| 质粒名称: | pTNS2 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 64968 |
| 存储实验室: | Herbert Schweizer |
|
载体骨架基本信息 |
|
| 载体名称: | pUC18R6KT |
| 载体出品公司: | Herbert Schweizer |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 2921 bp |
| 完整质粒大小: | 9619 bp |
| 载体修饰: | -- |
| 载体类型: | 细菌表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | tnsABCD |
| 物种来源: | -- |
| 插入大小: | 6698 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pTNS2 was a gift from Herbert Schweizer (Addgene plasmid # 64968 ; http://n2t.net/addgene:64968 ; RRID:Addgene_64968) |
| 参考文献部分: | A Tn7-based broad-range bacterial cloning and expression system. Choi KH, Gaynor JB, White KG, Lopez C, Bosio CM, Karkhoff-Schweizer RR, Schweizer HP. Nat Methods. 2005 Jun;2(6):443-8. 10.1038/nmeth765 PubMed 15908923 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pTNS2 9619 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Tn7 transposase expression.
ACCESSION .
VERSION .
KEYWORDS pTNS2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9619)
AUTHORS Choi KH, Gaynor JB, White KG, Lopez C, Bosio CM, Karkhoff-Schweizer
RR, Schweizer HP
TITLE A Tn7-based broad-range bacterial cloning and expression system.
JOURNAL Nat Methods. 2005 Jun;2(6):443-8.
PUBMED 15908923
REFERENCE 2 (bases 1 to 9619)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9619
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(29..51)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 151..170
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 364..386
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 378..395
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 379..395
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
mobile_element 6709..6933
/mobile_element_type="transposon:Tn7"
/note="mini-Tn7 element (right end of the Tn7 transposon)"
primer_bind complement(7145..7161)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7145..7161)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(7158..7180)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 7169..7185
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7193..7223)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7238..7259
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(7376..7393)
/label=L4440
/note="L4440 vector, forward primer"
oriT 7635..7743
/note="incP origin of transfer"
rep_origin 7794..8150
/label=R6K gamma ori
/note="gamma replication origin from E. coli plasmid R6K;
requires the R6K initiator protein pi for replication"
CDS complement(8559..9419)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9182..9201
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9420..9524)
/gene="bla"
/label=AmpR promoter
primer_bind 9592..9610
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcg
421 actctagagg atcccccgat tattttggct cgttgaagat ccgatgcttc aaactcaatc
481 acacgatccg ccttatttat ttgctttgtt gtactgttaa ccaccttagc tcgacttaat
541 aaaggcaaat gagtaaatcg agtccatttc cgttcttgca ttggattaaa ccaaacaaaa
601 atacgccact ccttatgacc cgagttacgg aataaatgac caatatgtac aacctgcaca
661 ttgtcattaa atgtagctag cctaaccact cactcttccc cataaaccat ttccaagaat
721 ctttgtgctt cctcagttat ccgctcttta gataacgttg cacttcttaa taatcgccag
781 cgcctaagct caacatcttc ctgtttaagc ttaataaaag cttggctaat ccgtctaatt
841 tgataatctt ccacactctc tgagtaacgc tttaagcaaa gcgctaccaa aggcagtttc
