| 质粒名称: | pTAL2 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 31033 |
| 存储实验室: | Adam Bogdanove, Daniel Voytas |
|
载体骨架基本信息 |
|
| 载体名称: | pCR8-GW modified |
| 载体出品公司: | Invitrogen |
| 载体抗性: | 氨苄青霉素（50 ug/ml） |
| 空载体大小: | 2676 bp |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | TALE fusion |
| 物种来源: | Xanthamonas oryzae |
| 插入大小: | 2098 bp |
| 克隆方法: | 限制性内切酶 |
| 5' 测序引物 | TAL_F1 (ttggcgtcggcaaacagtgg) |
| 3' 测序引物 | TAL_R2 (ggcgacgaggtggtcgttgg) |
|
文献引用方法 |
|
| 材料和方法部分: | pTAL2 was a gift from Adam Bogdanove & Daniel Voytas (Addgene plasmid # 31033 ; http://n2t.net/addgene:31033 ; RRID:Addgene_31033) |
| 参考文献部分: | Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller JA, Somia NV, Bogdanove AJ, Voytas DF. Nucleic Acids Res. 2011 Apr 14. ():. 10.1093/nar/gkr218 PubMed 21493687 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pTAL2 4774 bp ds-DNA circular SYN 13-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pTAL2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4774)
AUTHORS Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller
JA, Somia NV, Bogdanove AJ, Voytas DF
TITLE Efficient design and assembly of custom TALEN and other TAL
effector-based constructs for DNA targeting.
JOURNAL Nucleic Acids Res. 2011 Apr 14. ():.
PUBMED 21493687
REFERENCE 2 (bases 1 to 4774)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..4774
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 1..17
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 33..132
/gene="mutant version of attL"
/label=attL1
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
CDS complement(927..1334)
/codon_start=1
/gene="lacZ fragment"
/product="LacZ-alpha fragment of beta-galactosidase"
/label=lacZ-alpha
/translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGARLQGG
TRQCIARCMHCLHCGHAATFCNLHKLACVQL"
primer_bind 1199..1215
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 1225..1243
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(1291..1311)
/label=T3
/note="T3 promoter, forward primer"
promoter complement(1291..1309)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(1330..1346)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(1343..1365)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 1354..1370
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(1378..1408)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 1423..1444
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
protein_bind complement(2282..2381)
/gene="mutant version of attL"
/label=attL2
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
promoter complement(2399..2417)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(2422..2438)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
CDS complement(2600..3460)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 3223..3242
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 3588..4176
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 4077..4096
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 4330..4347
/label=L4440
/note="L4440 vector, forward primer"
terminator 4506..4533
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
terminator 4625..4711
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
ORIGIN
1 gtaaaacgac ggccagtctt aagctcgggc cccaaataat gattttattt tgactgatag
61 tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca actttgtaca
121 aaaaagcagg ctccgaattc gcccttttgc atgtaaatag gaggtgcacc atggatccca
181 ttcgtccgcg caggccaagt cctgcccgcg agcttctgcc cggaccccaa ccggataggg
241 ttcagccgac tgcagatcgt ggggtgtctg cgcctgctgg cagccctctg gatggcttgc
301 ccgctcggcg gacggtgtcc cggacccggc tgccatctcc ccctgcgccc tcacctgcgt
361 tctcggcggg cagcttcagc gatctgctcc gtccgttcga tccgtcgctt cttgatacat
421 cgcttcttga ttcgatgcct gccgtcggca cgccgcatac agcggctgcc ccagcagagt
481 gggatgaggc gcaatcggct ctgcgtgcag ccgatgaccc gccacccacc gtgcgtgtcg
541 ctgtcactgc cgcgcggccg ccgcgcgcca agccggcccc gcgacggcgt gctgcgcaac
601 cctccgacgc ttcgccggcc gcgcaggtgg atctacgcac gctcggctac agtcagcagc
661 agcaagagaa gatcaaaccg aaggtgcgtt cgacagtggc gcagcaccac gaggcactgg
721 tgggccatgg gtttacacac gcgcacatcg ttgcgctcag ccaacacccg gcagcgttag
781 ggaccgtcgc tgtcacgtat cagcacataa tcacggcgtt gccagaggcg acacacgaag
841 acatcgttgg cgtcggcaaa cagtggtccg gcgcacgcgc cctggaggcc ttgctcacgg
901 atgcggggga gttgagaggt ccgccgttac agttggacac aggccaactt gtgaagattg
961 caaaacgtgg cggcgtgacc gcaatggagg cagtgcatgc atcgcgcaat gcactgacgg
1021 gtgcccccct ggagacgggc gccgctacag ggcgcgtccc attcgccatt caggctgcgc
1081 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
1141 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
1201 aaaacgacgg ccagtgagcg cgcgtaatac gactcactat agggcgaatt gggtaccggg
1261 ccccccctcg aggtcctcca gcttttgttc cctttagtga gggttaattg cgcgcttggc
1321 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
1381 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
1441 attaattgcg ttgcgctcac tgcccgcttt ccaccggtcg tctccaacga ccacctcgtc
1501 gccttggcct gcctcggcgg acgtcctgcc atggatgcag tgaaaaaggg attgccgcac
1561 gcgccggaat tgatcagaag agtcaatcgc cgtattggcg aacgcacgtc ccatcgcgtt
1621 gccgactacg cgcaagtggt tcgcgtgctg gagtttttcc agtgccactc ccacccagcg
1681 tacgcatttg atgaggccat gacgcagttc gggatgagca ggaacgggtt ggtacagctc
1741 tttcgcagag tgggcgtcac cgaactcgaa gcccgcggtg gaacgctccc cccagcctcg
1801 cagcgttggg accgtatcct ccaggcatca gggatgaaaa gggccaaacc gtcccctact
1861 tcagctcaaa caccggatca ggcgtctttg catgcattcg ccgattcgct ggagcgtgac
1921 cttgatgcgc ccagcccaat gcacgaggga gatcagacgc gggcaagcag ccgtaaacgg
1981 tcccgatcgg atcgtgctgt caccggcccc tccgcacagc aggctgtcga ggtgcgcgtt
2041 cccgaacagc gcgatgcgct gcatttgccc ctcagctgga gggtaaaacg cccgcgtacc
2101 aggatctggg gcggcctccc ggatcctggt acgcccatgg ctgccgacct ggcagcgtcc
2161 agcaccgtga tgtgggaaca agatgcggac cccttcgcag gggcagcgga tgatttcccg
2221 gcattcaacg aagaggaact cgcatggttg atggagctat tgcctcagaa gggcgaattc
2281 gacccagctt tcttgtacaa agttggcatt ataaaaaata attgctcatc aatttgttgc
2341 aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc tgatatcccc
2401 tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa
2461 aatctctgat gttacattgc acaagataaa aatatatcat catgagatta tcaaaaagga
2521 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
2581 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
2641 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
2701 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
2761 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
2821 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
2881 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
2941 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
3001 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
3061 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
3121 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
3181 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
3241 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
3301 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
3361 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
3421 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
3481 attgaagcat ttatcagggt tattgtctca tgaccaaaat cccttaacgt gagttacgcg
3541 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
3601 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
3661 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
3721 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
3781 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
3841 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
3901 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
3961 agatacctac agcgtgagca ttgagaaagc gccacgcttc ccgaagggag aaaggcggac
4021 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
4081 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
4141 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
4201 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
4261 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
4321 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
4381 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
4441 gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa
4501 acgcaaaaag gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg
4561 gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg
4621 gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc
4681 agtcttccga ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcgtta
4741 acgctagcat ggatgttttc ccagtcacga cgtt
//
