pDC411

价格:1500元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Microbix Biosystems
载体名称: pDC411
质粒类型: 腺病毒表达载体
高拷贝/低拷贝: --
启动子: --
克隆方法: --
载体大小: 5574 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄青霉素(Ampicillin)
筛选标记: --
备注: 克隆宿主菌:DB3.1
产品目录号: --
稳定性: --
组成型: 组成型
病毒/非病毒: 腺病毒

载体质粒图谱和多克隆位点信息

pDC411载体图谱



载体简介

载体序列

LOCUS       pDC411       5574 bp dsDNA    Circular
DEFINITION  Puc based shuttle plasmid  for Ad vector rescue via homologous recomb. Ng et al. Hum. Gene Ther. 11: xxx, 2000.
COMMENT     This file is best viewed with a free Vector NTI Viewer:
COMMENT     http://www.informaxinc.com/view/viewer.html
COMMENT     VNTREPLTYPE|Plasmid|
COMMENT     VNTEXTCHREPL|Bacteria|
FEATURES               Location/Qualifiers
     misc_feature    515..2785
                     /note="Main body of the Ad5 sequences present in this vector system with E1 deleted but with a normal packaging signal."
                     /label=Ad\5\E1\deleted
     CDS             600..1019
                     /note="Protein IX open reading frame only, does NOT include promoter/ terminator etc."
                     /label=pIX
     rep_origin      3264..3564
                     /label=ori
     misc_feature    5115..453
                     /label=Ad
     CDS             complement(4014..4830)
                     /label=amp
     CDS             5484..1
                     /label=ITR
     CDS             complement(2..102)
                     /label=ITR
ORIGIN
        1 ttcatcaata atatacctta ttttggattg aagccaatat gataatgagg gggtggagtt 
       61 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg 
      121 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg 
      181 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt 
      241 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa 
      301 gtgaaatctg aataattttg tgttactcat agcgcgtaat atttgtctag ggccgcgggg 
      361 actttgaccg tttacgtgga gactcgccca ggtgtttttc tcaggtgttt tccgcgttcc 
      421 gggtcaaagt tggcgtttta ttattatagt cagntctaga gaattcaggc ctgctagcag 
      481 atctaagctt gagctcgtcg actcgaagat cgatctgggc gtggttaagg gtgggaaaga 
      541 atatataagg tgggggtctt atgtagtttt gtatctgttt tgcagcagcc gccgccgcca 
      601 tgagcaccaa ctcgtttgat ggaagcattg tgagctcata tttgacaacg cgcatgcccc 
      661 catgggccgg ggtgcgtcag aatgtgatgg gctccagcat tgatggtcgc cccgtcctgc 
      721 ccgcaaactc tactaccttg acctacgaga ccgtgtctgg aacgccgttg gagactgcag 
      781 cctccgccgc cgcttcagcc gctgcagcca ccgcccgcgg gattgtgact gactttgctt 
      841 tcctgagccc gcttgcaagc agtgcagctt cccgttcatc cgcccgcgat gacaagttga 
      901 cggctctttt ggcacaattg gattctttga cccgggaact taatgtcgtt tctcagcagc 
      961 tgttggatct gcgccagcag gtttctgccc tgaaggcttc ctcccctccc aatgcggttt 
     1021 aaaacataaa taaaaaacca gactctgttt ggatttggat caagcaagtg tcttgctgtc 
     1081 tttatttagg ggttttgcgc gcgcggtagg cccgggacca gcggtctcgg tcgttgaggg 
     1141 tcctgtgtat tttttccagg acgtggtaaa ggtgactctg gatgttcaga tacatgggca 
     1201 taagcccgtc tctggggtgg aggtagcacc actgcagagc ttcatgctgc ggggtggtgt 
     1261 tgtagatgat ccagtcgtag caggagcgct gggcgtggtg cctaaaaatg tctttcagta 
     1321 gcaagctgat tgccaggggc aggcccttgg tgtaagtgtt tacaaagcgg ttaagctggg 
     1381 atgggtgcat acgtggggat atgagatgca tcttggactg tatttttagg ttggctatgt 
     1441 tcccagccat atccctccgg ggattcatgt tgtgcagaac caccagcaca gtgtatccgg 
     1501 tgcacttggg aaatttgtca tgtagcttag aaggaaatgc gtggaagaac ttggagacgc 
     1561 ccttgtgacc tccaagattt tccatgcatt cgtccataat gatggcaatg ggcccacggg 
     1621 cggcggcctg ggcgaagata tttctgggat cactaacgtc atagttgtgt tccaggatga 
     1681 gatcgtcata ggccattttt acaaagcgcg ggcggagggt gccagactgc ggtataatgg 
     1741 ttccatccgg cccaggggcg tagttaccct cacagatttg catttcccac gctttgagtt 
     1801 cagatggggg gatcatgtct acctgcgggg cgatgaagaa aacggtttcc ggggtagggg 
     1861 agatcagctg ggaagaaagc aggttcctga gcagctgcga cttaccgcag ccggtgggcc 
     1921 cgtaaatcac acctattacc gggtgcaact ggtagttaag agagctgcag ctgccgtcat 
     1981 ccctgagcag gggggccact tcgttaagca tgtccctgac tcgcatgttt tccctgacca 
     2041 aatccgccag aaggcgctcg ccgcccagcg atagcagttc ttgcaaggaa gcaaagtttt 
