 catggaaaga agcagcttcg agaagaatcc catcgacttt ctggaagcca agggctacaa
7561 agaagtgaaa aaggacctga tcatcaagct gcctaagtac tccctgttcg agctggaaaa
7621 cggccggaag agaatgctgg cctctgccgg cgaactgcag aagggaaacg aactggccct
7681 gccctccaaa tatgtgaact tcctgtacct ggccagccac tatgagaagc tgaagggctc
7741 ccccgaggat aatgagcaga aacagctgtt tgtggaacag cacaagcact acctggacga
7801 gatcatcgag cagatcagcg agttctccaa gagagtgatc ctggccgacg ctaatctgga
7861 caaagtgctg tccgcctaca acaagcaccg ggataagccc atcagagagc aggccgagaa
7921 tatcatccac ctgtttaccc tgaccaatct gggagcccct gccgccttca agtactttga
7981 caccaccatc gaccggaaga ggtacaccag caccaaagag gtgctggacg ccaccctgat
8041 ccaccagagc atcaccggcc tgtacgagac acggatcgac ctgtctcagc tgggaggcga
8101 caaaaggccg gcggccacga aaaaggccgg ccaggcaaaa aagaaaaagg gaagcggagc
8161 cactaacttc tccctgttga aacaagcagg ggatgtcgaa gagaatcccg ggccagtgag
8221 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
8281 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
8341 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
8401 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
8461 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
8521 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
8581 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
8641 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
8701 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
8761 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
8821 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
8881 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt aaacgcgtat
8941 gcatggccgg ccctgcagga attcgatatc aagcttatcg ataatcaacc tctggattac
9001 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
9061 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
9121 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
9181 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
9241 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
9301 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
9361 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
9421 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
9481 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
9541 agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg acctcgactg
9601 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
9661 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
9721 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
9781 aagacaatgg caggcatgct ggggaactag tggtgccagg gcgtgccctt gggctccccg
9841 ggcgcggcgg ccgcatcgaa ttctaccggg taggggaggc gcttttccca aggcagtctg
9901 gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc
9961 gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg
10021 cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt
10081 gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg
10141 ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg
10201 ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca
10261 ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc
10321 gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcaatcgcc
10381 gctagcgaag ttcctattct ctagaaagta taggaacttc gccaccatgg gatcggccat
10441 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
10501 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
10561 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
10621 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
10681 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
10741 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
10801 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
10861 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
10921 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
10981 tgatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
11041 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
11101 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
11161 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
11221 gttcttctga ggggatccgc tgtaagtctg cagaaattga tgatctatta aacaataaag
11281 atgtccacta aaatggaagt ttttcctgtc atactttgtt aagaagggtg agaacagagt
11341 acctacattt tgaatggaag gattggagct acgggggtgg gggtggggtg ggattagata
11401 aatgcctgct ctttactgaa ggctctttac tattgcttta tgataatgtt tcatagttgg
11461 atatcataat ttaaacaagc aaaaccaaat taagggccag ctcattcctc ccactcatga
11521 tctatagatc tatagatctc tcgtgggatc attgtttttc tcttgattcc cactttgtgg
11581 ttctaagtac tgtggtttcc aaatgtgtca gtttcatagc ctgaagaacg agatcagcag
11641 cctctgttcc acatacactt cattctcagt attgttttgc caagttctaa ttccatcaga
11701 aagcttgcag aagatctccc caactggggt aacctttgag ttctctcagt tgggggggcg
11761 cgccggctag aagatgggcg ggagtcttct gggcaggctt aaaggctaac ctggtgtgtg
11821 ggcgttgtcc tgcaggggaa ttgaacaggt gtaaaattgg agggacaaga cttcccacag
11881 attttcggtt ttgtcgggaa gttttttaat aggggcaaat aaggaaaatg ggaggatagg
11941 tagtcatctg gggttttatg cagcaaaact acaggttatt attgcttgtg atccgcctcg
