pCambia1300-CFP

LOCUS       pCambia1300-CFP               11189 bp DNA     circular SYN 12-JUN-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11189)
  AUTHORS   Ming
  TITLE     Direct Submission
  JOURNAL   Exported Jun 12, 2025 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..11189
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1..17)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     misc_feature    220..244
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
                     /note="color: #ffe4c4"
     CDS             1544..2173
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /note="color: #993366"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2607..3674
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /note="color: #993366"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      3740..3934
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /note="color: #ffff00"
     misc_feature    4278..4418
                     /label=bom
                     /note="basis of mobility region from pBR322"
                     /note="color: #a6acb3"
     rep_origin      complement(4604..5192)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(5279..6073)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /note="color: #ccffcc"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    6498..6522
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
                     /note="color: #ffe4c4"
     polyA_signal    6600..6774
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
                     /note="color: #a6acb3"
     CDS             complement(6814..7839)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /note="color: #ccffcc"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(7907..8584)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
                     /note="color: #ffffff; direction: LEFT"
     protein_bind    8775..8796
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     promoter        8811..8841
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This forward directional feature has 3 segments:
                        1: 8811 .. 8816 / #ffffff / -35
                        2: 8817 .. 8834 / #ffffff
                        3: 8835 .. 8841 / #ffffff / -10"
     protein_bind    8849..8865
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     8873..8889
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     promoter        9398..9743
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             9802..10521
                     /codon_start=1
                     /product="cyan variant of GFP"
                     /label=CFP
                     /note="mammalian codon-optimized"
                     /note="This feature has 3 segments:
                        1:  9802 ..  9804 / #00ffff
                        2:  9805 ..  9807 / #00ffff / 1a
                        3:  9808 .. 10521 / #00ffff"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     terminator      10541..11182
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
                     /note="color: #ffffff; direction: RIGHT"
ORIGIN
        1 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
       61 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
      121 cccttcccaa cagttgcgca gcctgaatgg cgaatgctag agcagcttga gcttggatca
      181 gattgtcgtt tcccgccttc agtttaaact atcagtgttt gacaggatat attggcgggt
      241 aaacctaaga gaaaagagcg tttattagaa taacggatat ttaaaagggc gtgaaaaggt
      301 ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttcccctcg ggatcaaagt
      361 actttgatcc aacccctccg ctgctatagt gcagtcggct tctgacgttc agtgcagccg
      421 tcttctgaaa acgacatgtc gcacaagtcc taagttacgc gacaggctgc cgccctgccc
      481 ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa agtagaatac ttgcgactag
      541 aaccggagac attacgccat gaacaagagc gccgccgctg gcctgctggg ctatgcccgc
      601 gtcagcaccg acgaccagga cttgaccaac caacgggccg aactgcacgc ggccggctgc
      661 accaagctgt tttccgagaa gatcaccggc accaggcgcg accgcccgga gctggccagg
      721 atgcttgacc acctacgccc tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc
      781 cgcagcaccc gcgacctact ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg
      841 cgtagcctgg cagagccgtg ggccgacacc accacgccgg ccggccgcat ggtgttgacc
