| 质粒名称: | pPD95_67 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 1490 |
| 存储实验室: | Andrew Fire |
|
载体骨架基本信息 |
|
| 载体名称: | -- |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 4539 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 蠕虫表达式 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | VSA, SV40NLS, gfpN, IVSR, gfpS65C, IVSS, IVST, gfpC, IVSL, decoy |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pPD95_67 was a gift from Andrew Fire (Addgene plasmid # 1490 ; http://n2t.net/addgene:1490 ; RRID:Addgene_1490) |
买家导航
pPD9567载体基本信息
pPD9567载体图谱质粒图谱和多克隆位点信息
pPD9567载体简介
pPD9567载体序列
LOCUS pPD95_67 4532 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pPD95_67
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4532)
AUTHORS Fire A et al
TITLE Fire Lab C. Elegans Vector Kit 1995
REFERENCE 2 (bases 1 to 4532)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..4532
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 146..166
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
primer_bind complement(209..237)
/label=GFP-R
/note="GFP, reverse primer. Does NOT anneal to EGFP"
primer_bind 985..1005
/label=GFP-F
/note="GFP, forward primer. Does NOT anneal to EGFP"
primer_bind complement(1972..1994)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 2094..2113
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind complement(2191..2209)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 2277..2381
/gene="bla"
/label=AmpR promoter
CDS 2382..3242
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(2600..2619)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 3413..4001
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 3902..3921
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 4155..4172
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 4289..4310
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 4325..4355
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4363..4379
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 4368..4390
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 4387..4403
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 4387..4403
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
ORIGIN
1 aagcttgcat gcctgcaggt cgactctaga ggatccccgg gattggccaa aggacccaaa
61 ggtatgtttc gaatgatact aacataacat agaacatttt caggaggacc cttggagggt
121 accgagctca gaaaaaatga ctgctccaaa gaagaagcgt aaggtaccgg tagaaaaaat
181 gagtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga
241 tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa
301 acttaccctt aaatttattt gcactactgg aaaactacct gttccatggg taagtttaaa
361 catatatata ctaactaacc ctgattattt aaattttcag ccaacacttg tcactacttt
421 ctgttatggt gttcaatgct tctcgagata cccagatcat atgaaacggc atgacttttt
481 caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg
541 gaactacaag acacgtaagt ttaaacagtt cggtactaac taaccataca tatttaaatt
601 ttcaggtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg
661 tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc
721 acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagttg taagtttaaa
781 catgatttta ctaactaact aatctgattt aaattttcag aacttcaaaa ttagacacaa
841 cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga
901 tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga
961 tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac
1021 acatggcatg gatgaactat acaaatagca ttcgtagaat tccaactgag cgccggtcgc
1081 taccattacc aacttgtctg gtgtcaaaaa taataggggc cgctgtcatc agagtaagtt
1141 taaactgagt tctactaact aacgagtaat atttaaattt tcagcatctc gcgcccgtgc
1201 ctctgacttc taagtccaat tactcttcaa catccctaca tgctctttct ccctgtgctc
1261 ccacccccta tttttgttat tatcaaaaaa cttctcttaa tttctttgtt ttttagcttc
1321 ttttaagtca cctctaacaa tgaaattgtg tagattcaaa aatagaatta attcgtaata
1381 aaaagtcgaa aaaaattgtg ctccctcccc ccattaataa taattctatc ccaaaatcta
1441 cacaatgttc tgtgtacact tcttatgttt tttacttctg ataaattttt ttgaaacatc
1501 atagaaaaaa ccgcacacaa aataccttat catatgttac gtttcagttt atgaccgcaa
1561 tttttatttc ttcgcacgtc tgggcctctc atgacgtcaa atcatgctca tcgtgaaaaa
1621 gttttggagt atttttggaa tttttcaatc aagtgaaagt ttatgaaatt aattttcctg
1681 cttttgcttt ttggggtttc ccctattgtt tgtcaagatt tcgaggacgg cgtttttctt
1741 gctaaaatca caagtattga tgagcacgat gcaagaaaga tcggaagaag gtttgggttt
1801 gaggctcagt ggaaggtgag tagaagttga taatttgaaa gtggagtagt gtctatgggg
1861 tttttgcctt aaatgacaga atacattccc aatataccaa acataactgt ttcctactag
1921 tcggccgtac gggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
1981 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
2041 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
2101 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
2161 gagaaaatac cgcatcaggc ggccttaagg gcctcgtgat acgcctattt ttataggtta
2221 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
2281 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
2341 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
2401 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
2461 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
2521 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
2581 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
2641 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
2701 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
2761 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
2821 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
2881 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
2941 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
3001 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
3061 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
3121 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
3181 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
3241 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
3301 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
3361 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
3421 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
3481 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
3541 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
3601 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
3661 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
3721 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
3781 aactgagata cctacagcgt gagcattgag aaagcgccac gcttcccgaa gggagaaagg
3841 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
3901 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
3961 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
4021 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
4081 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
4141 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
4201 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
4261 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
4321 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
4381 ttcacacagg aaacagctat gaccatgatt acgccaagct gtaagtttaa acatgatctt
4441 actaactaac tattctcatt taaattttca gagcttaaaa atggctgaaa tcactcacaa
4501 cgatggatac gctaacaact tggaaatgaa at
//
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