901 tgtagatttt ttgcaagaga ggttccgtta ggagtttgct ttaacagcca gctcgatgtc
961 gctcttgggt gatcaaggct actatctaga cgctttatga ttcttaatag ttgtcgtaca
1021 gcaattcgat ctctttggtt ccaatcaact ctaggggcgg gtgccagacg ctcttgttga
1081 tgctgttgat tccagtgaac tagccaatcc ctgtcatgtc ggtaaagcca agcgtatagc
1141 accccaccct ctaaagactg tcttgccgcc ttaattcctt ggtatttatg cactagttgc
1201 tgccagtctt tatgcttaac agataaatct tcagagttag gttgcacaga ctggctaaca
1261 ggtcttgtcg ttatagagtg ctcagtaaga gcacttgcct gctgtagcgc ttctataacc
1321 gttagttttg gcaataaggc ttgccacaca atactatgct gtaaataact aaaggctttt
1381 ctatgcttac ggaatatact tttcagccaa cacgtgtcct tgttctctgc caactttaaa
1441 tccagtttct ctagtgcctc atcactaaaa gtctgcctca ctctctccgc caccaagtca
1501 tgacgaatgt gcttgctttt ggttagccct agatcctgcg ctaagcgctg ataaaacagc
1561 gtccactgct caaggcttgg ggaaagctct tgcgctcgtg gagcatctaa cagaggggct
1621 atataagcag ctagtgctgt taattgagat agggagtctt tggggtagtc tgaaagcagc
1681 tcagtatgac ccaaagccca aaattgatgt cggtgatcat ctacagctct atcaaagaag
1741 actaaagcac cgtgttttgg acaatatggc aaagcgggca aataccaatc tcgttgccaa
1801 aaggcttccc catacctatt tagctgaaga gcaacgcaat cagggcagta tctaaagcgg
1861 ttatcgctct taactctaga agcagcgact cctagcatta aatgcaccgc accttgcgct
1921 tggtactcca ttaaccgaat agcttcgtct cggcgctcct tgcctacaaa cggagcatat
1981 aaagggaata aggtatgctc ataaataagc tgctgaacag cgtaacgtcc tgtttgatgt
2041 agatgtcttg ctatcacacc taaatgcgag ggcagaccta aggtagcgac caccttgcgg
2101 ttgccataca cctcatccaa cagctgctta ggactaacaa tcccttgata aacgcctgcc
2161 cgtgcaatag tgctataaat cagctcattc gagtacggaa caggaaagtt tctcatggct
2221 aacctgcttg tttaaataag ctcgccatat ccactattac ccctttccct tttaaccgtt
2281 catgcatggt tttttcaggt tggcgttgtg aatagatata acgtaaatcc gtatcaggca
2341 agctatccca gtcgctgggc ttgactacct ttatagccga aaccttttta ctcttggagg
2401 gcttttctag ttctgacact accgtttcgc cttccatcaa ccactgcaaa acaagaggca
2461 gtaacttttg tcttgtcatc gttggattct ggctaaacgc ttttttaata gtgggaatta
2521 acaggcttga atcgtaatcc tctttcagca tcagatataa atgacgctga tcttcggtat
2581 ctaactcttg aagggctttt tcttctggtg tttgttcttg tatcgctgcg atatctagct
2641 gaagttggat taaccgttta tcaatctcgg gaacgactag atcagaataa cgagcaatgc
2701 gttctgggat acccgagcgt aatgcctcta gcatggggtg cacaggcttt aactcatctt
2761 gatacacttg ccgcaataaa ccagcggtaa tacgctcatt gcctaaagct agcgcacgga
2821 gctgagcgag tacaaaaagt tttactacaa tgtccatcac tccttggctt agctcatacc
2881 acacatcacg gacctcatcc gataacagcg catctttgcg ttgtaaaagc tgtaattgcc
2941 aaagattatc cgtaaaagcg atccactctt gattgggctt tccacgttgc gtttgttgta
3001 taggatccca gaatatagct ccaaaccctg cccctctacg tgcagaccgc aaatcagcct
3061 caaaaatctc tcgtgcttta ggggtaccaa tcaacatcac tggtacgcca ataatattca
3121 ccatcgtcac aaaaaagttc agcatctctt gagatccacc cgaacgagag cggcttaaat
3181 gctgaatttc atcaataacc aacaacccta aagcatgtgc attggctatt tgcgacatca
3241 aagccaacat ggtttctata ccatgacgtt ttaagccata acgacgctca tagttcgagc
3301 ccaaggctcg atccaacgct ctgaaaaaat tcaagcagat ttcttttagc gaaccattat
3361 gcgagcagtc tattttcaaa tacaccacct gctctacatt gagttcacga tggtaaatca
3421 cctgaggata cgtggctaga atacgatgaa gagaggtcgt cttcccacta ccagaacaac
3481 caattaacaa taagctttgt gccgtagatc gtgcctcctc aaagcgaaat gtctccaact
3541 ctcccgtttg aacacgctca taaccatttt gtaaatgctt ttgtaaatct cctgttttag
3601 gatttctgcc tacgtagcca cctcgaatca tgaccgaaat acgctcactt agtagcaaat
3661 gcgtacctaa tggctgaaaa tagtcatctg gaatacgaca aatggtatga gctctgataa
