| 质粒名称: | FUW-tetO-lox-hSOX2 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 20729 |
| 存储实验室: | Rudolf Jaenisch |
|
载体骨架基本信息 |
|
| 载体名称: | FUW |
| 载体出品公司: | Carlos Lois |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 6454 bp |
| 完整质粒大小: | 7420 bp |
| 载体类型: | 哺乳细胞表达，Lentiviral |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | SOX2 |
| 物种来源: | 人源 |
| 插入大小: | 966 bp |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | EcoRI(未破坏) |
| 3' 克隆位点: | EcoRI(未破坏) |
| 5' 测序引物 | LNCX |
|
文献引用方法 |
|
| 材料和方法部分: | FUW-tetO-lox-hSOX2 was a gift from Rudolf Jaenisch (爱迪基因 plasmid # 20729) |
| 参考文献部分: | Parkinson's Disease Patient-Derived Induced Pluripotent Stem Cells Free of Viral Reprogramming Factors. Frank Soldner, Dirk Hockemeyer, Caroline Beard, Qing Gao, George W. Bell, Elizabeth G. Cook, Gunnar Hargus, Alexandra Blak, Oliver Cooper, Maisam Mitalipova, Ole Isacson, Rudolf Jaenisch. Cell, Volume 136, Issue 5, 964-977, 6 March 2009 10.1016/j.cell.2009.02.013 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS FUW-tetO-lox-hSOX2 7420 bp ds-DNA circular SYN 10-JAN-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS FUW-tetO-lox-hSOX2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7420)
AUTHORS Frank Soldner, Dirk Hockemeyer, Caroline Beard, Qing Gao, George W.
Bell, Elizabeth G. Cook, Gunnar Hargus, Alexandra Blak, Oliver
Cooper, Maisam Mitalipova, Ole Isacson, Rudolf Jaenisch
TITLE Parkinson's Disease Patient-Derived Induced Pluripotent Stem Cells
Free of Viral Reprogramming Factors
JOURNAL Cell, Volume 136, Issue 5, 964-977, 6 March 2009
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|FUW-tetO-lox-hSOX2|
FEATURES Location/Qualifiers
source 1..7420
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 7..386
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 388..586
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 541..561
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 604..784
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 831..956
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1449..1682
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 2209..2326
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
protein_bind 2385..2655
/label=tetracycline response element
/bound_moiety="tetracycline repressor TetR"
/note="contains seven copies of the tetracycline operator
tetO"
promoter 2688..2725
/label=minimal CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 2722..2746
/label=LNCX
/note="Human CMV promoter, forward primer"
misc_feature 3809..4397
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(3862..3882)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(4280..4291)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(4400..4416)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4401..4417)
/label=pBluescriptKS
/note="For pBluescript vector"
protein_bind complement(4921..4954)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
LTR 4968..5148
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
primer_bind complement(5174..5191)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 5180..5291
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin complement(5437..6025)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5517..5536)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(6196..7056)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6819..6838
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7057..7161)
/gene="bla"
/label=AmpR promoter
primer_bind complement(7236..7255)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
241 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
301 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
601 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
661 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
721 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
781 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
841 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
901 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
961 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
1021 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
1081 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
1141 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
1201 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
1261 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
1321 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
1381 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
1441 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
1501 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
1561 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
1621 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
1681 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
1741 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
1801 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
1861 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
1921 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
1981 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
2041 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
2101 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg
2161 gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa
2221 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
2281 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
2341 ggacagcaga gatccagttt ggttaatgat ctcgagttta ccactcccta tcagtgatag
2401 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
2461 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
2521 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
2581 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
2641 tatcagtgat agagaaaagt gaaagtcgag ctcggtaccc gggtcgagta ggcgtgtacg
2701 gtgggaggcc tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagacgcca
2761 tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcctcc gcggccccga
2821 attcgccacc atgtacaaca tgatggagac ggagctgaag ccgccgggcc cgcagcaaac
2881 ttcggggggc ggcggcggca actccaccgc ggcggcggcc ggcggcaacc agaaaaacag
2941 cccggaccgc gtcaagcggc ccatgaatgc cttcatggtg tggtcccgcg ggcagcggcg
3001 caagatggcc caggagaacc ccaagatgca caactcggag atcagcaagc gcctgggcgc
3061 cgagtggaaa cttttgtcgg agacggagaa gcggccgttc atcgacgagg ctaagcggct
3121 gcgagcgctg cacatgaagg agcacccgga ttataaatac cggccccggc ggaaaaccaa
3181 gacgctcatg aagaaggata agtacacgct gcccggcggg ctgctggccc ccggcggcaa
3241 tagcatggcg agcggggtcg gggtgggcgc cggcctgggc gcgggcgtga accagcgcat
3301 ggacagttac gcgcacatga acggctggag caacggcagc tacagcatga tgcaggacca
3361 gctgggctac ccgcagcacc cgggcctcaa tgcgcacggc gcagcgcaga tgcagcccat
3421 gcaccgctac gacgtgagcg ccctgcagta caactccatg accagctcgc agacctacat
3481 gaacggctcg cccacctaca gcatgtccta ctcgcagcag ggcacccctg gcatggctct
3541 tggctccatg ggttcggtgg tcaagtccga ggccagctcc agcccccctg tggttacctc
3601 ttcctcccac tccagggcgc cctgccaggc cggggacctc cgggacatga tcagcatgta
3661 tctccccggc gccgaggtgc cggaacccgc cgcccccagc agacttcaca tgtcccagca
3721 ctaccagagc ggcccggtgc ccggcacggc cattaacggc acactgcccc tctcacacat
3781 gtgagaattc gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag
3841 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
3901 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
3961 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
4021 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
4081 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
4141 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
4201 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
4261 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
4321 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
4381 ttgggccgcc tccccgcatc gataccgtcg acctcgagac ctagaaaaac atggagcaat
4441 cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga
4501 ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc
4561 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
4621 ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga
4681 ttggcagaac tacacaccag ggccagggat cagatatcca ctgacctttg gatggtgcta
4741 caagctagta ccagttgagc aagagaaggt agaagaagcc aatgaaggag agaacacccg
4801 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tattagagtg
4861 gaggtttgac agccgcctag catttcatca catggcccga gagctgcatc cggactcgag
4921 ataacttcgt ataatgtatg ctatacgaag ttattccgga ctgtactggg tctctctggt
4981 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
5041 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
5101 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa
5161 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
5221 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
5281 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
5341 gacagcaagg gggaggattg ggaagacaat agcaggcatg tgagcaaaag gccagcaaaa
5401 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
5461 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
5521 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
5581 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
5641 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
5701 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
5761 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
5821 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
5881 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
5941 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
6001 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
6061 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
6121 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
6181 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
6241 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
6301 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
6361 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
6421 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
6481 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
6541 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
6601 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
6661 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
6721 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
6781 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
6841 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
6901 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
6961 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
7021 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
7081 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
7141 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
7201 gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag
7261 ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa
7321 atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt
7381 aggcgttttg cgctgcttcg cgatgtacgg gccagatata
//
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