| 质粒名称: | pUDP025 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 103874 |
| 存储实验室: | Jean-Marc Daran |
|
载体骨架基本信息 |
|
| 载体名称: | pUDP002 |
| 载体出品公司: | Clontech |
| 空载体大小: | 10046bp |
| 完整质粒大小: | 10237bp |
| 载体类型: | Yeast Expression, CRISPR |
| 筛选标记: | Hygromycin |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | HH-gRNA-HDV targetting ADE2 in Kluyveromyces lactis |
| 物种来源: | Kluyveromyces lactis |
| GenBank编号 | XM_454067 |
| 启动子: | CMV |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | BsaI (destroyed during cloning) |
| 3’克隆位点: | BsaI (destroyed during cloning) |
| 5’测序引物 | ACATCGTAGGTGTCTGGGTGAAC |
| 3’测序引物 | CTTTTCGGTTAGAGCGGATGTGG |
|
文献引用方法 |
|
| 材料和方法部分: | pUDP025 was a gift from Jean-Marc Daran (Addgene plasmid # 103874 ; http://n2t.net/addgene:103874 ; RRID:Addgene_103874) |
| 参考文献部分: | Genome editing in Kluyveromyces and Ogataea yeasts using a broad-host-range Cas9/gRNA co-expression plasmid. Juergens H, Varela JA, Gorter de Vries AR, Perli T, Gast VJM, Gyurchev NY, Rajkumar AS, Mans R, Pronk JT, Morrissey JP, Daran JG. FEMS Yeast Res. 2018 May 1;18(3). pii: 4847887. doi: 10.1093/femsyr/foy012. 10.1093/femsyr/foy012 PubMed 29438517 |
买家导航
pUDP025载体质粒基本信息
pUDP025质粒图谱载体图谱和pUDP025载体序列质粒序列多克隆位点信息
pUDP025质粒载体简介
pUDP025质粒序列载体序列
LOCUS pUDP025 10237 bp DNA circular SYN 15-DEC-2025
DEFINITION Broad-host-range Cas9/gRNA co-expression plasmid with gRNA
targetting ADE2 from Kluyveromyces lactis.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10237)
AUTHORS Juergens H, Varela JA, Gorter de Vries AR, Perli T, Gast VJM,
Gyurchev NY, Rajkumar AS, Mans R, Pronk JT, Morrissey JP, Daran JG
TITLE Genome editing in Kluyveromyces and Ogataea yeasts using a
broad-host-range Cas9/gRNA co-expression plasmid.
JOURNAL FEMS Yeast Res. 2018 May 1;18(3). pii: 4847887. doi:
10.1093/femsyr/foy012.
PUBMED 29438517
REFERENCE 2 (bases 1 to 10237)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Dec 15, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "FEMS Yeast
Res."; date: "2018-05-1"; volume: "18(3). pii: 4847887. doi"; pages:
" 10.1093/femsyr/foy012"
COMMENT Sequence Label: pUDP025
FEATURES Location/Qualifiers
source 1..10237
/mol_type="other DNA"
/organism="synthetic DNA construct"
gene 20..1597
/label=hphMX6
/note="yeast selectable marker conferring hygromycin
resistance"
CDS 368..1396
/codon_start=1
/product="hygromycin B phosphotransferase"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
KE"
CDS 368..1396
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
KE"
CDS 1975..1998
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
CDS 2002..2022
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 2023..6123
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTYKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSITHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS 6127..6147
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
promoter 7066..7732
/gene="S. cerevisiae TDH3"
/label=GAP promoter
/note="promoter for glyceraldehyde-3-phosphate
dehydrogenase; also known as the TDH3 promoter"
primer_bind 7602..7625
/label=GPDpro-F
/note="S. cerevisiae GPD promoter, forward primer"
ncRNA 7739..7775
/product="self-cleaving hammerhead ribozyme (Ferre-D'Amare
and Scott, 2010)"
/label=hammerhead ribozyme
/ncRNA_class="hammerhead_ribozyme"
misc_RNA 7796..7871
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
ncRNA 7876..7943
/product="hepatitis delta virus self-cleaving ribozyme
(Ferre-D'Amare and Scott, 2010)"
/label=HDV ribozyme
/ncRNA_class="ribozyme"
primer_bind complement(7985..8003)
/label=CYC1
/note="CYC1 transcription termination signal, reverse
primer"
CDS complement(join(8437..9228,9229..9297))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9060..9079
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9298..9402)
/gene="bla"
/label=AmpR promoter
rep_origin 9505..10093
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 9994..10013
