| 出品公司: | Origene |
|---|---|
| 载体名称: | pT7-Guide-IVT |
| 质粒类型: | 体外转录Crispr/Cas9载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | T7 |
| 载体大小: | 3.4 kb |
| 5’测序引物: | T7 |
| 3’测序引物: | M13-F |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 克隆菌株: | DH5 alpha; 等 |
| 产品目录号: | GE100025 |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
| 备注: |
pT7-Guide-IVT载体是T7启动子驱动的gRNA体外表达克隆载体
pT7-Guide-IVT载体在克隆靶序列后，可以通过体外转录产生gRNA
BsmB I位点用于pT7-Guide-IVT质粒靶序列克隆位点
对于通过显微注射或mRNA转染进行的mRNA介导的基因组编辑，需要体外表达Cas9 mRNA和gRNA转录本。T7驱动的体外表达Cas9 mRNA可以从单独的载体pT7-Cas9中产生
。
pT7-Guide-IVT质粒是体外表达gRNA，M13 Forward测序引物可用于克隆后对gRNA靶序列进行测序。 |
|
相关载体： |
pT7-Cas9 |
买家导航
pT7-Guide-IVT载体质粒基本信息
pT7-Guide-IVT质粒图谱载体图谱和pT7-Guide-IVT载体序列质粒序列多克隆位点信息
pT7-Guide-IVT质粒载体简介
pT7-Guide-IVT质粒序列载体序列
LOCUS pT7-Guide-IVT 3444 bp DNA circular SYN 07-MAR-2024
DEFINITION In vitro transcription vector with the T7 promoter, for producing a
guide RNA (gRNA).
ACCESSION .
VERSION .
KEYWORDS pT7-Guide-IVT
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3444)
AUTHORS OriGene
TITLE Direct Submission
JOURNAL Exported Jul 14, 2024 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..3444
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind complement(35..51)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
/note="color: #a020f0; direction: LEFT"
rep_origin 264..719
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
/note="color: #ffff00"
promoter 1001..1105
/gene="bla"
/label=AmpR promoter
/note="color: #ffffff; direction: RIGHT"
CDS 1106..1966
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/note="This feature has 2 segments:
1: 1106 .. 1174 / #ccffcc / signal sequence
2: 1175 .. 1966 / #ccffcc
Cleavage site after base 1174"
rep_origin 2137..2725
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
/note="color: #ffff00"
promoter 3228..3246
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
/note="color: #ffffff; direction: RIGHT"
misc_RNA 3300..3375
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
/note="color: #00ccff; direction: RIGHT"
ORIGIN
1 gaaagtcctc tccactgact gtagcctcca attcactggc cgtcgtttta caacgtcgtg
61 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
121 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
181 atggcgaatg gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
241 gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
301 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
361 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
421 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt
481 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
541 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
601 ctcgggctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
661 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt
721 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
781 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
841 agctgtgacc gtcaacggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
901 cgcgacccga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
961 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
1021 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
1081 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
1141 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
1201 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
1261 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
1321 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
1381 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
1441 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
1501 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
1561 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
1621 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
1681 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
1741 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
1801 atctggagcc ggtgagcgtg gttctcgcgg tatcattgca gcactggggc cagatggtaa
1861 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
1921 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
1981 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
2041 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
2101 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
2161 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
2221 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
2281 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
2341 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
2401 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
2461 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
2521 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
2581 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
2641 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
2701 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
2761 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
2821 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
2881 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
2941 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
3001 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
3061 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
3121 ctatgaccat gattacgcca agctccttcc tcttccagcc cttcctcttt cgggcggcag
3181 caagatccct tctgatctaa agtccaaccg gcgagagtga ggaattctaa tacgactcac
3241 tatagggaga cgtccactgg accaggcagc agcgtcagaa gacttttttg gaacgtctcg
3301 ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg
3361 gcaccgagtc ggtgctttta aaaagcttga attcgtgtcg catgaataac gggcagttat
3421 caaatgagca cggcgacagc ctgt
//
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pT7-Guide-IVT质粒载体应用举例
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