Lenti-Hb9-RFP

LOCUS       Lenti-Hb9-RFP      12351 bp DNA     circular SYN 25-AUG-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12351)
  AUTHORS   Marchetto MC, Muotri AR, Mu Y, Smith AM, Cezar GG, Gage FH
  TITLE     Non-cell-autonomous effect of human SOD1 G37R astrocytes on motor 
            neurons derived from human embryonic stem cells.
  JOURNAL   Cell Stem Cell. 2008 Dec 4;3(6):649-57.
  PUBMED    19041781
REFERENCE   2  (bases 1 to 12351)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 25, 2024 from SnapGene Server 7.0.3
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell."; date: "2008-12-4"; volume: "3(6)"; pages: "649-57"
FEATURES             Location/Qualifiers
     source          1..12351
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1677..1910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2095..2139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2391..2507
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6138..6176
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             6251..6931
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MVASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGTLIYKVKFRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFL"
     primer_bind     complement(6384..6402)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(6434..6454)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     6652..6671
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     6846..6869
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    7158..7746
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7211..7231)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7629..7640)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8153..8333
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8359..8376)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8365..8589
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8635..9063
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8722..8741)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8932..8953
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9072..9092)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9077..9406
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9257..9392
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9319..9338
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9454..9501
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9520..9894
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10024..10145
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10061..10080)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10115..10134
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10194..10210)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10194..10210)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10207..10229)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10218..10234
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(10242..10272)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10287..10308
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10425..10442)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10596..11184)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10676..10695)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11355..12215)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11978..11997
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12216..12320)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2281 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2341 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcaac ttttaaaaga
     2401 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     2461 atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc gatgcggccg
     2521 ctcgactcga tcctgtctcc ctggccagga gtctggggtc cgcagatata ttacaacaaa
     2581 attactaatt ggaaaatgaa attgtcaata aagcacaccc cacgcgtgta ctatatcttt
     2641 aaatattatg atgtcttatt ggtcccattt ccttacaata atttattgca ataaaacaga
     2701 cactgcaggt ccccttaaat tgtcttttca taatgaataa atttaagcag gctaattaat
     2761 atataaacta gctcaatttg tcaagttgat ttgtatttta gttaattgtg aaagtaatta
     2821 ccacatggtc aaattaacag ctttctggaa atgaccaagc ctgaggtttt atttccttcc
     2881 tgggtgaaga aaattcattt ttccaagctc ttgatgtgat gaataaaagt cataaatctg
     2941 ggtgattggt gcaggcagag tctaaatggc ttcatatttc attttaggtt taatagaaat
     3001 attcatgctc tgttttaatg aaattaaatt gaagggggat ggggctctgg aggcggccct
     3061 ggctgggggc ttaaagggac agtgcacttt tcctttcttc atgcaagggc cagggctaga
     3121 ggtccctgga ctcagtcaag aagtggagag agctgaggga ggcctgggag gcccttattc
     3181 tgtacccagt gtagcacacc tgcagccctg attggggggg ggggcggggg caattcacac
     3241 cctgtattct caccaggcta aagtttcttc tatctggttc cttctctcca aaaaatcagt
     3301 cagaagggac ctcccttggc tgagagtaga agtgaggtgg tcagcgtgct gcagacaagc
     3361 ctgtcctcca tctgcctaag gggtctgata tcctacacca agccccccct accccttctg
     3421 gagacacata ggagataagc attttcaaaa ccaccatggc actctcctgt tctcccctgc
     3481 accccttcct cccagactcc atcctgcctg aggatgattt ggtccaactt gaataataga
     3541 gtactctctt tccacatctt tccacatcgg aaagagtagt ccgggatcca gtccacctgc