      901 gtgttcgccg gcattgccga gttcgagcgt tccctaatca tcgaccgcac ccggagcggg
      961 cgcgaggccg ccaaggcccg aggcgtgaag tttggccccc gccctaccct caccccggca
     1021 cagatcgcgc acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa agaggcggct
     1081 gcactgcttg gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg
     1141 acgcccaccg aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac
     1201 gccctggcgg ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg caccaggacg
     1261 gccaggacga accgtttttc attaccgaag agatcgaggc ggagatgatc gcggccgggt
     1321 acgtgttcga gccgcccgcg cacgtctcaa ccgtgcggct gcatgaaatc ctggccggtt
     1381 tgtctgatgc caagctggcg gcctggccgg ccagcttggc cgctgaagaa accgagcgcc
     1441 gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt
     1501 atatgatgcg atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt
     1561 acttaaccag aaaggcgggt caggcaagac gaccatcgca acccatctag cccgcgccct
     1621 gcaactcgcc ggggccgatg ttctgttagt cgattccgat ccccagggca gtgcccgcga
     1681 ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat
     1741 tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg gagcgcccca
     1801 ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca
     1861 gccaagccct tacgacatat gggccaccgc cgacctggtg gagctggtta agcagcgcat
     1921 tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac
     1981 gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac gagctgccca ttcttgagtc
     2041 ccgtatcacg cagcgcgtga gctacccagg cactgccgcc gccggcacaa ccgttcttga
     2101 atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc
     2161 aaaactcatt tgagttaatg aggtaaagag aaaatgagca aaagcacaaa cacgctaagt
     2221 gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt tggccagcct ggcagacacg
     2281 ccagccatga agcgggtcaa ctttcagttg ccggcggagg atcacaccaa gctgaagatg
     2341 tacgcggtac gccaaggcaa gaccattacc gagctgctat ctgaatacat cgcgcagcta
     2401 ccagagtaaa tgagcaaatg aataaatgag tagatgaatt ttagcggcta aaggaggcgg
     2461 catggaaaat caagaacaac caggcaccga cgccgtggaa tgccccatgt gtggaggaac
     2521 gggcggttgg ccaggcgtaa gcggctgggt tgtctgccgg ccctgcaatg gcactggaac
     2581 ccccaagccc gaggaatcgg cgtgacggtc gcaaaccatc cggcccggta caaatcggcg
     2641 cggcgctggg tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac
     2701 gcatcgaggc agaagcacgc cccggtgaat cgtggcaagc ggccgctgat cgaatccgca
     2761 aagaatcccg gcaaccgccg gcagccggtg cgccgtcgat taggaagccg cccaagggcg
     2821 acgagcaacc agattttttc gttccgatgc tctatgacgt gggcacccgc gatagtcgca
     2881 gcatcatgga cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga
     2941 tccgctacga gcttccagac gggcacgtag aggtttccgc agggccggcc ggcatggcca
     3001 gtgtgtggga ttacgacctg gtactgatgg cggtttccca tctaaccgaa tccatgaacc
     3061 gataccggga agggaaggga gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg
     3121 tactcaagtt ctgccggcga gccgatggcg gaaagcagaa agacgacctg gtagaaacct
     3181 gcattcggtt aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc
     3241 gcctggtgac ggtatccgag ggtgaagcct tgattagccg ctacaagatc gtaaagagcg
     3301 aaaccgggcg gccggagtac atcgagatcg agctagctga ttggatgtac cgcgagatca
     3361 cagaaggcaa gaacccggac gtgctgacgg ttcaccccga ttactttttg atcgatcccg
     3421 gcatcggccg ttttctctac cgcctggcac gccgcgccgc aggcaaggca gaagccagat
     3481 ggttgttcaa gacgatctac gaacgcagtg gcagcgccgg agagttcaag aagttctgtt
     3541 tcaccgtgcg caagctgatc gggtcaaatg acctgccgga gtacgatttg aaggaggagg
     3601 cggggcaggc tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat
     3661 ccgccggttc ctaatgtacg gagcagatgc tagggcaaat tgccctagca ggggaaaaag
     3721 gtcgaaaagg tctctttcct gtggatagca cgtacattgg gaacccaaag ccgtacattg
     3781 ggaaccggaa cccgtacatt gggaacccaa agccgtacat tgggaaccgg tcacacatgt
     3841 aagtgactga tataaaagag aaaaaaggcg atttttccgc ctaaaactct ttaaaactta
     3901 ttaaaactct taaaacccgc ctggcctgtg cataactgtc tggccagcgc acagccgaag
     3961 agctgcaaaa agcgcctacc cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc
     4021 ggcctatcgc ggccgctggc cgctcaaaaa tggctggcct acggccaggc aatctaccag
     4081 ggcgcggaca agccgcgccg tcgccactcg accgccggcg cccacatcaa ggcaccctgc
     4141 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     4201 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     4261 gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact
