| 质粒名称: | pAAVS1-P-CAG-DEST |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 80490 |
| 存储实验室: | Knut Woltjen |
|
载体骨架基本信息 |
|
| 载体名称: | pBluescript |
| 载体出品公司: | -- |
| 空载体大小: | 3000 bp |
| 完整质粒大小: | -- |
| 载体修饰: | AAVS1的同源臂；无启动子SA-T2A-puro选择盒；CAG启动子；网关目的磁带 |
| 载体类型: | 哺乳动物表达；供体载体（人类） |
| 筛选标记: | 嘌呤霉素 |
| 载体抗性: | 氯霉素和氨苄青霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | ccdB Survival |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | Gateway克隆 |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | -- |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pAAVS1-P-CAG-DEST was a gift from Knut Woltjen (Addgene plasmid # 80490 ; http://n2t.net/addgene:80490 ; RRID:Addgene_80490) |
| 参考文献部分: | Engineering the AAVS1 locus for consistent and scalable transgene expression in human iPSCs and their differentiated derivatives. Oceguera-Yanez F, Kim SI, Matsumoto T, Tan GW, Xiang L, Hatani T, Kondo T, Ikeya M, Yoshida Y, Inoue H, Woltjen K. Methods. 2015 Dec 18. pii: S1046-2023(15)30181-X. doi: 10.1016/j.ymeth.2015.12.012. 10.1016/j.ymeth.2015.12.012 PubMed 26707206 |
买家导航
pAAVS1-P-CAG-DEST载体质粒基本信息
pAAVS1-P-CAG-DEST质粒图谱载体图谱和pAAVS1-P-CAG-DEST载体序列质粒序列多克隆位点信息
pAAVS1-P-CAG-DEST质粒载体简介
pAAVS1-P-CAG-DEST质粒序列载体序列
LOCUS pAAVS1-P-CAG-DEST 9502 bp ds-DNA circular SYN 09-SEP-2021
DEFINITION Gateway Destination donor vector for AAVS1 targeting (puromycin
selection).
ACCESSION .
VERSION .
KEYWORDS pAAVS1-P-CAG-DEST
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9502)
AUTHORS Oceguera-Yanez F, Kim SI, Matsumoto T, Tan GW, Xiang L, Hatani T,
Kondo T, Ikeya M, Yoshida Y, Inoue H, Woltjen K
TITLE Engineering the AAVS1 locus for consistent and scalable transgene
expression in human iPSCs and their differentiated derivatives.
JOURNAL Methods. 2015 Dec 18. pii: S1046-2023(15)30181-X. doi:
10.1016/j.ymeth.2015.12.012.
PUBMED 26707206
REFERENCE 2 (bases 1 to 9502)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 9, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9502
/organism="synthetic DNA construct"
/mol_type="other DNA"
intron 105..1121
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
primer_bind 1045..1067
/label=pCAGGS-5
/note="Chimeric intron in CAG promoter, forward primer"
primer_bind 1129..1148
/label=pCAG-F
/note="Rabbit beta-globin intron, for pCAG plasmids,
forward primer"
protein_bind 1215..1339
/gene="mutant version of attR"
/label=attR1
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
promoter 1364..1394
/label=lac UV5 promoter
/note="E. coli lac promoter with an ""up"" mutation"
CDS 1448..2107
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
primer_bind complement(1514..1533)
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
CDS 2449..2754
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/label=ccdB
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
primer_bind 2633..2652
/label=ccdB-fwd
/note="ccdB gene, forward primer"
protein_bind complement(2795..2919)
/gene="mutant version of attR"
/label=attR2
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
primer_bind complement(3015..3034)
/label=Bglob-pA-R
/note="Rabbit beta-globin polyA region, reverse primer"
polyA_signal 3080..3135
/label=-beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
primer_bind complement(3134..3153)
/label=rbglobpA-R
/note="Rabbit beta-globin polyA, reverse primer. Also
called rb-glob-pA-term-R"
misc_feature 3691..4220
/label=HA-R
/note="right homology arm from the adeno-associated virus
integration site (AAVS1) within intron 1 of the human
PPP1R12C gene"
primer_bind complement(4366..4385)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(4367..4385)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(4392..4409)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(4392..4408)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4401..4423)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 4550..5005
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4637..4656)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4847..4868
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 5031..5135
/gene="bla"
/label=AmpR promoter
CDS 5136..5996
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(5354..5373)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 6167..6755
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 6656..6675
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 6909..6926
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 7043..7064
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 7079..7109
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7117..7133
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 7122..7144
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 7141..7157
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 7141..7157
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
promoter 7178..7196
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
misc_feature 7209..8012
/label=HA-L
/note="left homology arm from the adeno-associated virus
integration site (AAVS1) within intron 1 of the human
PPP1R12C gene"
misc_feature 8019..8044
/label=SA
/note="splice acceptor site"
CDS 8068..8121
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
CDS 8131..8730
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(8131..8150)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 8627..8647
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