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 ccagatcatc aataggcacg acatggaggc ccagaatacc ctccttgaca gtcttgacgt
61 gcgcagctca ggggcatgat gtgactgtcg cccgtacatt tagcccatac atccccatgt
121 ataatcattt gcatccatac attttgatgg ccgcacggcg cgaagcaaaa attacggctc
181 ctcgctgcag acctgcgagc agggaaacgc tcccctcaca gacgcgttga attgtcccca
241 cgccgcgccc ctgtagagaa atataaaagg ttaggatttg ccactgaggt tcttctttca
301 tatacttcct tttaaaatct tgctaggata cagttctcac atcacatccg aacataaaca
361 aggatccatg ggtaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga
421 aaagttcgac agcgtgtccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt
481 cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt
541 ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt
601 gcttgacatt ggggaattta gcgagagcct gacctattgc atctcccgcc gtgcacaggg
661 tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcaac cggtcgcgga
721 ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg
781 accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc
841 ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc
901 tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc
961 ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg
1021 gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc
1081 gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc
1141 aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag
1201 cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt
1261 ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg
1321 gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc
1381 gagggcaaag gaataactcg aggacaataa aaagattctt gttttcaaga acttgtcatt
1441 tgtatagttt ttttatattg tagttgttct attttaatca aatgttagcg tgatttatat
1501 tttttttcgc ctcgacatca tctgcccaga tgcgaagtta agtgcgcaga aagtaatatc
1561 atgcgtcaat cgtatgtgaa tgctggtcgc tatactggag ctctcgagaa ccctcacctt
1621 tcgagaggac gatgcccgtg tctaaatgat tcgaccagcc taagaatgtt caacaatcta
1681 taatcagtcc atagtcaaca agagccctca aagttgatat cgtttagtga tctccctcga
1741 gatgagcgga ggatggttgc tttttgggga gccctaagtg gtacgtgcgg cgagcgggat
1801 gtttccccat taggcaaagg acgaccgggt aacccctcga gaaaaaaaat ttttcgccgc
1861 taatctggtg ttatataaag ctccccctgc tcctggaatt tttttccttg tcaactcaca
1921 ccggaaatca aggcatttca ttctgagtag ttcttaaaaa cataatcaac aatggattat
1981 aaagatgacg atgacaaacc tccaaaaaag aagagaaagg tcgataagaa atactcaata
2041 ggcttagata tcggcacaaa tagcgtcgga tgggcggtga tcactgatga atataaggtt
2101 ccgtctaaaa agttcaaggt tctgggaaat acagaccgcc acagtatcaa aaaaaatctt
2161 ataggggctc ttttatttga cagtggagag acagcggaag cgactcgtct caaacggaca
2221 gctcgtagaa ggtatacacg tcggaagaat cgtatttgtt atctacagga gattttttca
2281 aatgagatgg cgaaagtaga tgatagtttc tttcatcgac ttgaagagtc ttttttggtg
2341 gaagaagaca agaagcatga acgtcatcct atttttggaa atatagtaga tgaagttgct
2401 tatcatgaga aatatccaac tatctatcat ctgcgaaaaa aattggtaga ttctacttat
2461 aaagcggatt tgcgcttaat ctatttggcc ttagcgcata tgattaagtt tcgtggtcat
2521 tttttgattg agggagattt aaatcctgat aatagtgatg tggacaaact atttatccag
2581 ttggtacaaa cctacaatca attatttgaa gaaaacccta ttaacgcaag tggagtagat
2641 gctaaagcga ttctttctgc acgattgagt aaatcaagac gattagaaaa tctcattgct
2701 cagctccccg gtgagaagaa aaatggctta tttgggaatc tcattgcttt gtcattgggt
2761 ttgaccccta attttaaatc aaattttgat ttggcagaag atgctaaatt acagctttca
2821 aaagatactt acgatgatga tttagataat ttattggcgc aaattggaga tcaatatgct
2881 gatttgtttt tggcagctaa gaatttatca gatgctattt tactttcaga tatcctaaga
2941 gtaaatactg aaataactaa ggctccccta tcagcttcaa tgattaaacg ctacgatgaa
3001 catcatcaag acttgactct tttaaaagct ttagttcgac aacaacttcc agaaaagtat
3061 aaagaaatct tttttgatca atcaaaaaac ggatatgcag gttatattga tgggggagct
3121 agccaagaag aattttataa atttatcaaa ccaattttag aaaaaatgga tggtactgag
3181 gaattattgg tgaaactaaa tcgtgaagat ttgctgcgca agcaacggac ctttgacaac
3241 ggctctatta cccatcaaat tcacttgggt gagctgcatg ctattttgag aagacaagaa
3301 gacttttatc catttttaaa agacaatcgt gagaagattg aaaaaatctt gacttttcga