     4321 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
     4381 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca
     4441 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     4501 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     4561 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     4621 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     4681 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     4741 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     4801 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     4861 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     4921 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     4981 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     5041 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     5101 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     5161 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     5221 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgcat tctaggtact
     5281 aaaacaattc atccagtaaa atataatatt ttattttctc ccaatcaggc ttgatcccca
     5341 gtaagtcaaa aaatagctcg acatactgtt cttccccgat atcctccctg atcgaccgga
     5401 cgcagaaggc aatgtcatac cacttgtccg ccctgccgct tctcccaaga tcaataaagc
     5461 cacttacttt gccatctttc acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga
     5521 caagttcctc ttcgggcttt tccgtcttta aaaaatcata cagctcgcgc ggatctttaa
     5581 atggagtgtc ttcttcccag ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt
     5641 aatccaattc ggctaagcgg ctgtctaagc tattcgtata gggacaatcc gatatgtcga
     5701 tggagtgaaa gagcctgatg cactccgcat acagctcgat aatcttttca gggctttgtt
     5761 catcttcata ctcttccgag caaaggacgc catcggcctc actcatgagc agattgctcc
     5821 agccatcatg ccgttcaaag tgcaggacct ttggaacagg cagctttcct tccagccata
     5881 gcatcatgtc cttttcccgt tccacatcat aggtggtccc tttataccgg ctgtccgtca
     5941 tttttaaata taggttttca ttttctccca ccagcttata taccttagca ggagacattc
     6001 cttccgtatc ttttacgcag cggtattttt cgatcagttt tttcaattcc ggtgatattc
     6061 tcattttagc catttattat ttccttcctc ttttctacag tatttaaaga taccccaaga
     6121 agctaattat aacaagacga actccaattc actgttcctt gcattctaaa accttaaata
     6181 ccagaaaaca gctttttcaa agttgttttc aaagttggcg tataacatag tatcgacgga
     6241 gccgattttg aaaccgcggt gatcacaggc agcaacgctc tgtcatcgtt acaatcaaca
     6301 tgctaccctc cgcgagatca tccgtgtttc aaacccggca gcttagttgc cgttcttccg
     6361 aatagcatcg gtaacatgag caaagtctgc cgccttacaa cggctctccc gctgacgccg
     6421 tcccggactg atgggctgcc tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg
     6481 agctgttggc tggctggtgg caggatatat tgtggtgtaa acaaattgac gcttagacaa
     6541 cttaataaca cattgcggac gtttttaatg tactgaatta acgccgaatt aattcggggg
     6601 atctggattt tagtactgga ttttggtttt aggaattaga aattttattg atagaagtat
     6661 tttacaaata caaatacata ctaagggttt cttatatgct caacacatga gcgaaaccct
     6721 ataggaaccc taattccctt atctgggaac tactcacaca ttattatgga gaaactcgag
     6781 cttgtcgatc gacagatccg gtcggcatct actctatttc tttgccctcg gacgagtgct
     6841 ggggcgtcgg tttccactat cggcgagtac ttctacacag ccatcggtcc agacggccgc
     6901 gcttctgcgg gcgatttgtg tacgcccgac agtcccggct ccggatcgga cgattgcgtc
     6961 gcatcgaccc tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc tctgatagag
     7021 ttggtcaaga ccaatgcgga gcatatacgc ccggagtcgt ggcgatcctg caagctccgg
     7081 atgcctccgc tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg gcctccagaa
     7141 gaagatgttg gcgacctcgt attgggaatc cccgaacatc gcctcgctcc agtcaatgac
     7201 cgctgttatg cggccattgt ccgtcaggac attgttggag ccgaaatccg cgtgcacgag
     7261 gtgccggact tcggggcagt cctcggccca aagcatcagc tcatcgagag cctgcgcgac
     7321 ggacgcactg acggtgtcgt ccatcacagt ttgccagtga tacacatggg gatcagcaat
     7381 cgcgcatatg aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc cgaatgggcc
     7441 gaacccgctc gtctggctaa gatcggccgc agcgatcgca tccatagcct ccgcgaccgg
     7501 ttgtagaaca gcgggcagtt cggtttcagg caggtcttgc aacgtgacac cctgtgcacg
     7561 gcgggagatg caataggtca ggctctcgct aaactcccca atgtcaagca cttccggaat
     7621 cgggagcgcg gccgatgcaa agtgccgata aacataacga tctttgtaga aaccatcggc
     7681 gcagctattt acccgcagga catatccacg ccctcctaca tcgaagctga aagcacgaga