3721 cacgggactt ttgcaagtca gaggaagtaa gctgtaaaga ggatttcagt gatgcagcac
3781 tattcactga ctcttgtaat ggtggtaagg cctcgataaa aggattatca cgataagcct
3841 ctacccccgt atcacgataa actgcttgaa tccgggtagc actcatgact catccttttc
3901 tggtggatcc tgaaataatt caggcacgta tgtgggtagg ctgtaatctt cttgatcatc
3961 cgcttccact gcgttgaaag gaataacttt agcctcatca cctgaagagc ttggcttcaa
4021 atgctccgca cgttttttac gctccgaggt cacggcttct tttttattag ttttaatctg
4081 cttaatgcgt gttgatttgg gctcagtagt actgggcgtt aacttattcg ctttctgaat
4141 ggtttgctga ataaacgcct caagctccct gcgtttagtt aactcatcct gcttcgcatt
4201 ggctttattg tgtttttctt gtgcttgtat atcccaaacc tcccaaaatg agagaccttt
4261 aaactgccga ctacgttccg tcagattaca gcgccaaaat acacggctgc caacttgcgg
4321 aaacaaataa atcgtatcaa ccagcactgg gtcataagcc gcttctaaat gttgaggtct
4381 agctatatca gtgctccgct gcaaccaacc ctcacgcaga atctctgacc ccgagtaata
4441 caaaccccac aaattaacgc caaatgaaga aatagagacc tttcggcgag gcagtaacgc
4501 tactcgcaac tgctcttgct ccacagccct taaactacct gtacgatgct gcatacccca
4561 ttgccatagc tggacaggaa tagacggtaa atctgtagga aaatcagcat ctcgatcgta
4621 tttatccatc accagatggt tatttctgaa taagatcgta cgcaaaataa tttgtgtgaa
4681 ctcaaatacc gacagagatg catctaacct atagtctgtt tcaccatggc ttttgatccg
4741 actgccctct acaatgccag gtgcaaagga cttaaactcg gcttgtagtg ttctaaaagt
4801 gctttccact atgcctttag catcgccacg tctaggtgga gcactttcca ctcgcacatt
4861 aaaactagaa actaaggctt cgacctgatg actcattaat tcgccacggt ccgctagcaa
4921 cacatctggc aaacctacac acggccagtc tgagctacta atctcaatat catgctgggc
4981 acaaatggcc gttttgtcag agcaagcatt tacaaaagcc tgcatcgcca ccacataaga
5041 cggattttca aagccgatat aaaagcccgt gatcatccga ctaaacacat caatcacaat
5101 gtaaagcgtt ggtcttccta tgattttttg gcgatcatga tgatccacta aataaatatc
5161 agcaatcgtg gcatcaatct cataacgact cccagggcct aacgcctgag aagtggctgt
5221 actacttaag ggtcgtacgt cttttttata tacccctgct ttaactctag actttaagcg
5281 ctgagcttta gggtattctc gatcataaaa ataacgaaac tgacgtagtg ttgggtaatc
5341 ctcttgggga atgcgaggaa aatactgagc aaacaagtcc acaaatcgtc tataggcaac
5401 ggtggtcttt gtaccttttt gatttaacag gtgcttttct atggtcaacc taaaaaggcg
5461 ttcaatctcg ggcgttacct tggttccttc acccttacca tattctctgg ctcggccaat
5521 ctttgctgtt cctgtcgctg aacgtctttc ccctggtgca ccgctgtttt tgtagtcagg
5581 aattaatgca ttaggcgttt gaccacgctg ccagtaacgg cgtaacaact tataaactgt
5641 agccttagta accttatgtt cttggaccac atgctcaacg agttcgcttc tgactttagg
5701 gtcgaaacga tccttactat taataatagg aagaattttt cgataatcct cctcgcgctt
5761 ctggaagctg acagaatcta cagacggctc ttctaaatca agatgtacat aagggtcatc
5821 agttctaact aagcggcctt catctaaata ctgcattagc aacaactcag ccctagcttg
5881 tggaactcct ttatcagcgc ttatttgcat ccagacaact tggccatcct ctatagccaa
5941 aatgcgatac ggatcattat caaatagcac aacctcatta atttgccaca tagcgcaact
6001 cctccatatt cactacttgg ctaatacaga gatctgcaca cttatttgcc ctgaaagact
6061 tataaatatt gaacttaata aaaccatttg cggttaaggc tcgtatctca ctcaatgttt
6121 tgcctaactc caaatcataa gcaatatcaa cctgcttaca gacattgata atgttttcat
6181 ctcctttttc ttgcaggata tgggccaatg gggatagttg tgctaaaagc tccgcagaaa
6241 cttcttctgt tttcactgaa taaagccatt caatattttc ttttactacg ggatttattt
6301 ctttatcagt aaaaatgaac caaggaattt gcttttgctg ccaatagcga cgctctagtt
6361 ctagtttttc taaggtacgc tcgtcttgta aggctgctgc aggtttgact tgaatagcaa
6421 actgctcaaa aggaccatct ttgcagtcca ctaaaaaatc agtagacata acctgatcta
6481 caccacgaat aacaggatgc ttaataccac tatctattgc aatctgcctg gtatcactag