primer_bind complement(8762..8779)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 8768..8992
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
enhancer 9011..9390
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
ORIGIN
1 gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc
61 ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgcgct
121 gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg ctctgactga
181 ccgcgttact cccacaggtg agcgggcggg acggcccttc tcctccgggc tgtaattagc
241 gcttggttta atgacggctt gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc
301 gggagggccc tttgtgcggg gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg
361 ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga gcgctgcggg cgcggcgcgg
421 ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt
481 gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag
541 cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct gcacccccct ccccgagttg
601 ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg gcgtggcgcg gggctcgccg
661 tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg ggcggggccg cctcgggccg
721 gggagggctc gggggagggg cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg
781 cgagccgcag ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt
841 cccaaatctg tgcggagccg aaatctggga ggcgccgccg caccccctct agcgggcgcg
901 gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc
961 cgcgccgccg tccccttctc cctctccagc ctcggggctg tccgcggggg gacggctgcc
1021 ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag
1081 cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg
1141 ttattgtgct gtctcatcat tttggcaaag aattcgcggc cgcggtaccg gcgcgccgga
1201 tcctgcaggt tatcacaagt ttgtacaaaa aagctgaacg agaaacgtaa aatgatataa
1261 atatcaatat attaaattag attttgcata aaaaacagac tacataatac tgtaaaacac
1321 aacatatcca gtcactatgg cggccgcatt aggcacccca ggctttacac tttatgcttc
1381 cggctcgtat aatgtgtgga ttttgagtta ggatccgtcg agattttcag gagctaagga
1441 agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc aatggcatcg
1501 taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc agaccgttca
1561 gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc
1621 ctttattcac attcttgccc gcctgatgaa tgctcatccg gaattccgta tggcaatgaa
1681 agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt tccatgagca
1741 aactgaaacg ttttcatcgc tctggagtga ataccacgac gatttccggc agtttctaca
1801 catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt
1861 tattgagaat atgtttttcg tctcagccaa tccctgggtg agtttcacca gttttgattt
1921 aaacgtggcc aatatggaca acttcttcgc ccccgttttc accatgggca aatattatac
1981 gcaaggcgac aaggtgctga tgccgctggc gattcaggtt catcatgccg tttgtgatgg
2041 cttccatgtc ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg
2101 ggcgtaaacg cgtggatccg gcttactaaa agccagataa cagtatgcgt atttgcgcgc
2161 tgatttttgc ggtataagaa tatatactga tatgtatacc cgaagtatgt caaaaagagg
2221 tatgctatga agcagcgtat tacagtgaca gttgacagcg acagctatca gttgctcaag
2281 gcatatatga tgtcaatatc tccggtctgg taagcacaac catgcagaat gaagcccgtc
2341 gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg gatggctgag gtcgcccggt
2401 ttattgaaat gaacggctct tttgctgacg agaacagggg ctggtgaaat gcagtttaag
2461 gtttacacct ataaaagaga gagccgttat cgtctgtttg tggatgtaca gagtgatatt
2521 attgacacgc ccgggcgacg gatggtgatc cccctggcca gtgcacgtct gctgtcagat
2581 aaagtctccc gtgaacttta cccggtggtg catatcgggg atgaaagctg gcgcatgatg
2641 accaccgata tggccagtgt gccggtctcc gttatcgggg aagaagtggc tgatctcagc
2701 caccgcgaaa atgacatcaa aaacgccatt aacctgatgt tctggggaat ataaatgtca
2761 ggctccctta tacacagcca gtctgcaggt cgaccatagt gactggatat gttgtgtttt
2821 acagtattat gtagtctgtt ttttatgcaa aatctaattt aatatattga tatttatatc
2881 attttacgtt tctcgttcag ctttcttgta caaagtggtg ataactctag agaattcact
2941 cctcaggtgc aggctgccta tcagaaggtg gtggctggtg tggccaatgc cctggctcac
3001 aaataccact gagatctttt tccctctgcc aaaaattatg gggacatcat gaagcccctt
3061 gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt gtgttggaat
3121 tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa acatcagaat
3181 gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat gaacaaaggt
3241 tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc cttattccat
3301 agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt atttttttct
3361 ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct cctctcctga
3421 ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgacct gcagcccaag
3481 cttggatccc tcgagttaat taaatctaga agtcgacagt actaagcttt gacagaaaag
3541 ccccatcctt aggcctcctc cttcctagtc tcctgatatt gggtctaacc cccacctcct
3601 gttaggcaga ttccttatct ggtgacacac ccccatttcc tggagccatc tctctccttg
3661 ccagaacctc taaggtttgc ttacgatgga gccagagagg atcctgggag ggagagcttg
3721 gcagggggtg ggagggaagg gggggatgcg tgacctgccc ggttctcagt ggccaccctg
3781 cgctaccctc tcccagaacc tgagctgctc tgacgcggct gtctggtgcg tttcactgat
3841 cctggtgctg cagcttcctt acacttccca agaggagaag cagtttggaa aaacaaaatc
3901 agaataagtt ggtcctgagt tctaactttg gctcttcacc tttctagtcc ccaatttata
3961 ttgttcctcc gtgcgtcagt tttacctgtg agataaggcc agtagccagc cccgtcctgg
4021 cagggctgtg gtgaggaggg gggtgtccgt gtggaaaact ccctttgtga gaatggtgcg
4081 tcctaggtgt tcaccaggtc gtggccgcct ctactccctt tctctttctc catccttctt
4141 tccttaaaga gtccccagtg ctatctggga catattcctc cgcccagagc agggtcccgc
4201 ttccctaagg ccctgctctg ggcttctggg tttgagtcct tggcaagccc aggagaggcg
4261 ctcaggcttc cctgtccccc ttcctcgtcc accatctcat gcccctggct ctcctgcccc
4321 ttccctacag gggttcctgg ctctgctcta gcgatcgcca attcgcccta tagtgagtcg