     2101 tcaacggttt gagaccgtcc gccgtaggca tgcttttgag cgtttgacca agcagttcca 
     2161 ggcggtccca cagctcggtc acctgctcta cggcatctcg atccagcata tctcctcgtt 
     2221 tcgcgggttg gggcggcttt cgctgtacgg cagtagtcgg tgctcgtcca gacgggccag 
     2281 ggtcatgtct ttccacgggc gcagggtcct cgtcagcgta gtctgggtca cggtgaaggg 
     2341 gtgcgctccg ggctgcgcgc tggccagggt gcgcttgagg ctggtcctgc tggtgctgaa 
     2401 gcgctgccgg tcttcgccct gcgcgtcggc caggtagcat ttgaccatgg tgtcatagtc 
     2461 cagcccctcc gcggcgtggc ccttggcgcg cagcttgccc ttggaggagg cgccgcacga 
     2521 ggggcagtgc agacttttga gggcgtagag cttgggcgcg agaaataccg attccgggga 
     2581 gtaggcatcc gcgccgcagg ccccgcagac ggtctcgcat tccacgagcc aggtgagctc 
     2641 tggccgttcg gggtcaaaaa ccaggtttcc cccatgcttt ttgatgcgtt tcttacctct 
     2701 ggtttccatg agccggtgtc cacgctcggt gacgaaaagg ctgtccgtgt ccccgtatac 
     2761 agacttgaga ggcctgtcct cgaccgatgc ccttgagagc cttcaaccca gtcagctcct 
     2821 tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc 
     2881 aactcgtagg acaggtgccg gcagcgctct gggtcatttt cggcgaggac cgctttcgct 
     2941 ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc 
     3001 aagccttcgt cactggtccc gccaccaaac gtttcggcga gaagcaggcc attatcgccg 
     3061 gcatggcggc cctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg 
     3121 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag 
     3181 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag 
     3241 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc 
     3301 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc 
     3361 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 
     3421 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt 
     3481 cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg 
     3541 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat 
     3601 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag 
     3661 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 
     3721 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc 
     3781 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta 
     3841 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag 
     3901 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga 
     3961 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa 
     4021 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa 
     4081 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc 
     4141 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga 
     4201 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa 
     4261 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt 
     4321 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg 
     4381 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc 
     4441 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg 
     4501 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag 
     4561 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt 
     4621 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt 
     4681 caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac 
     4741 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac 
     4801 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag 
     4861 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa 
     4921 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga 
     4981 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc 
     5041 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa 
     5101 ataggcgtat cactctaggc aaaatagcac cctcccgctc cagaacaaca tacagcgctt 
     5161 cacagcggca gcctaacagt cagccttacc agtaaaaaag aaaacctatt aaaaaaacac 
     5221 cactcgacac ggcaccagct caatcagtca cagtgtaaaa aagggccaag tgcagagcga 
     5281 gtatatatag gactaaaaaa tgacgtaacg gttaaagtcc acaaaaaaca cccagaaaac 
     5341 cgcacgcgaa cctacgccca gaaacgaaag ccaaaaaacc cacaacttcc tcaaatcgtc 
     5401 acttccgttt tcccacgtta cgtaacttcc cattttaaga aaactacaat tcccaacaca 
     5461 tacaagttac tccgccctaa aacctacgtc acccgccccg ttcccacgcc ccgcgccacg 
     5521 tcacaaactc caccccctca ttatcatatt ggcttcaatc caaaataagg tata 
//

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