pLentG-KOSM

价格:1250元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: 爱迪基因
载体名称: pLentG-KOSM
质粒类型: 慢病毒系统载体
高拷贝/低拷贝: --
启动子: CMV
克隆方法: 多克隆位点,限制性内切酶
载体大小: 13kb
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: GFP
载体抗性: 氨苄青霉素(Ampicillian)
筛选标记: --
备注: --
产品目录号: --
稳定性: --
组成型: --
病毒/非病毒: 慢病毒

载体质粒图谱和多克隆位点信息

pLentG-KOSM载体图谱



载体简介

pLentG-KOSM is a simple lentiviral vector in which defined factors are in-frame fused into a single open
reading frame (ORF) via self-cleaving 2A peptides and are controlled by a CMV promoter. The mouse
transcription factor ORF is followed by IRES-GFP as a reporter for viral transduction. This polycistronic
expression system can efficiently reprogram mouse or human somatic cells into iPS cells; for example, in
infected MEF (GFP+), the reprogramming efficiency is around 1%, which is at least 10-fold higher than
the reprogramming method using a pool of viruses in which each virus expresses a different stem cell
factor gene. Notably, most of the iPS cells generated using the pLentG-KOSM system contain only a
single copy of the viral vector (Ref. 1).

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