3361 attccttatt atgttggtcc attggcgcgt ggcaatagtc gttttgcatg gatgactcgg
3421 aagtctgaag aaacaattac cccatggaat tttgaagaag ttgtcgataa aggtgcttca
3481 gctcaatcat ttattgaacg catgacaaac tttgataaaa atcttccaaa tgaaaaagta
3541 ctaccaaaac atagtttgct ttatgagtat tttacggttt ataacgaatt gacaaaggtc
3601 aaatatgtta ctgaaggaat gcgaaaacca gcatttcttt caggtgaaca gaagaaagcc
3661 attgttgatt tactcttcaa aacaaatcga aaagtaaccg ttaagcaatt aaaagaagat
3721 tatttcaaaa aaatagaatg ttttgatagt gttgaaattt caggagttga agatagattt
3781 aatgcttcat taggtaccta ccatgatttg ctaaaaatta ttaaagataa agattttttg
3841 gataatgaag aaaatgaaga tatcttagag gatattgttt taacattgac cttatttgaa
3901 gatagggaga tgattgagga aagacttaaa acatatgctc acctctttga tgataaggtg
3961 atgaaacagc ttaaacgtcg ccgttatact ggttggggac gtttgtctcg aaaattgatt
4021 aatggtatta gggataagca atctggcaaa acaatattag attttttgaa atcagatggt
4081 tttgccaatc gcaattttat gcagctgatc catgatgata gtttgacatt taaagaagac
4141 attcaaaaag cacaagtgtc tggacaaggc gatagtttac atgaacatat tgcaaattta
4201 gctggtagcc ctgctattaa aaaaggtatt ttacagactg taaaagttgt tgatgaattg
4261 gtcaaagtaa tggggcggca taagccagaa aatatcgtta ttgaaatggc acgtgaaaat
4321 cagacaactc aaaagggcca gaaaaattcg cgagagcgta tgaaacgaat cgaagaaggt
4381 atcaaagaat taggaagtca gattcttaaa gagcatcctg ttgaaaatac tcaattgcaa
4441 aatgaaaagc tctatctcta ttatctccaa aatggaagag acatgtatgt ggaccaagaa
4501 ttagatatta atcgtttaag tgattatgat gtcgatcaca ttgttccaca aagtttcctt
4561 aaagacgatt caatagacaa taaggtctta acgcgttctg ataaaaatcg tggtaaatcg
4621 gataacgttc caagtgaaga agtagtcaaa aagatgaaaa actattggag acaacttcta
4681 aacgccaagt taatcactca acgtaagttt gataatttaa cgaaagctga acgtggaggt
4741 ttgagtgaac ttgataaagc tggttttatc aaacgccaat tggttgaaac tcgccaaatc
4801 actaagcatg tggcacaaat tttggatagt cgcatgaata ctaaatacga tgaaaatgat
4861 aaacttattc gagaggttaa agtgattacc ttaaaatcta aattagtttc tgacttccga
4921 aaagatttcc aattctataa agtacgtgag attaacaatt accatcatgc ccatgatgcg
4981 tatctaaatg ccgtcgttgg aactgctttg attaagaaat atccaaaact tgaatcggag
5041 tttgtctatg gtgattataa agtttatgat gttcgtaaaa tgattgctaa gtctgagcaa
5101 gaaataggca aagcaaccgc aaaatatttc ttttactcta atatcatgaa cttcttcaaa
5161 acagaaatta cacttgcaaa tggagagatt cgcaaacgcc ctctaatcga aactaatggg
5221 gaaactggag aaattgtctg ggataaaggg cgagattttg ccacagtgcg caaagtattg
5281 tccatgcccc aagtcaatat tgtcaagaaa acagaagtac agacaggcgg attctccaag
5341 gagtcaattt taccaaaaag aaattcggac aagcttattg ctcgtaaaaa agactgggat
5401 ccaaaaaaat atggtggttt tgatagtcca acggtagctt attcagtcct agtggttgct
5461 aaggtggaaa aagggaaatc gaagaagtta aaatccgtta aagagttact agggatcaca
5521 attatggaaa gaagttcctt tgaaaaaaat ccgattgact ttttagaagc taaaggatat
5581 aaggaagtta aaaaagactt aatcattaaa ctacctaaat atagtctttt tgagttagaa
5641 aacggtcgta aacggatgct ggctagtgcc ggagaattac aaaaaggaaa tgagctggct
5701 ctgccaagca aatatgtgaa ttttttatat ttagctagtc attatgaaaa gttgaagggt
5761 agtccagaag ataacgaaca aaaacaattg tttgtggagc agcataagca ttatttagat
5821 gagattattg agcaaatcag tgaattttct aagcgtgtta ttttagcaga tgccaattta
5881 gataaagttc ttagtgcata taacaaacat agagacaaac caatacgtga acaagcagaa
5941 aatattattc atttatttac gttgacgaat cttggagctc ccgctgcttt taaatatttt
6001 gatacaacaa ttgatcgtaa acgatatacg tctacaaaag aagttttaga tgccactctt
6061 atccatcaat ccatcactgg tctttatgaa acacgcattg atttgagtca gctaggaggt
6121 gaccctccaa aaaagaagag aaaggtctga gcggttttgt ataactaaat aatattggaa
6181 actaaatacg aatacccaaa ttttttatct aaattttgcc gaaagattaa aatctgcaga
6241 gatatccgaa acaggtaaat ggatgtttca atccctgtag tcagtcagga acccatatta
6301 tattacagta ttagtcgccg cttaggcacg cctttaatta gcaaaatcaa accttaagtg
6361 catatgccgt ataagggaaa ctcaaagaac tggcatcgca aaaatgaaaa catacgttga
6421 aactacggca aaggattggt cagatcgctt catacaggga aagttcggca tcaacatctt
6481 tggataatat cagaatgaga aagaacagat acgcagtacg ttttttggtg agctctttgc
6541 acttctttag ttctttccat caatatcagt tttttaaact tttaggacta aaagtgatgt
6601 ttaacttcaa aatgtttaaa attttgttct tcccgacgtt cattaagaat actaatacac