     3601 agccctgatt aaaggaagga aggaagacta gaggcgcagt tctacctatt atacttattt
     3661 taaggttccc aggtcagaga tgcctcatgg acatttctcc ctaaggcagg tcctgaccag
     3721 ccctccccct gaatggtgct gatctgtgac ttgttgcttc tattgaacaa atacagcttt
     3781 gatttgtttc cagccagagt ggttagctga tgaattgaca aaaactaatc agctttattg
     3841 ggaaacaggt ttaagggcac ggacgtgtca ataacgctca gcctgacccc ctcttccatt
     3901 agctaggcag gctgattaga gtaagaagac cctctcgccg attgcaccga aattgctttc
     3961 ataaactcac aatattagca gtcgatgtga gcagctaatt tagagaagac atccaggccc
     4021 gtcacatcga tcatcgggtt tatgtatcat tatctcattt acagaatgtc gcagtgtaat
     4081 acatgttttt ggcaataggc ttggccgctc gccggagccg cgcgctgttc ctttcggcca
     4141 cactgcaggg gacttcctca acaatctacg atgtctcttc gcgtgaacaa ggaggcactt
     4201 tgcacacata tgcacacttg cgcccaggct ctcagtgcgc gctcagtgca cacatgcacg
     4261 tggacacatg ctccgacata ctacctgagc tgcctttcct tctcatgcgg ggatgctggc
     4321 attgctctct cctgcgagcc cctggtctga agcccaatgc cggacgctca ccaaccttgg
     4381 agtcaacctc ggagagctca aggttcggtt ctgtcaccag catacagaga tggtgggtgg
     4441 cggcgcaatc agccaccttc tctcaaggct ttggtcgccc agttggcggg tgtccagaaa
     4501 tccacaggcc tgcgaaggtc cttctccgcc ctcccaggct ccagcttgac ctgccctgcc
     4561 ctgggcgggc ctttctcgca acacttccag gctcagccag atgcgcctgg tcttagggag
     4621 tgggccacct gttcctttgg gtaattattt taatgtaagg ggtctacttc aggcctcctg
     4681 cagtgacaga cggcagcgga gctgcaaagc gctctcagcc tttcagtgga atttcccaga
     4741 tgggccaagg ggaggccagg gcgggtcacc aggggccctt taacgagttc attagccaaa
     4801 ttatcttgcc tgcagcaagg accccagaga ctgtagggag agtaggggag aaggtgggtc
     4861 tttctccctc cctgctagtc tgcaagaaca gttgtgggaa ggaaagagaa aggacatcct
     4921 ccccaaaggg gcctaggaac actaaaggca aagatggcat ctctgatgtg acttatgcgc
     4981 cagaccagcg aggtggtgga gtggtgggaa ggaatcataa tgattaggaa acacggagat
     5041 tctaacaacc agggcactga cccatattgt ggaacaaacc tagtgcagaa atcaaagtca
     5101 gagcccctgg aactttattc tgtgcttctt aagcaaagaa cctggacatc ttggagcctt
     5161 ggtctgtccg tccatctgta gagtagaagg taggtgtgtt tccttccaca atttgtgaga
     5221 tcactgccct aagaaaagca actctgggaa gagcttctgg catgaccctg ttgggtcctt
     5281 cgggccccct tactcctgtg gtaggaggct ttggcaggtt gaggtgagcc tgatcctaga
     5341 tgcctgtgct ggaaaatgaa cccaactctc acctgtcgtc gcgcccccca cccccacccc
     5401 acagctgtga ggcgctgctc tggaggtagc tcagcatccc cgcccctcct ccttgtcctc
     5461 actctgcctc ctgtagtgtt agcttctgct acctcagcaa gtagtgagtg aatagaccaa
     5521 ttagccagga cttcctgcca ttatctgctg tagacttggg cattagtacg ccctgattca
     5581 ccataatttc aagtcagaac acactaaaaa attcactcac cgaggcatta aaatgttccc
     5641 agacaacgtc tgtgtttgtg taactgggtt tcttcccttc gtgtttgatg cacaccagca
     5701 tcatataggt cagtggtgga gtccaggcta ggcagacaat ttttgtcgct ttttgtttgt
     5761 ttttatattt ttaaaacatg aattgcattg tttcccggtg gttgtaaact ttgtttctgg
     5821 ggcttcagta ttgccagtgt aatcttcttc tgcttgatca ggggcaaaca agtgacttag
     5881 aaagttgtgg gagtgcccta agaacagtag ccagagcctt tacagagcca ggctttgtgt
     5941 cctaaacgta catcgcccca gctatgggaa gctgggttat caccttcaaa gcagaccctt
     6001 tctgtggatg acttaccaag ccctgcaggt gggtagtgga ggtggggaga gggctgcaca
     6061 gcctgcttgg agcttgcttc tgttctttgt cctgcccatg atatgaagct cctatacaat
     6121 gaatttgcag taagatcggt aggcgtgtac ggtgggaggt ctatataagc agagctggtt
     6181 tagtgaaccg cagatccggc gcgccaagct tcgaattctg cagtcgacgg taccgcgggc
     6241 ccgggatccg atggtggcct cctccgagga cgtcatcaaa gagttcatgc gcttcaaggt
     6301 gcgcatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
     6361 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggcggcc ccctgccctt
     6421 cgcctgggac atcctgtccc cccagttcca gtacggctcc aaggcgtacg tgaagcaccc
     6481 cgccgacatc cccgactaca agaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     6541 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg
     6601 cacgctgatc tacaaggtga agttccgcgg caccaacttc ccccccgacg gccccgtaat
     6661 gcagaagaag accatgggct gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt
     6721 gctgaagggc gagatccacc aggccctgaa gctgaaggac ggcggccact acctggtgga
     6781 gttcaagacc atctacatgg ccaagaagcc cgtgcagctg cccggctact actacgtgga
     6841 caccaagctg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgagcg
     6901 ctccgagggc cgccaccacc tgttcctgta ggaattctgc agatatccat cacactggcg
     6961 gccgcgactc tagatcataa tcagtttaaa ctctagagga tccaccggtg gccgcctggg
     7021 cccgttaacg tctcgagttt aaacctggaa aaacatggag caatcacaag tagcaataca
     7081 gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt
     7141 ccagtcacac ctcagacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     7201 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     7261 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     7321 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     7381 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     7441 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     7501 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
     7561 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     7621 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     7681 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     7741 ccccgcctgg aattcgagct cggtaccttt aagaccaatg acttacaagg cagctgtaga