6541 gtaataaggg gaactgctcg cgtatatcta gcacgctgct ctcccactca agactgagaa
6601 aaacagcaag ctctaagtca gatagcaaat gatggactcg tcccgtctta tgagaataaa
6661 tacggtggga acgacctgaa gaaggaactt cttgtactgt tagccatgga atatagtctt
6721 taccatgccc ttggccacgc ccctctttaa tacgacgggc aatttgcact tcagaaaatg
6781 aagagtttgc tttagccata acaaaagtcc agtatgcttt ttcacagcat aactggactg
6841 atttcagttt acaactattc tgtctagttt aagactttat tgtcatagtt tagatctatt
6901 ttgttcagtt taagacttta ttgtccgccc acacccgctt acgcagggca tccatttatt
6961 actcaaccgt aaccgatttt gccaggttac gcggctggtc aacgtcggtg cctttgatca
7021 gcgcgacatg gtaagccagc agctgcagcg gaacggtgta gaagatcggt gcaatcacct
7081 cttccacatg cggcatctcg actctagagg atccccgggt accgagctcg aattcgtaat
7141 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
7201 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
7261 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
7321 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
7381 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
7441 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg ttaaggtata
7501 ctttccgctg cataaccctg cttcggggtc attatagcga ttttttcggt atatccatcc
7561 tttttcgcac gatatacagg attttgccaa agggttcgtg tagactttcc ttggtgtatc
7621 caacggcgtc agccgggcag gataggtgaa gtaggcccac ccgcgagcgg gtgttccttc
7681 ttcactgtcc cttattcgca cctggcggtg ctcaacggga atcctgctct gcgaggctgg
7741 ccgataagct ctgataacgc aggaaagaac atgggcagtt caacctgttg atagtacgta
7801 ctaagctctc atgtttcacg tactaagctc tcaagtttaa cgtactaagc tctcatgttt
7861 aacgaactaa accctcatgg ctaacgtact aagctctcat ggctaacgta ctaagctctc
7921 atgtttcacg tactaagctc tcatgtttga acaataaaat taatataaat cagcaactta
7981 aatagcctct aaggttttaa gttttataag aaaaaaaaga atatataagg cttttaaagc
8041 ttttaaggtt taacggttgt ggacaacaag ccagggatgt aacgcactga gaagccctta
8101 gagcctctca aagcaatttt gagtgacaca ggaacactta acggctgaca accggctggt
8161 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
8221 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
8281 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
8341 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
8401 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
8461 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
8521 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
8581 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
8641 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
8701 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
8761 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
8821 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
8881 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
8941 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
9001 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
9061 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
9121 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
9181 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
9241 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
9301 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
9361 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
9421 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
9481 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
9541 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
9601 atcacgaggc cctttcgtc
//
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