| 质粒名称: | pBK-YE1-BE3 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 85174 |
| 存储实验室: | David Liu |
|
载体骨架基本信息 |
|
| 载体名称: | CMV |
| 载体出品公司: | origene |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 3399 bp |
| 完整质粒大小: | 8532 bp |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | YE1-BE3,rAPOBEC1(W90Y/R126E)-XTEN-Cas9n-UGI-NLS |
| 物种来源: | R. norvegicus (rat); S. pyogenes |
| 插入大小: | 5133 bp |
| 启动子 | CMV |
| 克隆方法: | Ligation Independent克隆 |
| 5' 测序引物 | CMV |
| 3' 测序引物 | TGGTTCTTTCCGCCTCAGAAGC |
|
文献引用方法 |
|
| 材料和方法部分: | pBK-YE1-BE3 was a gift from David Liu (Addgene plasmid # 85174 ; http://n2t.net/addgene:85174 ; RRID:Addgene_85174) |
| 参考文献部分: | Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions. Kim YB, Komor AC, Levy JM, Packer MS, Zhao KT, Liu DR. Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803. 10.1038/nbt.3803 PubMed 28191901 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pBK-YE1-BE3 8532 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Expresses YE1-BE3 in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS pBK-YE1-BE3
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8532)
AUTHORS Kim YB, Komor AC, Levy JM, Packer MS, Zhao KT, Liu DR
TITLE Increasing the genome-targeting scope and precision of base editing
with engineered Cas9-cytidine deaminase fusions.
JOURNAL Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803.
PUBMED 28191901
REFERENCE 2 (bases 1 to 8532)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8532
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 132..335
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 286..306
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 377..396
/label=T7
/note="T7 promoter, forward primer"
promoter 377..395
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 1144..5244
/codon_start=1
/product="nickase mutant of the Cas9 endonuclease from the
Streptococcus pyogenes Type II CRISPR/Cas system"
/label=Cas9(D10A)
/note="generates RNA-guided single strand nicks in DNA due
to the D10A mutation in the RuvC catalytic domain"
/translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS 5257..5505
/codon_start=1
/product="uracil-DNA glycosylase inhibitor from a Bacillus
subtilis bacteriophage (Mol et al., 1995)"
/label=UGI
/translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
CDS 5518..5538
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 5547..5564
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
primer_bind complement(5587..5604)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 5593..5817
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(5888..5904)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5888..5904)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(5901..5923)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 5912..5928
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5936..5966)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5981..6002
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(6119..6136)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6290..6878)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6370..6389)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7049..7909)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7672..7691
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7910..8014)
/gene="bla"
/label=AmpR promoter
primer_bind complement(8093..8112)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 8284..131
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
ORIGIN
1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gagctcagag
421 actggcccag tggctgtgga ccccacattg agacggcgga tcgagcccca tgagtttgag
481 gtattcttcg atccgagaga gctccgcaag gagacctgcc tgctttacga aattaattgg
541 gggggccggc actccatttg gcgacataca tcacagaaca ctaacaagca cgtcgaagtc
601 aacttcatcg agaagttcac gacagaaaga tatttctgtc cgaacacaag gtgcagcatt
661 acctggtttc tcagctatag cccatgcggc gaatgtagta gggccatcac tgaattcctg
721 tcaaggtatc cccacgtcac tctgtttatt tacatcgcaa ggctgtacca ccacgctgac
781 cccgagaatc gacaaggcct gcgggatttg atctcttcag gtgtgactat ccaaattatg
841 actgagcagg agtcaggata ctgctggaga aactttgtga attatagccc gagtaatgaa
901 gcccactggc ctaggtatcc ccatctgtgg gtacgactgt acgttcttga actgtactgc