6661 tttaataatt attttaatat tttgttctaa ataatgactt ttaattaaaa aagataaaat
6721 ataaaaacat cataataact caccagaggt taagaacaaa aaaacaaatt agatatctgc
6781 taatccaata tagttaaatc aatctttcct tggtataatg ggtatattac atatatttca
6841 aggaccgaca ctcctaccaa atatctaaaa tttaccatat taacataaca tgtatataaa
6901 cgtcaaatca taatcagcac taacgtctca cggatcgtat atgccgtagc gacaatctaa
6961 gaactatgcg aggacacgct agggccaagg caaaaagatt ccttgattac gtaagggagt
7021 tagaatcatt ttgaataaaa aacacgcttt ttcagttcga gtttatcatt atcaatactg
7081 ccatttcaaa gaatacgtaa ataattaata gtagtgattt tcctaacttt atttagtcaa
7141 aaaattagcc ttttaattct gctgtaaccc gtacatgccc aaaatagggg gcgggttaca
7201 cagaatatat aacatcgtag gtgtctgggt gaacagttta ttcctggcat ccactaaata
7261 taatggagcc cgctttttaa gctggcatcc agaaaaaaaa agaatcccag caccaaaata
7321 ttgttttctt caccaaccat cagttcatag gtccattctc ttagcgcaac tacagagaac
7381 aggggcacaa acaggcaaaa aacgggcaca acctcaatgg agtgatgcaa cctgcctgga
7441 gtaaatgatg acacaaggca attgacccac gcatgtatct atctcatttt cttacacctt
7501 ctattacctt ctgctctctc tgatttggaa aaagctgaaa aaaaaggttg aaaccagttc
7561 cctgaaatta ttcccctact tgactaataa gtatataaag acggtaggta ttgattgtaa
7621 ttctgtaaat ctatttctta aacttcttaa attctacttt tatagttagt ctttttttta
7681 gttttaaaac accaagaact tagtttcgaa taaacacaca taaacaaaca aattgaaact
7741 gatgagtccg tgaggacgaa acgagtaagc tcgtctttca atacctcaag tgcttgtttt
7801 agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac
7861 cgagtcggtg cttttggccg gcatggtccc agcctcctcg ctggcgccgg ctgggcaaca
7921 tgcttcggca tggcgaatgg gacacaggcc ccttttcctt tgtcgatatc atgtaattag
7981 ttatgtcacg cttacattca cgccctcccc ccacatccgc tctaaccgaa aaggaaggag
8041 ttagacaacc tgaagtctag gtccctattt atttttttat agttatgtta gtattaagaa
8101 cgttatttat atttcaaatt tttctttttt ttctgtacaa acgcgtgtac gcatgtaaca
8161 ttatactgaa aaccttgctt gagaaggttt tgggacaagc ttacgtctca cggatcgtat
8221 atgccgtagc gacaatctaa gaactatgcg aggacacgct agcagctgta ttcacgtaga
8281 cggataggta tagccagaca tcagcagcat acttcgggaa ccgtaggcca ccgagcggcc
8341 gcgattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
8401 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
8461 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
8521 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcggga
8581 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
8641 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
8701 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
8761 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
8821 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
8881 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
8941 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
9001 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
9061 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
9121 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
9181 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
9241 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
9301 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
9361 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
9421 gccacctgtc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
9481 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
9541 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
9601 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
9661 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
9721 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
9781 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
9841 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
9901 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
9961 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
10021 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
10081 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
10141 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtgcggacta
10201 tatgtgaagg catggctatg gcacggcaga cattccg
//
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