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载体序列
LOCUS p3xFLAG-Myc-CMV-24 4752 bp ds-DNA circular SYN 23-OCT-2018
DEFINITION Vector for expression of 3xFLAG- and Myc-tagged proteins in
mammalian cells.
ACCESSION .
VERSION .
KEYWORDS p3xFLAG-Myc-CMV-24
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4752)
AUTHORS MilliporeSigma
TITLE Direct Submission
JOURNAL Exported Saturday, December 21, 2019 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..4752
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
enhancer 318..697
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 698..901
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 928..930
/codon_start=1
/product="start codon"
/note="ATG"
/translation="M"
CDS 931..996
/codon_start=1
/product="three tandem FLAG epitope tags, followed by an
enterokinase cleavage site"
/note="3xFLAG"
/translation="DYKDHDGDYKDHDIDYKDDDDK"
misc_feature 994..1050
/note="MCS"
/note="multiple cloning site"
CDS 1057..1086
/codon_start=1
/product="Myc (human c-Myc oncogene) epitope tag"
/note="Myc"
/translation="EQKLISEEDL"
polyA_signal 1093..1715
/note="hGH poly(A) signal"
/note="human growth hormone polyadenylation signal"
rep_origin 1924..2059
/note="SV40 ori"
/note="SV40 origin of replication"
rep_origin complement(2546..3134)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3305..4165)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(4166..4270)
/gene="bla"
/note="AmpR promoter"
rep_origin 4297..4752
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
ORIGIN
1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
61 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
121 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa
181 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta
241 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
301 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg
361 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
421 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
481 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
541 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
601 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
661 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
721 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
781 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
841 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
901 tcgtttagtg aaccgtcaga attaaccatg gactacaaag accatgacgg tgattataaa
961 gatcatgaca tcgattacaa ggatgacgat gacaagcttg cggccgcgaa ttcatcgata
1021 gatctgatat cggtaccagt cgactctaga ggatccgaac aaaaactcat ctcagaagag
1081 gatctgtgac ccgggtggca tccctgtgac ccctccccag tgcctctcct ggccctggaa
1141 gttgccactc cagtgcccac cagccttgtc ctaataaaat taagttgcat cattttgtct
1201 gactaggtgt ccttctataa tattatgggg tggagggggg tggtatggag caaggggcaa
1261 gttgggaaga caacctgtag ggcctgcggg gtctattggg aaccaagctg gagtgcagtg
1321 gcacaatctt ggctcactgc aatctccgcc tcctgggttc aagcgattct cctgcctcag
1381 cctcccgagt tgttgggatt ccaggcatgc atgaccaggc tcagctaatt tttgtttttt
1441 tggtagagac ggggtttcac catattggcc aggctggtct ccaactccta atctcaggtg
1501 atctacccac cttggcctcc caaattgctg ggattacagg cgtgaaccac tgctcccttc
1561 cctgtccttc tgattttaaa ataactatac cagcaggagg acgtccagac acagcatagg
1621 ctacctggcc atgcccaacc ggtgggacat ttgagttgct tgcttggcac tgtcctctca
1681 tgcgttgggt ccactcagta gatgcctgtt gaattgggta cgcggccagc ttggctgtgg
1741 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
1801 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
1861 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
1921 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
1981 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
2041 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc tcgaggaact gaaaaaccag
2101 aaagttaatt ccctatagtg agtcgtatta aattcgtaat catgtcatag ctgtttcctg
2161 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
2221 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
2281 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
2341 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
2401 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
2461 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
2521 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
2581 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
2641 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
2701 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
2761 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
2821 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
2881 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
2941 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
3001 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
3061 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
3121 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
3181 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
3241 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
3301 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
3361 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
3421 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
3481 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
3541 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
3601 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
3661 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
3721 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
3781 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
3841 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
3901 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
3961 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
4021 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
4081 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
4141 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
4201 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
4261 gggttccgcg cacatttccc cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa
4321 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
4381 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
4441 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
4501 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
4561 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
4621 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
4681 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
4741 cgcttacaat tt
//