961 atcatactgg gcctgcctcc ttgtctcaac attctgagaa ggaagcagcc acagctgaca
1021 ttctttacca tcgctcttca gtcttgtcat taccagcgac tgcccccaca cattctctgg
1081 gccaccgggt tgaaaagcgg cagcgagact cccgggacct cagagtccgc cacacccgaa
1141 agtgataaaa agtattctat tggtttagcc atcggcacta attccgttgg atgggctgtc
1201 ataaccgatg aatacaaagt accttcaaag aaatttaagg tgttggggaa cacagaccgt
1261 cattcgatta aaaagaatct tatcggtgcc ctcctattcg atagtggcga aacggcagag
1321 gcgactcgcc tgaaacgaac cgctcggaga aggtatacac gtcgcaagaa ccgaatatgt
1381 tacttacaag aaatttttag caatgagatg gccaaagttg acgattcttt ctttcaccgt
1441 ttggaagagt ccttccttgt cgaagaggac aagaaacatg aacggcaccc catctttgga
1501 aacatagtag atgaggtggc atatcatgaa aagtacccaa cgatttatca cctcagaaaa
1561 aagctagttg actcaactga taaagcggac ctgaggttaa tctacttggc tcttgcccat
1621 atgataaagt tccgtgggca ctttctcatt gagggtgatc taaatccgga caactcggat
1681 gtcgacaaac tgttcatcca gttagtacaa acctataatc agttgtttga agagaaccct
1741 ataaatgcaa gtggcgtgga tgcgaaggct attcttagcg cccgcctctc taaatcccga
1801 cggctagaaa acctgatcgc acaattaccc ggagagaaga aaaatgggtt gttcggtaac
1861 cttatagcgc tctcactagg cctgacacca aattttaagt cgaacttcga cttagctgaa
1921 gatgccaaat tgcagcttag taaggacacg tacgatgacg atctcgacaa tctactggca
1981 caaattggag atcagtatgc ggacttattt ttggctgcca aaaaccttag cgatgcaatc
2041 ctcctatctg acatactgag agttaatact gagattacca aggcgccgtt atccgcttca
2101 atgatcaaaa ggtacgatga acatcaccaa gacttgacac ttctcaaggc cctagtccgt
2161 cagcaactgc ctgagaaata taaggaaata ttctttgatc agtcgaaaaa cgggtacgca
2221 ggttatattg acggcggagc gagtcaagag gaattctaca agtttatcaa acccatatta
2281 gagaagatgg atgggacgga agagttgctt gtaaaactca atcgcgaaga tctactgcga
2341 aagcagcgga ctttcgacaa cggtagcatt ccacatcaaa tccacttagg cgaattgcat
2401 gctatactta gaaggcagga ggatttttat ccgttcctca aagacaatcg tgaaaagatt
2461 gagaaaatcc taacctttcg cataccttac tatgtgggac ccctggcccg agggaactct
2521 cggttcgcat ggatgacaag aaagtccgaa gaaacgatta ctccatggaa ttttgaggaa
2581 gttgtcgata aaggtgcgtc agctcaatcg ttcatcgaga ggatgaccaa ctttgacaag
2641 aatttaccga acgaaaaagt attgcctaag cacagtttac tttacgagta tttcacagtg
2701 tacaatgaac tcacgaaagt taagtatgtc actgagggca tgcgtaaacc cgcctttcta
2761 agcggagaac agaagaaagc aatagtagat ctgttattca agaccaaccg caaagtgaca
2821 gttaagcaat tgaaagagga ctactttaag aaaattgaat gcttcgattc tgtcgagatc
2881 tccggggtag aagatcgatt taatgcgtca cttggtacgt atcatgacct cctaaagata
2941 attaaagata aggacttcct ggataacgaa gagaatgaag atatcttaga agatatagtg
3001 ttgactctta ccctctttga agatcgggaa atgattgagg aaagactaaa aacatacgct
3061 cacctgttcg acgataaggt tatgaaacag ttaaagaggc gtcgctatac gggctgggga
3121 cgattgtcgc ggaaacttat caacgggata agagacaagc aaagtggtaa aactattctc
3181 gattttctaa agagcgacgg cttcgccaat aggaacttta tgcagctgat ccatgatgac
3241 tctttaacct tcaaagagga tatacaaaag gcacaggttt ccggacaagg ggactcattg
3301 cacgaacata ttgcgaatct tgctggttcg ccagccatca aaaagggcat actccagaca
3361 gtcaaagtag tggatgagct agttaaggtc atgggacgtc acaaaccgga aaacattgta
3421 atcgagatgg cacgcgaaaa tcaaacgact cagaaggggc aaaaaaacag tcgagagcgg
3481 atgaagagaa tagaagaggg tattaaagaa ctgggcagcc agatcttaaa ggagcatcct
3541 gtggaaaata cccaattgca gaacgagaaa ctttacctct attacctaca aaatggaagg
3601 gacatgtatg ttgatcagga actggacata aaccgtttat ctgattacga cgtcgatcac
3661 attgtacccc aatccttttt gaaggacgat tcaatcgaca ataaagtgct tacacgctcg
3721 gataagaacc gagggaaaag tgacaatgtt ccaagcgagg aagtcgtaaa gaaaatgaag
3781 aactattggc ggcagctcct aaatgcgaaa ctgataacgc aaagaaagtt cgataactta
3841 actaaagctg agaggggtgg cttgtctgaa cttgacaagg ccggatttat taaacgtcag
3901 ctcgtggaaa cccgccaaat cacaaagcat gttgcacaga tactagattc ccgaatgaat
3961 acgaaatacg acgagaacga taagctgatt cgggaagtca aagtaatcac tttaaagtca
4021 aaattggtgt cggacttcag aaaggatttt caattctata aagttaggga gataaataac
4081 taccaccatg cgcacgacgc ttatcttaat gccgtcgtag ggaccgcact cattaagaaa
4141 tacccgaagc tagaaagtga gtttgtgtat ggtgattaca aagtttatga cgtccgtaag
4201 atgatcgcga aaagcgaaca ggagataggc aaggctacag ccaaatactt cttttattct
4261 aacattatga atttctttaa gacggaaatc actctggcaa acggagagat acgcaaacga
4321 cctttaattg aaaccaatgg ggagacaggt gaaatcgtat gggataaggg ccgggacttc
4381 gcgacggtga gaaaagtttt gtccatgccc caagtcaaca tagtaaagaa aactgaggtg
4441 cagaccggag ggttttcaaa ggaatcgatt cttccaaaaa ggaatagtga taagctcatc
4501 gctcgtaaaa aggactggga cccgaaaaag tacggtggct tcgatagccc tacagttgcc
