JME4599

LOCUS        JME4599               11584 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  NATex_CrisprCas9-yl_RFP: CAS9 vector with Nourseothricin ex marker 
            for gRNA cloning using GoldenGate.
ACCESSION   .
VERSION     .
KEYWORDS    JME4599
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11584)
  AUTHORS   Larroude M, Trabelsi H, Nicaud JM, Rossignol T
  TITLE     A set of Yarrowia lipolytica CRISPR/Cas9 vectors for exploiting 
            wild-type strain diversity.
  JOURNAL   Biotechnol Lett. 2020 Jan 23. pii: 10.1007/s10529-020-02805-4. doi: 
            10.1007/s10529-020-02805-4.
  PUBMED    31974649
REFERENCE   2  (bases 1 to 11584)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11584
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      12..69
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(93..110)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(264..852)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(344..363)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1023..1883)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1646..1665
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1884..1988)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      2078..2121
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     protein_bind    2700..2721
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2736..2766
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2774..2790
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             2808..2819
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             2826..3503
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      3548..3619
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3635..3662
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     protein_bind    4007..4040
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     primer_bind     complement(5197..5219)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5319..5338
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5532..5554
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5546..5563
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5547..5563
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             7176..11279
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             11292..11312
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      11323..11570
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(11339..11357)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 tcttcctgca gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt ttctttaaaa
       61 ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca ctgactcgct gcgctcggtc
      121 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
      181 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
      241 aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga gcatcacaaa
      301 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
      361 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
      421 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
      481 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
      541 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
      601 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
      661 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
      721 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
      781 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
      841 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
      901 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
      961 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     1021 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     1081 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     1141 cccagtgctg caatgatacc gcgcgaccca cgctcaccgg ctccagattt atcagcaata