12001 gagtattttc catcgaggta gattaaagac atgctcaccc gagttttata ctctcctgct
12061 tgagatcctt actacagtat gaaattacag tgtcgcgagt tagactatgt aagcagaatt
12121 ttaatcattt ttaaagagcc cagtacttca tatccatttc tcccgctcct tctgcagcct
12181 tatcaaaagg tattttagaa cactcatttt agccccattt tcatttatta tactggctta
12241 tccaacccct agacagagca ttggcatttt ccctttcctg atcttagaag tctgatgact
12301 catgaaacca gacagattag ttacatacac cacaaatcga ggctgtagct ggggcctcaa
12361 cactgcagtt cttttataac tccttagtac actttttgtt gatcctttgc cttgatcctt
12421 aattttcagt gtctatcacc tctcccgtca ggtggtgttc cacatttggg cctattctca
12481 gtccagggag ttttacaaca atagatgtat tgagaatcca acctaaagct taactttcca
12541 ctcccatgaa tgcctctctc ctttttctcc atttataaac tgagctatta accattaatg
12601 gtttccaggt ggatgtctcc tcccccaata ttacctgatg tatcttacat attgccaggc
12661 tgatatttta agacattaaa aggtatattt cattattgag ccacatggta ttgattactg
12721 cttactaaaa ttttgtcatt gtacacatct gtaaaaggtg gttccttttg gaatgcaaag
12781 ttcaggtgtt tgttgtcttt cctgacctaa ggtcttgtga gcttgtattt tttctattta
12841 agcagtgctt tctcttggac tggcttgact catggcattc tacacgttat tgctggtcta
12901 aatgtgattt tgccaagctt cttcaggacc tataattttg cttgacttgt agccaaacac
12961 aagtaaaatg attaagcaac aaatgtattt gtgaagcttg gtttttaggt tgttgtgttg
13021 tgtgtgcttg tgctctataa taatactatc caggggctgg agaggtggct cggagttcaa
13081 gagcacagac tgctcttcca gaagtcctga gttcaattcc cagcaaccac atggtggctc
13141 acaaccatct gtaatgggat ctgatgccct cttctggtgt gtctgaagac cacaagtgta
13201 ttcacattaa ataaataaat cctccttctt cttctttttt ttttttttaa agagaatact
13261 gtctccagta gaatttactg aagtaatgaa atactttgtg tttgttccaa tatggtagcc
13321 aataatcaaa ttactcttta agcactggaa atgttaccaa ggaactaatt tttatttgaa
13381 gtgtaactgt ggacagagga gccataactg cagacttgtg ggatacagaa gaccaatgca
13441 gactttaatg tcttttctct tacactaagc aataaagaaa taaaaattga acttctagta
13501 tcctatttgt ttaaactgct agctttactt aacttttgtg cttcatctat acaaagctga
13561 aagctaagtc tgcagccatt actaaacatg aaagcaagta atgataattt tggatttcaa
13621 aaatgtaggg ccagagttta gccagccagt ggtggtgctt gcctttatgc ctttaatccc
13681 agcactctgg aggcagagac aggcagatct ctgagtttga gcccagcctg gtctacacat
13741 caagttctat ctaggatagc caggaataca cacagaaacc ctgttgggga ggggggctct
13801 gagatttcat aaaattataa ttgaagcatt ccctaatgag ccactatgga tgtggctaaa
13861 tccgtctacc tttctgatga gatttgggta ttattttttc tgtctctgct gttggttggg
13921 tcttttgaca ctgtgggctt tctttaaagc ctccttcctg ccatgtggtc tcttgtttgc
13981 tactaacttc ccatggctta aatggcatgg ctttttgcct tctaagggca gctgctgaga
14041 tttgcagcct gatttccagg gtggggttgg gaaatctttc aaacactaaa attgtccttt
14101 aatttttttt ttaaaaaatg ggttatataa taaacctcat aaaatagtta tgaggagtga
14161 ggtggactaa tattaaatga gtccctcccc tataaaagag ctattaaggc tttttgtctt
14221 atacttaact ttttttttaa atgtggtatc tttagaacca agggtcttag agttttagta
14281 tacagaaact gttgcatcgc ttaatcagat tttctagttt caaatccaga gaatccaaat
14341 tcttcacagc caaagtcaaa ttaagaattt ctgactttta atgttaattt gcttactgtg
14401 aatataaaaa tgatagcttt tcctgaggca gggtctcact atgtatctct gcctgatctg
14461 caacaagata tgtagactaa agttctgcct gcttttgtct cctgaatact aaggttaaaa
14521 tgtagtaata cttttggaac ttgcaggtca gattctttta taggggacac actaagggag
14581 cttgggtgat agttggtaaa atgtgtttca agtgatgaaa acttgaatta ttatcaccgc
14641 aacctacttt ttaaaaaaaa aagccaggcc tgttagagca tgcttaaggg atccctagga
14701 cttgctgagc acacaagagt agttacttgg caggctcctg gtgagagcat atttcaaaaa
14761 acaaggcaga caaccaagaa actacagtta aggttacctg tctttaaacc atctgcatat
14821 acacagggat attaaaatat tccaaataat atttcattca agttttcccc catcaaattg
14881 ggacatggat ttctccggtg aataggcaga gttggaaact aaacaaatgt tggttttgtg
14941 atttgtgaaa ttgttttcaa gtgatagtta aagcccatga gatacagaac aaagctgcta
15001 tttcgaggtc tcttggttta tactcagaag cacttctttg ggtttccctg cactatcctg
15061 atcatgtgct aggcctacct taggctgatt gttgttcaaa taaacttaag tttcctgtca
15121 ggtgatgtca tatgatttca tatatcaagg caaaacatgt tatatatgtt aaacatttgt
15181 acttaatgtg aaagttaggt ctttgtgggt ttgattttta attttcaaaa cctgagctaa
15241 ataagtcatt tttacatgtc ttacatttgg tggaattgta taattgtggt ttgcaggcaa
15301 gactctctga cctagtaacc ctacctatag agcactttgc tgggtcacaa gtctaggagt
15361 caagcatttc accttgaagt tgagacgttt tgttagtgta tactagttta tatgttggag
15421 gacatgttta tccagaagat attcaggact atttttgact gggctaagga attgattctg
15481 attagcactg ttagtgagca ttgagtggcc tttaggcttg aattggagtc acttgtatat
15541 ctcaaataat gctggccttt tttaaaaagc ccttgttctt tatcaccctg ttttctacat
15601 aatttttgtt caaagaaata cttgtttgga tctccttttg acaacaatag catgttttca
15661 agccatattt tttttccttt tttttttttt ttttggtttt tcgagacagg gtttctctgt
15721 atagccctgg ctgtcctgga actcactttg tagaccaggc tggcctcgaa ctcagaaatc
15781 cgcctgcctc tgcctcctga gtgccgggat taaaggcgtg caccaccacg cctggctaag
15841 ttggatattt tgttatataa ctataaccaa tactaactcc actgggtgga tttttaattc
15901 agtcagtagt cttaagtggt ctttattggc ccttcattaa aatctactgt tcactctaac
15961 agaggctgtt ggtactagtg gcacttaagc aacttcctac ggatatacta gcagattaag