     7801 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     7861 acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg attggcagaa
     7921 ctacacacca gggccaggga tcagatatcc actgaccttt ggatggtgct acaagctagt
     7981 accagttgag caagagaagg tagaagaagc caatgaagga gagaacaccc gcttgttaca
     8041 ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtattagagt ggaggtttga
     8101 cagccgccta gcatttcatc acatggcccg agagctgcat ccggactgta ctgggtctct
     8161 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     8221 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     8281 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagggccgc
     8341 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     8401 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     8461 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     8521 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     8581 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc
     8641 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     8701 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     8761 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     8821 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     8881 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     8941 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     9001 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     9061 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     9121 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     9181 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     9241 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     9301 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
     9361 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc
     9421 ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc ggcatagtat
     9481 atcggcatag tataatacga caaggtgagg aactaaacca tggccaagtt gaccagtgcc
     9541 gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac cgaccggctc
     9601 gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga cgacgtgacc
     9661 ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg
     9721 gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac gaacttccgg
     9781 gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc
     9841 ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg
     9901 ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     9961 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
    10021 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
    10081 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
    10141 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
    10201 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
    10261 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
    10321 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
    10381 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
    10441 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
    10501 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
    10561 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
    10621 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
    10681 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
    10741 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
    10801 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
    10861 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
    10921 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
    10981 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
    11041 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
    11101 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
    11161 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
    11221 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
    11281 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
    11341 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
    11401 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
    11461 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
    11521 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
    11581 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
    11641 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
    11701 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
    11761 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
    11821 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
    11881 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
    11941 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
    12001 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
    12061 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
    12121 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
    12181 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
    12241 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
    12301 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt c
//