4381 tattacaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
4441 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
4501 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
4561 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
4621 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
4681 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
4741 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
4801 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
4861 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
4921 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
4981 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
5041 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
5101 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
5161 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
5221 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
5281 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
5341 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
5401 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
5461 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
5521 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
5581 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
5641 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
5701 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
5761 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
5821 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
5881 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
5941 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
6001 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
6061 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
6121 cgttccactg agcgtcaaac cccgtagaaa agatcaaagg atcttcttga gatccttttt
6181 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
6241 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
6301 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
6361 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
6421 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
6481 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
6541 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
6601 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
6661 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
6721 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
6781 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
6841 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
6901 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
6961 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
7021 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
7081 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
7141 caggaaacag ctatgaccat gattacgcca agctcgaaat taaccctcac taaagggaac
7201 aaaagctgtg ctttctctga ccagcattct ctcccctggg cctgtgccgc tttctgtctg
7261 cagcttgtgg cctgggtcac ctctacggct ggcccagatc cttccctgcc gcctccttca
7321 ggttccgtct tcctccactc cctcttcccc ttgctctctg ctgtgttgct gcccaaggat
7381 gctctttccg gagcacttcc ttctcggcgc tgcaccacgt gatgtcctct gagcggatcc
7441 tccccgtgtc tgggtcctct ccgggcatct ctcctccctc acccaacccc atgccgtctt
7501 cactcgctgg gttccctttt ccttctcctt ctggggcctg tgccatctct cgtttcttag
7561 gatggccttc tccgacggat gtctcccttg cgtcccgcct ccccttcttg taggcctgca
7621 tcatcaccgt ttttctggac aaccccaaag taccccgtct ccctggcttt agccacctct
7681 ccatcctctt gctttctttg cctggacacc ccgttctcct gtggattcgg gtcacctctc
7741 actcctttca tttgggcagc tcccctaccc cccttacctc tctagtctgt gctagctctt
7801 ccagccccct gtcatggcat cttccagggg tccgagagct cagctagtct tcttcctcca
7861 acccgggccc ctatgtccac ttcaggacag catgtttgct gcctccaggg atcctgtgtc
7921 cccgagctgg gaccacctta tattcccagg gccggttaat gtggctctgg ttctgggtac
7981 ttttatctgt cccctccacc ccacagtggg gcaagcttct gacctcttct cttcctccca
8041 cagggcctcg agagatctgg cagcggagag ggcagaggaa gtcttctaac atgcggtgac
8101 gtggaggaga atcccggccc taggctcgag atgaccgagt acaagcccac ggtgcgcctc
8161 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac
8221 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa
8281 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
8341 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
8401 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
8461 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
8521 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
8581 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
8641 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
8701 tggtgcatga cccgcaagcc cggtgcctga tctagagggc ccgtttaaac ccgctgatca
8761 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
8821 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
8881 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
8941 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggactagcgg
9001 tggcggcctc gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
9061 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
9121 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
9181 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
9241 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
9301 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
9361 atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact
9421 ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt
9481 tgtgcagcga tgggggcggg gg
//
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pAAVS1-P-CAG-DEST质粒载体应用举例
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