     1201 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     1261 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     1321 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     1381 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     1441 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     1501 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     1561 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     1621 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     1681 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     1741 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     1801 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     1861 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     1921 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     1981 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     2041 acattaacct ataaaaatag gcgtatcacg aggcagaatt tcagataaaa aaaatcctta
     2101 gctttcgcta aggatgattt ctggaattcg aagagcctct gacctgacgt ccccagttgc
     2161 aaaagttgac acaactctag atctgcttcc aaatatagaa tcataacaag ggttagggtg
     2221 tgattatata atattggtct taattgatgt gctagggctt taaaagttgg ttaaaataac
     2281 gctctaatgc ctttttaata tattgtcttt ttcaaaatct caaatcggac acttcttcgt
     2341 gtatgagact ccattttttg gctccgtcac gtgatatgta ttatcagcta tagtggtgta
     2401 aacaaagttt tttactagct gtaatggcat tttgtcggag tggtaaatcg ccttcttgtt
     2461 gtgcgttcga gttctggact ctgcactggg ctactttgaa aaatacctct aatgcgccga
     2521 tggtttagtg gtaaaatcca tcgttgccat cgatgggccc ccggttcgag agacggggcc
     2581 ggccatctgg gccctagagc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     2641 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     2701 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     2761 atgttgtgtg gaattgtgag cggataacaa tttcacacat actagagaaa gaggagaaat
     2821 actagatggc ttcctccgaa gacgttatca aagagttcat gcgtttcaaa gttcgtatgg
     2881 aaggttccgt taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt cgtccgtacg
     2941 aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg ttcgcttggg
     3001 acatcctgtc cccgcagttc cagtacggtt ccaaagctta cgttaaacac ccggctgaca
     3061 tcccggacta cctgaaactg tccttcccgg aaggtttcaa atgggaacgt gttatgaact
     3121 tcgaagacgg tggtgttgtt accgttaccc aggactcctc cctgcaagac ggtgagttca
     3181 tctacaaagt taaactgcgt ggtaccaact tcccgtccga cggtccggtt atgcagaaaa
     3241 aaaccatggg ttgggaagct tccaccgaac gtatgtaccc ggaagacggt gctctgaaag
     3301 gtgaaatcaa aatgcgtctg aaactgaaag acggtggtca ctacgacgct gaagttaaaa
     3361 ccacctacat ggctaaaaaa ccggttcagc tgccgggtgc ttacaaaacc gacatcaaac
     3421 tggacatcac ctcccacaac gaagactaca ccatcgttga acagtacgaa cgtgctgaag
     3481 gtcgtcactc caccggtgct taataacgct gatagtgcta gtgtagatcg ctactagagc
     3541 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     3601 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
     3661 cgtttatata ggccatctgg gccgggggcg tctctgagct agaaatagca agttaaaata
     3721 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt tttacgtcta
     3781 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggcccagatc
     3841 ctctagagtc gaagcggccg ctatgtctga taaaaggatg taacataggc aagctgctcg
     3901 tgagtgttga gtacgaacct tagatccaaa tcacccgcac ccacggatat acttgcttga
     3961 atatacagta gtatgcggcc gctaggttag ggataacagg gtaatgataa cttcgtatag
     4021 catacattat acgaagttat ggggttggcg gcgcatttgt gtcccaaaaa acagccccaa
     4081 ttgccccaat tgaccccaaa ttgacccagt agcggaccca accccggcga gagccccctt
     4141 caccccacat atcaaacctc ccccggttcc cacacttgcc gttaagggcg tagggtactg
     4201 cagtctggaa tctacgcttg ttcagacttt gtactagttt ctttgtctgg ccatccgggt
     4261 aacccatgcc ggacgcaaaa tagactactg aaaatttttt tgctttgtgg ttgggacttt
     4321 agccaagggt ataaaagacc accgtccccg aattaccttt cctcttcttt tctctctctc
     4381 cttgtcaact cacacccgaa gcacaatgac cactctggat gacaccgctt accgataccg
     4441 aacttccgtt cctggcgatg ccgaggctat tgaggctctg gatggatctt tcaccactga
     4501 caccgttttc cgagtgaccg ctactggcga cggcttcacc ctgcgagagg tgcctgtcga
     4561 ccctcctctc accaaggttt tccctgacga tgagtcggac gatgagtctg acgctggaga
     4621 ggacggcgac cctgactctc gaactttcgt ggcttacggc gacgatggcg acctggccgg
     4681 ctttgtggtc gtttcttact ccggatggaa ccgacgactg accgtggagg acatcgaggt
     4741 cgctcctgag caccgaggtc atggtgtcgg acgagctctg atgggtctgg ctactgagtt
     4801 cgctcgagag cgaggtgctg gccacctgtg gctcgaggtc accaacgtta acgcccctgc
     4861 tattcatgcc taccgacgaa tgggttttac cctgtgtggc ctcgatactg ccctgtacga