4561 tattctgtcc tagtagtggc aaaagttgag aagggaaaat ccaagaaact gaagtcagtc
4621 aaagaattat tggggataac gattatggag cgctcgtctt ttgaaaagaa ccccatcgac
4681 ttccttgagg cgaaaggtta caaggaagta aaaaaggatc tcataattaa actaccaaag
4741 tatagtctgt ttgagttaga aaatggccga aaacggatgt tggctagcgc cggagagctt
4801 caaaagggga acgaactcgc actaccgtct aaatacgtga atttcctgta tttagcgtcc
4861 cattacgaga agttgaaagg ttcacctgaa gataacgaac agaagcaact ttttgttgag
4921 cagcacaaac attatctcga cgaaatcata gagcaaattt cggaattcag taagagagtc
4981 atcctagctg atgccaatct ggacaaagta ttaagcgcat acaacaagca cagggataaa
5041 cccatacgtg agcaggcgga aaatattatc catttgttta ctcttaccaa cctcggcgct
5101 ccagccgcat tcaagtattt tgacacaacg atagatcgca aacgatacac ttctaccaag
5161 gaggtgctag acgcgacact gattcaccaa tccatcacgg gattatatga aactcggata
5221 gatttgtcac agcttggggg tgactctggt ggttctacta atctgtcaga tattattgaa
5281 aaggagaccg gtaagcaact ggttatccag gaatccatcc tcatgctccc agaggaggtg
5341 gaagaagtca ttgggaacaa gccggaaagc gatatactcg tgcacaccgc ctacgacgag
5401 agcaccgacg agaatgtcat gcttctgact agcgacgccc ctgaatacaa gccttgggct
5461 ctggtcatac aggatagcaa cggtgagaac aagattaaga tgctctctgg tggttctccc
5521 aagaagaaga ggaaagtcta accggtcatc atcaccatca ccattgagtt taaacccgct
5581 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
5641 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
5701 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
5761 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
5821 ctgaggcgga aagaaccagc tggggctcga taccgtcgac ctctagctag agcttggcgt
5881 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
5941 tacgagccgg aagcataaag tgtaaagcct agggtgccta atgagtgagc taactcacat
6001 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
6061 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
6121 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
6181 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
6241 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
6301 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
6361 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
6421 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
6481 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
6541 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
6601 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
6661 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
6721 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
6781 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
6841 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
6901 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
6961 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
7021 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
7081 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
7141 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
7201 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
7261 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
7321 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
7381 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
7441 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
7501 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
7561 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
7621 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
7681 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
7741 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
7801 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
7861 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
7921 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
7981 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
8041 acgtcgacgg atcgggagat cgatctcccg atcccctagg gtcgactctc agtacaatct
8101 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg
8161 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga
8221 agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc
8281 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
8341 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
8401 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
8461 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
8521 atcaagtgta tc
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载体图谱质粒图谱pdf版和相关资料下载
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