16021 ggtcagggat agaaactagt ctagcgtttt gtatacctac cagctttata ctaccttgtt
16081 ctgatagaaa tatttcagga catctagctt atcgataccg tcgacggtat cgataagctt
16141 gatatcgaat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct
16201 ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc
16261 acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct
16321 actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga
16381 caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg
16441 aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct
16501 cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg
16561 ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc
16621 gcgctgttct cctcttcctc atctccgggc ctttcgacct gcaggtcctc gccatggatc
16681 ctgatgatgt tgttgattct tctaaatctt ttgtgatgga aaacttttct tcgtaccacg
16741 ggactaaacc tggttatgta gattccattc aaaaaggtat acaaaagcca aaatctggta
16801 cacaaggaaa ttatgacgat gattggaaag ggttttatag taccgacaat aaatacgacg
16861 ctgcgggata ctctgtagat aatgaaaacc cgctctctgg aaaagctgga ggcgtggtca
16921 aagtgacgta tccaggactg acgaaggttc tcgcactaaa agtggataat gccgaaacta
16981 ttaagaaaga gttaggttta agtctcactg aaccgttgat ggagcaagtc ggaacggaag
17041 agtttatcaa aaggttcggt gatggtgctt cgcgtgtagt gctcagcctt cccttcgctg
17101 aggggagttc tagcgttgaa tatattaata actgggaaca ggcgaaagcg ttaagcgtag
17161 aacttgagat taattttgaa acccgtggaa aacgtggcca agatgcgatg tatgagtata
17221 tggctcaagc ctgtgcagga aatcgtgtca ggcgatctct ttgtgaagga accttacttc
17281 tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa ggtaaatata
17341 aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt
17401 ccaacctatg gaactgatga atgggagcag tggtggaatg cagatcctag agctcgctga
17461 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
17521 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
17581 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
17641 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
17701 gaggcggaaa gaaccagctg gggctcgagg gggggcccgg tacccagctt ttgttccctt
17761 tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
17821 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
17881 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
17941 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
18001 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
18061 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
18121 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
18181 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
18241 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
18301 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
18361 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
18421 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
18481 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
18541 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
18601 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
18661 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
18721 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
18781 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
18841 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
18901 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
18961 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
19021 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
19081 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
19141 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
19201 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
19261 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
19321 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
19381 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
19441 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
19501 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
19561 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
19621 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
19681 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
19741 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
19801 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
19861 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
19921 cgcacatttc cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg
19981 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
20041 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
20101 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
20161 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
20221 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
20281 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
20341 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
20401 atttccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
20461 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
20521 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact
20581 cactataggg cgaattggag ctcca
//