     4921 cggaaccgct tccgatggag agcaggccct ctacatgtcg atgccctgcc cttaagtgtc
     4981 tgtggtatct aagctattta tcactcttta caacttctac ctcaactatc tactttaata
     5041 aatgaatatc gtttattctc tatgattact gtatatgcgt tcctctaaga caaatcgaat
     5101 ataacttcgt atagcaggag ttatccgaag cgagtaggga taacagggta atacggggcc
     5161 ctttcgtcac gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccggt
     5221 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     5281 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
     5341 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
     5401 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
     5461 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
     5521 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaattcg agctcggtac
     5581 ccgcttttcg tagataatgg aatacaaatg gatatccaga gtatacacat ggatagtata
     5641 cactgacacg acaattctgt atctctttat gttaactact gtgaggcgtt aaatagagct
     5701 tgatatataa aatgttacat ttcacagtct gaacttttgc agattaccta atttggtaag
     5761 atattaatta tgaactgaaa gttgatggca tccctaaatt tgatgaaagg gggaaccccc
     5821 gggttcgaag gtaccaagga agcatgcggt acccgaattc gcttcgatac gcgtatcgat
     5881 gatacgcgtc catggtgagg tgtctcacaa gtgccgtgca gtcccgcccc cacttgcttc
     5941 tctttgtgtg tagtgtacgt acattatcga gagggttgtt cccgcccacc tcgatccggc
     6001 atgctgaggt gtctcacaag tgccgtgcag tcccgccccc acttgcttct ctttgtgtgt
     6061 agtgtacgta cattatcgag agggttgttc ccgcccacct cgatccggca tgctgaggtg
     6121 tctcacaagt gccgtgcagt cccgccccca cttgcttctc tttgtgtgta gtgtacgtac
     6181 attatcgaga gggttgttcc cgcccacctc gatccggcat gctgaggtgt ctcacaagtg
     6241 ccgtgcagtc ccgcccccac ttgcttctct ttgtgtgtag tgtacgtaca ttatcgagag
     6301 ggttgttccc gcccacctcg atccggcatg ctgaggtgtc tcacaagtgc cgtgcagtcc
     6361 cgcccccact tgcttctctt tgtgtgtagt gtacgtacat tatcgagagg gttgttcccg
     6421 cccacctcga tccggcatgc tgaggtgtct cacaagtgcc gtgcagtccc gcccccactt
     6481 gcttctcttt gtgtgtagtg tacgtacatt atcgagaggg ttgttcccgc ccacctcgat
     6541 ccggcatgct gaggtgtctc acaagtgccg tgcagtcccg cccccacttg cttctctttg
     6601 tgtgtagtgt acgtacatta tcgagagggt tgttcccgcc cacctcgatc cgacatgttg
     6661 aggtgtctca caagtgccgt gcagtcccgc ccccacttgc ttctctttgt gtgtagtgta
     6721 cgtacattat cgagagggtt gttcccgccc acctcgatcc ggcatgcact gatcacgggc
     6781 aaaagtgctt cgatagagag ggggttggcg gcgcatttgt gtcccaaaaa acagccccaa
     6841 ttgccccaat tgaccccaaa ttgacccagt agcggaccca accccggcga gagccccctt
     6901 caccccacat atcaaacctc ccccggttcc cacacttgcc gttaagggcg tagggtactg
     6961 cagtctggaa tctacgcttg ttcagacttt gtactagttt ctttgtctgg ccatccgggt
     7021 aacccatgcc ggacgcaaaa tagactactg aaaatttttt tgctttgtgg ttgggacttt
     7081 agccaagggt ataaaagacc accgtccccg aattaccttt cctcttcttt tctctctctc
     7141 cttgtcaact cacacccgaa ggatccacaa ccacaatgga taagaaatac tccattggcc
     7201 tggacatcgg aaccaactcc gtgggttggg ccgtgatcac cgatgagtac aaggtgccct
     7261 ctaagaaatt caaggtcctg ggcaacaccg accgacactc catcaagaag aacctgatcg
     7321 gcgctctgct cttcgactct ggcgagactg ctgaggccac ccgactgaag cgaaccgctc
     7381 gaagacgata cacccgaaga aagaaccgaa tctgttacct gcaggagatc ttctctaacg
     7441 agatggccaa ggtggacgac tctttcttcc accgactgga ggagtctttc ctggtggagg
     7501 aggacaagaa gcacgagcga caccccatct tcggcaacat cgtggacgag gtggcctacc
     7561 acgagaagta ccccaccatc taccacctgc gaaagaagct ggtggactct accgacaagg
     7621 ccgacctgcg actgatctac ctggccctgg cccacatgat caagttccga ggccacttcc
     7681 tgatcgaggg cgacctgaac cccgacaact ctgacgtgga caagctgttc atccagctgg
     7741 tgcagaccta caaccagctc ttcgaagaga accccattaa cgcttctggc gtggatgcta
     7801 aggccatcct gtctgcccga ctgtctaagt ctcgacgact cgagaacctg attgctcagc
     7861 tccccggaga gaagaagaac ggtctgttcg gaaacctgat tgctctgtcc ctgggtctga
     7921 cccctaactt caagtccaac ttcgatctgg ctgaggacgc taagctgcag ctgtctaagg
     7981 acacctacga cgatgacctg gataacctgc tcgcccagat tggcgaccag tacgccgacc
     8041 tgttcctggc cgccaagaac ctgtctgacg ccatcctgct gtctgacatc ctgcgagtga
     8101 acaccgagat caccaaggcc cccctgtctg cctccatgat taagcgatac gatgagcacc
     8161 accaggatct gaccctcctc aaggctctgg tccgacagca gctgcccgag aagtacaagg
     8221 agattttctt cgaccagtct aagaacggct acgccggcta catcgacggc ggcgcctctc