     7741 ttcttcgccc tccgagagct gcatcaggtc ggagacgctg tcgaactttt cgatcagaaa
     7801 cttctcgaca gacgtcgcgg tgagttcagg ctttttcata tctcattgcc ccccgggatc
     7861 tgcgaaagct cgagagagat agatttgtag agagagactg gtgatttcag cgtgtcctct
     7921 ccaaatgaaa tgaacttcct tatatagagg aaggtcttgc gaaggatagt gggattgtgc
     7981 gtcatccctt acgtcagtgg agatatcaca tcaatccact tgctttgaag acgtggttgg
     8041 aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg gaccactgtc
     8101 ggcagaggca tcttgaacga tagcctttcc tttatcgcaa tgatggcatt tgtaggtgcc
     8161 accttccttt tctactgtcc ttttgatgaa gtgacagata gctgggcaat ggaatccgag
     8221 gaggtttccc gatattaccc tttgttgaaa agtctcaata gccctttggt cttctgagac
     8281 tgtatctttg atattcttgg agtagacgag agtgtcgtgc tccaccatgt tatcacatca
     8341 atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg
     8401 tgggggtcca tctttgggac cactgtcggc agaggcatct tgaacgatag cctttccttt
     8461 atcgcaatga tggcatttgt aggtgccacc ttccttttct actgtccttt tgatgaagtg
     8521 acagatagct gggcaatgga atccgaggag gtttcccgat attacccttt gttgaaaagt
     8581 ctcaatagcc ctttggtctt ctgagactgt atctttgata ttcttggagt agacgagagt
     8641 gtcgtgctcc accatgttgg caagctgctc tagccaatac gcaaaccgcc tctccccgcg
     8701 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     8761 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     8821 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     8881 agctatgaca tgattacgaa ttcggtcccc agattagcct tttcaatttc agaaagaatg
     8941 ctaacccaca gatggttaga gaggcttacg cagcaggtct catcaagacg atctacccga
     9001 gcaataatct ccaggaaatc aaataccttc ccaagaaggt taaagatgca gtcaaaagat
     9061 tcaggactaa ctgcatcaag aacacagaga aagatatatt tctcaagatc agaagtacta
     9121 ttccagtatg gacgattcaa ggcttgcttc acaaaccaag gcaagtaata gagattggag
     9181 tctctaaaaa ggtagttccc actgaatcaa aggccatgga gtcaaagatt caaatagagg
     9241 acctaacaga actcgccgta aagactggcg aacagttcat acagagtctc ttacgactca
     9301 atgacaagaa gaaaatcttc gtcaacatgg tggagcacga cacacttgtc tactccaaaa
     9361 atatcaaaga tacagtctca gaagaccaaa gggcaattga gacttttcaa caaagggtaa
     9421 tatccggaaa cctcctcgga ttccattgcc cagctatctg tcactttatt gtgaagatag
     9481 tggaaaagga aggtggctcc tacaaatgcc atcattgcga taaaggaaag gccatcgttg
     9541 aagatgcctc tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg
     9601 aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat atctccactg
     9661 acgtaaggga tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa
     9721 gttcatttca tttggagaga acacggggga cgagctcaag cttcgaattc tgcagtcgac
     9781 ggtaccgcgg gcccgggatc catggtgagc aagggcgagg agctgttcac cggggtggtg
     9841 cccatcctgg tcgagctgga cggcgacgta aacggccaca ggttcagcgt gtccggcgag
     9901 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     9961 ctgcccgtgc cctggcccac cctcgtgacc accctgacct ggggcgtgca gtgcttcagc
    10021 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
    10081 gtccaggagc gtaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
    10141 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
    10201 gacggcaaca tcctggggca caagctggag tacaactaca tcagccacaa cgtctatatc
    10261 accgccgaca agcagaagaa cggcatcaag gcccacttca agatccgcca caacatcgag
    10321 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
    10381 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
    10441 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
    10501 atggacgagc tgtacaagtg atctaggggc tagaatgcag agctttcgtt cgtatcatcg
    10561 gtttcgacaa cgttcgtcaa gttcaatgca tcagtttcat tgcgcacaca ccagaatcct
    10621 actgagtttg agtattatgg cattgggaaa actgtttttc ttgtaccatt tgttgtgctt
    10681 gtaatttact gtgtttttta ttcggttttc gctatcgaac tgtgaaatgg aaatggatgg
    10741 agaagagtta atgaatgata tggtcctttt gttcattctc aaattaatat tatttgtttt
    10801 ttctcttatt tgttgtgtgt tgaatttgaa attataagag atatgcaaac attttgtttt
    10861 gagtaaaaat gtgtcaaatc gtggcctcta atgaccgaag ttaatatgag gagtaaaaca
    10921 cttgtagttg taccattatg cttattcact aggcaacaaa tatattttca gacctagaaa
    10981 agctgcaaat gttactgaat acaagtatgt cctcttgtgt tttagacatt tatgaacttt
    11041 cctttatgta attttccaga atccttgtca gattctaatc attgctttat aattatagtt
    11101 atactcatgg atttgtagtt gagtatgaaa atatttttta atgcatttta tgacttgcca
    11161 attgattgac aacatgcatc aagcttggc
//