| 质粒名称: | pTD103aiiA(Cm) |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 48886 |
| 存储实验室: | Jeff Hasty |
|
载体骨架基本信息 |
|
| 载体名称: | pZ vector |
| 载体出品公司: | -- |
| 载体抗性: | 氯霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 细菌表达，合成生物学 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | aiiA and LuxR |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | pLux |
| 标签/融合蛋白: | ssrA标签（AANDENYALAA）（C端子插入） |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pTD103aiiA(Cm) was a gift from Jeff Hasty (Addgene plasmid # 48886 ; http://n2t.net/addgene:48886 ; RRID:Addgene_48886) |
| 参考文献部分: | A sensing array of radically coupled genetic 'biopixels'. Prindle A, Samayoa P, Razinkov I, Danino T, Tsimring LS, Hasty J. Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722. 10.1038/nature10722 PubMed 22178928 |
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
质粒图谱.png)
载体简介
载体序列
LOCUS pTD103aiiA(Cm) 3678 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Negative feedback plasmid from oscillator.
ACCESSION .
VERSION .
KEYWORDS pTD103aiiA(Cm)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3678)
AUTHORS Prindle A, Samayoa P, Razinkov I, Danino T, Tsimring LS, Hasty J
TITLE A sensing array of radically coupled genetic 'biopixels'.
JOURNAL Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722.
PUBMED 22178928
REFERENCE 2 (bases 1 to 3678)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..3678
/organism="synthetic DNA construct"
/mol_type="other DNA"
RBS 968..979
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
terminator 1789..1875
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
primer_bind complement(1910..1927)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(2044..2589)
/direction=LEFT
/label=p15A ori
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin.
"
primer_bind complement(2124..2143)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
terminator 2703..2797
/label=lambda t0 terminator
/note="transcription terminator from phage lambda"
CDS complement(2818..3477)
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
primer_bind 3392..3411
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
promoter complement(3478..3580)
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
primer_bind 3646..3664
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
ORIGIN
1 ctcgagttaa tttttaaagt atgggcaatc aattgctcct gttaaaattg ctttagaaat
61 actttggcag cggtttgttg tattgagttt catttgcgca ttggttaaat ggaaagtgac
121 agtacgctca ctgcagccta atatttttga aatatcccaa gagctttttc cttcgcatgc
181 ccacgctaaa cattcttttt ctcttttggt taaatcgttg tttgatttat tatttgctat
241 atttattttt cgataattat caactagaga aggaacaatt aatggtatgt tcatacacgc
301 atgtaaaaat aaactatcta tatagttgtc tttttctgaa tgtgcaaaac taagcattcc
361 gaagccattg ttagccgtat gaatagggaa actaaaccca gtgataagac ctgatgtttt
421 cgcttcttta attacatttg gagatttttt atttacagca ttgttttcaa atatattcca
481 attaattggt gaatgattgg agttagaata atctactata ggatcatatt ttattaaatt
541 agcgtcatca taatattgcc tccatttttt agggtaatta tctagaattg aaatatcaga
601 tttaaccata gaatgaggat aaatgatcgc gagtaaataa tattcacaat gtaccatttt
661 agtcatatca gataagcatt gattaatatc attattgctt ctacaagctt taattttatt
721 aattattctg tatgtgtcgt cggcatttat gtttttcata cccatctctt tatccttacc
781 tattgtttgt cgcaagtttt gcgtgttata tatcattaaa acggtaatgg attgacattt
841 gattctaata aattggattt ttgtcacact attgtatcgc tgggaataca attacttaac
901 ataagcacct gtaggatcgt acaggtttac gcaagaaaat ggtttgttat agtcgaatga
961 attcattaaa gaggagaaag gtaccatgac agtaaagaaa ctttatttca tcccagcagg
1021 tcgttgcatg ttggatcatt cgtctgttaa cagtgcgtta acaccgggga aactattaaa
1081 cttgccggtg tggtgttatc ttttggagac ggaagaaggt cctattttag tagacacagg
1141 tatgccagaa agtgcagtta ataatgaagg gctttttaac ggtacatttg ttgaaggaca
1201 gatcttaccg aaaatgactg aagaagatag aatcgtgaat atattaaagc gtgtagggta
1261 tgagccggac gaccttttat atattattag ttctcactta cattttgatc atgcaggagg
1321 aaacggtgct tttacaaata caccaattat tgtgcagcga acggaatatg aggcagcact
1381 tcatagagaa gaatatatga aagaatgtat attaccgcat ttgaactaca aaattattga
1441 aggggattat gaagtggtac caggtgttca attattgtat acgccaggtc attctccagg
1501 ccatcagtcg ctattcattg agacggagca atccggttca gttttattaa cgattgatgc
1561 atcgtacacg aaagagaatt ttgaagatga agtgccgttc gcaggatttg atccagaatt
1621 agctttatct tcaattaaac gtttaaaaga agttgtgaaa aaagagaaac caattatttt
1681 ctttggtcat gatatagagc aggaaaagag ttgtagagtg ttcccggaat atatagcagc
1741 gaacgacgaa aattacgccc ttgcagcgta aacgcgtgct agaggcatca aataaaacga
1801 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
1861 ctgagtagga caaatccgcc gccctagacc tagggatata ttccgcttcc tcgctcactg
1921 actcgctacg ctcggtcgtt cgactgcggc gagcggaaat ggcttacgaa cggggcggag
1981 atttcctgga agatgccagg aagatactta acagggaagt gagagggccg cggcaaagcc
2041 gtttttccat aggctccgcc cccctgacaa gcatcacgaa atctgacgct caaatcagtg
2101 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggcg gctccctcgt
2161 gcgctctcct gttcctgcct ttcggtttac cggtgtcatt ccgctgttat ggccgcgttt
2221 gtctcattcc acgcctgaca ctcagttccg ggtaggcagt tcgctccaag ctggactgta
2281 tgcacgaacc ccccgttcag tccgaccgct gcgccttatc cggtaactat cgtcttgagt
2341 ccaacccgga aagacatgca aaagcaccac tggcagcagc cactggtaat tgatttagag
2401 gagttagtct tgaagtcatg cgccggttaa ggctaaactg aaaggacaag ttttggtgac
2461 tgcgctcctc caagccagtt acctcggttc aaagagttgg tagctcagag aaccttcgaa
2521 aaaccgccct gcaaggcggt tttttcgttt tcagagcaag agattacgcg cagaccaaaa
2581 cgatctcaag aagatcatct tattaatcag ataaaatatt tctagatttc agtgcaattt
2641 atctcttcaa atgtagcacc tgaagtcagc cccatacgat ataagttgtt actagtgctt
2701 ggattctcac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg
2761 agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctcga tatcaaatta
2821 cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg
2881 gaagccatca cagacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc
2941 ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac
3001 gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa acatattctc
3061 aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata
3121 tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc
3181 agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc
3241 gtctttcatt gccatacgga attccggatg agcattcatc aggcgggcaa gaatgtgaat
3301 aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc
3361 cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc
3421 tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt
3481 agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat
3541 ttcattatgg tgaaagttgg aacctcttac gtgccgatca acgtctcatt ttcgccagat
3601 atcgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
3661 aggccctttc gtcttcac
//
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