     8281 aggaggagtt ctacaagttc attaagccca tcctggagaa gatggacgga accgaggaac
     8341 tgctcgtgaa gctgaaccga gaggacctcc tgcgaaagca gcgaaccttc gacaacggct
     8401 ctatccccca ccagatccac ctgggcgagc tgcacgccat cctgcgacga caggaggact
     8461 tctacccctt cctgaaggac aaccgagaga agatcgagaa gatcctgacc ttccgaatcc
     8521 cctactacgt gggacccctg gcccgaggaa actctcgatt cgcttggatg acccgaaagt
     8581 ctgaggagac tattaccccc tggaacttcg aggaggtggt ggataagggc gcctctgctc
     8641 agtctttcat cgagcgaatg accaacttcg acaagaacct ccccaacgag aaggtcctgc
     8701 ccaagcactc tctgctctac gagtacttca ccgtctacaa cgagctcacc aaggtcaagt
     8761 acgtgaccga gggaatgcga aagcccgctt tcctgtctgg agagcagaag aaggctattg
     8821 tggatctgct cttcaagact aaccgaaagg tcaccgtcaa gcagctgaag gaggattact
     8881 tcaagaagat tgagtgtttc gattctgtcg agatctccgg cgtcgaggac cgattcaacg
     8941 cctctctggg tacctaccac gacctgctga agattatcaa ggacaaggat ttcctggata
     9001 acgaggagaa cgaggatatt ctcgaggaca ttgtcctgac cctcaccctg ttcgaggatc
     9061 gagagatgat tgaggagcga ctcaagacct acgctcacct gttcgacgac aaggtgatga
     9121 agcagctgaa gcgacgacga tacaccggct ggggccgact gtctcgaaag ctgatcaacg
     9181 gcatccgaga caagcagtct ggcaagacca tcctggactt cctgaagtct gacggcttcg
     9241 ccaaccgaaa cttcatgcag ctgatccacg acgactctct gaccttcaag gaggacatcc
     9301 agaaggccca ggtgtctggc cagggcgact ctctgcacga gcacatcgcc aacctggccg
     9361 gctctcccgc cattaagaaa ggtatcctgc agaccgtcaa ggtggtcgat gagctcgtca
     9421 aggtgatggg ccgacacaag cccgagaaca ttgtcattga gatggctcga gagaaccaga
     9481 ctactcagaa gggtcagaaa aactcccgag agcgaatgaa gcgaattgag gaaggtatta
     9541 aggagctggg ctcccagatt ctcaaggagc atcccgtgga gaacactcag ctccagaacg
     9601 agaagctgta cctgtactat ctgcagaacg gtcgagacat gtacgtcgac caggagctgg
     9661 atatcaaccg actctccgac tacgatgtgg accacattgt gccccagtcc ttcctgaagg
     9721 acgattctat cgataacaag gtgctgaccc gatccgacaa gaaccgaggc aagtctgaca
     9781 acgtgccctc cgaggaggtg gtcaagaaga tgaagaacta ctggcgacag ctgctgaacg
     9841 ccaagctgat tacccagcga aagttcgaca acctgaccaa ggccgagcga ggcggcctgt
     9901 ctgagctgga caaggccggc ttcatcaagc gacagctggt ggagactcga cagatcacca
     9961 agcacgtggc ccagatcctg gactctcgaa tgaacaccaa gtacgacgag aacgacaagc
    10021 tgatccgaga ggtgaaggtg atcaccctga agtctaagct ggtgtctgac ttccgaaagg
    10081 acttccagtt ctacaaggtg cgagagatta acaactacca ccacgcccac gatgcctacc
    10141 tgaacgctgt cgtgggcacc gccctcatca agaagtatcc caagctggag tccgagttcg
    10201 tctacggcga ctacaaggtc tacgatgtgc gaaaaatgat tgccaagtcc gagcaggaga
    10261 ttggcaaggc taccgccaag tacttcttct actccaacat tatgaacttc ttcaagaccg
    10321 agattaccct ggctaacggc gagattcgaa agcgacccct cattgagact aacggagaga
    10381 ctggtgagat cgtgtgggac aagggacgag acttcgccac cgtgcgaaag gtgctgtcta
    10441 tgccccaggt gaacatcgtg aagaagaccg aggtgcagac cggaggtttc tctaaggagt
    10501 ccatcctgcc caagcgaaac tctgacaagc tgatcgcccg aaagaaggac tgggacccca
    10561 agaagtacgg aggtttcgac tctcccaccg tggcttactc tgtgctggtg gtggccaagg
    10621 tggagaaggg caagtctaag aagctgaagt ctgtgaagga gctgctgggc attaccatca
    10681 tggagcgatc ttctttcgag aagaacccca ttgacttcct ggaggccaag ggatacaagg
    10741 aggtgaagaa agatctgatt atcaagctcc ccaagtactc tctgttcgag ctggagaacg
    10801 gacgaaagcg aatgctggcc tctgccggcg agctgcagaa gggaaacgag ctggccctgc
    10861 cctccaagta cgtcaacttc ctgtacctcg cctcccatta cgagaagctg aagggctctc
    10921 ccgaggataa cgagcagaag cagctcttcg tggagcagca taagcactac ctggacgaga
    10981 tcatcgagca gatttctgag ttctctaagc gagtgatcct ggctgacgcc aacctggata
    11041 aggtgctgtc tgcttacaac aagcaccgag acaagcccat tcgagagcag gctgagaaca
    11101 tcattcacct gttcaccctg accaacctgg gagcccccgc tgccttcaag tacttcgaca
    11161 ccaccatcga ccgaaagcga tacacctcta ccaaggaggt gctggacgcc accctgatcc
    11221 accagtctat caccggcctg tacgagactc gaatcgacct gtctcagctg ggcggcgact
    11281 ctcgagccga ccccaagaag aagcgaaagg tgtaagctag cctcatgtaa ttagttatgt
    11341 cacgcttaca ttcacgccct ccccccacat ccgctctaac cgaaaaggaa ggagttagac
    11401 aacctgaagt ctaggtccct atttattttt ttatagttat gttagtatta agaacgttat
    11461 ttatatttca aatttttctt ttttttctgt acagacgcgt gtacgcatgt aacattatac
    11521 tgaaaacctt gcttgagaag gttttgggac gctcgaaggc tttaatttgc aagcttggcg
    11581 gtct
//