| 质粒名称: | pCDH-EFIa-Id1-PGK-puro |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 107735 |
| 存储实验室: | Alexandre Colas |
|
载体骨架基本信息 |
|
| 载体名称: | pCDH-EF1mcs-PGKpuro |
| 载体出品公司: | System Biosciences |
| 空载体大小: | 8650 bp |
| 完整质粒大小: | 9589 bp |
| 载体类型: | Lentiviral |
| 筛选标记: | Puromycin |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Id1 |
| 物种来源: | M. musculus (mouse) |
| 插入大小: | 939 bp |
| 基因突变: | Used pCDH-CMV-MCS-copGFP stock plasmid CD511B-1 from System Biosciences. Modified by replacing CMV promoter with EF1 and replacing copGFP with PGKpuro |
| GenBank编号 | NM_010495 NM_010495 |
| 启动子: | EF1a |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | XbaI (unknown if destroyed) |
| 3’克隆位点: | EcoRI (unknown if destroyed) |
| 5’测序引物 | ATGAAGGTCGCCAGTGGCAGTGCC |
| 3’测序引物 | TCAGCGACACAAGATGCGATCGTC |
|
文献引用方法 |
|
| 材料和方法部分: | pCDH-EFIa-Id1-PGK-puro was a gift from Alexandre Colas (Addgene plasmid # 107735 ; http://n2t.net/addgene:107735 ; RRID:Addgene_107735) |
| 参考文献部分: | Id genes are essential for early heart formation. Cunningham TJ, Yu MS, McKeithan WL, Spiering S, Carrette F, Huang CT, Bushway PJ, Tierney M, Albini S, Giacca M, Mano M, Puri PL, Sacco A, Ruiz-Lozano P, Riou JF, Umbhauer M, Duester G, Mercola M, Colas AR. Genes Dev. 2017 Aug 9. pii: gad.300400.117. doi: 10.1101/gad.300400.117. 10.1101/gad.300400.117 PubMed 28794185 |
买家导航
pCDH-EFIa-Id1-PGK-puro载体质粒基本信息
pCDH-EFIa-Id1-PGK-puro质粒图谱载体图谱和pCDH-EFIa-Id1-PGK-puro载体序列质粒序列多克隆位点信息
pCDH-EFIa-Id1-PGK-puro质粒载体简介
pCDH-EFIa-Id1-PGK-puro质粒序列载体序列
LOCUS pCDH-EFIa-Id1-PGK-puro 9171 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Expresses Id1 in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS pCDH-EFIa-Id1-PGK-puro
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9171)
AUTHORS Cunningham TJ, Yu MS, McKeithan WL, Spiering S, Carrette F, Huang
CT, Bushway PJ, Tierney M, Albini S, Giacca M, Mano M, Puri PL,
Sacco A, Ruiz-Lozano P, Riou JF, Umbhauer M, Duester G, Mercola M,
Colas AR
TITLE Id genes are essential for early heart formation.
JOURNAL Genes Dev. 2017 Aug 9. pii: gad.300400.117. doi:
10.1101/gad.300400.117.
PUBMED 28794185
REFERENCE 2 (bases 1 to 9171)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..9171
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 2..24
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 16..33
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 17..33
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 48..274
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 275..455
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 502..627
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1120..1353
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1538..1582
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 1849..1965
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2014..2225
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 2238..2506
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
promoter 2577..2788
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 2801..3069
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
promoter 3140..3351
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 3364..3632
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
promoter 4154..4664
/label=hPGK promoter
/note="human phosphoglycerate kinase 1 promoter"
CDS 4686..5285
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(4686..4705)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 5182..5202
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
misc_feature 5292..5880
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(5345..5365)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(5763..5774)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 5954..6187
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 6259..6380
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(6296..6315)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 6350..6369
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 6420..6555
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 6482..6501
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(6588..6604)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6588..6604)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(6601..6623)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 6612..6628
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6636..6666)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6681..6702
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(6819..6836)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6990..7578)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(7070..7089)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7749..8609)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8372..8391
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8610..8714)
/gene="bla"
/label=AmpR promoter
primer_bind 8782..8800
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(8838..8860)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 8960..8979
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tgacgcgtgt agtcttatgc
61 aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga
121 gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg
181 aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga
241 tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg
301 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
361 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
421 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
481 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
541 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
601 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
661 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
721 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
781 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
841 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
901 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
961 caagcggcca ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
1021 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
1081 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
1141 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
1201 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
1261 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
1321 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
1381 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
1441 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
1501 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
1561 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
1621 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
1681 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
1741 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
1801 agacagatcc attcgattag tgaacggatc tcgacggtat cggttaactt ttaaaagaaa
1861 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
1921 acaaactaaa gaattacaaa aacaaattac aaaattcaaa attttatcga tactagtccg
1981 caaggatctg cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc
2041 ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg
2101 taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac
2161 cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa
2221 cacagctgaa gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc cctacctgag
2281 gccgccatcc acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa
2341 ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag accgggcctt tgtccggcgc
2401 tcccttggag cctacctaga ctcagccggc tctccacgct ttgcctgacc ctgcttgctc
2461 aactctacgt ctttgtttcg ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg
2521 cgcctacgct agactctagt ccgcaaggat ctgcgatcgc tccggtgccc gtcagtgggc
2581 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaacggg
2641 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
2701 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
2761 tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc
2821 acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc
2881 tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc
2941 gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac
3001 gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg
3061 ttacagatcc aagctgtgac cggcgcctac gctagactct agtccgcaag gatctgcgat
3121 cgctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
3181 ggaggggtcg gcaattgaac gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
3241 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
3301 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca gctgaagctt
3361 cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc
3421 cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc
3481 taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc ttggagccta
3541 cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt
3601 gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc tacgctagac
3661 tctagagcca ccatgaaggt cgccagtggc agtgccgcag ccgctgcagg ccctagctgt
3721 tcgctgaagg cgggcaggac agcgggcgag gtggtacttg gtctgtcgga gcaaagcgtg
3781 gccatctcgc gctgcgctgg gacgcgcctg cccgccttgc tggacgagca gcaggtgaac
3841 gtcctgctct acgacatgaa cggctgctac tcacgcctca aggagctggt gcccaccctg
3901 ccccagaacc gcaaagtgag caaggtggag atcctgcagc atgtaatcga ctacatcagg
3961 gacctgcagc tggagctgaa ctcggagtct gaagtcggga ccaccggagg ccggggactg
4021 cctgtccgcg ccccgctcag caccctgaac ggcgagatca gtgccttggc ggccgaggcg
4081 gcatgtgttc cagccgacga tcgcatcttg tgtcgctgag aattcgaatt taaatcggat
4141 ccgcggccgc cacggggttg gggttgcgcc ttttccaagg cagccctggg tttgcgcagg
4201 gacgcggctg ctctgggcgt ggttccggga aacgcagcgg cgccgaccct gggtctcgca
4261 cattcttcac gtccgttcgc agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc
4321 cggcgacgct tcctgctccg cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc
4381 ggacgtgaca aacggaagcc gcacgtctca ctagtaccct cgcagacgga cagcgccagg
4441 gagcaatggc agcgcgccga ccgcgatggg ctgtggccaa tagcggctgc tcagcagggc
4501 gcgccgagag cagcggccgg gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt
4561 gggccctgtt cctgcccgcg cggtgttccg cactctgcaa gcctccggag cgcacgtcgg
4621 cagtcggctc cctcgttgac cgaatcaccg acctctctcc ccaggggaat ccaccggacg
4681 ccaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg
4741 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgttgacc
4801 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
4861 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
4921 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
4981 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
5041 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
5101 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
5161 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
5221 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
5281 cctgagtcga caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
5341 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
5401 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
5461 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
5521 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
5581 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
5641 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
5701 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
5761 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
5821 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
5881 ctggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
5941 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gataagatct gctttttgct
6001 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
6061 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
6121 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
6181 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
6241 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
6301 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
6361 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
6421 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
6481 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
6541 gcttttttgg aggcctagac ttttgcagag accaaattcg taatcatgtc atagctgttt
6601 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
6661 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
6721 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
6781 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
6841 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
6901 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
6961 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
7021 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
7081 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
7141 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
7201 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
7261 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
7321 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
7381 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
7441 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
7501 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
7561 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
7621 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
7681 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
7741 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
7801 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
7861 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
7921 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
7981 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
8041 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
8101 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
8161 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
8221 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
8281 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
8341 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
8401 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
8461 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
8521 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
8581 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
8641 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
8701 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
8761 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc
8821 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
8881 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
8941 cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
9001 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc
9061 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
9121 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt t
//
