pLVX-PAmCherry-C1

价格:2000元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Clontech
载体名称: pLVX-PAmCherry-C1
质粒类型: 慢病毒载体;哺乳动物表达载体;荧光报告载体
高拷贝/低拷贝: 高拷贝
启动子: CMV
克隆方法: 多克隆位点,限制性内切酶
载体大小: 8770 bp
5' 测序引物及序列: CMV-F: CGCAAATGGGCGGTAGGCGTG (Invitrogen)
3' 测序引物及序列: --
载体标签: PAmCherry(N-端)
载体抗性: 氨苄青霉素
筛选标记: 嘌呤霉素(Puromycin)
克隆菌株: Stbl3
备注: pLVX-PAmCherry-C1载体表达的目的蛋白N端融PAmCherry荧光蛋白;
CMV启动子驱动目的蛋白的过表达;
可用流式细胞仪筛选。
产品目录号: 632587
稳定性: 稳表达
组成型: 组成型
病毒/非病毒: 慢病毒

载体质粒图谱和多克隆位点信息

pLVX-PAmCherry-C1载体图谱



pLVX-PAmCherry-C1多克隆位点

pLVX-PAmCherry-C1载体特征

载体简介

载体序列

LOCUS       pLVX-PAmCherry-C1  8770 bp    DNA   circular  SYN        8-AUG-2011
COMMENT     Created by Clontech Laboratories Inc.
COMMENT     Cat. No.632587
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     source          1..8770
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..635
                     /label=5'\LTR
                     /note="color: #000080"
     misc_feature    685..822
                     /label=Packaging\signal
                     /note="color: #66cc99"
     misc_binding    1303..1536
                     /label=RRE
                     /note="color: #009966"
     misc_feature    2028..2151
                     /label=CTS/cPPT\(DNA\flap)
                     /note="color: #66cc99"
     promoter        2185..2788
                     /label=CMV
                     /note="color: #66cc99"
     CDS             2806..3513
                     /label=PAmCherry
                     /note="color: #ff9966"
     misc_feature    3530..3594
                     /label=MCS
                     /note="color: #66cc99"
     promoter        3596..4104
                     /label=PGK\promoter
                     /note="color: #66cc99"
     CDS             4125..4724
                     /label=Puro(R)
                     /note="color: #66cc99"
     misc_feature    4738..5329
                     /label=WPRE
                     /note="color: #66cc99"
     LTR             5532..6168
                     /label=3'\LTR
                     /note="color: #000080"
     rep_origin      complement(6638..7311)
                     /label=pUC\ori
                     /note="color: #ff9966"
     CDS             complement(7456..8452)
                     /label=Amp(R)
                     /note="color: #ff9966"
BASE COUNT     2178 a      2265 c      2330 g      1997 t 
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 
     2761 tccatagaag acaccgactc tagctagagg atcgctagcg ccaccatggt gagcaagggc 
     2821 gaggaggata acatggccat cattaaggag ttcatgcgct tcaaggtgca catggagggg 
     2881 tccgtgaacg gccacgtgtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc 
     2941 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcac ctgggacatc 
     3001 ctgtcccctc aattcatgta cggctccaat gcctacgtga agcaccccgc cgacatcccc 
     3061 gactacttta agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaaattcgag 
     3121 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggtga gttcatctac 
     3181 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc 
     3241 atgggctggg aggccctctc cgagcggatg taccccgagg acggcgccct gaagggcgag 
     3301 gtcaagccga gagtgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc 
     3361 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaaccg caagttggac 
     3421 atcacctcac acaacgagga ctacaccatc gtggaacagt acgaacgtgc cgagggccgc 
     3481 cactccaccg gcggcatgga cgagctgtac aaggctaccg gactcagatc tcgagctcaa 
     3541 gcttcgaatt ctgcagtcga cggtaccgcg ggcccgggat cccgcgactc tagataattc 
     3601 taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg 
     3661 ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt 
     3721 aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta 
     3781 gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta 
     3841 gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc 
     3901 ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga 
     3961 aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg 
     4021 tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc 
     4081 tcttcctcat ctccgggcct ttcgacctgc agcccaagct taccatgacc gagtacaagc 
     4141 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg 
     4201 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg 
     4261 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg 
     4321 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg 
     4381 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc 
     4441 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg 
     4501 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc 
     4561 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc 
     4621 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg 
     4681 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaccgcgt ctggaacaat 
     4741 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct 
     4801 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg 
     4861 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg 
     4921 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt 
     4981 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt 
     5041 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg 
     5101 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc 
     5161 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat 
     5221 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc 
     5281 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattaattct 
     5341 gcagtcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg 
     5401 ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc 
     5461 aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag 
     5521 aaaagagggg actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt 
     5581 ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg 
     5641 tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg 
     5701 tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga 
     5761 tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc 
     5821 acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca 
     5881 agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc 
     5941 gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta 
     6001 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa 
     6061 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac 
     6121 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt 
     6181 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggcct 
     6241 tgacattgct agcgttttac cgtcgacctc tagctagagc ttggcgtaat catggtcata 
     6301 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag 
     6361 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg 
     6421 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca 
     6481 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc 
     6541 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 
     6601 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 
     6661 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 
     6721 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 
     6781 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 
     6841 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 
     6901 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 
     6961 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 
     7021 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 
     7081 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac 
     7141 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 
     7201 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 
     7261 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 
     7321 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 
     7381 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 
     7441 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 
     7501 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 
     7561 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 
     7621 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 
     7681 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 
     7741 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 
     7801 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 
     7861 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 
     7921 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 
     7981 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 
     8041 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 
     8101 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 
     8161 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 
     8221 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 
     8281 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 
     8341 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 
     8401 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc 
     8461 gggagatcaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa 
     8521 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca 
     8581 atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac 
     8641 ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct 
     8701 gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa 
     8761 acttagtagt 
//

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